Fracture healing consists of four stages: hematoma and inflammation, granulation tissue formation, membranous and endochondral ossification, and remodeling. During these stages, the pathological changes are continuous and overlapping and numerous inflammatory factors and growth factors play a central role in the tissue regeneration and ossification. The fracture will eventually reach primary bone healing or callus healing depending on the stability of fracture site after treatment. The delayed union and nonunion can be assessed according to the treatment time, clinical examination, radiology and biomechanical testing. It is important to understand the differences between hypertropic and atrophic nonunions and the reasons that lead to nonunion. At last, methods to promote fracture healing are summarized and classified into four groups: biological, systemic, mechanical and biophysical ones.

Acute compartment syndrome (ACS) is still an emergent situation in traumatic orthopaedics. Its pathophysiologic mechanism and treatment principles have already been recognized and established widely. The main factors which affect the prognosis are early diagnosis and timing of surgical treatment. This review analyzes the methods of clinical and instrumental diagnosis,rectifies some misunderstandings in clinical evaluation and makes suggestions on appropriate monitoring.

Objective To observe the efficacy of treatment of cirrhosis ascites with integrated traditional Chinese medicine and western medicine. Methods All patients were randomly divided into a treatment group (27 cases) and a control group (25 cases). Patients in the treatment group were treated with integrated traditional Chinese and western medicine, while patients in the control group were treated with western conventional therapy. Resulta The total effective rate in the treatment group was 96.3%, showing significant difference (P＜0. 05) contrasting 72% in the control group. Conclusion It is effective that treating cirrhosis ascites with integrated traditional Chinese and western medicine.

Objective To observe the effect of interleukin-1β(IL-1β) on expression of Matrix Metalloproteinases 13 (MMP-13) in rat chondrocytes and its regulation of miR-27b. Methods Chondrocytes were extracted from 7 Wistar male rats. Expression of MMP-13 were examined by Western blot at 0 h, 24 h, 48 h after IL-1βstimulation. Differential miRNAs expression profiles were examined by miRNAs microarray. The most obviously down-regulated miRNAs were confirmed by quantitative Real-time PCR. Targeted regulation relationship between miR-27b and MMP-13 was set up by Luciferase re?porter gene experiments. Results Expression of MMP-13 in rat chondrocytes was increased at a timely dependent manner upon IL-1βstimulation(P<0.05);Microarray revealed 36 miRNAs whose expression changed, among which 6(miR-27b, miR-31, miR-26a, miR-26b, miR-23, miR-204)were especially obvious. Real-time PCR confirmed that miR-27b was the one whose expression level were most down-regulated. Transient co-transfection of miR-27b mimics with luciferase expres?sion plasmids resulted in significant repression of luciferase activity in rat chondrocytes (P < 0.05). Conclusion IL-1βstimulation result in down-regulation of miR-27b and up-regulation of MMP-13 expression. MiR-27b and MMP-13 show targeted regulation relationship.

Fulminant myocarditis (FM)is a clinical accidant and emergency,and often endenger the lives of patients.Myocardium damage by virus infection and immune-mediated cellular damage are important for the development of FM.This paper discussed the role of the immune system in the FM,involving cellular immunity,humoral immunity and cytokine.Also this paper discussed the treatment of FM about the immunol-oregulation and immunosuppression.

Objective To evaluate the effect of therapeutic communication on perioperative anxiety of cancer patients by using the Meta-analysis.Methods The randomized controlled studies of therapeutic communication for perioperative anxiety of cancer patients were gathered from Pubmed,Embase,Cochrane Library,China Biology Medicine Disc (CBM),China Scientific Journal Database by VIP (CSJD),China National Knowledge Infrastructure (CNKI) and Wangfang Data,while other relative researches were also searched by hand.According to inclusion and exclusion criteria articles were evaluated by two reviewers independently.The studies were assessed according to the Juni assessment.RevMan 5.2 was used for statistical analysis.Results Seven studies with 506 patients were included,of which therapeutic intervention group contained 256 patients,the control group included 250 patients.Meta-analysis showed that compared with the control group,therapeutic communication system could reduce perioperative anxiety of cancer patients [MD=-8.00,95％CI (-8.91--7.10),P ＜0.01],improve positive perioperative coping of patients [MD=5.50,95％CI(4.05-6.95),P ＜ 0.01],reduce negative perioperative coping of patients [MD=-7.62,95％CI (-9.13--6.11),P ＜ 0.01],increase the satisfaction degree of patients [MD=0.55,95％CI (0.33-0.77),P ＜0.01],the differenccs were statistically significant.Conclusions Therapeutic communication can contribute to relieve perioperative anxiety of cancer patients,optimize their coping styles and improve the satisfaction degree of patients with nursing work.

Objective To investigate heat shock protein 70 (HSP70) and nuclear factor-?appa B(NF-?B)expression in the lung after mechanical ventilation with PEEP(positive end expiratory pressure)in a rabbit model of ventilator-induced lung injury (VILI). Methods Thirty healthy adult white rabbits were randomly divided into three groups. After anesthesia and tracheostomy were installed, rabbits received ventilation with different tidal volumes for 4 hrs. In PEEP group, rabbits received ventilation withVT=40ml/kg, PEEP=3cmH_2O; group, rabbits received ventilation with VT=40ml/kg, PEEP=0cmH_2O; and NVC group, with normal ventilation with VT=10ml/kg. The different modalities of ventilation were maintained for 4 hours. After 4 hours, rabbits were killed by exsanguination. Lung histopathology was examined and compared among three groups. Expression of HSP70 and NF-?B were determined by using Western-blot technique. Results There were marked pathological changes in the lung tissue in group ZEEP, but pathological changes were relatively milder in group PEEP. After injurious ventilation for 4 hours, there was a significantly higher expression of HSP70 and NF-?B in group ZEEP, while that of NF-?B was negatively correlated with HSP70 levels. There was a significant lower expression of NF-?B in the lung of group PEEP. Conclusion We postulate that HSP70 expression protects the lung against ventilator-induced lung injury by down-regulating expression of NF-?B in the lung. Mechanical ventilation with PEEP can protect the lung against ventilator-induced lung injury through down-regulation of NF-?B in the lung.

Objective To explore the change of plasma orexin-A levels in patients with chronic obstructive pulmonary disease(COPD).Methods Lung function test and blood gas analysis were performed in 15 obese COPD patients,20 COPD patients without obesity,20 obese controls and 20 normal healthy adults(control group).In both obese COPD group and obese group,the body mass index(BMI)was higher than 25 and there was no significant difference in BMI.The plasma was deproteinized by chromatography,and the plasma orexin-A levels were determined by radioimmunoassay.Results The plasma orexin-A levels in the obese COPD group[(8.82?1.90)ng/L]and the non-obese COPD group[(8.69?1.84)ng/L]were significantly higher than those in the obese group[(7.18?1.45)ng/L(P 0.05).Plasma orexin-A levels in patients with COPD correlated negatively with partial pressure of oxygen(PaO_2)(r = -0.527 ,P

This article presents a new digital haemoglobin meter.This device adopts HICN method recommended by WHO.Its unique designing of automate proofread zero circuit and automatic controlling in determination provids high accuracy and stability,handy and reliable,It can be widely used in the hospital chemical ex- amination laboratory,commue hospital,health station and related laboratory.

Objective To study the expression patterns of neuropeptide Y(NPY)and vasoactive intestine peptide(VIP)during the bone development,and its possible relationship with angioge nesis and osteoblast differ-entiation.Methods The bones of low extremity of SD embry o and new -born rats were taken,fixed in formalin and packed by paraffin wax.The developmental stages of bone were determi ned by HE stain The representative specimen were picked out and within t he same layer the sections were taken immunohistochemical stain respective to anti-NPY,VIP,vasoepithelial grow th factor(VEGF)and alkaline phosphatatase(AP).The location and intensity of stain were compared by microscopi c observation.Results During the earliest stage of osteoge nesis,there was no sign of angiogenesis while osteob last began to differentiate and it parallels the intensity of ossificati on activity.At the first ossification center,the l ocation and pattern of NPY expressio n are similar to those of VEGF.Conclusions Due to VIP expression at the earliest stage of osteogenesis,the bone tissue may have the independent nerve control before angiogenesis.The expression similarity of timing and lo cation between NPY and VEGF suggests there is certain relationship between NPY-energetic nerve and angiogenesis.