1.The effect of intracerebroventricular microinjection of pentagastrin on rat's gastric electricity and gastric movement
Chinese Journal of Pathophysiology 1989;0(05):-
The effect of intracerebroventricular microinjection of 0.1?g pentagastrin(G-5) on rat's gastris electricity and gastric movement were observed. The results showed that after intracerebroventricular microinjection of G-5 (0.1?g/10?l), the amplitude and frequency of rat's gastric electricity slow wave were increased from the control value of 0.56mV to 0.847mV (P
2.Certification of linear range of WBC measured by CD3700 and application in ISO15189
Youye YANG ; Zhizhao YANG ; Lishao MIAO ; Fuda HUANG ; Jinsheng LIANG
Basic & Clinical Medicine 2006;0(10):-
Objective To certify the linear range of WBC measured by CD3700.Methods Use the white stratum of blood after centrifugation.Then,the high value specimens were diluted with saline to get a series of samples with different concentrations.Every specimen was measured twice in order to keep the sequence of specimens randomly.The perform outlier test,polynomial regression analysis,and the random error estimation were carried out.Results There was no outlier in the results.It was demonstrated by polynomial regression to be linear from(0~219)?109 L-1.The random error was within the allowable limit of 1/4CLIA'88(3.75%).Conclusion The linear range of WBC measured by CD3700 is just like that of the manufacturer claims.
3.Investigation of validation for biology reference interval of clinical biochemical testing
Guishan CHEN ; Youye YANG ; Dongmei WEN ; Lishao MIAO ; Jinsheng HANG
Chinese Journal of Laboratory Medicine 2008;31(2):170-174
Objective To validate the biology reference interval of the Dartial biochemistry test items and provide accurate diagnosis basis for the clinic.Methods According to NCCLS C28-A2 recommendation method,two biochemistry analyzers'performance was validated.20 healthy persons for the asexual biological difference project and female group as well as male group (each group consist of 20 persons)for the sex biology difference project were recruited according to the laboratory SOP.If the validation had doubt,other two groups were need.The sera from these individuals were examined by the full-automatic ADVIA 1650 biochemistry analysis system.Results Two full-automatic ADVIA 1650 biochemistry analyzes conformed to the requirements.In 16 test items participating in this investigation.the analysis showed more than 5%results fell outside the biology reference interval for GGT and HDL-C,whereas 95% results fell in the biology reference interval for TP.All other results fell in the biology reference interval.Conclusions This validation indicats that except GGT and HDL-C,the biology reference intervals which are currently being used are suitable for our laboratory.This verification is persuasive and calpable of finding the deviation of biology reference interval.Setting up verification system warrants further generalization.
4.Clinical laboratory management using ISO15189
Youye YANG ; Jinsheng LIANG ; Zhizhao YANG ; Guishan CHEN ; Manzhang CHEN ; Dongmei WEN ; Changhui CAI
Basic & Clinical Medicine 2006;0(01):-
Patient,competition and change are characteristics of the modern hospital manage environment,clinical laboratory management must be more scientific and more religious.This article lists the main items of clinical laboratory management using ISO15189,and raised some idea on clinical laboratory management using ISO15189 and points out the difficulties of popularization using ISO15189 in clinical laboratory management.