BACKGROUND:Surgical treatment is mostly adopted in the treatment of III-IV pelvic organ prolapse, in which, the appropriate patch can be used to implement the pelvic floor reconstruction so as to reconstruct the pelvic anatomic relationship and restore the normal pelvic organ function. OBJECTIVE: To verify the efficacy and biocompatibility of the non-absorbable mesh reconstruction of the pelvic floor in III-IV pelvic organ prolapse. METHODS: Totaly 127 female cases of III-IV pelvic organ prolapse were enroled, aged 31-81 years. Of the 127 cases, 65 cases undergoing vaginal hysterectomy and anterior and posterior vaginal wal repair served as control group, another 62 cases undergoing pelvic floor reconstruction using non-absorbable mesh acted as observation group. The two groups were observed in the perioperative period and then folowed up for 12 months. POP-Q measurement, pelvic floor function and adverse reactions were observed in the two groups. RESULTS AND CONCLUSION:The operation time and blood loss in the observation group were less than those in the control group (P < 0.05). After 12 months of folow-up, the results of POP-Q measurement were significantly improved in the both groups compared with the previous (P< 0.05), but there was no difference between the two groups. In addition, pelvic floor static tension, dynamic tension and contraction force were stronger in the observation group than the control group (P < 0.05). In the observation group, there were six cases of mesh corrosion, one case of infection and one case of dysuria. It is indicated that the non-absorbable mesh reconstruction of the pelvic floor can effectively restore the pelvic energy in patients with III-IV pelvic organ prolapse, but there are some adverse reactions such as mesh corrosion.

Object To establish an HPLC method for the determination of dehydroxypresenegenin for the quality control of Polygalae tenuifolia Willd. Methods Dehydroxypresenegenin was separated by ODS column (125 mm ? 4 mm, 5 ?m) with a mobile phase of methanol 0 05% phosphoric acid (74∶26) and detected at a wavelength of 210 nm. Results The calibration curve was linear in the range of 0 585～11 7 ?g,with a correlation coefficient of 0 999 99 . The average recovery was 99 21% (n=5), and RSD=0 25% (n=5). Conclusion The method is simple and accurate, with good repeatability and can be used for the quality control of Polygalae tenuifolia Willd and compound preparations of Radix Polygalae.