Nine healthy adult cats were used in the investigation.Lumbar incision wasmade in one side and the coeliac ganglion was exposed under Nembutal anesthesia.One or two mg of HRP(Sigma Type Ⅵ)dissolved in 7 to 18 microlitres of distilled water was slowly injected into the ganglion via two or three insertions.Theleft coeliac ganglion was injected in four cats,the right in the other four,and theganglia of both sides were injected in the rest one.After a postoperative survivalof 4～5 days,the animals were perfused through the heart with 2% paraformalde-hyde and 1.25% glutaraldehyde in 0.1 M phosphate buffer(pH 7.4).Frozen serialsections(48 micra thick)of the postfixed spinal ganglia(C_3～L_7)were made andprocessed for the blue reaction using benzidine according to the Mesulam's eighthprocedure.The sections were counterstained with neutral red.HRP granules in the labelled cells in the spinal ganglia were discrete and easilyidentified under bright-field microscope,although the number of the granules variedfrom cell to cell.After the injection into the coeliac ganglia in ten sides,a totalnumber of 12,381 labelled cells were observed in the spinal ganglia.The greatermajority of them(more than 99%)were found ipsilateral to the side of injection.The labelled cells were found in the spinal ganglia from T_2 to L_2,but most of thesecells(78.1%)were distributed in the T_5 to T_9 segments.Furthermore,56.26% ofthe total number of them was situated at the level of T_(6～8),with the highest densityof the labelled cells in the seventh thoracic ganglion,constituting 22.19% of thetotal number.Although the number of labelled cells observed varied from case tocase,their percentage in the constitution of each ganglion in every case was similarto the general plan given above.There is no conspicuous difference between theleft and right sides in respect to the segmental distribution of labelled cells.Thelabelled cells were mainly of medium and small sizes.The larger cells,whichaccount for only 2.78% of all labelled cells,were very rare,and most of themwere found in the T_(5～9)spinal ganglia.The results of our investigation show that the visceral sensory fibers passingthrough the coeliac ganglion in one side come mostly from the ipsilateral spinalganglia T_5 to T_9.And about half of these fibers originate from T_6 to T_8 ganglia.Considering the fact that the greater majority of labelled cell bodies are of mediumor small size,it has been inferred that the fibers that arise from them are finemyelinated or unmyelinated and a part of them,at least,mediate the visceral pain.The labelled larger cells might be related to Pacinian corpuscles in the mesentery.It is difficult for the superior mesenteric ganglion to be separated from the coeliacganglion in cat;therefore,the labelled sensory neurons described above include,at least,a certain number of cell bodies of afferent fibers which traverse thesuperior mesenteric ganglion.

Twelve adult cats were used in our experiment.Under sodium pentobarbitalanesthesia one to two mg of HRP(Sigma Type Ⅵ),dissolved in 7 to 15 microlitresof distilled water,was slowly injected into the ganglion at two or three sites.Theleft coeliac ganglion was injected in seven cats,the right in other four and in oneboth sides were injected.After postoperative survival of 2～5 days,the animalswere perfused through the left ventricle with a mixture of 2% paraformaldehydeand 1.25% glutaraldehyde in 0.1 M phosphate buffer(pH 7.4).Serial frozen crosssections of the spinal cord were cut at 48 micra after postfixation and all sectionsof even numbers were selected and processed with the blue reaction according toMesulam's eighth procedure(1976)and counterstained with neutral red.The labelled cells were found in ipsilateral cord of the injected ganglion,andwere distributed in a transverse zone which may be divided into four nuclei:1.thenucleus intermediolateralis pars principalis(IL_p)2.the nucleus intermediolateralispars funicularis(IL_f)3.the nucleus intercalatus(IC)and 4.the nucleus interca-latus pars paraependymalis(IC_(pe)).The total number of the labelled cells in eachcase varies from 1,179 to 92.The segmental range of the labelled cells extendedfrom the T_1 to the L_1 segments in the longest and from the T_4 to the T_(10)or fromthe T_3 to T_9 segments in the shortest.The data were treated with the statisticalweighted method,and the result shows:about 69.93%?0.57 of the total labelledcells are located in the spinal segments from T_5 to T_8,and about 21.61%?0.52 inthe T_6 segment.The percentage of labelled cells in the four nuclei given above isas follows:IL_p:77%,IL_f:12%,IC:9% and IC_(pe):2%.Furthermore the majorityof labelled cells in IC and IC_(pe)were found in T_(5～8)segments,the greatest densityof them was in the T_6 segment of spinal cord.We found no obvious difference between the left and right in the pattern of thedistribution and the segmental arrangment of the labelled sympathetic preganglionicneurons.

OBJECTIVE: To investigate the effect of aerobic exercise on immune factor IL-4, TNF-alpha in patients with allergic rhinitis, which provide a theoretical basis for the treatment of allergic rhinitis. METHOD: Thirty-two allergic rhinitis patients were selected and divided into a control group (14 people) and the experimental group (18 people). During the treatment, both groups receive conventional symptomatic treatment. Meanwhile, aerobic exercise therapy is applied to the experimental group for 40 - 60 minutes a day, 3 - 4 days a week. Serum IL-4, TNF-alpha and allergic rhinitis symptoms graded scoring were tested before and after 6 month therapy. RESULT: After six months of exercise intervention, the 11-4, and TNF-alpha levels of experimental group were significantly lower than the level of control group. The symptoms score is also significantly decreased in experimental group. CONCLUSION Aerobic exercise had good therapeutic effect in patients with allergic rhinitis. The mechanism may be that aerobic exercise can reduce the level of IL-4 and TNF-alpha, which can relieve infiltration and activation of inflammatory cells.
Adolescent ; Adult ; Exercise ; Exercise Therapy ; Female ; Humans ; Interleukin-4 ; blood ; Male ; Rhinitis, Allergic ; Rhinitis, Allergic, Perennial ; blood ; therapy ; Tumor Necrosis Factor-alpha ; blood ; Young Adult

OBJECTIVE: To establish a RP-HPLC method for the content determination of gentiopicroside in Xiaofeng pills.METHODS: The chromatographic column was DiamonsiLTM C18(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of acetonitrile-water(11 ∶ 89) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm.RESULTS: The linear range of gentiopicroside were 0.205 6～2.056 0 ?g (r=0.999 9) with an average recovery of 96.18%(RSD=1.55%,n=6).CONCLUSION: The method is simple,rapid and accurate for the quality control of Xiaofeng pills.

Objective: To observe and summarize the routine maintenance situation, and research relevant countermeasure and provide the optimal solution based on the problems in maintenance of medical equipment. Methods: To adopt SWOT method to analyze the problem of equipment maintenance, and make effective countermeasures by using relevant statistical methods. Results: The SWOT method that was applied in maintenance work has improved accuracy of maintenance work for equipment, and increased operation rate of equipment and serviceability rate, at the same time, the satisfied degree of medical staffs and patients has been increased. Conclusion: Through SWOT method to analyze the situation of maintenance for equipment, the work key points can be effectively improved, and the blindness and dead ends of equipment maintenance management can be found. Therefore, this method can reduce the labour intensity of maintenance staffs, increase work efficiency, and provide essential guarantee for medical work of hospital.

The myelodysplastic syndrome (MDS),which are characterized by the presence of ineffective hematopoetic stem cells.Research on pathogenesis and related epigetics has made significant progress in recent years,more and more abnormal cytogenetics and gene mutations are used for prognosis and treatment options for MDS.In the 56th American Society of Hematology (ASH) annual meeting,lots of gene mutations were introduced.

Objective: To explore the relationship among advancod aged hypertension,unstable angina pectoris (UAP)and high homocysteine (Hcy)level.Methods:A total of 208 advanced aged patients with hypertension and angina pectoris,who were diagnosed and treated in our department of cardiology,were divided into H-type hyper-tension group (n=102)and general hypertension group (n=106).Each group was further divided into UAP sub-group and stable angina pectoris (SAP)subgroup.Levels of Hcy,blood glucose,total cholesterol (TC),high densi-ty lipoprotein cholesterol (HDL-C)and triglyceride (TG)were measured;Gensini scoring system was used to score extent of coronary artery lesion in these patients.Results:Compared with general hypertension group,there were significant rise in incidence rate of UAP (25.47% vs.46.08%)and Gensini score [(30.2±15.6)scores vs.(46.1 ±18.7)scores]in H-type hypertension group,P <0.05;in H-type hypertension group,Hcy level in UAP subgroup was significantly higher than that of SAP subgroup [(22.8±7.2)μmol/L vs.(13.1±2.7)μmol/L],and percent-age of paitents with high Hcy level in UAP group was significantly higher than that of SAP group,P <0.05 all;in general hypertension group,compared with SAP group,there were significant rise in levels of Hcy [(7.1 ± 1.5)μmol/L vs.(9.2±2.8)μmol/L]in UAP group,P <0.05 ;Logistic regression analysis indicated that for advanced aged UAP patients,Hcy is an independent risk factor of UAP (OR=1.9,95%CI:1.1~3.0,P =0.03).Conclu-sion:UAP incidence rate of H-type hypertension patients significantly rises than that of general hypertension pa-tients,Hcy is an independent risk factor of UAP.

We here introduced in detail one special malfunction of Aucson 128xp colorful Ultrasound Diagnostic Equipment as a reference for the same trade or occupation.

The causes of "Plateau Phenomenon"in College English learning are analyzed on the basis of the investigation and the study of the Learning Curve.Some solutions to cope with the stagnation are also suggested.It's worth attention that Learning Strategy can be a vital factor to overcome the"Plateau Phenomenon."

Construction of security system plays an important role in the management of archive in research institute.With years' practice and exploration,we summarize our experiences in building up the security system of our institute here.This article involves two aspects of archive work,one is the conscious awareness of archive security and security system building.Suggestion on security cultural construction,digital file safety and data backup are also given.