目的观察6分钟步行训练对慢性阻塞性肺疾病(COPD)患者的呼吸困难和耐力的影响。方法 72例COPD患者随机分为对照组36例和训练组36例。两组常规治疗方法相同,训练组另加6分钟步行训练,并评价训练前及训练2个月后的呼吸困难指数和6分钟步行最大距离进行。结果训练2个月后在FEV1(%)、FEV1/FVC%、心率和呼吸次数/分钟上两组患者治疗前后、两组间无显著性差异(P>0.05),而训练组在呼吸困难指数总体上得到改善(P<0.05),6分钟步行距离增加(P<0.05)。结论6分钟运动训练方法是一个COPD患者简易有效的运动训练方法。

The Obstectrics and Gynecology Hospital attached to Fudan University has made great achievements in the clinical and experimental study of integrated traditional Chinese and western medicine in the past 45 years. Study on induction of ovulation with acupuncture revealed the relationship between circulating estrogen and central beta-endorphin, and suggested that the depletion of central beta-endorphin resulted in the release of GnRH and ovulation, and the main indication for acupuncture treatment was pubertal dysfunctional uterine bleeding. Acupuncture was also successfully used to treat anorexia nervosa. The successful treatment of polycystic ovary syndrome (PCOS) with Yu's Tonifying Recipe for tonifying the kidney and resolving phlegm was found to be related to the elevation of serum FSH which resulted in ovulation. Good result was obtained in hyperinsulinemia pattern of PCOS treated with Tiangui Recipe for replenishing the kidney and activating blood. In studies on patients and animal model, Tiangui Recipe was found to decrease peripheral androgen and insulin levels and lead to central regulation of proopiomelanocortin, neuropeptide Y and leptin receptor, as well as regulation of neuro-endocrine-metabolic network. Thus it can promote ovulation and reduce body weight. In the studies, a new way to classify PCOS was suggested. Gengnianchun Recipe for women with postmenopausal syndrome was found to be effective in increasing estrogen receptors (ERs) and ER mRNA in hypothalamus and peripheral organs and regulating neuro-endocrine-immune-metabolic network in patients and aging rats. Gongtai was effective for controlling menorrhagia under the mechanism of regulating endometrial prostaglandins. The researches mentioned above indicate that illness is the disorder of some key-links in the life network of the patient which may be affected by factors from the environment. And this idea is different from the ideas in traditional Chinese medicine and biomedicine. It indicates that woman in subclinical health state has already got something incontrollable in her life network, and it also gives a new impact on the treatment and prevention of women's diseases as well as the prevention and treatment of women's reproductive health.

Objective To develop and verify a fluorescence quantitative detection method for antibody against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) based on magnetic bead sorting and peptide self-assembly. Methods Magnetic beads were specifically bound to nucleocapsid(N) protein of SARS-CoV-2 by click chemistry reaction, which were detected by Western blot and BCA. Using magnetic beads labeled with N protein as coating antigen and donkey anti-human IgG labeled with alkaline phosphatase(ALP) as enzyme-labeled antibody,combined with assemblable peptide and thioflavine-T(ThT)luminescence system, a quantitative detection method for SARS-CoV-2 antibody was established, and the specificity,linear range, limit of detection(LOD),precision and durability were verified. The developed method and commercial ELISA kit were used to detect 8 serum samples with known background respectively,and the consistency between them was calculated.Results Most of SARS-CoV-2 N proteins were stably bound to magnetic beads. The developed method specifically detected positive serum samples. The concentration of SARS-CoV-2 IgG antibody standard ranged from 2. 5 to 80 ng/mL,showing a good linear relationship with fluorescence value, and the linear equation was y = 182. 11 x + 5 095. 5, R~2= 0. 99;The LOD was 2. 5 ng/mL;The coefficient of variation(CV)within batches ranged from 3. 9% to 10. 6%,the CV between batches ranged from 6. 0% to 13. 8%, and the CV of durability was within 1. 3%～6. 2%. The consistency between the results of the developed method and commercial ELISA kit was high(R~2= 0. 99,P < 0. 000 1). Conclusion The developed fluorescence quantitative detection method based on magnetic bead sorting and peptide self-assembly showed good specificity,precision and durability, which might be used for quantitative detection of SARS-CoV-2 antibody.

Endometriosis, a common and complicated disease in gynecology. is characterized by strong invasive-ness, wide implant and easy recurrence. At present, its pathogenesis is still not clear and there is no satisfactory therapy, so it has become a hot issue in gynecology research. This paper reviewed its pathogenesis and Traditional Chinese Medicine and West ern Medicine treatment.

Objective: To investigate the relationship between vocabulary understanding ability and performance of theory-of-mind tests of children and to confirm the applicability of Chinese revision of Theory of Mind Tests in China. Methods: 100 children aged from 3 to 5 years in Harbin City in China completed the Chinese revision of Theory of Mind Tests and Peabody Picture Vocabulary Test(PPVT). Results:The pass rates of 'expression recognition' tests from 3 to 5 years groups were 100%, and that of 'expression reasoning' tests were 76%,97%,97%. There were 36%,76%,93% of children passed the 'place change' false belief tests and 15%,42%,59% of children passed the 'content change' false belief tests. From 3 to 5 years groups, significant differences of PPVT IQ were found between groups divided depending on different performances of false belief tests, such as that of children passed place change or not were 104.9?16.9 vs 97.0?15.8,106.7?17.6 vs 99.7?15.9(t=2.22,2.07, P

Parasomnias induced by hypnosedatives are rare but serious side effect. Such parasomnias have not been reported with all hypnosedatives. However, frequent use of hypnosedatives, particularly nonbenzodiazepine receptor agonists is associated with parasomnias. Associated symptoms are sleep eating, sleepwalking with object manipulation, sleep conversations, sleep driving, sleep sex and sleep shopping etc. Mechanisms include high affinity for GABAA receptor, interruption of the consolidation phase of memory formation by drug, pharmacokinetic or pharmacodynamic drug-drug interaction and concomitant administration with alcohol. Managements for parasomnias induced by hypnosedatives involve stopping medication, switch to other medications or nonpharmacological treatment, lowest effective dose of NBRAs (Non-Benzodiazepine Receptor Agonists), taking into consideration drug-drug interactions, identification and treatment of underlying disease states.
Eating ; Hypnotics and Sedatives ; Memory ; Parasomnias ; Somnambulism

目的观察75岁以上老年人急性心肌梗死(acute myocardial infarction,AMI)静脉溶栓治疗的疗效。方法48例75岁以上采用尿激酶溶栓治疗的患者与同期94例<75岁的AMI患者溶栓治疗做对照。结果≥75岁组的再通率为68.7%,<75岁组为72.3%;轻度出血分别为6.3%和5.3%,重度出血均为0%;心率失常分别为41.6%和49.9%;病死率为6.3%和5.3%。两组患者12h静脉溶栓治疗再通率无显著性差异,并发症及病死率也无显著性差异(P>0.05)。结论75岁以上老人静脉溶栓治疗是安全有效的。

OBJECTIVE: To develop an HPLC method for simultaneous determination of catalpol, leonuride, adenosine, cistanoside A and acteoside in Rehmanniae Radix. METHODS: The HPLC analysis was carried out on a Grace Allitma C18 column (4.6 mm × 250 mm, 5 μm), with a linear gradient elution of acetonitrile-water at the flow rate of 1.0 mL · min-1. The detective wave length was set at 203 nm, and the column temperature was set at 25°C. RESULTS: The calibration curves were linear within the range of 12.47-997.22 μg · mL-1 for catalpol, 2.85-227.78 μg · mL-1 for leonuride, 0.36-28.75 (μg · mL-1 for adenosine, 1.30-104.17 μg · mL-1 for cistanoside A, and 1.65-131.94 μg · mL-1 for acteoside, respectively. The average recoveries for the five marker compounds were 98.8%-100.0%, r =0.9997-1.0000. CONCLUSION: This method is simple, accurate and practical for the quality control of Rehmanniae Radix. There are some differences in the contents of the five marker compounds in the samples from different producing areas. Copyright 2013 by the Chinese Pharmaceutical Association.

Objective To investigate the levels of peripheral blood plasma M-ficolin/L-ficolin and their correlation with clinical indexes in patients with rheumatoid arthritis (RA).Methods M-ficolin/L-ficolin was detected in the plasma of 61 RA patients who were grouped according to the disease activity and 18 healthy controls by enzyme-linked immunosorbent assay (ELISA).The relationships between the levels of M-ficolin/L-ficolin and laboratory parameters,disease activity were investigated in RA patients.Statistical analysis were performed by t-test,one-way analysis of variance (ANOVA),rank-sum test,correlation analysis was carried out with Pearson/ Spearman rank correlation test.Results ① M-ficolin levels in 61 RA patients were [M (QR) 39 (24) ng/ml] significantly higher than healthy controls (14±4) ng/ml (Z=-5.72,P＜0.05).L-ficolin levels in 61 RA patients were (1.7±0.6) μg/ml obviously higher than healthy controls (1.3±0.5) μg/ml (t=2.55,P=0.013).② The M-ficolin levels of 61 RA patients in low,mediate,high disease activity group were (21±5) ng/ml,[M(QR) 35(13) ng/ml),[M(QR) 50(25) ng/ml].There was significant difference among the three groups (P＜0.01).The L-ficolin levels of 61 RA patients in low、mediate,high disease activity group were (1.2±0.5) μg/ml,(1.7±0.4) μg/ml and (2.2±0.6) μg/ml.There was significant difference among the three groups (P＜0.05).(M-ficolin levels were positively correlated with disease activity score (DAS)28,erythrocyte sedimentation rate (ESR),C reactive protein (CRP),rheumatoid factor (RF),white blood cell (WBC),Note Express User Tool (NEUT),MONO,blood platelet (PLT) (r=0.830,0.692,0.725,0.667,0.388,0.397,0.338,0.476,P＜0.05),L-ficolin levels were positively correlated with DAS28,ESR,CRP,RF,WBC,NEUT,MONO,PLT (r=0.653,0.593,0.630,0.468,0.254,0.302,0.312,0.340,P＜0.05).Conclusion M-ficolin/L-ficolin levels increase in RA patients and correlate with disease activity,which suggests that the complement system may play an important role in the pathogenesis of rheumatoid arthritis.

The current situation is that the study of blood system diseases is difficult, and the students lack both the initiative and enthusiasm to learn. Clinical teachers' energy is limited because of other clini-cal, management and research roles, so the quality of teaching varies greatly. Meanwhile, teaching quality has nothing to do with personal reviews. Furthermore, the relationship between doctors and patients is in trouble in current medical environment. All of these above affect the quality of clinical practice in hematology department. Aiming at these problems, we improve the teaching quality significantly by raising the students' interests, enhancing their initiative, improving teachers' teaching level and enthusiasm, carrying out the in-ternship system, and strictly enforce the elimination system.