The sunflower protein concentrate studied in this paper is a new developed food protein resource in Heilongjiang Province. Up to now the studied report on this product has not been seen in China. Using casein as reference protein and soy protein isolate and gluten from wheat flour as comparable substances we studied the content of major nutrients and the composition of essential amino acid in the sunflower protein concentrate by chemical analysis method, and also studied protein efficiency ratio (PER) and other indicators related to nitrogen metabolism in the animal experiment by the recommended method of AOAC. Some common harmful substances in the samples were examined quantitatively. The results showed that the protein content (as anhydrate basic), the PER, apparent digestibility, the true digestibility, the biological value, and NPU of the sunflower protein concentrate were 62.43%, 1.96, 96.83%, 98.51%, 96.81% and 95.35% respectively. The essential amino acid composition was the same as analogous products prepared in other countries. The blood indicators and pathological observation of tested rats were normal. The content of several harmful substances in the product was allowable.All of these results indicated that the nutritional quality of the sunflower protein concentrate is about the same with some similar porducts prepared in other countries. We believe that these products are safe to consumers in the aspect of food hygiene.

Child ; Diabetes Mellitus, Type 2 ; drug therapy ; metabolism ; physiopathology ; Glucagon-Like Peptide 1 ; metabolism ; therapeutic use ; Humans ; Incretins ; therapeutic use ; Treatment Outcome

Adolescent ; Aspirin ; therapeutic use ; Child, Preschool ; Diospyros ; adverse effects ; Female ; Fruit ; adverse effects ; Gastrointestinal Diseases ; drug therapy ; Humans ; Lithiasis ; drug therapy ; Male ; Treatment Outcome

Objective:To explore the effect of intensified electrocardiogram (ECG) identification by using spectrogram for the monitoring and nursing ability of nurses who worked in department of cardiology.Methods: Monitoring and nursing capacity of 120 nurses of cardiology who had been trained on the intensified ECG identification by using spectrogram were researched by retrospective analysis. The 120 nurses were divided into control group (60 cases) and intervention group (60 cases) as the random number table. The nurses of control group only received routine training of ECG identification, while the nurses of intervention group received the training of using spectrogram for ECG identification on basic routine training of control group. A series of indicators, including the scores of ECG comprehensive application ability, identification ability of abnormal electrocardiogram, and the number of the identified abnormal malignance ECG and successfully treated patients, between the two groups were compared and analyzed.Results: After the training, the scores of basic theory, abilities of description and recording, and analysis ability in field operation of the intervention group were significantly higher than those of the control group, respectively (t=11.896,t=8.596,t=8.283,P<0.05). The identified ability including room speed, atrial flutter, ventricular flutter and other abnormal ECG of intervention group were significantly better than these of control group, respectively (x2=18.836,x2=17.698,x2=16.773,P<0.05). Besides, the discovery rate of abnormal malignance ECG (73.3%) and the successful rate of treatment (100.0%)of intervention group were significantly higher than that (31.7% and 81.7%) of control group, respectively (x2=19.184,x2=10.484,P<0.05). Conclusion:The training of intensified ECG identification by using spectrogram for nurse of cardiology can effectively enhance the capacities of identification for ECG and comprehensive application for nurses, and can faster confirm the situation of patient, and can contribute to achieve precision diagnosis for doctors.

Cerebral Infarction ; drug therapy ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Humans ; Medicine, Chinese Traditional ; Neurology ; Parkinson Disease ; drug therapy ; Peripheral Nervous System Diseases ; drug therapy ; Phytotherapy

Grasses infected by endophytes have an extraordinary impact on the ecology and economy of pasture and turf. In this review, we presented recent research progress on origin and evolution, biological and ecological impact, and perspective of the association in order to make full use of the widely distributed endophyte resources.

Aims To construct the N-terminal Strep-tagged ( NS-tagged) fusion protein expression vector, and to apply the vector to express NS-tagged fusion proteins of Chlamydia RNA polymerase subunit. Meth-ods By using PCR method, NS fusion protein tag and a new multiple cloning sites (MCS) were inserted into pET21c-DH plasmid by primers to replace the original T7 protein tag and MCS. The newly introduced Not I cutting site was chosen for self-ligation of PCR prod-uct. Then, the cyclized PCR product was transformed into DH-5α competent cells. The positive clones were selected by PCR and sequencing. To get NS-tagged fu-sion proteins of chlamydial RNA polymerase subunits, the α, β and β′ subunits were inserted between BamH I and Sal I cutting sites of the newly constructed ex-pression vector. Then, the NS-α, NS-β and NS-β′ ex-pression vectors were transformed into Arctic Express expression cells. The fusion protein expression statuses of transformed cells were identified by Commassie blue staining and Western blot. Results The NS-tagged fusion protein expression vector pET21c-NS-MCS was successfully constructed, and NS-α, NS-β and NS-β′fusion proteins were obtained by using this newly con-structed expression vector. Conclusions In this pro-ject, we constructed an NS-tagged fusion protein ex-pression vector and applied it to express NS-α, NS-βand NS-β′ fusion proteins. Our study can lay a solid foundation for the study of transcriptional regulation of Chlamydia genes.

Objective To evaluate the clinical effectiveness of percutaneous transhepatic gallbladder drainage introduced by bedside ordinary-probe-guidance with free-hand technique in severe cholecystitis. Methods 85 cases treated with the operation were observed and analyzed. Results Infectious bile was successfully drained in all cases without serious complications. 83 cases received satisfactory outcomes. 2 elderly patients died from MODS secondary severe infection. Conclusion Bedside ordinary-probe-guided free-hand percutaneous transhepatic gallbladder drainage is a safe and convenient treatment with minimal invasion for severe cholecystitis, which can be widely used in clinic.

Objective To study the incidence of malignant tumors in renal allografts and explore the mechanism of increased incidence.Methods A retrospective study was performed on 1 120 renal allografts under at least 0.5 year immunosuppression from 1978～2000. Results 47 cases of malignant tumors were found in 1 120 cases undergoing renal transplantation with the incidence being 4.2?%. 40 cases had intact medical history and 34 cases were demonstrated pathologically. Most patients accepted operations, additional therapies including chemical or radiological and immunological therapies. 25 cases survived and the longest survived time was 4.5 years. Conclusions The incidence of malignant tumors in renal allografts was higher than normal persons. Besides the outcome of immunosuppression, the effects of transplantation itself, such as oncogene chimera, transition of carcinogenic virus and so on, can not be neglected.

Objective To study the effect of active ingredients(saikosaponin) of bupleurum chinensis D C.on GABA of hippocampus of chronic kindling induced by pentylenetetrazol(PTZ) in rats.Methods Forty-eight healthy Sprague-Dawley rats were randomly divided into six groups with eight in each group: normal control(Group A), normal saline(NS) group(Group B),sodium valproate(VPA) group(Group C),group of large-dose saikosaponin(Group D),group of medium-dose saikosaponin(Group E),and group of small-dose saikosaponin(Group F).Except Group A to be controlled,the other groups were given different treatments when chronically kindled by PTZ.The process of the test lasted for 4 weeks.After 4 weeks' administration the brain tissues were sampled,sliced and stained by enzyme immunohistochemistry(EIH).The results were analyzed according to the positive cell population and gray value.Results The positive cell population of CA1,CA2 and DG in group B was significantly different from that in the other groups(P