ObjectiveTo observe the clinical curative effect of applying large dose of raw rhubarb and mirabilite for treatment of patients with acute pancreatitis (AP).Methods A prospective study was conducted; 63 hospitalized patients with AP accompanied by bowel repletion heat bind syndrome identified by Traditional Chinese Medicine (TCM) differentiation method admitted to Sichuan Province Forestry Center Hospital from January 2012 to June 2015 were enrolled, and they were divided into an observation group (32 cases) and a control group (31 cases) according to random number table method. The two groups received western conventional treatment, additionally the control group applied Dachengqi decoction and Qingyi decoction with addition and subtraction of herbs according to the patient's individual situation (ingredients: radix bupleuri 15 g, radix paeoniae alba 15 g, the raw rhubarb (after) 15 g, radix scutellariae 9 g, rhizoma coptidis 9 g, radix aucklandiae 10 g, corydalis 15 g, mirabilite 9 g (a blunt), aurantii fructus immaurus 15 g, magnolia officinalis 15 g), while in the observation group, on the basis of treatment in the control group, additionally, a large dose of raw rhubarb 30 g decocted at last in the decoction and mirabilite 30 g in a large amount of drinking water were taken, 360 mL decoction was prepared for each one of the two groups, 120 mL per 8 hours was injected via a gastric tube, and after the injection of drug, the tube was closed for 1 hour. No adverse reactions were seen, and after the recovery of intestinal function, the method of taking decoction was changed to oral administration. When the daily defecation was above 3 times, the dose should be reduced, and more than five times, the drug should be withdrawn. The abdominal pain, abdominal distension relief time, blood amylase recovery to normal time, peritoneal irritation disappearance time, ventilation and defecation time, length of stay in hospital, cost of hospitalization, incidence of adverse reaction (abdominal pain), complications disappearance time and prognosis were observed in two groups.Results The abdominal pain and abdominal distension relief time (hours: 30.2±12.1 vs. 41.1±15.1), blood amylase recovery to normal time (hours:121±38 vs. 180±56), peritoneal irritation disappearance time (hours: 31.1±25.2 vs. 43.6±21.2), intestinal ventilation and defecation time (hours: 31.5±25.5 vs. 43.3±26.2), length of stay in hospital (days: 20.5±3.2 vs. 25.6±3.9), complications disappearance time (days:7.6±2.9 vs. 11.5±3.1) were significantly shortened in observation group than those in control group (allP < 0.05); the cost of hospitalization was lower in observation group than that in control group (yuan: 6 077 vs.7 589,P < 0.05). the incidence of adverse reactions (diarrhea) was higher in the observation group than that in the control group [18.75% (6/32) vs. 16.13% (5/31)], but there was no statistical significant difference between the two groups (P > 0.05).Conclusion The therapeutic effect of integrated conventional western medicine and dachengqi decoction and qingyi decoction with addition of a large dose of rhubarb and mirabilite for treatment of patients with acute pancreatitis is significantly better than that of using conventional dose of the above treatment without the addition.

Objective: To study the protective effect of coenzyme Q_10 on red blood cell membrane and its immunity in patient during extracorporeal circulation. Method: Twenty patients under extracorporeal circulation about 30 minutes were randomly divided into control group and coenzyme Q_10 group (n=10). Coenzyme Q_10 2mg/kg was added to the priming fluid in coenzyme Q_10 group. Plasma levels of malondialdehyde (MDA), free hemoglobin (FHb), immune adhe sion ability and immune compound of red blood cell membrane were measured before extracorporeal circulation, 15 and 30 minutes after beginning of cardiopulmonary bypass, and on first day morning after operation. Result; All different periods after beginning of cardiopulmonary bypass in coenzyme Q_10, group, MDA and FHb levels were lower and im mune adhesion ability was higher than those of control group. Conclusion: Coenzyme Q_10 may protect red blood cell membrane and its immunity of patient during extracorporeal circulation.

Objective To investigate the expression of H19 imprinted genes in the villus tissues of women with spontaneous abortion.Methods Reverse transcription-polymerse chain reaction method(RT-PCR) was employed to detect H19 imprinted genes allele-specific expression levels of the villus tissues extracted from 45 cases of spontaneous abortion and 30 cases of normal pregnancy.Results Nineteen cases of 21 heterozygous cases in spontaneous abortion samples showed billelic expression of H19(19/21),whereas no billelic expression was found in the normal pregnancy samples(0/13)(P

Objective:To investigate cerebral angiography Features of patients with ischemic cerebrovascular disease.Methods:One hundred and twenty seven patients with ischemic cerebrovascular disease were selected in our hospital from March 2013 to April 2015, statistical analysis of their cerebral angiography differences at different ages, different types of ischemic cerebrovascular disease.Results: One hundred and twenty seven patients were found 97 patients(76.4%) had cerebral artery occlusion or stenosis, simple intracranial stenosis 40 cases, 31 cases of simple extracranial stenosis, 26 intracranial and extracranial stenosis cases; on the view of age, young patients with simple intracranial stenosis (69.2%), while the proportion of elderly patients in intracranial and extracranial stenosis was 40.5%, significantly higher than the proportion of old and middle-aged patients; patients with ischemic cerebrovascular disease at different types of stenosis and stenosis had no significant difference.Conclusion: Patients with ischemic cerebrovascular disease in different age have different distribution and different stenosis, especially in the largest proportion of young patients with occlusion, while parts of the brain in patients with vascular stenosis and stenosis and ischemic cerebrovascular disease the type was no significant association.

There has been no detailed reiport on the changes of the activity of serum glycylproline dipeptidyl aminopeptidase (GPDA) in patients with pancreatic career.Tn this paper,the serum level of GPDA was measured in 166 cases with various malignant and benign gastrointestinal diseases and 60 normal controls.It was found that the serum GPDA activity in patients with pancreatic cancer was 101,02?75.84u/L and significantly higher than that in the patients with pancreatitis and in the normal controls.There was a statistically significant difference of the GPDA activity value between the patients with malignant and benign pancreatic diseases.The elevation of GPDA activity was more marked in those patients with cancers in the head of the pancreas.In addition,the elevation of GPDA activity was dependent on the differentiation of cancer cells but not on the size of cancers.Significantly higher level of GPDA was also found in patients with hepatic carcinoma and carcinoma of ampulla of vater but significantly lower level in patients with gastric cancer.It is believed that the determination of serum GPDA activity might be helpful for the diagnosis of pancreatic cancer.

Objective To investigate the effect of resveratrol on the proliferation and invasion of human pancreatic cancer PANC-1 cells. Methods Five groups including blank control group, 0. 1% dimethylsulfoxide (DMSO) group and resveratrol groups (50, 100, 200 μmol/L) were established. The proliferation of PANC-1 cells was detected by MTT assay. The apoptosis and cell cycle change were analyzed by flow cytometry. The invasive ability of PANC-1 cells was observed with a Transwell cell culture chamber. The expressions of Bax, Bcl-2,matrix metalloproteinases 2 (MMP-2) and 9 (MMP-9) of the PANC-1 cells were assayed by real-time quantitative PCR and Western blot. All data were analyzed using the analysis of variance. Results ( 1 ) The inhibition rate of resveratrol on the proliferation of PANC-1 cells was 0 in the blank control group, 3.25% ±0.42% in the 0. 1% DMSO group, 13.23% ± 1.68% in the 50 μmol/L of resveratrol group, 42.25% ± 3.20% in the 100 μmol/L of resveratrol group, and 56.94% ±5.31% in the 200 μmol/L of resveratrol group. There was a significant difference in the inhibition rate among the five groups (F=460. 10, P<0.05). (2) The apoptosis rate was 0.05% ±0.03% in the blank control group, 3.39% ± 1.77% in the 0. 1% DMSO group, 6.92% ± 1.85% in the 50 μmol/L of resveratrol group, 19.05% ± 2.01% in the 100 μmol/L of resveratrol group, and 27. 17% ±6.43% in the 200 μmol/L of resveratrol group. There was a significant difference in the apoptosis rate among the five groups (F = 38.84, P < 0.05). (3) There was no significant effect of 0. 1% DMSO on the cell cycle of PANC-1 cells. The number of PANC-1 cells in the G0/G1 and S phase was increased. (4) The average number of invading PANC-1 cells was 61 ± 13 in the blank control group, 54 ± 13 in the 0. 1% DMSO group, 48 ± 15 in the 50 μmol/L of resveratrol group, 23 ±6 in the 100 μ mol/L of resveratrol group and 18 ±7 in the 200 μmol/L of resveratrol group. There was a significant difference in the number of invading PANC-1 cells among the five groups (F = 69.08, P < 0.05 ). (5) There were up-regulated mRNA and protein expressions of Bax and down-regulated mRNA and protein expressions of Bcl-2, and the expressions of MMP-2 and MMP-9 of the PANC-1 cells were inhibited in the resveratrol groups. The changes of the protein expressions of Bax, Bcl-2, MMP-2, MMP-9 were consistent with the changes of the mRNA expressions of the four indexes. Conclusion Resveratrol can significantly inhibit the proliferation and invasion, as well as induce apoptosis of PANC-1 cells in vitro.

Objective To investigate the curative effect of complete ablation and partial ablation of parathyroid for secondary hyperparathyroidism (SHPT) by using ultrasound-guided radiofrequency ablation (RFA) or microwave ablation (MWA). Methods A total of 26 patients with hyperparathyroidism secondary to uremia, who were admitted to authors’ hospital during the period from October 2012 to September 2013 to receive full ablation (n=11) or partial ablation (n=15) of parathyroid with MWA or RFA and were followed up for at least 9 months after the treatment, were included in this study. The clinical data were retrospectively analyzed. The therapeutic effect and the influence of MWA and RFA on the serum calcium levels were compared between the two ablation methods. Results Analysis of parathyroid hormone (PTH) levels indicated that in full ablation group the postoperative PTH level was gradually decreased , when compared with the preoperative level, until it became stable and maintained the normal level, and no recurrence was observed;in partial ablation group the postoperative PTH level was decreased first with a subsequent rebound about nine months after the treatment, and this PTH level was higher than the PTH level of full ablation group as well as higher than the normal level (P<0.05). On the 15 patients of partial ablation group, recurrence was seen in 5. Analysis of serum calcium levels showed that the postoperative serum calcium level was decreased in both groups, but in partial ablation group the serum calcium levels determined at 3 and 9 months after the treatment were higher than those in full ablation group (P<0.05). In 11 patients of full ablation group, hypocalcemia occurred in 4. Conclusion In treating SHPT, full ablation of parathyroid is not likely to have postoperative relapse, but it might carry the risk of hypocalcemia; while partial ablation of parathyroid is likely to have postoperative relapse, but the serum calcium level can be well controlled. Considering from the view that controlling of the serum calcium level is clinically very important, partial ablation of parathyroid may be more suitable for clinical purpose.

Thirty male weanling WAK strain rats were divided rondomly into 6 groups. All of the diets were pure synthetics according to AOAC. The 1st, 2nd and 3rd group were given diets containing 6, 15 and 24 ppm of zinc respectively, and 10% protein for all three groups, on the other hand, the 4th, 5th and 6th group were given diets containing 5% 10% and 20% of protein respectively, and 15 ppm of zinc.After feeding in stainless steel cage singly for 50 days rats were given intubately solution of 65Zn containing 1.5 ?Ci per 100g of body weight. Then the faeces and urine of rats were collected on 5 consecutive days, and the zinc absorption and retention of all of the tested rats were determined with method of 65Zn as tracer. The results indicated that. (1) The zinc absorption and retention of rats fed on the lowest zinc level were increased significantly. (2) The zinc absorption and retention of rats fed the lowest protein level were decreased significantly, and were improved clearly by increasing protein intake level. (3) The urine excretion of zinc is very low and can not be used as an index in zinc metabolism research. The methodology for determining zinc absorption and retention in organism with method of 65Zn and total zinc is discussed.

Objective To study the effect of varicocele(VC) on the expressions of hypoxia-inducible factor-1?(HIF-1?) and apoptosis-associated gene Bax in germ cells of adolescent rats,and investigate the mechanism of the infertility resulting from varicocele. Methods The varicocele model was established by partial ligation of the left renal vein in adolescent male Wistar rats. A total of 25 rats were randomly divided into 2 groups,namely varicocele group(n=15) and sham operation group (SOG,n=10). All the animals were killed 30 d after operation. The left testis was collected and the expression of HIF-1? and Bax was detected by immunohistochemical method. Results The expression of HIF-1? and Bax in left testis of varicocele group was significantly higher than that of sham operation group(P

Objective To explore the proliferative and apoptotic effect of 3 different siRNAs targeting survivin in human bladder cancer cell line EJ28. Methods Three siRNAs targeting survivin and 1 fluorescence-labeled siRNA as a negative control were chemically synthesized. Transfetion efficiency was observed under fluorescence microscope after transfecting the fluorescence-labeled siRNAs. EJ28 cells were divided into 6 groups: siRNA164 group, siRNA167 group, siRNA389 group, and negative control group, lipofectin group and cell control group. The proliferation of EJ28 cell was detected by MTT assay at 24, 48 and 72 h after the transfection. Apoptotic rate was detected by Annexin V-FITC-FCM assay at 48 h.Results All the sequence-specific siRNAs targeting survivin efficiently suppressed the proliferation of EJ28 cells. siRNA 164 had the most powerful effect because of its highest inhibitory rate of (41.32?2.54)% and the highest apoptotic rate of (13.20?0.25)% at 48 h after transfection. Conclusion RNAi targeting survivin has a potential value in the gene therapy of bladder cancer.