Adult male rats weighing 250-300 g were used to study ACPase and ATPase in the liver following 60Co radiation on the whole body for a single dose 2000 rad(95.7-99r/min). The irradiated group and the control group were sacrificed at intervals of 1, 2, 3 days after irradiation. The left liver lobe was removed, fro/en in dryice-isopentane, and then cut in a cryostat. ACPase and ATPase activities were revealed by histochemical and cytochemical methods respectively.In the control group, ACPase activity was found to be mainly localized in the vicinity of bile canaliculi, and generally higher in the periportal region than in the centrilobular region. The distribution of ATPase reaction products was much like that of ACPase.On the 1st day after irradiation, a slight decrease was found in ACPase and ATPase activities, while on the 2nd and 3rd days in both of them was found a marked increase especially in the periportal region. These results were in agreement with cytochemical observations.The significance of these results and related problems were discussed.

Autoimmune diseases are characterized by the presence of various autoantibodies and may cause injuries to multiple organs,with kidney as the most common and important organ involved.Autoantibodies are of great importance in the diagnosis,treatment and prognosis of autoimmune renal diseases.Lupus nephritis,anti-neutrophil cytoplasmic antibody (ANCA) associated vasculitis and antiglomerular basement membrane (GBM) disease are the most common autoimmune renal diseases.Anti-C1q antibody,ANCA and anti-GBM antibody play important roles in those diseases,respectively.Appropriate and steady detecting methods are crucial to clinicians,and the results should also be interpreted with great cautions.

Application research on human amniotic membrane has been carried out for nearly a hundred years and people found that there were more than dozens of kinds bioactive substances in the amniotic membrane. It has been proved that the amniotic membrane has a lot of functions, such as anti-inflammatory, anti-bacterial, anti-virus, anti-angiogenic and promoting cell apoptosis, and soon. As effective treatments, amniotic membrane has been used for adjunctive therapy of burns, trauma, ophthalmic damage, dermatopathya. Recent advances of amniotic membrane and amniotic membrane-derived cells research have led to enormous progress in skin tissue engineering, vascular tis- sue engineering, biological scaffold material, and biological sustained-release materials. Amniotic membrane and amniotic membrane derived cells have a significant advantage and unique charm in medical field. Therefore, they have higher research value and broad prospects in the applications.
Amnion ; Biomedical Research ; trends ; Humans ; Tissue Engineering ; Treatment Outcome

Objective To observe the protective effect of Danggui Buxue Decoction on 60 Co-γray irradiated mice. Methods The experimental mice were randomly divided into 5 groups ( the normal control group , model group , Danggui Buxue Decoction of low , middle and high dose groups ) .The mouse except the normal control group were irradiated by 60Co-γray and established the radiation-damage model.Peripheral blood neutrophil count (NEUT), the number of bone marrow nucleated cells ( BMC ) , the activity of serum superoxide dismutase ( SOD ) and the serum levels of malondialdehyde ( MDA) were measured .Results Radiation injury in mice of NEUT , BMC and SOD were significantly decreased (P <0.01), MDA were increased (P <0.01).Danggui Buxue Decoction can increase the levels of NEUT , BMC and SOD in mice after radiation injury , decrease the content of MDA .And protective effect of high dose group was better (P <0.01).Conclusions Danggui Buxue Decoction has protective effect on radiation injury in mice .And there is a correlation between the protection effect and dose .

Retrovirus (RV), a major pathogen in acute gastroenteritis in children that may lead to severe disease and heavy burdens on national economy. There is no speciifc drug treatment for the gastroenteritis caused by RV, and vaccine has been identiifed as the most effective intervention to control the associated disease burden. The RV vaccine showed differential effects on different RV infection. In China, epidemiology of RV is more complex hence demands more epidemiologic studies. In this article, we will review the epidemiology of RV in China and further discuss the importance of generalized RV vaccine.

To search the statistical approaches for evaluating and dealing with the center effects in multi-center clinical trial. Breslow-Day test was used for evaluating the differences of the virtual rating among the centers whose responses were binary, Cochran-Mantel-Haenszel test for dealing with the center effects whose responses were binary or ordinal, and logistic regression for evaluating and dealing with the center effects simultaneously. The results showed that Breslow-Day test is invalid in evaluating the center effects whose response is ordinal, and Cochran-Mantel- Haenszel test can not be used for dealing with the effects of other covariates meanwhile.

0.05).Both SBP and DBP were declined significantly in both two groups(P0.05).Heart rate decreased from (81?9)b/min to (68?7)b/min in bisorpolol group.But there wasn't this effect in contrast group.It was superior to that of contrast group significantly too(P

AIM:To study the ingredients of sediments from Xiexin Decoction(XXD)(Radix et Rhizoma Rhei; Radix scutellariae and Rhizoma coptidis) with the method of LC/MS2. METHODS:A C 18 column was used,with acetonitrile-acetate buffer as mobile phase,and the online detection wavelength was 270 nm. RESULTS:The ingredients of sediments from XXD were nearly the same as its supernatant fluid,There were the similarities between the solubilities of sediments from XXD in artificial gastric juice and in artificial intestinal fluid. CONCLUSION:In clinical trials we should pay much attention to this fact that sediments from XXD and its supernatant fluid have similarly importance.

Objective To explore a novel surgical treatment for chronic suppurative otitis media (CSOM) and evaluate its treatment effect.Methods All 97 patients with chronic suppurative otitis media were chosen to be treated using this new surgical method.The skin of the external auditory canal was maintained intact.Open radical mastoidectomy was used to complete clean-up lesions ; the fascia of pedicled temporalis myofascia (PTM) was used to repair the tympanic membrane.The pedicled temporalis fascia,pedicled postauricular periosteal flap and intact skin of the external auditory canal were used in reconstruction of the posterior wall of external auditory canal.Pure tone audiometry (PTA) was performed before and after surgery,recording the air conduction and bone conduction thresholds at 0.5 kHz,1.0 kHz,2.0 kHz,4.0 kHz.The average of the patient's air and bone conduction hearing thresholds was recorded at the 4 frequencies.External auditory canal gauze was removed 3 weeks after surgery.All subjects were followed up for over 2 years.Comparison of hearing thresholds (PTA) was made ① Before and 4 weeks after surgery.② Before and 2 years after surgery.Hearing function comparison include air conduction (AC),bone conduction (BC) and air-bone gap (ABG) analysis.SPSS 16.0 was used in statistical analysis.Pre-and postoperated AC,BC and ABG were compared using T-test.P ＜ 0.05 was considered statistically significant.Results The healing rate of post-operated tympanic membrane was 95.88％ (93/97).Ninty-six ears had 2-year follow-up,and 1 patient was lost in follow-up.There were 2 patients presented with eardrum perforation during the follow-up,and the 2-year healing rate of tympanic membrane perforation was also 93.85％ (92/97).In 96 ears with 2-year followed-up,the average of pre-AC was (52.10 ±3.96) dB,the average of post-AC was (35.67 ±2.52) dB; the average of preABG was (36.6 ± 5.2) dB,and the average of post-ABG (± SD) was (12.14 ± 6.20) dB.Statistical analysis showed significant difference between preoperative and postoperative AC or ABG values (P ＜ 0.05).Conclusion The present surgical procedure broke through the existing conventional mastoidectomy of making a surgical incision in the posterior wall of the external auditory canal.This procedure cleared the lesion completely and preserved the physiological function of the external auditory canal.The acoustic systems and state of gasification to the mastoid tymnpanum were reconstructed,rehabilitated and maintained.The healing rate of hearing and tympanic membrane perforation was improved.