Objective To explore the diagnostic value of the ECG treadmill exercise test in women with coronary heart disease.Methods The clinical data of 108 female patients who received the electrocardiogram treadmill exercise test and coronary angiography and suspected coronary heart disease were analyzed.The results of treadmill exercise test and coronary angiography were comparatively analyzed.Results Treadmill exercise test sensitivity was 57.64％ (49/85),specificity was 73.46％ (36/49),positive predictive value was 79.03％ (49/62),negative predictive value was 78.26％ (36/46),accuracy was 78.70％ (85/108).False-positive group exercise time,ST-segment depression amplitude,duration,number of leads were lower than the true-positive group (t =2.49,6.08,4.68,8.00,all P ＜0.05).Coronary artery stenosis in patients with ECG treadmill exercise test ECG display the difference was statistically significant.The more serious coronary artery stenosis lesions electrocardiogram showed lack of blood type ST-segment depression appear sooner,the longer the duration,the greater the magnitude of depression.Conclusion Treadmill exercise is an ideal noninvasive method in the diagnosis of women with coronary heart disease.

Immunotherapy is becoming an effective and less invasive strategy that can be applied to the treatment of various malignancies. Lentiviral vectors (LVs) have shown great potential in immunotherapy as they can stably integrate relatively large foreign DNA, and effectively transduce dividing and non-dividing cells. Clinical application needs high quality LVs, and therefore strict quality control of the final products is necessary to ensure their purity, efficacy and safety. The quantitative detection of LVs is among the key parts of product development and quality control. In this paper, the existing methods for quantitative detection of LVs are summarized, including fluorescence activated cell sorter (FACS), P24 enzyme-linked immuno sorbent assay (P24 ELISA), real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), nanoparticle tracking analysis (NTA), tunable resistive pulse sensing(TRPS) and virus counter(VC).Their advantages and disadvantages are listed, and future development and challenges are discussed.
Genetic Vectors/genetics* ; Humans ; Immunotherapy ; Lentivirus/genetics* ; Neoplasms ; Transduction, Genetic