Adult ; Coronary Occlusion ; therapy ; Extracorporeal Membrane Oxygenation ; Humans ; Male ; Treatment Outcome

Objective: This work aimed to construct stable MCF-7 cell sublines from which staphylococcal nuclease domain con-taining 1 (SND1) expression was interfered to analyze the effect of SND1 silencing on the proliferation and metastasis of MCF-7 cells. Methods: The lentivirus that could mediate SND1 silencing was prepared. MCF-7 cells were infected with the lentiviruses to construct stable sub-cell lines. Quantitative real-time polymerase chain reaction and Western blot analysis were employed to determine SND1 ex-pression level. MTS, wound healing, and transwell assays were applied to analyze the effect of SND1 silencing on the proliferation, mi-gration, and invasion of MCF-7 cells. Results: A lentivirus expression vector that contains sequences encoding shRNAs targeting SND1 and an shRNA negative control were successfully established. The lentiviruses (LV-SH1, LV-SH2, LV-SH3, and 和 LV-NC) were then collected and packaged. Stabilized MCF-7 sublines were prepared through infection with lentiviruses. The most efficient MCF-7 stable cell subline, MCF-SH3, was selected for SND1 silencing. Compared with the control cell, the proliferation, migration, and inva-sion potential of MCF-SH3 were significantly decreased. Conclusion: SND1 could promote the proliferation, migration, and invasion of breast cancer cells. Thus, silencing SND1 expression will inhibit such proliferation, migration, and invasion. These results indicated that the unusual expression of SND1 is associated with breast cancer and may participate in cancer progression by affecting prolifera-tion, migration, and invasion.

Objective To observe the effect of montelukast sodium and ambroxol oral solution in treatment respiratory symptoms caused by mycoplasma infection in children.Methods 147 children who were infected by mycoplasma pneumonia with lower respiratory tracts symptom were randomly divided into A,B,C three groups:group A was control group,group B was treated by montelukast sodium,and group C was treated by montelukast sodium and ambroxol oral solution.Coughing,symptomatic relief of pant were observed in each group.Results After 7 days treatment,the total effective rate of three groups was A group 79.5％,B group 95.7％,C group 96.4％.Compared with group A,the coughing and panting time in group B and group C were significantly shorter(x2 =5.61,9.54,all P ＜0.05),The rate of coughing and panting complete remission in group C was higher compared with group B (x2 =5.39,P ＜ 0.05),but there was no statistificantly significant difference in the total efficency between the two groups.Conclusion Montelukast sodium and ambroxol oral solution can effectively improve the symptoms of respiratory tract infection caused by mycoplasma pneumoniae.

Objective To investigate the inhibitory effects of Propionibacterium acnes (P.acnes) by 22 effective components of traditional Chinese medicines. Methods The susceptibility tests of P.acnes to 22 effective components of traditional Chinese medicines were performed. Results P.acnes was highly sensitive to both eugenol and cineole, and moderately sensitive to baicalin, emodin and others. The number of bacteria per oil field decreased obviously under microscopy and bacteriolysis was found after the treatment with those components. Conclusion Eugenol, cineole and other components of traditional Chinese medicines can inhibit P.acnes in vitro.

The precise measurement in lens thickness in vivo, provides great application value for intraocular accommodation and ametropia development mechanism research.And it has great clinical significance for the diagnosis and treatment of glaucoma and cataract.Currently, many ultrasonic methods and optical methods are used in measuring lens thickness.The measurement principles, advantages, disadvantages and the accuracy of the instruments are summarized in this paper.Among these methods, Orbscan II, Pentacam, Lenstar and AS-OCT can be used to measure lens thickness instead of A-scan.More important is the fact that UL-OCT can dynamically monitor the change of the lens thickness with intraocular accommodation.Choosing an instrument with higher measuring accuracy to examine the lens thickness, can provide more accurate and convincing lens thickness data for clinical and scientific research.

Objective: To explore the influence of adenovirus carrying Galectin-9 gene on the survival of cardiac transplant and the differentiation and proliferation of CD4+ CD25+ Treg cells in mice receiving heterotopic heart transplantation. Methods: Neck heterotopic heart transplantation models were established with male BALB/c→C57BL/6 mice by Cuff method, and they were randomly divided into 3 groups (n=10): control group (received 0.9% sodium chloride solution after operation), adenovirus group, and pAdeasy™ Galectin-9 treatment group. The survival time of the graft hearts was recorded. The spleens of mice were collected when the graft hearts stopped beating or 14 days after operation) the mononuclear cells were isolated and the proportion of CD4+ CD25+ Treg cells was determined by flow cytometry. Treg cell related cytokines (IL-10, TGF-β1) in the peripheral blood were assayed by ELISA. Results: The cardiac allografts in pAdeasy™ Galectin-9 group survived significantly longer than those in the other two groups (P<0.01), and the proportion of CD4+ CD25+ Treg cells in CD4+ T cells was significantly higher (P<0.01). The levels of IL-10 and TGF-β1 were increased in pAdeasy™ Galectin-9 group compared with control group and adenovirus group (P < 0.01). Conclusion: pAdeasy™ Galectin-9 can promote the proliferation and differentiation of CD4+ CD25+ Treg cells in vivo, and can greatly improve the survival time of cardiac allograft after heart transplantation in mice.

Objective To investigate the effect of sufentanil on anoxia/rexogenation (A/R)-induced injury to H9c2 cells incubated in high-glucose culture medium. Methods The H9c2 cells were cultured in low-glucose DMEM/F12 culture medium supplemented with 10% fetal bovine serum. The cells were seeded in 96-well or 6-well plates and randomly divided into 5 groups ( n = 12 wells each): normal glucose control group (group NC),high-glucose control group (group HC), high-glucose + A/R group (group HA/R), high-glucose + sufentanil +A/R group (group HSA/R), high glucose + naloxone + A/R group (group HNA/R). The cells were exposed to 95 % N2-5 % CO2 in an incubator at 37 ℃ for 3 h followed by 3 h reoxygenation. In group NC, the cells were incubated in low-glucose culture medium for 48 h. In group HC, the cells were incubated in high-glucose culture medium for 48 h. In group HA/R, the cells were incubated in high-glucose culture medium for 48 h before anoxia.In group HSA/R, after the cells were incubated in high-glucose culture medium for48 h, sufentanil was added to the culture medium with the final concentration of 10-9 mol/L at 15 min before anoxia. In group HNA/R, after the cells were incubated inhigh-glucose culture medium for 48 h, naloxone was added to the culture medium with the final concentration of 10-6 mol/L, 10 min later sufentanil was added with the final concentration of 10-9 mol/L at 15 min before anoxia. The cell viability was measured by MTT assay. The amount of lactic dehydrogenase (LDH) released in the supernatant and superoxide dismutase (SOD) activity were measured. Results The cell viability and SOD activity were significantly lower, while the amount of LDH released was significantly higher in the other groups than in group NC, in groups HA/R and HNA/R than in group HC, and in group HNA/R than in group HSA/R (P ＜ 0.01 ). The cell viability and SOD activity were significantly higher, while the amount of LDH released was significantly lower in group HSA/R than in group HA/R ( P ＜ 0.01 ). There was no significant difference in the parameters mentioned above between group HNA/R and group HA/R ( P ＞ 0.05). Conclusion Sufentanil can attenuate A/R-induced injury to H9c2 cells with high-glucose incubation, and the mechanism may be related to the activation of opioid receptors.

AIM:To identify and analyze tyrosine-phosphorylated proteins regulated by protein tyrosine phos-phatase-like A domain containing protein 1 ( PTPLAD1) in colon cancer cells by phosphoproteomics.METHODS: The expression of PTPLAD1 in colon cancer cell line HCT-116 was knocked down by small interfering RNAs, and the differenti-al expression of tyrosine-phosphorylated proteins in response to the konckdown of PTPLAD1 in HCT-116 cells was identified by stable isotope labeling with amino acid in cell culture ( SILAC) , coupled with the tyrosine phosphorylation antibody im-munoprecipitation and LC-MS/MS analysis.The Ingenuity Pathway Analysis ( IPA) software was employed for bioinformat-ics analysis on the differentially-expressed proteins.RESULTS:A total of 20 differentially-expressed tyrosine-phosphoryla-ted proteins were identified by MS, including 8 markedly up-regulated and 10 evidently down-regulated proteins.IPA soft-ware suggested that these proteins were mainly associated with the disease of cancer, tissue development and function, and cell death and survival.CONCLUSION:We successfully identified PTPLAD1-regulated differentially-expressed tyrosine-phosphorylated proteins in colon cancer cell line HCT-116.Our analysis suggests that PTPLAD1-regulated proteins in colon cancer are closely correlated with colon cancer.

Adult ; Humans ; Male ; Myocarditis

Objective To investigate the value of bone morphogenetic protein-7 (BMP-7) and the kidney shear wave velocity (SWV) in the diagnosis and treatment of diabetic nephropathy.Methods 150 cases of patients with diabetic mellitus were divided into three groups according to 24h urinary albumin excretion (Ualb):normalbuminuria (NA) group,microalbuminuria (MA) group and clinical proteinuria (CA) group;50 healthy subjects were selected as the control group (NC).The levels of blood BMP-7 were detected and the virtual touch tissue quantification (VTQ) was used to detect renal SWV in four groups.Both the BMP-7 level and the renal parenchyma SWV were compared among four groups.Then correlation analysis was made between blood of BMP-7 level and renal SWV in diabetic nephropathy groups.Results Blood BMP-7 level:the lowest was CA group,the middle one was MA group,and the highest was NA group (P ＜0.05),the NC group and the NA group had no significant difference (P ＞0.05); SWV measured values:CA group was the highest,MA group was middle one and the NA group was the lowest (P ＜ 0.05),the difference between NC group and the NA group was not significant statistically (P ＞0.05).The blood levels of BMP-7 and renal SWV had a significant negative correlation (r =-0.612,P ＜0.05).Conclusions The blood BMP-7 levels downregulate with the progressing of diabetic nephropathy,but the renal SWV increases with the diabetic nephropathy deterioration,and may indirectly reflects blood BMP-7 levels.Both of them have important application values in the diagnosis or treatment of diabetic nephropathy.