Objectives:To investigate the prevalence situation and antibiotic resistance of chlamydia trachomatis (CT),ureaplasma urealyticum(UU) and mycoplasma hominis(MH) in infertile female patient,and to analyze the resistant of mycoplasma to common antibiotics. Methods:The cervical secretion samples from 2186 female infertility and 210 pregnancies women were collected. Then chlamydia trachomatis antigen was detected by immunoassay. Mycoplasma (UU and MH) were isolated and tested antimicrobial susceptibility for 12 kinds of antimicrobial drugs. Results:In the trial group 173 were CT-positive at a rate of 7.9%,and 1102 were positive for the mycoplasmae at a rate of 50.4%,of which 987 were UU infections and 115 were MH infections.The number of CT,UU and MH infections totaled 1275 cases,leading to an overall infection rate of 58.3%. The top three antibiotics for drug sensitivity in the UU,MH and UU+MH cultures were josamycin,minocycline and clarithromycin.The three antibiotics,to which the pathogens were most tolerant,included ofloxacin,norofloxacin and sparfloxacin. Conclusion:The infection rate of chlamydia and mycoplasma in infertility women is obviously higher than normal pregnanted,this shows the fact that chlamydia and mycoplasma infection of genitourinary tract may be one reason of infertility.The sensitivity of mycoplasma to common antibiotics especially to quinolones and macrolides has decreased.

Chronic lung disease is a very common complication caused by inhaled hyperoxia, mechanical ventilation and pulmonary infection in preterm infants. It shows early inflammation and late alveoli fusion with mesenchymal fibroblast proliferation. Mitogen activated protein kinase is a very important signal transduction pathway in eukaryotic cells.It plays an important role in the cell inflammation, proliferation, differentiation, survival and apoptosis, which may contributes to the chronic lung disease.

Congenital heart disease often lost the opportunity of radical sureryfor secondary pulmonary hypertension.pulmonary hypertension is pathologicallycharacterizzed by pulmonary vascular remodeling,including the vascular smooth musclecell' s proliferation,migration and extracelluar matrix deposition,Recently,someresearchers have found that connective tissue growth factor can bind with somesurface receptors of vascular smooth muscle cells,causing some biological behaviorchanges such as proliferation,migration and extracellular matrix abnormaldeposition,and activating crresponding gene expression through this signaltransduction pathway.Pulmonary vascular remodeling may be one of the molecularpathogenesis in pulmonary hypertension.

This study was to evaluate the risk factors of nocturnal hypoglycemia in elderly patients with type 2 diabetes by continuous glucose monitoring system. Fifty-one type 2 diabetic patients aged 60 or above were enrolled and the episodes of nocturnal hypoglycemia were documented. The risk factors of nocturnal hypoglycemia were analyzed by logistic regression and the cut-off of glucose levels at bedtime for nocturnal hypoglycemia was evaluated. There were twenty-two patients with total 681 nocturnal hypoglycemic episodes. Logistic regression analysis showed that the lowest glucose level at bedtime was the risk factor of nocturnal hypoglycemia (OR=0.36, 95% CI:0.13-1.00, P<0.05), while the gender, age, diabetes duration, therapy regimen, the highest and average glucose levels at bedtime were not associated to nocturnal hypoglycemia. Receiver operating characteristic curve (ROC) showed that the bedtime glucose at≤6.2 mmol/L was the best cut-off point for predicting nocturnal hypoglycemia.

Cariostatic Agents ; therapeutic use ; Dental Caries ; prevention & control ; Fluorides ; therapeutic use ; Humans

Rational use of antibiotics can delay the emergence of antimicrobial resistance, and antimicrobial resistance surveillance provides the basis for standardizing clinical rational use of antibiotics. Therefore, antimicrobial resistance surveillance network is of great importance, and its construction can improve the rational use of antibiotics.The construction of antimicrobial resistance surveillance network should emphasize the capacity building of clinical microbiology laboratory and enhance quality control of resistance data based on the standards put forth by Clinical and Laboratory Standards Institute ( CLSI) .

Objective To explore the application value of color Doppler ultrasound in screening of fetal con-genital heart disease and its significance.Methods 4 132 cases of the expectant mothers were selected,using color Doppler ultrasonography on the line screening of five section to detect fetal cardiac,to compare with the actual clinical situation and analyze the occurrence of fetal congenital heart disease.Results Among 50 cases(1.21%) of congeni-tal heart disease,28 cases were live births, accounted for 56.00%,13 cases of induced abortion, accounted for 26.00%,9 cases died,accounted for 18.00%.48 cases were detected by ultrasound,relevance ratio was 1.16%,the accuracy rate was 96.00%,the missed diagnosis rate was 4.00%.28 cases of live births undergoing surgical correc-tion,treatment effect was good,48 cases were detected,including 10 cases of ventricular septal defect,6 cases of tetral-ogy of Fallot,6 cases of atrial septal defect,5 cases of endocardial cushion defect,4 cases of single chamber,4 cases of persistent truncus arteriosus,3 cases right ventricular dysplasia,3 cases of tricuspid valve shift malformation,2 cases of persistent left superior vena cava,2 cases left ventricular dysplasia,2 cases of aortic arch interruption,1 case of rhabdomyoma.Conclusion Using color Doppler ultrasound detection for pregnant women can effectively detect severe congenital heart disease in the fetus,stop gravid timely,which can effectively improve the level of prenatal and postnatal care that is worth clinical application.

The International Society on Thrombosis and Haemostasis has proposed to hold World Thrombosis Day on October 13 annually from 2014 to improve awareness and education of thrombosis. Thrombotic diseases are multidisciplinary disorders involving multiple systems.Thus, multidisciplinary team treatment for thrombotic diseases should be developed in China.A comprehensive study of genetic factors for thrombotic diseases is expected to achieve early diagnosis and risk prediction for this type of disease, and thus precision diagnosis and individualized treatment can be achieved.

Objective To evaluate the vascular changes of uterus and ovary in women with recurrent spontaneous abortions(RSA).Methods Fifty women were enrolled into this study: women with RSA(RSA group,30 cases) and normal women with sterility(control group,20 cases).In the mid-luteal phase of a non-pregnancy cycle,transvaginal pulsed Doppler ultrasonography of the uterine artery and ovarian artery were performed.Resistence index(RI),pulsatility index(PI),endometrial thickness,serum estradiol, progesterone were determined.Results ①The endometrial thickness in RSA group was less than that in the control gruop[((8.3)?(1.7))mm and((9.4)?(1.2))mm,respectively,P

OBJECTIVE:To explore the effect of sodium selenite intervention on the activity of NF-?B in renal tissue of rats with different stages of diabetes mellitus(DM).METHODS:SD rats were randomly divided into these groups after the diabetics model were established successfully:DM2 group(DM rats at 2 months),DM4 group(DM rats at 4 months),DM+Se2 group(Se-treated DM rats at 2 months),DM+Se4 group(Se-treated DM rats at 4 months),and set up CON2 group(normal control group at 2 months)and CON4 group(normal control group at 4 months)stimultaneously.All these groups were treated by corresponding methods after feeding different diets for 2 or 4 months,UAER was computed;Scr,BUN,Ucr and the activity of NF-?B in renal tissue were detected.RESULTS:UAER level and activity of NF-?B in DM+Se2 group were all higher than in CON2 group(P