A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria; (20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs (4.4x105 and 2.6x104 colonies/1 g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group (20.0%) and Trichoderma (14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus (telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique.
Aspergillus ; Austria ; Chickens ; Gizzard ; Heart ; Intestines ; Liver ; Random Amplified Polymorphic DNA Technique* ; Spleen ; Trichoderma ; Viscera

Gibberella fujikuroi is species complex. This species complex includes Fusarium tabacinum, F. moniliforme (= F. verticillioides), F. nygamai, F. proliferatum as well as F. subglutinans. Our objective was to develop a technique to differentiate between isolates of F. subglutinans, F. proliferatum and F. verticillioides. Thirty-two strains of F. subglutinans, six strains from F. verticillioides and five strains of F. Proliferatum isolated from maize in Austria were studied using random amplified polymorphic DNA (RAPD). F. subglutinans strains clustered very closely, with similarity ranging from 87~100%. On the other hand, all the amplification patterns of F. verticillioides were identical, as well as in the case of F. proliferatum. Our results indicated that these Fusaria species are distinct species and hence RAPD markers can be quick and reliable for differentiating them.
Austria* ; DNA ; Fusarium* ; Gibberella ; Hand ; Random Amplified Polymorphic DNA Technique ; Zea mays*

The mycobiota of 160 hair and nail samples collected from 4 different governorates in upper Egypt were estimated using soil plate method for isolating keratinophilic and dermatophytic fungi. Twenty- three fungi were recorded on both hair and nail samples collected from the four governorates. Highest fungal diversity (20) was collected from Red Sea samples followed by Qena (18) and Aswan (17) while lowest fungal diversity was recorded from Sohage samples. The common genera were Aphanoascus, Aspergillus, Penicillium, Paecilomyces and Chrysosporium. The most prevalent species belonging to these genera were: A. fulvescens, Aphanoascus sp. A. flavus link, A. flavus var. columnaris, P. chrysogenium. P. lilacinus and C. sulfureum. True dermatophytes such as Nannizzia fulva appeared in 20~30% of the male samples.
Arthrodermataceae ; Aspergillus ; Chrysosporium ; Egypt* ; Fungi* ; Hair* ; Humans* ; Indian Ocean ; Male ; Paecilomyces ; Penicillium ; Soil