To analyze the effect of the treatment of dual plane breast augmentation ( one part of the implantation was located behind breast parenchyma and the other part was located behind the pectoralis majior muscle) use areo-lar papillaris incision. Using the areolar papillaris incision complete dual plane breast augmentation. For some breast potsis patients the superfluous areolar papillaris skin was removed and suspension fixation was performed at the same time. Some patients were followed up for 3-months to 2-years. All surgical outcomes were satisfactory with natural breast shapes, and there were no complications such as capsular contracture, prosthesis shift or burst. U-sing the areola papillaris incision can complete dual plane breast augmentation surgery without endoscopic guid-ance;surgery is simple under directvision.

T-cell non-Hodgkin lymphoma is a group disease with unique clinical and pathological features. It is more aggressive and has lower chemotherapy sensitivity than B-cell lymphoma. In recent years, to search for effective treatment, novel agents such as gemcitabine, targeted therapy combined with chemotherapy and high-dose chemotherapy combined with hematopoietic stem cell transplantation are applied in the treatmentof T-cell non-Hodgkin lymphoma. And some progresses are achieved.

The measures taken by Library of Southern Medical University to improve the use of its electronic re-sources were described according to the major problems in use of electronic resources in domestic college and uni-versity libraries from resources assessment, resources integration and revealing, resources popularization and librarians support, with certain rational suggestions proposed for increasing the utilization ratio of electronic resources, inclu-ding popularization of resources, training of user information literacy, and improvement of users ability.

Objective　To define the rational extent of radiation portal in adult patients with early-stage Hodgkin's disease (HD).Methods　From Jan 1984 to Dec 1997,206 adult(≥15 year-old) HD patients with stage Ⅰ～Ⅱ lesions were admitted into the cancer hospital of Chimese Academy of Medical Sciences. 130 cases received radiotherapy alone (RT group). 76 cases radiotherapy combined with chemotherapy (R+C group). Involved-field (IF) was used in 7 patients (3.4%),mantle-field(MF) in 34 (16.5%) patients,subtotal nodal irradiation (STNI) in 140 (68.0%) and total nodal irradiation (TNI) in 25 (12.1%) patients. Kaplan-Meier method was used in survival analysis and log rank test for comparson.Results　①The overall 5-,10-year accumulated survival rates (ASR) were 85.1% and 73.2%. The 5-,10-year disease free survival rates (DFSR) were 68.0% and 63.6%, respectively.② The 5-year ASRs in the MF,STNI and TNI groups were 69.2%,93.3% and 94.4%,respectively (P<0.05)；the 5-year DFSRs in these respective groups were 54.2%,79.2% and 79.9% (P<0.05).③ In R+C patients ,the 5-ASRs in the IF/MF and STNI/TNI groups were 75.7% and 90.6%(P<0.05); 5-year DFSRs were 43.1% and 73.3%(P<0.05).Conclusions　The stage ⅠA patients with favouable prognostic factors can be cured by using mentle-field only,but patients in the other early-stages should be given subtotal nodal irradiatiion when radiotherapy is given alone. It is suggested that patients with unfavorable prognostic factors should receive radiotherapy combined with chemotherapy.

[Summary] From November 2013 to March 2014, 3D laparoscopic operations were carried out in 14 cases in the department of urology of our hospital .The operation went smoothly , without conversions to laparotomy .The operation time was 54-466 min, with an average of 180 min.The intraoperative blood loss was 5 -400 ml, with an average of 70 ml.No pressure sores or operation complications occurred.The medical staff participating in the operation generally felt a good 3D vision, without sense of vertigo.We deem that during urological operations which have different operation postures , it is very important to protect both laparoscopic system and operation area from pollution .Timely and properly wearing 3D glasses, application of one-eye-detection method for lens sharpness , and the switching between 2D and 3D modes can reduce and relieve visual fatigue .

Objective To compare the hepatic differentiation potential of human umbilical cord mesenchymal stem cells (UC-MSCs) with bone marrow mesenchymal stem cells (BM-MSCs).Methods UC-MSCs and BM-MSCs derived from passage 3 were induced by IMDM supplemented with 20 μg/L HGF and 20 mg/L α-FGF.The medium was changed twice a week.The concentrations of albumin and urea nitrogen from cultural medium were measured to compare the differentiation ability of the two cells.We also examined the expression of hepatic related genes by real-time RT-PCR.Results UC-MSCs manifested significandy stronger proliferation potential than BM-MSCs.Both UC-MSCs and BM-MSCs could be induced into hepatocyte-like cells.The morphology of UC-MSCs tended to be more mature than BM-MSCs and they had more cytoplasmic granules.After 4 weeks,the levels of albumin and urea nitrogen from the cultural medium of the UC-MSCs group were significantly higher than the BM-MSCs group (P ＜ 0.05).Real-time PCR showed the expressions of four liver related genes CK18,G6P,HGF and ALB in the UC-MSCs group were significantly higher than the BM-MSCs group (P ＜ 0.05).Conclusion UC-MSCs had higher hepatic differentiation potential than BM-MSCs.Thus,UC-MSCs are more suitable than BM-MSCs for tissue engineering in the treatment of end-stage liver diseases.

Objective To establish a method for simultaneous determination of 6 kinds of organophosphorus pesticides in water.Methods The liquid-liquid extraction,gas chromatography with RTX-50 capillary column and flame photometric detector were used to determine 6 kinds of organophosphorus pesticides(DDVP,dimethoate,malathion,methyl parathion,parathion,chlorpyrifos)in water.Results Chromatographic resolutions for the above 6 kinds of organophosphorus pesticides were both over 1.5.The linear range was 2.0-16.0 ?g/ml(r=0.999 2～0.999 8),the detection limits were 0.009 2～0.076 ng,RSDs were 1.5%-7.1%,the average recovery rates were 93.0%-98.7%.6 kinds of organophosphorus pesticides could be absolutely separated on RTX-50 capillary column.Conclusion This method presents an advantage in detection limit,precision and accuracy compared with that in GB13192-1991,it will meet the requirement of Sanitary Standard for Drinking Water and routine water quality analysis.

Objective To establish a HPLC method to detect the propofol concentration of plasma in children undergoing cardiopulmonary bypass. Methods RP-HPLC with fluorescence detector was used in the study. Plasma samples were precipitated by the thymol-methanol solution, and centrifugated. 20?l of supernatant fluid was injected for analysis and separated by Dikma Diamonsil C18 column. The mobile phase was composed of acetonitril:water =56:44(V/V), flo rate was 1.0ml/min. Fluorescence detective wave lengths were ?ex= 276nm, ?em=312nm. HPLC machine was Agilent1100 series. Main column was Diamonsil C18(150?4.6nm,5um),guard column was Phenomenex Security(4mm?3mm). Results There was a good linear relationship within the range of 0.02～8.0mg/L(r=0.9993, n =9),the limit of detection was 5?g/L,average recovery was 96.99%,intra RSD(relative standard deviation )was between0.810% and 5.73%,inter RSD was between 0.932% and 7.337%. Conclusions The method is sensitive, simple, rapid, good linear relationship and precise. The experiment can be used for determination of propofol concentration in child undergoing cardiopulmonary bypass.

Objective: To observe the role of nutritional support in treatment of chylous leakage after thyroid carcinoma operation.Methods: The parenteral nutrition combined with somatostatin and re-operation was used in treatment of 2 cases of chylous leakage after thyroid carcinoma operation.Fluid volume from neck drainage catheter and plasma albumin concentration were determined in 2 cases.Results: Fluid volume from neck drainage catheter rapidly reduced in 2 cases after using somatostatin and parenteral nutrition.1case was cured after conservative treatment.1 case was cured after re-operation combined with nutrition support.Conclusion: By using nutrition support,fluid volume from neck drainage catheter can be obviously reduced and serum albumin or nutritional state improved in chylous leakage patients after thyroid carcinoma operation.

0 05),RA was lower(P