This study was aimed to establish an HPLC method for simultaneous determination of the content of echinacoside, acteoside and isoacteoside in Total Cistanchis glycosides Capsules. Waters C18 column (4.6 mm ×150 mm, 5 μm) was used with the mobile phase of methanol-0.1% formic acid at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 330 nm. The column temperature was maintained at 30℃ . The results showed that the linear ranges of echinacoside, acteoside and isoacteoside were in the range of 27.792-277.92 μg·mL-1 (r = 0.9996,n = 6), 2.4184-24.184 μg·mL-1 (r = 0.9996, n = 6), 5.106-51.06 μg·mL-1 (r = 0.9998, n = 6). The average recoveries of three components were in accordance with the determination requirement. It was concluded that the method was simple and accurate, which can be used in the content determination of echinacoside, acteoside and isoacteoside in Total Cistanchis glycosides Capsules.

OBJECTIVETo investigate the levels of prostatic specific antigen (PSA) in patients with chronic prostatitis.

METHODSForty-five patients with diagnosed inflammatory chronic pelvic pain syndrome (NIH category III A prostatitis) were inquired about the history symptoms and signs of prostatitis, and underwent digital rectal examination of the prostate as well as analysis of expressed prostatic secretions (EPS). Bacterial infection was also analyzed by pre- and post- massage test (PPMT), and PSA in the blood was detected. Thirty healthy males without inflammation in EPS were selected as controls.

RESULTSIn 45 cases of the patients with NIH category III A prostatitis, the PSA level in the blood was 2.41 +/- 0.64 microg/L, and that in the control group was 0.93 +/- 0.52 microg/L, with significant difference (P < 0.05). And among the 45 patients there were 6 (13.3%) whose PSA levels were over 4.0 microg/L, but there was only 1 in the 30 control males (3.3%). In III A prostatitis, the PSA level was elevated with the increase of inflammation in EPS, but with no significant difference.

CONCLUSIONSIn the diagnosis of prostate diseases, it should be taken into account that chronic non-bacterial prostatitis might elevate the level of PSA to a certain extent.

Adult ; Aged ; Case-Control Studies ; Chronic Disease ; Humans ; Male ; Middle Aged ; Pelvic Pain ; blood ; diagnosis ; Prostate-Specific Antigen ; blood ; Prostatitis ; blood ; diagnosis

Humans ; Cardiac Resynchronization Therapy ; Cardiac Pacing, Artificial ; Heart Ventricles ; Heart Failure/therapy* ; Treatment Outcome ; Electrocardiography ; Ventricular Function, Left