Objective To understand the development status of healthcare-associated infection (HAI)management organizations in Xinjiang region,and effectively improve the management level of HAI in Xinjiang.Methods 101 secondary and above medical institutions in Xinjiang were selected by cluster random sampling method,a retrospec-tive survey was conducted from February 15 to March 31,2016.Development of HAI management organizations in each hospital in 1995,2000,2005,2010,and 2015 were surveyed,statistical analysis of the surveyed results were conducted.Results Of 101 hospitals,22 were tertiary hospitals,79 were secondary hospitals.In 2005 and before, more than 70% of hospitals with >300 beds and 20% of hospitals with< 100 beds carried out HAI management, about 50% of hospitals with >300 beds and less than 5% of hospitals with< 100 beds set up HAI management committees.In 2015,all hospitals carried out HAI management and set up HAI management committees as well as HAI management departments.In 2005 and before,the main HAI management professionals were nursing staff, accounting for about 80%;in 2000 and before,> 50% of full-time professionals received secondary vocational school and below education,in 2005 and after,about 80% of full-time professionals received at least college and above education;in 2000 and before,> 70% of full-time professionals didn’t receive training.In 2015,the percentage of nursing staff engaged in HAI management dropped to 65.90%,and doctors and other professionals both accounted for 17.05%,40.09% of HAI management professionals received university education,92.08% par-ticipated in autonomous region level and above training,1.98% didn’t receive training.Conclusion HAI infection management organizations in Xinjiang region in the past 20 years have developed,the allocation of professionals and professional quality can not meet the needs of HAI infection management in this region.

Objective To investigate the protective effects of dexmedetomidine on myocardial injury of severe burn patients. Methods Seventy-eight cases of severe burn patients were enrolled in this study in our hospital from May 2014 to May 2016. According to the clinical characteristics of treatment, the patients were divided into the control group and the observation group, with 39 cases in each group. Patients in the control group were treated with midazolam sedation on the basis of conventional burns, and patients in the observation group were treated with dexmedetomidine combined with midazolam sedation. The myocardial function-related indicators, heart rate (HR) and mean arterial pressure (MAP) of patients were compared between the 2 groups after treatment. Results (1) The SOD, MDA, CK-MB, cTnI and TNF-αof the two groups after treatment were significantly lower than those before treatment, and the degree of reduction in the observation group was significantly higher than that in the control group (P<0.05);(2) There were significant changes in MAP and HR before and after treatment in the both 2 groups. The decrease s of HR and the degree of MAP elevation in the observation group were significantly higher than those in the control group (P < 0.05, respectively). Conclusions Dexmedetomidine can improve the SOD activity of patients with severe burns, and the decreased expressions of CK-MB, cTnI and TNF-αcan reduce the degree of damage to myocardial tissue microstructure, and can protect the myocardium of patients with severe burns.

[Objective]To compare two preparing procedures for larger chemically acellular nerve allografts (CANA). [Methods]The sciatic nerves of pigs were exposed by a muscle-splitting incision and were isolated free of the underlying fascia. The 60-mm-long segments of the nerve were obtained. They were treated according to the following decellularization processes.In group I,the nerve segments were treated with 7% Triton-100 solution and 7% sodium deoxycholate for two times.In group II,another protocol was created with the detergents Triton X-200,sulfobetaine-16,and sulfobetaine-10 for two times. The degrees of decellularization,activity of laminin,degrees of demyelination,and integrity of the nerve fiber tube were observed under microscope and were assessed by a scoring system.[Results]In both experimental groups the activity of laminin was present and the degrees of decellularization were complete. As for the demyelization of the nerve segments,the myelin sheath in Group II was partially preserved,but it completely disappeared in Group Ⅰ. The structure of the nerve fiber tube in Groups Ⅰ and Ⅱ were not as integral as that in the normal group.[Conclusion]It may be a better method for the larger CANA,to be treated with TritonX-100 and sodium deoxycholate during the decellularization procedure.

OBJECTIVETo investigate the effects of prostate stromal cells from different zones of normal prostate tissue on the growth of prostate cancer cells and their action mechanisms.

METHODSWe extracted stromal cells in the fresh normal prostatic tissue derived from the peripheral zone (PZ) or transitional zone (TZ), amplified them in vitro, and used the supernatants of the cells as conditioned media to culture hormone-resistant prostate cancer DU145 cells. We measured the growth curve of the tumor cells using the CCK8 method, determined the number and viability of the cells by trypan blue staining, evaluated their invasiveness by scratch test, and detected the effects of the stromal cells on the key enzymes in the glycolysis of the tumor cells by Western blot.

RESULTSThe conditioned medium with the PZ-derived stromal cells promoted, while that with the TZ-derived stromal cells inhibited the growth of the tumor cells. The former significantly increased, while the latter markedly decreased the expressions of the key enzymes hexokinase 2 (HK-2), pyruvate kinase 2 (PKM-2), lactate dehydrogenase (LDHA), and pyruvate dehydrogenase (PDH) in the glycolysis of the tumor cells.

CONCLUSIONProstate stromal cells from different zones exert different influences on the growth of tumor cells, which may be associated with their different effects on the glycolysis of tumor cells.

Blotting, Western ; Cell Culture Techniques ; Cell Proliferation ; Culture Media, Conditioned ; Glycolysis ; Humans ; Male ; Prostate ; cytology ; Prostatic Neoplasms ; pathology ; Stromal Cells ; physiology ; Tumor Cells, Cultured

Adult ; Ear Protective Devices ; Female ; Hearing Loss ; prevention & control ; Humans ; Male ; Middle Aged ; Noise, Occupational ; adverse effects ; Occupational Diseases ; prevention & control

Animals ; Brain ; metabolism ; Brain Ischemia ; physiopathology ; Caspase 3 ; metabolism ; Female ; Ischemic Postconditioning ; methods ; Male ; Random Allocation ; Rats ; Rats, Wistar ; Reperfusion Injury ; prevention & control

Arrhythmogenic Right Ventricular Dysplasia ; Humans

BACKGROUND:The rise of tissue engineering has opened up new ways for tissue repair and reconstruction of the urinary tract, and the bladder acellular matrix is a better alternative material for urinary tissue engineering.
OBJECTIVE:To construct the compound of hair fol icle stem cells with heterologous bladder acellular scaffold, and to observe the growth of hair fol icle stem cells on the scaffold.
METHODS:Bladder acellular matrix from New Zealand rabbits were prepared and detected using scanning electron microscopy and Masson staining. Passage 3 hair fol icle stem cells were statical y inoculated into the surface of bladder acellular matrix using secondary sedimentation method. Under inverted microscope, cellgrowth was observed, and cellgrowth curves were drawn. cellgrowth on the scaffold surface was observed through histological detection and scanning electron microscope observation.
RESULTS AND CONCLUSION:Prepared bladder acellular matrix was a white translucent film with fiber mesh structure, and no residual cells were seen. Masson staining results indicated that the bladder acellular matrix had col agen structure, and no obvious residual cells. After culture for 48 hours, hair fol icle stem cells grew wel around the bladder acellular matrix under inverted microscope;1 week later, hair fol icle stem cells extended and adhered to the scaffold surface. These findings indicate that hair fol icle stem cells have a good biocompatibility with the bladder acellular matrix through in vitro culture.

Objective To study the regulatory effect of cefodizime on the T-lymphocyte subsets of peripheral blood in mice with immunological liver injury.Methods The mouse model of immunological liver injury was induced by Bacillus Calmette Guerin and Lipoposaccharide.The study was conducted by using completely random design.The mice with immunological liver injury were divided into thymosin group,cefodizime high-,medium-,low-dose groups,ceftriaxone group and the normal saline group.The six groups were continuously administered agents respectively for 7 days,and T-lymphocyte subsets of peripheral blood in mice were determined and contrasted with those of the normal mice treated with normal saline on the 7th day.Flow cytomytry was used to determine the effects of cefodizime on T-lymphocyte subsets of peripheral blood in mice by using immuno-fluorescence technique.Results The immunological liver injury mice were deficient because their CD3+(%),CD4+(%),CD8+(%) and the ratio of CD4+CD8+ were lower than those of the normal mice.Cefodizime effectively increased CD3+(%),CD4+(%) and the ratio of CD4+CD8+ of the mice with immunological liver injury.Conclusion Cefodizime effectively improves the immune function of the host by regulating the balance between CD4+ cell and CD8+ cell.

[Objective]Electrophoretic analysis of rat chemically extracted acellular nerve allograft(CEAN) protein was performed to investigate the protein of CEAN.Dorsal root ganglia of chicken were cultured onto the surface of CEAN slice to observe the effect of CEAN structure on the fiber regeneration.[Method]The CEAN were perpared according to Sondell method and electrophoresed to observed whether the electrophoresis strip of 28-30kDa(Myelin Protein) exited or not.Dorsal root ganglia of chicken were cultured onto thc surface of CEAN slice and dyed with nerve fiber fluorescence to observe the orientation of nerve fiber growth on the surface of CEAN slice.[Result]Electrophoretic analysis of rat CEAN protein showed that the electrophoresis strip of 28-30 kDa totally disappeared.A large number of nerve fibers grew from DRG along the basement membrane of CEAN.[Conclusion]No myelin protein remained in the CEAN,while the basement membrane structure of CEAN can induce the nerve fiber to grow.