Objective To identify the chemical components of traditional Chinese medicine by liquid chromatography-mass spectrometry.Methods Chemical components of 86 traditional Chinese medicines were identified by two methods,observation group with LC-MS method and control group with traditional LC methods.The identification efficacy,accuracy,and identification time between two methods were compared. Results The excellent rate in observation group was 76.74% (66/86),the excellent and good rate was 90.70%(78/86),which were significantly higher than those in control group (P<0.05 ).83 kinds of traditional Chinese medicine were successfully identified in observation group,the success rate was 96.5 1%,significantly higher than control group(P<0.05).The identification tine,retention time after correction of errors in observation group were significantly less than control group(P<0.05 ).Conclusion LC-MS has high accuracy,short identification time and wide adaptation range in identification of chemical components of traditional Chinese medicine.

Child ; Early Diagnosis ; Hand, Foot and Mouth Disease ; diagnosis ; Humans

OBJECTIVE:To provide reference for the rational drug use and glycemic control for patients with type 2 diabetes. METHODS:160 patients with type 2 diabetes in 3 communities of Tianjin were divided into 2 groups based on the understanding of the diabetic knowledge,group A(86 cases)had understanding of diabetic knowledge and group B(74 cases)had no. They were carried on the questionnaire survey about the general information,medication and glycemic control. RESULTS:A total of 160 ques-tionnaires were conducted,and 160 were effective with the effective rate of 100%. The proportion of patients with highly educated (college or above)in group A was significantly higher than group B,low education(secondary or below)was significantly lower than group B(P<0.05). The proportion of patients with medicine background in group A was significantly higher than group B(P<0.05). There were no significant differences in the use ratio of different types of drugs for diabetes between group A and group B (P>0.05),however,the use ratio of self-purchase functional food in group A was 0,and group B(60.8%)was significantly high-er than group A(P<0.05). The recent self-test average blood glucose levels,the average blood glucose levels in the investigation day,the 2 h postprandial blood glucose and glycosylated hemoglobin levels in group A were significantly lower than group B(P<0.05). CONCLUSIONS:The medication and glycemic control of patients with type 2 diabetes is closely related to the understand-ing of glycemic knowledge. Therefore,propaganda and education of medication and related functional food for patients need to be strengthened to promote the rational medication.

Carbapenems ; pharmacology ; Drug Resistance, Bacterial ; Humans ; Klebsiella Infections ; drug therapy ; microbiology ; Klebsiella pneumoniae ; drug effects

Euthyroid Sick Syndromes ; blood ; etiology ; Female ; Gestational Age ; Humans ; Hypothyroidism ; blood ; etiology ; Infant, Newborn ; Infant, Premature ; Male ; Risk Factors ; Sex Factors ; Thyronines ; blood ; Triiodothyronine ; blood

Adolescent ; Adult ; Aged ; Chilblains ; therapy ; Female ; Ginger ; Humans ; Male ; Middle Aged ; Moxibustion ; Seasons ; Young Adult

Objective: To analyze the regulation of hospital accounting procedure under the new hospital accounting system. Methods: Summarize the achievement in accounting and find the existing problems by field investigation. Results: Because the content of accounting system reform is big and the requirement is high, the accounting is not in coincident. Conclusion: Since there is imperfection in accounting and unrelated accounting procedure, it is needed to repeat, clarify and unify the management, so as to standardize the hospital financial behavior and reflect the accounting information accurately.

Objective To explore the value of MRI sequence and MRA for diagnosis of moyamoya disease(MMD).Methods Retrospective analysed of 1 8 cases of moyamoya disease clinical datas,1 8 cases were used routine head MRI scan,including T1 WI, T2WI,DWI,transverse and sagittal T2WI sequence FLAIR;MRA three dimensional time of flight(3D-TOF).Results 18 cases of patients with MRA showed bilateral internal carotid artery,anterior cerebral artery distal and proximal middle cerebral artery steno-sis or occlusion of signs.Among them,1 1 cases were found with secondary cerebral infarction,3 cases of cerebral hemorrhage,7 ca-ses of centrum semiovale,corona radiata and periventricular cerebral ischemia,4 cases of cerebral atrophy,8 cases of cerebral soften-ing and gliosis.Conclusion MRI and MRA examination have important value in the diagnosis of moyamoya disease,treatment.

Objective To explore if the expression of follicle-stimulating hormone ( FSH ) and its receptor ( FSHR) exist in the rat submandibular gland tissue , which may provide a theoretic evidence for further studying on adjusting function of the FSH in submaxillary gland .Methods Twenty normal SD rats , with a mean body weight of (200 ±20)g, were adopted to collect out the submandibular gland after abdominal cavity anesthesia .The method of in situ hybridization was employed on cellular localization of FSH and FSHR .RT-PCR was used to detect if FSH and its receptor mRNA exist in the rat submandibular gland .RNA was extracted from the submandibular gland , then FSH and the core cDNA of its receptor gene were obtained with reverse transcription polymerase chain reaction and analyzed on its sequence . Results The epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats had FSH and FSHR mRNA hybridized signals , which were detected in cytoplasm with negative nuclei .Specific bands of FSH cDNA and FSHR cDNA were identical to be 193bp and 413bp respectively.Conclusion FSH and FSHR can be synthesized by the epithelial cells of serous acinus and granular convoluted tubule in submaxillary gland of rats , thus this study further proves that the submandibular gland is the target organ of FSH .

OBJECTIVE: To discuss the biosafety and biocompatibility of biomaterials. METHODS: A computer-based online search of CNKI, CBM and VIP as well as manual search were performed to collect articles about random cell control experiments and animal experiments of biological material biocompatibility published between 1990 and 2008. A total of 28 Chinese literatures were collected, and 7 were included mainly involving the cytotoxicity test methods and blood compatibility of the experimental medium, experimental grouping, experimental materials, methods of observation, experimental results, and experimental conclusion. In addition, biocompatibility of the biological material was analyzed to summarize the biocompatibility of the biological safety. RESULTS: Based on experiments of biosafety and biocompatibility of biomaterials, the cytotoxicity test and blood compatibility of various biomaterials, such as collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, are essential for the biological safety. The experimental results have shown that the biomaterials have good biocompatibility. CONCLUSION: A variety of biological materials present good biocompatibility, including collagen, chitosan, magnetic nanoparticle, metal vascular stent, sulphurated siliastic medical grade silicon rubber, polyurethane, ceramic coatings by micro-arc oxidation, based on the evaluation criteria.