No abstract available.
Graves Disease* ; Humans ; Thyroid Gland*

No abstract available.
Trigger Points*

PURPOSE: The cell cycle control system is necessary for the normal growth and differentiation of cells. The purposes of this study were to compare CD99 expression with a known intracellular marker of a specific cell cycle and to evaluate the potential of CD99 as surface marker for this cell cycle. METHODS: We induced arrest of the cell cycle in fetal lung fibroblast by contact inhibition or serum deprivation from culture media. We activated peripheral blood lymphocytes with the treatment of phytohemagglutinin (PHA) and interleukin-2 (IL-2). Next, we synchronized the cell cycle of peripheral blood lymphocytes to the late G1 phase with rapamycin. According to their CD99 expression, the peripheral blood lymphocytes were separated by magnetic bead and analyzed by Western blotting. RESULTS: CD99 expression in fetal lung fibroblast rapidly decreased in cell cycle arrest and recovered soon after G1 activation of the cell cycle. By analyzing chronologic changes of CD99 expression and PI-histogram, we found CD99 expression decreased after passing the G1 checkpoint. G1/S transition was interrupted by potent immunosuppresant, rapamycin. IL-2 receptor remained high after rapamycin treatment in the activated lymphocytes, whereas CD99 expression and propium iodide decreased as compared with the same condition without rapamycin. This suggested that CD99 expression was decreased in the late G1 phase. Retinoblastoma gene (Rb) and CDK-2 are necessary for G1/S transition. We found both of these in CD99+ lymphocyte through Western blotting only. Cyclin B, which has an important role in S/G2/M transition, was only found in CD99-activated lymphocytes. CONCLUSION: CD99 may be a G1 phase specific surface marker.
Blotting, Western ; Cell Cycle Checkpoints ; Cell Cycle* ; Contact Inhibition ; Culture Media ; Cyclin B ; Fibroblasts ; G1 Phase ; Genes, Retinoblastoma ; Interleukin-2 ; Lung ; Lymphocytes ; Receptors, Interleukin-2 ; Sirolimus

The aim of this study was to show the feasibility and potential advantages of using the new intraoral distraction device for alveolar ridge augmentation. Four adult mongrel dogs were used. We designed intraoral distraction device for augmentation of vertical height of the edentulous ridge. The left upper and lower premolars and first molars were extracted and an alveoloplasty was performed to simulate an atrophic ridge. After 12 weeks of healing, an osteotomy was made and the intraoral distraction device was applied in order to distract alveolar segment upward. A permucosal pin was exposed intraorally. Latency period was allowed for 7 days before distraction began. The distraction device was activated with a rate of 1.0 mm/day for 9 days. At 2 weeks after completion of the distraction, the device was removed. At 8 weeks after distraction, the animals were sacrificed. Clinical, radiographic, and histologic examinations were performed. Macroscopically, no significant abnormalities such as infection were occurred in the distracted area. The average distracted distance was 7.6+/-1.4 mm and new bone was formed in the distracted gap in all animals. Bone remodeling without crestal bone resorption was observed in the distracted area. In several animals, fibrous tissue was present in the buccal cortical bone area. These results suggest that the intraoral diatraction device may have the potential for use in augmentation of the atrophic edentulous ridge.
Adult ; Alveolar Process* ; Alveolar Ridge Augmentation* ; Alveoloplasty ; Animals ; Bicuspid ; Bone Remodeling ; Bone Resorption ; Dogs* ; Humans ; Latency Period (Psychology) ; Molar ; Osteogenesis, Distraction ; Osteotomy

Adult ; Alveolar Process ; Animals ; Bicuspid ; Bone Resorption ; Dogs ; Humans ; Latency Period (Psychology) ; Molar ; Osteotomy

Congenital unilateral renal agenesis is a rare congenital anomaly. Furthermore, renal agenesis associated with neuroblastoma and cryptorchism is more rare in incidence. Recently we experieced a case of Rt. renal agenesis combined with cryptorchism and neuroblastoma. We diagnosed it with IVP and selective renal angiography and confirmed it with explolaparotomy and biopsy. We reviewed the literatures on renal agenesis, cryptorchism and neuroblastoma, and discussed possible embryological relationships between them and other combined congenital anomalies.
Angiography ; Biopsy ; Cryptorchidism* ; Embryology ; Incidence ; Male ; Neuroblastoma*

Purpose: Although Mycoplasma pneumoniae (M. pneumoniae) infection can cause wheezing in non-asthmatic children, the mechanisms of this symptom remain unclear. Vascular endothelial growth factor (VEGF) is a major mediator of angiogenesis and vascular permeability, and is also known to be elevated in cases of chronic pulmonary disease such as asthma. We hypothesized that VEGF may increase in children with acute M. pneumoniae pneumonia and wheezing. Methods: Nine patients with clinical and laboratory evidence of acute M. pneumoniae pneumonia were enlisted from children admitted to Korea University Hospital. They had had more than one episode of wheezing during the illness, which was confirmed by a physician; they comprised the wheezer group. The individuals with M. pneumoniae pneumonia without wheezing were 63 in number, and they comprised the non-wheezer group. Patients with a history of asthma or who had received asthma medications were excluded. Serum concentrations of VEGF, total IgE, eosinophil cationic protein (ECP), and peripheral blood eosinophil counts were measured. Results: The serum VEGF concentrations were higher in the wheezer group (mean+/-SD; 650.2+/-417.9 pg/mL) than in the non-wheezer group (376.5+/-356.2 pg/mL, P=0.049). M. pneumoniae antibody (1:1,380 vs. 1:596, P=0.048) and serum total IgE (591.8 IU/mL vs. 162.2 IU/mL, P=0.032) were higher in the wheezer group than in the non-wheezer group. There were no differences between the two groups in terms of serum ECP concentration or blood eosinophil count. Conclusion: In the presence of wheezing, serum VEGF concentrations were higher in the children with M. pneumoniae pneumonia. This finding suggests that VEGF may associate with wheeze-related symptoms in children with acute M. pneumoniae pneumonia.
Asthma ; Capillary Permeability ; Child ; Eosinophil Cationic Protein ; Eosinophils ; Humans ; Immunoglobulin E ; Korea ; Lung Diseases ; Mycoplasma ; Mycoplasma pneumoniae ; Pneumonia ; Pneumonia, Mycoplasma ; Respiratory Sounds ; Vascular Endothelial Growth Factor A

A 56-year-old man had five nail gun-shots on his skull due to attempted suicide and was transferred to the emergency room. Because the nail head played a role as a brake, the launched nail made a hole in the skull but did not entirely pass through it. If major artery or sinuses are not involved, cautious retrieval after a small scalp incision can be performed and prophylactic antibiotics be administered for treatment.
Anti-Bacterial Agents ; Arteries ; Craniocerebral Trauma* ; Emergency Service, Hospital ; Head ; Humans ; Middle Aged ; Scalp ; Skull ; Suicide, Attempted

An aneurysm of the distal superior cerebellar artery (SCA) is a highly rare disease. Fusiform aneurysms of the distal SCA are particularly challenging to treat. Clipping, trapping with or without bypass using microsurgery or endovascular treatment (EVT) were used to treat this condition. We describe the case of fusiform distal SCA aneurysms treated successfully with endovascular coiling with a 3-month follow-up. A 39 year-old male was presented with subarachnoid hemorrhage (SAH) and a 15 mm fusiform aneurysm of the ambient segment of the left distal SCA. EVT for parent artery occlusion and packing of the aneurysm was done. Left sixth nerve palsy appeared after 1 day of EVT. The symptom completely recovered within 1 week of the post-procedural period. No neurological deficit was seen during the clinical 3-month follow-up. EVT of fusiform distal SCA aneurysms with coils is a safe and feasible option to manage this rare condition. However, the treatment options must be carefully selected depending on the neurologic condition, development of collateral circulation, and configuration of the dissection.
Abducens Nerve Diseases ; Aneurysm* ; Arteries* ; Collateral Circulation ; Endovascular Procedures ; Follow-Up Studies ; Humans ; Intracranial Aneurysm ; Male ; Microsurgery ; Parents ; Rare Diseases ; Subarachnoid Hemorrhage

No abstract available.
Humans* ; Multiplex Polymerase Chain Reaction* ; Papilloma*