1.Effects of SV heat-resisting protein on the microglial cells in MPTP-treated mice.
Sheng-Ming YIN ; De-Qin YU ; Ning AN
Chinese Journal of Applied Physiology 2009;25(1):79-90
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine
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Animals
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Female
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MPTP Poisoning
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drug therapy
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pathology
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physiopathology
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Male
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Materia Medica
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therapeutic use
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Mice
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Mice, Inbred C57BL
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Microglia
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drug effects
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pathology
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Parkinson Disease, Secondary
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chemically induced
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drug therapy
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physiopathology
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Scorpion Venoms
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chemistry
2.The effect of tranilast on the down-regulation of beta-adrenoceptors
Yu YANG ; Kaisheng YIN ; Ming YANG
Chinese Pharmacological Bulletin 1987;0(02):-
The effect of tranilast on the down- regulation of beta- adrenoceptor agonist was investigated. Male guinea pigs were divided into 3 groups, one group is control. Others were received injection of terbutaline or terbutaline plus tranilast for successive 5 days. The radioli-gand binding assays for beta-adrenoceptors in the lung membranes of the guinea pigs were per-formed. The results showed the numbers of maximal binding sites (Bmax) of beta-adrenoceptors in terbutaline group was significantly less than that in control group ( P 0. 05).The affinity ( Kd) of beta-adrenoceptors both in terbutaline group and in terbutaline plus tranilast group was higher than that in control (P
3.Minimal and optimal concentration of ropivacaine for epidural anesthesia in mastectomy
Gang YIN ; Yishu LIU ; Shuming ZHANG ; Ming YU ; Qixiang SHEN
Cancer Research and Clinic 2013;(3):195-197,213
Objective To investigate the minimal and optimal concentration of ropivacain for epidural anesthesia in patients with breast cancer.Methods 103 patients with breast cancer ASA Ⅰ-Ⅱ undergoing elective radical mastectomy were conducted with epidural anesthesia.T2-3 were selected as a puncture site.A catheter was inserted into the epidural space in a cephalic direction for 3.5-4.0 cm.The concentration of ropivacain was produced by the test of up-down sequential allocation technique.20-25 ml of 0.20 % ropivacaine was given after a test of 0.20 % 5 ml ropivacaine.If the anesthesia was effective (VAS≤3),the next concentration was down 0.01%,while the VAS was more than 5,the next concentration was up 0.01%.The anesthesia effect and segments of block were measured with acupuncture.Blood pressure (NIBP),heart rates(HR),blood oxygen saturation (SPO2),blood gas and VAS were monitored during operation.Nasal catheter oxygen inhalation was used in routine method.Results The median effective concentration (EC50) of ropivacaine was 0.10 % determined by the formula of dixon and massey.The 95 % confidence intervals was 0.1022 %-0.1065 %.The least and the best analgesic concentration of ropivacaine for the upper thoracic epidural block was 0.14 % and 0.17 %-0.18 % respectively.Conclusion The minimal and the optimal analgesic concentration of ropivacaine for the upper thoracic epidural block are 0.14 % and 0.17 %-0.18 %respectively,and which provides a safe and rational use of ropivacaine for the clinic.
5.Asiatic acid induces apoptosis in T98 G human glioblastoma cells by changing autophagy
Huan LI ; Fuqin GUAN ; Yu CHEN ; Min YIN ; Hao SUN ; Ming WANG ; Xu FENG ; Yu SHAN
Chinese Pharmacological Bulletin 2015;(10):1363-1367,1368
Aim To investigate the effect of asiatic acid on apoptosis and autophagy in human glioblastoma T98G cells. Methods MTT colorimetry was employed to assay the cellular proliferating activity. The fluores-cence microscope and Hoechst 33258 staining were used to detect the morphological changes. The cell ap-optosis and autophagy were analyzed by flow cytometry with Annexin-V/7-AAD and MDC staining respective-ly. The expressions of associated proteins were detected by Western blot to analyze the mechanism of apoptosis and autophagy. Results MTT assay showed that the growth of T 9 8 G cells was inhibited by asiatic acid ( IC50 =46. 3 μmol · L-1 ) . Annexin V/7-AAD stai-ning and Western blot revealed that asiatic acid in-duced apoptosis in T98 G cells by reducing the expres-sion of Akt, decreasing the mitochondrial membrane potential, and increasing the expression of Caspase-3. MDC staining and Western blot showed that the per-centage of MDC-positive cells was decreased and the expressions of Beclin-1 , LC3-II and Atgs were inhibi-ted by asiatic acid treatment. 5 μmol·L-1 chloroquine was used to up-regulate the expressions of LC3-Ⅱand Beclin-1 . Asiatic acid-inhibited autophagy was blocked and the total apoptotic rate was reduced remarkably. Conclusion Asiatic acid suppresses T98 G cells pro-liferation by inducing apoptosis and inhibiting cell au-tophagy, and the very role of inhibiting autophagy could promote apoptosis to a certain extent.
6.Learning resource exploration of anesthesiology postgraduate
Yu-qing WU ; Cheng-hua ZHOU ; Yong-ping WU ; Xiao-xing YIN ; Li-cai NG ZHA ; Yin-ming ZENG
Chinese Journal of Medical Education Research 2011;10(11):1296-1298
Thorough excavation and artful utilization of various kinds concrete and invisible learning resources contribute to the cultivation of excellent postgraduates.In postgraduate education of anesthesiology Xuzhou Medical College utilizes time,network,technique platform,research outcome,self-potentiality and clinical patients resources,which produces an active effect and has important instructional significance.
7.Construction of Lentiviral Expression Vector Containing Extracellular Domain of Human Hepatocyte Growth Factor Receptor and Its Expression in 293T Cell.
Jia GUO ; Yanxin YIN ; Ming JIANG ; Lihua YU ; Yun JIANG ; Guiqing LI ; Jianmin FANG
Journal of Biomedical Engineering 2015;32(2):400-404
This research aims to construct a lentiviral expression vector carrying the extracelluar domain (ED) of human hepatocyte growth factor receptor (C-Met), and to express it in transfected 293T cells. The extracellular domain of C-Met was amplified by RT-PCR, ligated with lentiviral expression vector p RRL-CMV-ED, and then expressed in 293T cell line. The expressed protein was purified and identified by RT-PCR and Western blot. The enzyme digestion and sequence analysis showed that the lentiviral expression vector p RRL-CMV-ED was constructed correctly. The size of amplified genes was about 2 700 bp. The purified protein with Ni-affinity column was about 105 kD analyzed by SDS-PAGE. The Western blot and ELISA results showed that the expressed protein which could bind to HGF specifically was the extracelluar domain of human hepatocyte growth factor receptor. This research may lay a foundation for further study of anti-C-MET monoclonal antibody and neutralizing antibody.
Genetic Vectors
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HEK293 Cells
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Humans
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Lentivirus
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Proto-Oncogene Proteins c-met
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genetics
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metabolism
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Transfection
8.Design, synthesis and biological evaluation of novel 3-(2-oxo-2-substituted acetamido)benzamides as PARP-1 inhibitors.
Yu-wen YIN ; Ming JI ; Ran CAO ; Xiao-guang CHEN ; Bai-ling XU
Acta Pharmaceutica Sinica 2015;50(6):738-745
Poly(ADP-ribose)polymerase-1 (PARP-1) plays a significant role in the DNA repair process by catalyzing the transfer of ADP-ribose from NAD+ to its receptors. It is a promising anticancer drug target and many PARP-1 inhibitors have been developed and used in the clinical trial. In this work, a series of 3-(2-oxo-2-substituted acetamido)benzamides have been synthesized and their inhibitory activities against PARP-1 were evaluated. Of all the tested compounds, six compounds displayed inhibitory activities with IC50 values ranging from 0.23 to 5.78 µmol.L-1 . The binding pose of compound 5a was predicted using molecular docking to facilitate further structural modification.
Antineoplastic Agents
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Benzamides
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chemistry
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DNA Repair
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Drug Design
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Humans
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Molecular Docking Simulation
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Poly(ADP-ribose) Polymerase Inhibitors
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chemical synthesis
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chemistry
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Poly(ADP-ribose) Polymerases
9.Expression and identification of immune activity of cytosolic superoxide dismutase of Schistosoma japonicum
Chuanxin YU ; Xuren YIN ; Jian LI ; Yongliang XU ; Ming XU ; Qi GAO ; Yousheng LIANG
Chinese Journal of Schistosomiasis Control 1989;0(03):-
Objective To express the cytosolic superoxide dismutase(SOD)of Schistosoma japonicum and analyze its antigenicity.MethodsThe DNA sequence of Schistosoma japonicum gene of cytosolic SOD was amplified by reverse transcription polymerase chain reaction(RT-PCR)and subcloned into the expression vector pGEX-4T-3 to construct a recombinant plasmid Sj SOD-pGEX-4T-3.This recombinant plasmid was transformed into component cell of E.coli BL21(DE3).The fusion protein of GST-SOD was expressed by inducing with IPTG and purification by affinity chromatography.The specific antiserum was prepared by immunizing the BALB/c mouse with GST-SOD fusion protein,and the immnuogenicity of GST-SOD was investigated by Western blot analysis.ResultsThe gene of cytosolic SOD was amplified successfully and subcloned into expression vector pGEX-4T-3 forming the recombinant expression plasmid Sj SOD-pGEX-4T-3.The fusion protein GST-SOD was expressed after the recombinant containing Sj SOD-pGEX-4T-3 being induced by IPTG.Immunizing the BALB/c mouse with the fusion protein GST-SOD produced high titer specific antiserum of 1∶12800 and the fusion protein GST-SOD was recognized by sera of severe infection rabbits.ConclusionsThe cytosolic SOD has been expressed successfully and has a preferable immunogenicity.
10.Detecting Hepatitis B Virus Deoxyribonucleic Acid Gene in Breast Milk by Nested Polymerase Chain Reaction
xiao-mei, YU ; wei-hong, HU ; jian-ming, DU ; yin, ZHANG
Journal of Applied Clinical Pediatrics 2004;0(09):-
Objective With the method of detecting hepatitis B virus deoxyribonucleic acid(HBV-DNA) gene in breast milk,in ordered to diagnose whether there is HBV in breast milk in those mothers whose HBsAg are positive.Methods Collected breast milk from 187 mothers whose HBsAg were positive,and complified HBV-N gene in breast milk by Nested-Polymerase chain reaction (Nested-PCR).Results Nested-PCR could detect HBV-DNA in breast milk,and the positive rate was 3.2%.Conclusion The method of detecting HBV-N gene in breast milk by Nested-PCR can detect HBV-DNA in breast milk,and it can be an laboratory evidence for whether breast feeding or not.