It is a new approach to investigate the ultrastructure, function and metabolism of each segment of uriniferous tubule using dual environment culture chambers (DECC). In the present study the DECC culture of proximal tubule cells was undertaken with millcell-HA culture insert in the mixed medium of Ham's F12 and Dulbecco's modified Eagle's medium.A monolayer of proximal tubule cells was obtained in the medium mixed by constant bubling of oxygen at 3 weeks after seeding. Transmission electron microscopy revealed the cuboidal cells with microvilli, abundant mitochondria, vacuoles, lysosomes, and irregular plasma membrane infoldings. Also, the monolayer exhibited active metabolism from the release of ~(14)C-I-carbon dioxide and absorption ability of ~(14)C-I-glucose across the cultured monolayer. In addition, the culture procedure has been introduced in detail.

Wistar rats -were given orally a single dose of nitroquine acetate ( 1 mg/kg) 24 hours after an intravenous inoculation of the sporoizoites of Plasmodium yoelii yoelii strain BY 265. Liver biopsies were taken and some animals were killed at regular intervals to observe the exoerythrocytic forms(EEF). Blood smears of some animals were made to observe the parasitemia. The results were , as follows.1. The density of the EEF in the livers of the treated animals was low but the number of degenerated EEF was high 48 hours after inoculation. At this time, the EEF was smaller in size with less number of nucleus, but with irregular nuclear masses. The EEF at 54, 65 and 72 hours were characterized by un- even size of the nuclei, which were disproportionate to the size of the parasites, and by much more irregular masses of nucleus. Some degenerated EEF infiltrated by WBC could be found. There was no matured tissue schizont within 72 hours after sporozoile inoculation.2. Only 1 out of the 8 treated animals became patent of parasitemia of low level with a prolonged prepatent period.The results indicate that nitroquine exerts a causal prophylactic effect on rodent malaria and interferes with the development of EEF by suppressing the ouclear division

Objective To evaluate the relationship between Helicobacter pylori (Hp) infection and pathohistological presentation of gastric and deuodenal mucosa.Methods The gastritis biopsies were taken through endoscopy from each gastric body,antrum and duodenum in 60 patients with gastritis and 22 patients with duodenal ulcer respectively for the examination of histology.Result The positive rate of Hp was nearly 100% in active chronic gastritis which was significantly higher than that of active duodenitis.The total detecting rate of Hp infection and incidence rate of active inflammation in antrum were obviously higher than that of gastric body and duodenal bulb.The detecting rate of Hp infection of duodenal bulb in 22 cases of duodenum ulcer was 54% which was significantly lower than that of antrum.Conclusion The Hp infection is a very important pathogenic factor of active inflammation in gastric mucosa on the basis of which the severe inflammation of mucosa and ulcer development.Therefore the early elimination of Hp is very important.

Objective To investigate the pathological features and clinical outcomes of the arthroscopic treatment for calcifying tendinitis of the supraspinatus. Methods From March 2009 to October 2010,34 patients with calcifying tendinitis of the supraspinatus were treated with shoulder arthroscopy. Based on size of calcifying deposits, they were divided into three groups: small (＜10 mm), middle (≥ 10 mm, ≤20mm), and large (＞20 mm). All patients underwent arthroscopic calcium excision and subacromial decompression, and acromioplasty of shoulder was performed in 8 cases, rotator cuff neoplasty in 10. The preoperative and postoperative clinical data such as Constant's score, Visual Analog Scale (VAS) pain score and radiographs were adopted for evaluation. Besides, histological and scanning electronic microscopic examinations of calcium deposits were also carried out. Results All patients were follwed up for average 11.5 months.The patients were regularly examined at a time point of 1 month preoperatively, 2 days preoperatively and of the latest follow-up. The average Constant score was 36.1 ±6.9, 55.6±12.4 and 89.7±2.7 respectively; the average VAS pain score was 8.2±0.8,7.03±0.7,1.7±0.3 respectively. Both scoring systems revealed a significant difference before and after surgery. However, the clinical outcomes of the calcified deposit (small, middle, and large) showed on significant difference among the 3 groups. Collagen fibers were not degenerated around the calcified deposit, and no apoptotic or dead cells could be found, therefore, it is not a pathological calcification. Conclusion Recognition of calcifying tendinitis and good timing of surgery are keys to good clinical results. Shoulder arthroscopy is a safe, effective and minimally invasive method for the treatment of calcifying tendinitis of the supraspinatus. The patients with various size of the calcium deposit treated with shoulder arthroscopy can lead to an excellent clinical outcome postoperatively.

Objective To investigate the influence of Buyanghuanwu (BYHW) Decoction on cerebral infarct volume and pathological changes after cerebral ischemia and reperfusion. Methods The model of focal cerebral ischemia and reperfusion was made by thread embolism method. Healthy male SD rats were divided randomly into sham-operated group, model group and BYHW Decoction group. Infarct volume was detected by triphenyltetrazolium chloride (TTC) staining and pathological changes were observed microscopically in HE stained sections. Results In BYHW Decoction group, infarct volume was significantly smaller than that in model group (P

In the present experiments, the effect of hypertonic NaCl infusion on the renal ultrastructure in rabbits with hypovolemia was examined by electron microscopy. Rabbits were anasthetized with pentobarbital sodium and submitted to control bleeding and held at a mean arterial pressure of 40 mm Hg for 45 minutes. They were then given 6 ml/kg i. v. of 1) isotonic NaCl (300 mosm/L; 2) hypertonic NaCl (2400mosm/L). Only animals receiving hypertonic NaCl had immediate and permanent resto ration of mean arterial pressure and nearly intact ultrastructure of nephron. However, the animals with isotonic NaCl had an ischemic changes of nephron ultrastructure and unrestored mean arterial pressure.

BACKGROUND:Endovascular embolization materials have been produced and used clinicaly, but new problems constantly occur in the clinical application and new demands are also developed for embolic materials. OBJECTIVE:To retrospectively analyze the advantages and disadvantages of various types of embolic materials and to investigate the relationship between application fields and selection of embolic materials, thereby providing a reference for clinical treatment. METHODS:CNKI and PubMed databases were retrieved for relevant literature, and then embolic materials were systemicaly reviewed based on relevant clinical application and basic research literature in the folowing aspects: embolism mechanisms, clinical applications, advantages and disadvantages of various embolic materials. In this review, the existing problems in the current study were pointed out, and the development direction of relevant research and clinical application were also prospected. RESULTS AND CONCLUSION:Embolic materials should have biodegradability and biocompatibility. Current embolic materials include absorbable gelatin sponge, Polyvinylalcohol embolic agent, micro-coils and liquid embolic materials. Gelatin embolic material has no antigenicity and good histocompatibility, as wel as has excelent compressibility and water re-expansibility. Polyvinylalcohol particles are a polymer material that is insoluble in water, has high expansion coefficient, mechanical embolization, non-alergenic reaction, no acute systemic toxicity, no intracutaneous irritation, and can be used safely and effectively. Adhesive liquid embolic material has no vascular toxicity and has been widely used. Endovascular embolization materials have been produced in succession, and meanwhile, new problems in clinical applications have been found and new demands for embolic materials have been put forward continuously. Thus, a variety of embolic materials have their own advantages and disadvantages, and none of embolic materials can be applied to al diseases. To select an appropriate embolic material is very important for safe and effective treatment.

Objective:To explore the effects of class career psychological guidance(CCPG)on the career planning abilities of college students.Methods:By random sampling and control study methods,one class of college students were regarded as the experimental class of subjects,the other class was regarded as the control.The experimental class received 3 months of group training of CCPG,while the control class did not.They were assessed with the effect assessment scale of CCPG and the experimental class was retested 3.5 months later.Results:The overall level of career planning abilities had been improved in the experimental class after 3 months of CCPG,such as in the career planning confidence and the ability to solve problems relating to career,not in the control class. Most of the improved factors in the experimental class were still good after 3.5 months.Conclusion:The class career psychological guidance has a positive role in promoting the career planning abilities of college students.

Objective To investigate the protective effects of astragaloside Ⅳ on blood-brain barrier(BBB) and influence on expression of ZO-1 protein after cerebral ischemia and reperfusion in rats.Methods 72 SD rats were randomly divided into:sham operation group,model group and two astragaloside Ⅳ therapy groups [group A(10 mg/kg,group B(20 mg/kg)].Method of Evans blue leakage and water content in the brain tissue were used to investigate the permeability of the BBB,and method of immunohistochemistry combined with image analysis was used to measure the expression of ZO-1 protein in microvessels.Results Compared with those in the model group,Evans blue leakage and water content were less and the number of ZO-1 possitive vessels was increased in the two astragaloside Ⅳ therapy groups.There were statistical differences between therapy groups and model group(all P

Objective To explore the mediating effects of college students＇ online social support on the relationship between online self-disclosure and internet altruistic behavior.Methods Using cluster random sampling,the self-disclosure questionnaire for Chinese undergraduates with their internet friend,the online social support scale of adolescence and the internet altruistic behavior scale of undergraduates were applied to 351 college students.Results The score of college students＇ online self-disclosure,online social support and internet altruistic behavior were respectively(2.22±0.45),(2.93±0.59) and (2.04±0.46).The college students＇ online self-disclosure,online social support and internet altruistic behavior were positively correlated with each other (P＜0.01).College students＇ online self-disclosure not only predicted internet altruistic behavior directly,but also predicted internet altruistic behavior indirectly through online social support.The direct effects and indirect effects were 0.200 and 0.265.The ratio of the mediating effect on the total effect was 57％.The fit index of mediation model was x2/df=2.87,RMSEA =0.07,CFI =0.94,TLI =0.93,GFI=0.92,IFI=0.94,NFI=0.91.Conclusion The college students＇ online self-disclosure not only affects the interuet altruistic behavior directly,but also can take the same effect on online social support indirectly.