1.Development and evaluation for volume overload-induced and pressure overload-induced cardiac hypertrophy in rats
Minli ZHANG ; Han XIAO ; Yan BAI ; Youyi ZHANG ; Zhaoping LI
Chinese Journal of Pathophysiology 1986;0(04):-
AIM: To compare the evaluations for the structure and function of the hypertrophic hearts induced by volume overload or pressure overload in rats. METHODS: Volume overload-induced cardiac hypertrophy was established by abdominal aortacaval fistula (ACF) and pressure overload-induced cardiac hypertrophy was developed by constriction of aorta (CA). The cardiac structure and function were analyzed by echocardiography, hemodynamic determination, heart weight measurement and histological examination. RESULTS: Heart weight of rats in all the operated groups was increased compared to the sham-operated groups. In 1-week ACF group, the internal diameter (0.67?0.03)cm vs (0.60?0.02)cm, P
2.Traditional Chinese medicine syndrome types and syndrome elements of nonalcoholic fatty liver disease
Gaiya GAO ; Sha LI ; Jingdong XUE ; Yanyan BAI ; Youyi HUI
Journal of Clinical Hepatology 2021;37(1):89-93
ObjectiveTo investigate the distribution of traditional Chinese medicine (TCM) syndrome types and syndrome elements of nonalcoholic fatty liver disease (NAFLD). MethodsRelated databases (CNKI, Wanfang Dota, and VIP)were searched for articles on the syndrome differentiation of NAFLD published up to July 2020. Two investigators independently performed literature screening and collection and summarization of syndrome types based on the inclusion and exclusion criteria, and an Excel 2010 database was established after the standardization of syndrome names, re-decomposition of syndrome types, and extraction of syndrome elements. The data were imported into SPSS 25.0 statistical software for the analysis of frequency distribution. ResultsA total of 45 qualified articles were collected, with a total of 8703 cases reported. A total of 14 syndrome types were obtained after standardization, and 10 syndrome elements reflecting the nature of disease and 4 syndrome elements of disease location were obtained after the syndrome types were disassembled. Stagnation of liver Qi and spleen deficiency syndrome (26.47%) and damp-heat accumulation syndrome (22.16%) were the most common syndrome types, followed by stagnation of phlegm dampness, intermingled phlegm and blood stasis, and stagnation of liver Qi and Qi stagnation. Dampness (23.75%), Qi stagnation (19.82%), Qi deficiency (17.12%), phlegm (15.43%), and heat (12.13%) were the most common syndrome elements reflecting the nature of disease, followed by stasis, Yin deficiency, and Yang deficiency, while fire and cold were relatively uncommon. Qi stagnation and Qi deficiency (26.63%), dampness and heat (22.30%), phlegm and dampness (16.17%), and phlegm and stasis (12.19%) were the most common combinations of syndrome elements. The liver and the spleen were the most common syndrome elements of disease location, accounting for 90.95% of the constituent ratio, and the combination of the liver and the spleen with the same disease accounted for 54.01%. The combination of one, two, three, or four syndrome elements was observed, and the combination of two syndrome elements accounted for 76.03%. ConclusionStagnation of liver Qi and spleen deficiency are the basic pathogeneses of NAFLD, and liver, spleen, dampness, Qi stagnation, Qi deficiency, phlegm, and heat are common syndrome elements. Dampness, phlegm, and heat are important factors for the development and progression of this disease.
3.Comparison of arterialized venous flap with different perfusion strategies for repairing hand soft tissue defects
Ju GUO ; Da LI ; Xiaofeng CHEN ; Youyi BAI
Chinese Journal of Postgraduates of Medicine 2019;42(8):731-734
Objective To compare the clinical effects of of arterialized venous flap (AVF) with different perfusion strategies for repairing hand soft tissue defects. Methods Ninety-six patients with hand soft tissue defects from January 2015 to June 2018 in Gaozhou Traditional Chinese Medical Hospital were collected and randomly divided into two groups: the inverted group (48 patients) treated with anti-valve arterialized venous flap inverted anastomosis and the non-inverted group (48 patients) receiving pro-valve arterialized venous flap non-inverted anastomosis. The excellent and good rate of hand function recovery, flap survival rate,postoperative skin flap response degree and treatment satisfaction between two groups were compared. Results Compared with the inverted group, the flap survival rate in the non-inverted group increased significantly [97.9% (47/48) vs. 83.3% (40/48)], the proportion of mild flap response degree increased [45.8% (22/48) vs. 18.8% (9/48)], the proportion of severe flap response degree decreased significantly [12.5%(6/48) vs. 41.7%(20/48)], and the differences were statistically significant (χ2=4.391, 7.032, 8.205, P < 0.05). The excellent and good rate of hand function recovery in inverted group and non-inverted group had no significant differences (χ2=1.233, P>0.05). There was no significant difference in satisfaction with the shape of skin flaps, finger function and donor site recovery between two groups(P>0.05). Conclusions Compared with anti-valve arterialized venous flap inverted anastomosis, pro-valve arterialized venous flap non-inverted anastomosis on soft tissue defect repairment of hand can efficiently increase the survival rate of flap and improve the flap response degree after operation.
4. Mechanism of angiotensin II (Ang II) on the proliferation of human hepatoma cell line HepG2 cells
Ran QI ; Chenggang LEI ; Yixuan BAI ; Xue XING
Chinese Journal of Hepatology 2018;26(8):601-606
Objective:
To study the effect and mechanism of angiotensin (Ang II) on the proliferation of human hepatocellular carcinoma HepG2 cells.
Methods:
The effects of different concentrations of Ang II's (10-8-10-4 mol/L) on proliferated hepatocellular carcinoma HepG2 cells were detected by CCK-8 assay. The expression of angiotensin II type 1 receptor (AT1) protein and activation of ERK1/2 protein in hepatocellular carcinoma HepG2 cells after processing with Ang II were assayed by Western blot. The cells were pretreated with candesartan (AT1 receptor antagonist), sorafenib (Raf kinase inhibitor) and PD98059 (ERK1/2 inhibitor) for 1.5 h and then Ang II (10-6 mol/L) was added. CCK-8 assay was used to determine whether it could reverse the proliferation of Ang II, and ERK phosphorylation levels were detected by Western blot. The changes in Bcl-2 and c-myc gene expression before and after Ang II processing were detected by Rt-PCR. According to different data,