Objectives:To investigate the prevalence situation and antibiotic resistance of chlamydia trachomatis (CT),ureaplasma urealyticum(UU) and mycoplasma hominis(MH) in infertile female patient,and to analyze the resistant of mycoplasma to common antibiotics. Methods:The cervical secretion samples from 2186 female infertility and 210 pregnancies women were collected. Then chlamydia trachomatis antigen was detected by immunoassay. Mycoplasma (UU and MH) were isolated and tested antimicrobial susceptibility for 12 kinds of antimicrobial drugs. Results:In the trial group 173 were CT-positive at a rate of 7.9%,and 1102 were positive for the mycoplasmae at a rate of 50.4%,of which 987 were UU infections and 115 were MH infections.The number of CT,UU and MH infections totaled 1275 cases,leading to an overall infection rate of 58.3%. The top three antibiotics for drug sensitivity in the UU,MH and UU+MH cultures were josamycin,minocycline and clarithromycin.The three antibiotics,to which the pathogens were most tolerant,included ofloxacin,norofloxacin and sparfloxacin. Conclusion:The infection rate of chlamydia and mycoplasma in infertility women is obviously higher than normal pregnanted,this shows the fact that chlamydia and mycoplasma infection of genitourinary tract may be one reason of infertility.The sensitivity of mycoplasma to common antibiotics especially to quinolones and macrolides has decreased.

Chronic lung disease is a very common complication caused by inhaled hyperoxia, mechanical ventilation and pulmonary infection in preterm infants. It shows early inflammation and late alveoli fusion with mesenchymal fibroblast proliferation. Mitogen activated protein kinase is a very important signal transduction pathway in eukaryotic cells.It plays an important role in the cell inflammation, proliferation, differentiation, survival and apoptosis, which may contributes to the chronic lung disease.

Objective:To investigate the effect of microRNA-144-3p (miRNA-144-3p) on drug resistance sensitivity of thyroid cancer cells by targeting and regulating paired box gene 8 (PAX8).Methods:Human thyroid cancer cell line FTC-133 was cultured in vitro and selected to construct the cisplatin-resistant cell stains. Reverse transcription polymerase chain reaction (RT-PCR) was used to detect the relative expression of miRNA-144-3p in FTC-133 cells and cisplatin-resistant FTC-133 cells. The cisplatin-resistant FTC-133 cells were divided into group A, group B, and group C. Group A received no treatment, group B was transfected with empty plasmids, and group C was transfected with pCDNA3.1+ miRNA-144-3p plasmids. RT-PCR was used to detect the relative expression levels of miRNA-144-3p and PAX8 in each group, and methyl thiazolyl tetrazolium (MTT) method was used to detect the half inhibitory concentration (IC 50) value of cisplatin on cells in each group, proliferation rate in each group was detected by cell counting kit-8 (CCK-8) method, and apoptosis rate in each group was detected by flow cytometry. Results:The relative expression level of miRNA-144-3p in cisplatin-resistant FTC-133 cells was significantly higher than that of FTC-133 cells [(0.93±0.24) vs (0.26±0.04), P<0.05]; The IC 50 value, proliferation rate, apoptosis rate and relative expression levels of miRNA-144-3p and PAX8 in cisplatin-resistant FTC-133 cells in group B were not significantly different from those in group A ( P>0.05). The IC 50 value, proliferation rate and relative expression of miRNA-144-3p of cisplatin resistant FTC-133 cells in group C were significantly higher than those in group B ( P<0.05), and apoptosis rate and relative expression of PAX8 were significantly lower than those in group B ( P<0.05). Conclusions:Overexpression of miRNA-144-3p may increase cisplatin resistance of thyroid cancer cells by up-regulating PAX8 gene expression, thus promoting the proliferation of thyroid cancer cells and inhibiting their apoptosis.

Objective:To investigate the effects of radix actinidiae chinensis extracts on the proliferation and apoptosis of breast cancer cells by regulating the vascular endothelial growth factor (VEGF)/phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT) signaling pathway.Methods:Human breast cancer cell line MCF7 was cultured in vitro and divided into control group, low-dose group, medium dose group and high-dose group. The low-dose, medium dose and high-dose groups were added with DMEM culture medium diluted with 1, 10 and 100 μg/ml radix actinidiae chinensis extracts respectively. The control group was added with equal dose DMEM culture medium for 48 hours. The proliferation rate (detected by methyl thiazolyl tetrazolium method), apoptosis rate (detected by flow cytometry) and the protein expression of PI3K, VEGF and phosphorylation-AKT(p-AKT) in each group were compared (detected by Western blot). Results:Compared with the control group, the proliferation rate and PI3K, VEGF, p-AKT protein expression of MCF7 cells in low dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in low dose group was significantly increased ( P<0.05); compared with low dose group, the proliferation rate and PI3K, VEGF, p-AKT protein expression of MCF7 cells in medium dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in medium dose group was significantly increased ( P<0.05). Compared with the middle dose group, the proliferation rate of MCF7 cells and the expression of PI3K, VEGF and p-AKT protein in the high dose group were significantly decreased ( P<0.05), and the apoptosis rate of MCF7 cells in the high dose group was significantly increased ( P<0.05). Conclusions:Radix actinidiae chinensis extracts may inhibit the proliferation and promote the apoptosis of breast cancer cells by inhibiting the VEGF/PI3K/AKT signaling pathway.

It was pointed out that the development of theory of TCM was restricted by the indistinct concepts.The advantages and disadvantages of the concept between traditional Chinese medicine and western medicine were analyzed.Basing on literature researches and modern science researches can solve the concept problems of the theory of TCM.

This paper,introduced the manufacture process and quality control method of Xiao- erqingfei Suppository.The suppository tallied with requirements of China Pharmacopeia (the 85th edition).Its main active components were identified with TLC.Two represen- tative components were determined:ephedrine and glycyrrhizic acid.

AIM To explore whether or not the function of insulin like growth factor (IGF I) in promoting osteopontin gene expression and protein synthesis of rat vascular smooth muscle cell (VSMC). METHODS Cultured cells were incubated with different concentration of IGF I for different hours, then RT PCR and Western blotting analysis were used to observed the effects of IGF I on osteopontin expression of rat vascular smooth muscle cells RESULTS IGF I (20 ?g?L -1 ) could obviously induce the gene expression and protein synthesis of osteopontin, RT PCR results showed that the ratio of mRNA of osteopontin and ? actin in cells incubated with IGF I (20 ?g?L -1 ) for 3, 24 and 48 h increased by 39 70%,40 44% and 54 05% respectively, as compared with that of the control ( P

OBJECTIVE: To summarize the cause and mechanic of inflammation reaction induced by medical biomaterials in the body.DATA SOURCE: A computer-based online search of Pubmed database was undertaken to identify the relevant articles published from January 2004 to December 2008 in English with the key words of "biomaterials, inflammation, mechanism".Meanwhile, with Chinese articles were retrieved from CNKI database between January 2005 and December 2008 with the key words of "biomaterials, inflammation, mechanism".DATA SELECTION: Articles concerning the cause and mechanic of inflammation reaction induced by medical biomaterials were included. Articles addressing physical and chemical features and mechanics of medical biomaterials were excluded.MAIN OUTCOME MEASREUS:①After the material implants in vivo, inflammatory cell and material adhering.②Cell factor expression.RESULTS: After initially examined 70 literatures were obtained. According to inclusion and exclusion criteria, the reasons and the mechanism related to the medical biological material in host in vivo for causing the inflammation were analyzed. Along with the tissue engineering development, various new high-polymer medicine biological materials with good biological compatibility,the biological activity and the biodegradation absorption function are emerging unceasingly. The biomedicine material was used to repair or substitute damage organization and the organ, and to cause function recovery. The infection was still the serious complication following the medical biological material was implanted. The mechanism of biomedicine material-induced infection is: complement in blood adhere the biological material surface, which causes collection and adherence of integrin-mediated leukocytes, release of cytokines and growth factors from adherent monocytes/macrophages, resulting in the occurrence of biomedicine material-related infection. To prevent the medical biological material-related inflammation, we should first embark from the material itself, and seek for some to respond the small medical material to the host inflammation.CONCLUSION: The success of material implantation depends on the medical biological material relevant inflammation. The production of biomaterial-related inflammation is action results of inflammatory cell, inflammation factor, complement as well as enzyme, oxygen free radical. Material surface microstructure, chemistry and dielectric and so on immediately influence inflammatory cells on material response.

Dentin Sensitivity ; prevention & control ; Humans