Objective To investigated the significance of serum B7-H3 in differential diagnosis of patients with benign and malignant obstructive jaundice.Methods The B7-H3 levels in 60 patients with obstructive jaundice who were divided into benign (32 patients) and malignant (28 patients) obstructive jaundice group were tested by enzyme-linked immunosorbent assay before and after operation.Results The serum B7-H3 levels of malignant obstructive jaundice group were significantly higher than that of benign group (9334.57 ± 1009.57) pg/mL vs (4450.81 ± 406.92) pg/mL,(P ＜ 0.01).The B7-H3 levels of benign and malignant obstructive jaundice group after operation were significantly lower than those before operation (2531.03 ± 415.64) pg/mL vs (4450.81 ±406.92) pg/mL,(5833.00 ± 2190.87) pg/mL vs (9334.57±1009.57) pg/mL,(P＜0.01).Conclusion B7-H3 participate in the inflammatory immune and tumor immune response,The serum B7-H3 can be used as differential diagnosis index of benign and malignant obstructive jaundice.

Basal-like breast carcinoma is a phenotype of breast cancer which was sub-classified according to gene expression profiling criteria, associated with specific pathological features and biological behaviour. These cancer used to be more aggressive and characterized by the absent expression of ER/PR/CerbB-2(triple negative, TG) however positive for basal cytokeratins(basal CK). We present this review to show its pathological characters, clinical progression, prognosis and response to chemotherapy, and provide convenience for pathologists and surgical colleagues be more aware of this subtype.

Objective:To evaluate the effect of Extracorporeal Shock Wave combined percutaneous injection of enhanced fibrin glue loaded bone growth factor in accelerating the bone nonunion healing and promoting osteogenesis process .Methods: 48 radius model rabbits were randomly divided into four groups.Group A rabbits were treated with ESW;Group B rabbits were treated with ESW combined with FG;Group C rabbits were treated with ESW combined with FG/rhBMP-2/rhbFGF;Group D rabbits were treated with ESW combined with FG/rhBMP-2/rhbFGF/rhVEGF.After a combination therapy of 8 week,conventional X-ray radiography,optical in-spection organization form and biomechanical test were applied to evaluate the therapeutic effect .Results:Compared with the group B , the X-ray group C showed that the fracture line was fuzzy ,bony callus area began to increased ,fracture end range reduced.The histo-morphology showed that cartilage began to form , cartilage cell began to proliferate actively , and new blood vessels were forming surrounding fibrous connective tissue.Biomechanical determination found there was no statistical significance between the two groups ( P>0.05 ).FG/rhBMP-2/rhbFGF+ESW group showed the fracture line was still fuzzy , bony callus disappeared and apparent proliferative bone trabecula ,and biomechanical results showed that there was statistical significance between the two groups compared to the group B(P<0.01).FG/rhBMP-2/rhbFGF/rhVEGF+ESW group showed the fracture line was slightly fuzzy ,apparent proliferative bone trabecula in 4 week,and in 8 week,the fracture line was disappeared and moulding of healing was good.The histomorphology showed that the distribution was filled with fibrous connective tissue and many bone tissue .Biomechanical results showed that it could resistance to bending ,there was statistical significance between the two groups compared to the group C ( P<0.01 ).Conclusion: ESW therapy combined percutaneous injection of enhanced fibrin glue loaded bone growth factor can promote fracture healing and the method of combination is expected to be a good choice of non-surgical treatment of bone nonunion.

Cell Line, Tumor ; Cell Proliferation ; drug effects ; Flow Cytometry ; methods ; Humans ; Point Mutation ; Stomach Neoplasms ; genetics ; pathology ; Tumor Suppressor Protein p53 ; genetics ; pharmacology

Animals ; Male ; Muscle, Skeletal ; metabolism ; Myogenic Regulatory Factors ; metabolism ; Physical Conditioning, Animal ; Rats ; Rats, Sprague-Dawley ; Running

The expressions of Livin and Caspase-9 were detected by immunohistochemical technique in 60 duode-nal adenocarcinoma tissues and 30 adjacent normal tissue. The positive expression rate of Livin in duodenal adeno-carcinoma tissues was significantly higher than that of adjacent normal tissue,and the expression of Caspase-9 was lower than that of adjacent normal tissue ( P<0.01 ) . The expression of Livin was negatively correlated with the ex-pression of Caspase-9 ( P<0.01 ) . The expression of Livin and Caspase-9 was closely correlated with the progress of pathological changes, degree of development,lymph nods metastasis,and clinical stages,but not with sex,age and tumor size ( P>0.05 ) . Livin and Caspase-9 were associated with occurrence and development of duodenal adeno-carcinoma,and the expression of the above two were negatively correlated. Livin and Caspase-9 may be considered as reliable markers for clinical diagnosis,therapy efficacy and the prognosis of duodenal adenocarcinoma.

Objective To observe effect of astragalus injection on levels of blood selenium,T cell immunity function and cytokines with viral myocarditis in children. Methods Fourty two patients with viral myocarditis in children, receiving the therapy of Astragalus injection Observing the levels of blood selenium and cytokine, including interleukin-1 (IL-1), IL-6, tumor necrosis factor-alpha (TNF-?),and including estimation of T lymphocyte subsets and cardiac function too. Results The result showed that in post-treatment, the levels of blood selenium were significantly higher (P

Objective To investigate the effect of fluoride on expression of Runx2 mRNA and protein in bone tissue of rats. Methods Fourteen SD rats were randomly divided into two groups: control group(tap water with fluoride ＜ 0.06 mg/L), and fluorosis group(fluoride 50 mg/L in water). After 4 moths, expressions of both mRNA and protein of Runx2 in rat bone tissue were determined by RT-PCR and Western blotting. Results The results showed that the expression of Runx2 mRNA and protein in fluoride-treated bone tissue were 2.287 ± 0.261 and 0.929 ± 0.229, respectively, both of which were significantly higher than those of control group(0.995 ± 0.123,0.317 ± 0.068, t = 11.85,6.78, P ＜ 0.05). Conclusions Fluoride can increase the expression of Runx2 mRNA and protein in bone tissue of rats, and Runx2 may be involved in the pathogenesis of bone injury caused by fluoride.

Objective To investigate the decreasing pattern of activities of daily living (ADL) in patients with dementia. Methods A total of 204 patients with dementia were collected,among which 106 cases were with Alzheimer's diseases(AD),54 cases with vascular dementia(VD),44 cases with other types of dementia.According to the scores of mini-mental state examination(MMSE),mild,moderate and severe dementia were 42 cases,37 cases and 125 cases,respectively.All of them were assessed by Barthel index(BI) and compared among degrees of dementia,between AD and VD,and levels of education. Results The most reserved items were bowel movement (48.5 ％),urination (44.1 ％) and grooming (40.2％),while the most vulnerable items were climbing stairs (25.5％) and bathing(18.1％).With increasing impairment of cognition,the scores of BI were getting decreasing. There were significant differences among severe [(23.20 ± 29.27)scores],moderate[(65.54 ± 26.35) scores] and mild [(87.38 ± 22.69) scores] groups (x2 =99.686,P =0.000). Basic ADL were more reserved in AD (16.98％-53.77％) compared with VD (1.85％-33.33％) patients.No significant differences were found(x2 =3.653,P=0.161)among patients with education of primary school and below [(40.1 ± 39.0) scores],middle school[(45.3 ± 39.6 ) scores],and high school and above[(53.1 ± 37.7) scores]. Conclusions Among patients with dementia,bowel control,bladder control and grooming are well reserved,climbing stairs and bathing are vulnerable functions.Basic activities of daily living may be affected by cognitive status and the types of dementia but not levels of culture.

Objective　 To promote the therapeutic effect about the primary hypertension connected psychological factor through interventing anxiety emotion.Method　All primary hypertension patients with anxiety emotion were divided into two groups randomly.Each group was treated by lotension for 8 weeks,While group 1 combined antianxiety drugs Deanxit Result　The effective rate of two groups were 93.94%(group 1), 60.61%(group 2).Conclusion　 The therapeutic effect of group 2 is lower than that of group 1.