1.Labeling of CDTPA-dianhydride-coupled CD45 monoclonal antibody with yttrium-90.
Yun-bi FU ; Gui-ping LI ; Fan-yi MENG
Journal of Southern Medical University 2006;26(8):1230-1232
OBJECTIVETo explore the methods for labeling CDTPA-coupled CD45 monoclonal antibody (mAb) with yttrium-90 ((90)Y) for potential acute myeloid therapy.
METHODSCD45 mAb was labeled with (90)Y by CDTPA and the labeling rate, radiochemical purity, final specific activity, and immunological activity of the mAb were detected.
RESULTSWith the optimal molar ratio of CDTPA/Ab at 20:1, the labeling rate was 95%, radiochemical purity 99.8%, and final specific activity 1.9 mCi/mg. This conjugate was stable in vitro with comparable immunological activity in comparison with unlabeled CD45 mAb.
CONCLUSION(90)Y-CDTPA-CD45 mAb possesses good properties as an ideal targetting therapeutic agent for acute leukemia.
Anhydrides ; chemistry ; Antibodies, Monoclonal ; chemistry ; immunology ; Humans ; Immunoconjugates ; chemistry ; immunology ; Isotope Labeling ; methods ; Leukocyte Common Antigens ; immunology ; Pentetic Acid ; chemistry ; Radiopharmaceuticals ; chemical synthesis ; chemistry ; immunology ; Yttrium Radioisotopes ; chemistry
2.Gastrectomy for the treatment of refractory gastric ulceration after radioembolization with 90Y microspheres.
Sun Young YIM ; Jin Dong KIM ; Jin Yong JUNG ; Chang Ha KIM ; Yeon Seok SEO ; Hyung Joon YIM ; Soon Ho UM ; Ho Sang RYU ; Yun Hwan KIM ; Chong Suk KIM ; Eun SHIN
Clinical and Molecular Hepatology 2014;20(3):300-305
Transcatheter arterial radioembolization (TARE) with Yttrium-90 (90Y)-labeled microspheres has an emerging role in treatment of patients with unresectable hepatocellular carcinoma. Although complication of TARE can be minimized by aggressive pre-evaluation angiography and preventive coiling of aberrant vessels, radioembolization-induced gastroduodenal ulcer can be irreversible and can be life-threatening. Treatment of radioembolization-induced gastric ulcer is challenging because there is a few reported cases and no consensus for management. We report a case of severe gastric ulceration with bleeding that eventually required surgery due to aberrant deposition of microspheres after TARE.
Aged
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Carcinoma, Hepatocellular/*diagnosis/radiotherapy
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Embolization, Therapeutic/*adverse effects
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Gastrectomy
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Gastrointestinal Hemorrhage/etiology
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Gastroscopy
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Humans
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Liver Neoplasms/*diagnosis/radiotherapy
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Magnetic Resonance Imaging
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Male
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*Microspheres
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Radiopharmaceuticals/therapeutic use
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Stomach/pathology
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Stomach Ulcer/*etiology/surgery
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Yttrium Radioisotopes/chemistry