Objective　To study the effect of HAI and/or PVI after radical resection of hepatocellular carcinoma (HCC) for preventing recurrence.Methods　From Jun. 1995 to Apr. 2000, 49 patients with HCC were undergone radical tumor resection. They were divided into 4 group: the treatment combined with HAI and PVI was given in group I(12 cases), HAV alone in group II (8 cases); PVI alone in group III (15 cases); nothing in group IV (14 cases). All patients were followed up for 6 months to 5 years. Results　Recurrence rate at one year didn't show significantly lower in group I, II and III than that in grou IV. Survival rate in one year showed no significant difference in 4 groups. Recurrence rate in three years was significantly lower in group I and II than that in group IV. Survival rate in three years was significantly higher in group I and II than that in group IV. Recurrence rate and survival rate in five years showed no significante difference in four groups. Conclusions　The treatment of radical resection of HCC combined with HAI and/or PVI is simple and safe. It is an useful method for preventing tumor recurrence.

Objective To study the effect of HAI and/or PVI after radical resection of hepatocellular carcinoma (HCC) for preventing recurrence.Methods From Jun. 1995 to Apr. 2000, 49 patients with HCC were undergone radical tumor resection. They were divided into 4 group: the treatment combined with HAI and PVI was given in group I(12 cases), HAV alone in group II (8 cases); PVI alone in group III (15 cases); nothing in group IV (14 cases). All patients were followed up for 6 months to 5 years. Results Recurrence rate at one year didn't show significantly lower in group I, II and III than that in grou IV. Survival rate in one year showed no significant difference in 4 groups. Recurrence rate in three years was significantly lower in group I and II than that in group IV. Survival rate in three years was significantly higher in group I and II than that in group IV. Recurrence rate and survival rate in five years showed no significante difference in four groups. Conclusions The treatment of radical resection of HCC combined with HAI and/or PVI is simple and safe. It is an useful method for preventing tumor recurrence.

The preparation of electrophoretic microcolumn and its application to the electrophoretic separation of amino acids with a 2-mm I.D. fused-silica microcolumn packed with uniform quartz microncrystal prepared by hydrothermal synthesis are reported. With 1.5 mmol/L disodium phosphate buffer solution (pH 11.5) containing 30% (V/V) methanol, the tryptophan, phenylalanine and tyrosine were separated by the microcolumn electrophoresis and detected by an UV spectrophotometer without derivatization. The limits of detection were 0.038, 0.21 and 0.20 mol/L, respectively. The separation efficiency of tryptophan was 4.4×104 plates/m. The sample capacity of the electrophoretic microcolumn achieved 35 μL. The precisions of the microcolumn electrophoresis were satisfactory. The thermal effects of the electrophoretic microcolumn that without packing, packed with 360 μm quartz sand and with 9 μm length quartz microncrystal were discussed, respectively. It was found that the electrophoretic microcolumn packed with quartz microncrystal was able to inhibit Joule heat, increase sample capacity and enhance detection sensitivity. The microcolumn electrophoresis is one of the high-performance separation techniques for an in-situ, real-time and portable electrokinetic flow analysis system.

The improvement of the electroosmotic pump properties and the effects of the bi-directional electrostacking system on the pre-concentration factor were investigated.A portable electrokinetic flow analysis system with electroosmotic driving and bi-directional electrostacking unit was introduced.The improved system with graphite furnace atomic absorption spectrometry was employed to separate,pre-concentrate and determine Cr(Ⅵ) and Cd(Ⅱ)in mineral water.The detection limit of Cr(Ⅵ)and Cd(Ⅱ)was 9 ng/L and 10 ng/L(3б of blank,n=11),respectively.Their recoveries of 200ng/L Cr(Ⅵ) and 100ng/L Cd(Ⅱ) added to samples were(105～107)±2% and (104～106)±2%(n=3).

Objective To evaluate the treatment effect of negative pressure wound therapy ( NPWT) followed by ultrasonic debridement surgical system ( UDSS) in patients with diabetic foot. Methods Forty-seven diabetic foot ulcer patients( WagnerⅡ~Ⅲ) were divided into two groups,of which 26 patients treated with UDSS combined NPWT,and 21 patients treated with traditional surgical debridement combined NPWT were control group. Cure rate, markedly effective rate and adverse reaction and complication were compared between two groups. Results Compared with control group,the experiment group had higher markedly effective rate in the first week(P<0. 05),and healing rates were higher in the 2th,4th,6th,12th weeks (P<0. 05). There were no differences in the adverse reactions and complications between the two groups. Conclusion UDSS combined with NPWT can accelerate wound cure,may play an important role for the treatment of diabetic foot ulcer.

Objective:To study the feasibility of laparoscopic anatomical hepatectomy of segment VII for hepatocellular carcinoma (HCC) with dorsal-ventral combined approach.Methods:Clinical data of 23 patients with HCC undergoing laparoscopic anatomical hepatectomy of segment VII with dorsal-ventral combined approach in Jiangnan University Affiliated Hospital from December 2020 to April 2023 were retrospectively collected, including 11 males and 12 females, aged (58.5±7.9) years old. The patient's perio-perative data, postoperative complications, and follow-ups were analyzed.Results:All patients underwent the surgery successfully without conversion to laparotomy. The operation time was (286.7±63.4) min, the amount of intraoperative blood loss [ M( Q1, Q3)] was 200(150, 400) ml without blood transfusion. There were no major complications such as abdominal bleeding, bile leakage, liver failure or abdominal infection. Two cases had right pleural effusion and were managed with thoracic drainage. The patients could ambulate on postoperative day 2, and the postoperative hospital stay was (9.36±1.72) d. The diameter of the lesion was (4.38±1.42) cm. The serum level of alpha fetoprotein (AFP) three months after surgery was (3.26±0.93) ng/ml, lower than the preoperative baseline (46.75±9.43) ng/ml ( t=38.24, P=0.008). All patients showed normal serum levels of AFP and there were no tumor recurrence or metastasis during postoperative follow-ups. Conclusion:Laparoscopic anatomical hepatectomy of segment VII for HCC with dorsal-ventral combined approach is feasible and worth spreading.

Objective To investigate the expressions of acid sensing ion channel 1a(ASIC1a) and activator protein-1 (AP-1) in hepatocellular carcinoma,and to analyze the relationship between expressions and clinical features.Methods At the transcription level,expression spectroscopy chips and bioinformatics were used to analyze the changes of signal pathways before and after ASICla interference in hepatocellular carcinoma cells.63 cases of HCC and 42 cases of tumor adjacent tissue samples were chosen from the Third People's Hospital of Wuxi,between January 2010 and December 2014.Immunohistochemical staining was utilized to detect the expressions of ASIC1 a,c-Jun,c-Fos.The relationships among the three were analyzed by the nonparametric Spearman rank correlation coefficient.Results After ASIC1a inhibition,the expression of AP-1 (c-Jun and c-Fox) decreased significantly.The expressions of c-Jun and c-Fox were greatly decreased in interference group compared with control group.The positive rates of ASIC1a,c-Jun and c-Fos in HCC tissues were significantly higher than that of the adjacent tissues,68.3％ vs.19.0％,55.6％ vs.11.9％,47.6％ vs.11.7％ (P ＜ 0.05).Correlation analysis showed that the expression of ASIC1a was positively correlated with c-Jun and c-Fos expression (r =0.404,0.309,P ＜ 0.05).The expressions of ASIC 1 a,c-Jun and c-Fos were not related to age,tumor diameter and gender (P ＞ 0.05),which were related to the clinical stage,AFP and lymph node metastasis of the tumor (P ＜ 0.05).Conclusion ASIC1a may.affect the development of hepatocellular carcinoma through the downstream gene AP-1.