Objective To investigate the application of heat-clearing and detoxifying Liangxue prescription decocting-free granules in puerperal infection and its clinical curative effect.Methods 97 cases patients with puerperal infection were chosen in the hospital from Dec.2012 to Dec.2014, according to the digital method, which were randomly divided into experimental group (n=49) and control group (n=48).The two groups were given metronidazole injection 250 mL/d, and 0.2 g mesylate levofloxacin added into 5% glucose injection 250 mL, intravenous drip, twice per day.On the basis of above therapy, experimental group were treated with heat-clearing and detoxifying Liangxue prescription decocting-free granules, orally, twice per day, one bag each time.Both of two groups were treated for 2 weeks as one course.Clinical symptom score, hemodynamic indexes, inflammatory factor, blood biochemical indexes and efficacy were observed and compared between two groups.Results Total effective rate of experimental group was 95.92%, which was significantly higher than 75.00% of control group ( U =3.58, P<0.01 ) .After treatment, the cell count of white blood cell ( WBC) and neutrophilic granulocyte ( GRAN ) , values of middle cell percentage (%MON ) , neutrophilic granulocyte ratio (%GRAN ) , and lymphocyte percentage (%LYM) in both of two groups significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the serum IL-6 and TNF-αlevels in two groups decreased compared with before treatment ( P<0.05 ) , and the above indexes in experimental group were lower than those in control group ( P<0.05 ) .After treatment, the hemodynamic indexes including cell aggregation index, whole blood viscosity ( high, medium and low shear ) and plasma viscosity significantly improved compared with before treatment than control group ( P<0.05 ) , and the above indexes in experimental group significantly improved compared with control group (P<0.05).After treatment, the score of hypogastralgia, chills and fever, uterine body tender and a funicular tenderness of attachment area, lochia score and total score significantly decreased compared with before treatment (P<0.05), and the above indexes in experimental group significantly were lower than control group ( P<0.05 ) .Conclusion The heat-clearing and detoxifying Liangxue prescription decocting-free granules can significantly improve the inflammatory symptoms in treatment of puerperal infection, and its mechanism of action may improve microcirculation, reduce inflammatory reaction.The clinical efficacy of therapy is obviously better than western medicine treatment alone, which can be popularized in clinical application.

Objective To analyze the hereditary background of common species from Rhodiola L. in Yunnan Province. Methods Fifty-nine samples of six populations in three natural medicinal species from Rhodiola L. were studied by RAPD method. Results The percentage of polymorphic bands (PPB) of three populations from R. crenulata were 37.97%, 46.15%, and 39.45% respectively; the Nei's gene diversities (h) were 0.110 3, 0.143 7, and 0.137 1, respectively; the Shannon's information indes (I) were 0.173 6, 0.222 1, and 0.206 7, respectively; at the special level, the PPB was 70.47%, I was (0.258 3); the genetic differentiation (G_(st)) was 0.263 0. The PPB of two populations from R. fastigiata were 43.67% and 44.42%, I were 0.215 3 and 0.217 4, respectively; at the special level, the PPB was 65.51%, G_(st) and I were 0.313 2 and 0.313 9, respectively; the PPB of R. yunnanensis was 36.97%, I was 0.188 1. Conclusion RAPD molecular marker could be used to molecular authentication and genetic diversity analysis of medicinal species from Rhodiola L.

No abstract available.

No abstract available.
Humans ; Hypogonadism* ; Male*

No abstract available.
Growth Hormone* ; Receptors, Somatotropin*

[ABSTRACT]OBJECTIVEThe influnence of observation on the antisense cytokeratin 13 (CK13) gene in nasopharyngeal carcinoma HNE1 cell transplantation tumor radiation sensitivity.METHODSHNE1 cell lines can be divided into control group: the control group (HNE1 cell) and lentivirus (transfection slow virus empty carrier) group and experimental group: HNE1-anti-CK13a (transfection antisense CK13a slow virus) and HNE1-anti-CK13b (transfection antisense slow virus CK13b) four groups, set up a corresponding animal model, after radiotherapy by flow cytometry, immunohistochemistry, PCR, Tunel method and Westernblotting detection.RESULTSThe cell cyclede tection of plasmid transfection slow virus group compared with control group after radiotherapy G2/M phase of the block were significantly prolonged; Immunohistochemical results showed emigration tumor CK13 expression decreased in the experimental group; Tunel method to detect apoptosis necrosis rate found that the experimental group,apoptosis rate significantly decreased; Western blotting detection caspase3 apoptosis markers. PCR to detect CDC25mRNA level decreased obviously.CONCLUSIONAntisense CK13 gene by regulating the cell cycle and apoptosis can reduce HNE1 transplantation tumor radiotherapy of nasopharyngeal carcinoma (NPC) sensitivity, and with the caspase 3 apoptotic pathways and CDC25 signaling pathways.

Enolase is one kind of important glycolytic enzymes which widely exists in most organisms. A number of recent studies confirm that this enzyme has the functions of activating the plasminogen involving in the processes of infection and mi-gration of parasites reducing the immune function of the host as well as preventing parasites from the immune attack of the host. This paper reviews the current research advances in the parasite enolase and explores its potential for diagnosis drug develop-ment and vaccine target of parasitic diseases.

Objective To develop and validate a qual it y of life(QOL) scale for the Chinese patients with uterocervical cancer (QOL-UC C). Methods Based on the internationally accepted de finition of QOL and the clinical characteristics of uterocervical cancer as wel l as the existing QOL scales, we designed the QOL-UCC. Then 113 patients were r ecruited and the reliability, validity and feasibility of the scale were tested. Results It was shown that the QOL-UCC had good reli ability, validity and feasibility. Conclusion QOL-U CC is a reliable and valid instrument for evaluation of the QOL in Chinese pa tients with uterocervical cancer.

Objective To discuss the application valueof the multi-slice spiral CT indiagnosing breast cancer. Methods The images of scanning, enhancing and reconstruction were analyzed in 71 cases of breast cancer proved by clinical pathology. Results All the 71cases were the type of mass. The shapes of the tumor in 57 cases were irregular, burrs can be seen in 48 cases , the skin in 8 cases changed, the nipples in 4 cases sunk, the tissue around the tumor was invaded in 12 cases; The CT value of the tumor rised greatly after enhancement, theranges of value was form 18 to 83 HU. Axilla lumph nodes were detected in 32 cases, Wecan find the lumph node in mediastinum and lung metasis; The tumor and tissue around it can be shown well in multi-orientations through images reconstruction. Conclusions The multi-slice spiral CT has the evident application value in pualitative diagnosing breast cancer.

Macrofungi are rich in natural bioactive compounds so they have much medicinal value.Their immunomodulating and anticancer activity particularly attracts more attention.Based on the polysaccharides molecular structure and their relation to these activities,this paper discusses their mechanism of the immune responses and introduces the important instances of these polysaccharides along with their immunomodulating and/or anticancer activity isolated from putative medicinal mushrooms.