Objective To investigate the effect of combined traditional chinese medicine and western medicine in treatment old men with femoral trochanteric fracture. Methods120 patients with femoral trochanteric fracture were separated into two groups.Patients in the experiment group were managed by traditional Chinese treatment besides the operations and patients in the control group received only operation treatment.We observed the patients' time of fracture healing and the life quality of them. ResultsThe cure effect of observe group was higher than that of control group(P＜0.05)and the fracture healing time in the observe group was shorter than that of control group(P＜0.01). ConclusionCombined treatment of traditional Chinese medicine and western medicine was more effective in promoting the physical reconstruction of patients with femoral trochanteric fracture.

Fungal infection of lung has been a common disease.The complicated clinical manifestations make its diagnosis and treatment difficult.The following should be paid attention to.First,different pulmonary mycosis may have very different clinical characteristics,so the diagnostic standard and therapy should be established accordingly.Second,the proven cases of candida pneumonia are decreasing,probably because of the decreasing needs for biopsies.So candida pneumonia seems still common.Third,different types of endemic pulmonary mycosis have distinguishing characteristics.Some cases could be misdiagnosed as cancers or other diseases,which we should be aware of.

To investigate the possibility to enhance resistance of human bone marrow cells to anticancer agents by transfection of mdrl gene. We transferred mdrl gene into human bone marrow cells with plasmid pHaMDR1/A containing human mdrl cDNA by Lipofectin.Pgp,mdrl gene expression product was detected by both flow cytometry and immunohistochemistry.Also,Pgp function was tested by efflux study using rhodamine.The resistance of human bone marrow cells to anticancer agents was assayed by color forming unit culture after exposure to Vincristine,Daunomycin, and Vp16.The results showed that mdrl gene was successfully transferred into human bone marrow cells, and it expressed.Biological activity of Pgp was confirmed by efflux study using rhodamine.Transferred human bone marrow cells possessed resistance to anticancer agents.It suggested that transfection of mdrl gene can increase resistance of human bone marrow cells to anticancer agents.

OBJECTIVE To study the synergism of imipenem(IMP) combined with azithromyicn(AZM) in the treatment of infection associated with Pseudomonas aeruginosa biofilm.METHODS The inhibitory activity of AZM to glycocalyx(GLX) production was detected with the L-tryptophan method.Viable counts in biofilms treated with AZM combined with IMP were detected with the methyl thiazolyl diphenyltetrazolium(MTT) method in vitro.The tissue cage method was used to establish the animal model of local P.aeruginosa biofilm infection and the synergism of IMP combined with AZM was also studied in vivo.RESULTS AZM could inhibit the production of GLX.When IMP was combined with 1/4MIC or 1/16MIC of AZM viable counts in biofilms were less than they were when IMP was given alone in vitro.When AZM and IMP were administered at the same time viable counts in tissue cage fluids were also less than they were when IMP was administered alone in vivo.CONCLUSIONS AZM could enhance the antibacterial activity of IMP in the treatment of infection associated with biofilm.

OBJECTIVE To study the prevalence of atypical pathogens in patients with community acquired pneumonia(CAP) and the distribution of serotypes of Legionella pneumophilia.METHODS A prospective study was performed on 257 consecutive adult patients with CAP between Dec 2003 and Nov 2004.Antibodies of the paired serum samples to Mycoplasma pneumoniae,Chlamydia pneumoniae and Legionella pneumophilia serotypes 1 to 14 were detected.RESULTS The etiology of CAP was identified in 82(31.9%) patients.The distribution of causal agents was as follows:M.pneumoniae 63(24.5%),C.pneumoniae 17(6.6%),and L.pneumophilia 11(4.28%).Serotype 12 of L.pneumophilia was the predominant one,responsible for 21.8%(56/257).CONCLUSIONS Atypical pathogens especially M.pneumoniae have an important role in CAP;serotype 12 of L.pneumophilia is the predominant one in northern area of China.

Objective To discuss the feasibility and clinical significance of detecting circulating tumor cells(CTCs) in peripheral blood of patients with lung cancer in early stage. Methods Three groups of patients (Group A: 15 cases with early stage lung cancer; Group B: 20 cases with benign pulmonary disease; Group C: 20 healthy volunteers) were enrolled for detection of CTCs using immunomagnetic separation(IMS)+ immunocytochemistry(ICC) method and RT-PCR method. The patients in Group A were followed up for 6-9 months. Results By using IMS+ICC it was revealed that in 5 cases of Group A the result was positive, while it was negative in all cases of Group B and Group C. By using RT-PCR method, the result was positive in 7 cases of Group A, 8 cases of Group B and 6 cases of Group C. In the follow-up period, 2 patients of Group A, who were found positive for CTC before and after operation, showed recurrence. Conclusion IMS + ICC method was a sensitive method to detect CTCs in patients with early stage NSCLC. The detection of CTCs in patients with early stage lung cancer might be a relationship with the clinical prognosis of the patient.

Objective To study the changes in serum SARS coronavirus (SARS CoV) IgG antibody, and lung function and radiographic image of the lung six months after being discharged from the hospital. The incidence of femoral head necrosis in some of them was also evaluated. Methods 293 patients who were convalescent from SARS regularly received SARS CoV IgG antibody assay, lung function test and chest X ray and/or high resolution computerized tomography (HRCT) examination in the General Hospital of PLA 6 months after being discharge. In the patients who were found to have lung diffusion abnormity (D L CO

0.01), no significant differences were observed. The peak SBA of CPZ/SBT and CPZ/TZBT against ESBLs positive K. pneumoniae and E. coli were shown to be slightly stronger than that of CPZ by 2-4 times (P

OBJECTIVE To determine the activity of moxifloxacin on bacterial biofilms formed by Haemophilus influenzae in vitro.METHODS Formation of bacterial biofilm was examined by crystal violet assay,viable cells counting in biofilms was also carried out.Alterations of biofilms were measured under varying concentrations of moxifloxacin.RESULTS Optical density values of biofilms were significantly decreased at the concentrations higher than 4 mg/L of moxifloxacin.The similar results were obtained for viable cell counting.Bacteria in biofilms were eliminated partly or completely at concentrations higher than 0.25 mg/L.CONCLUSIONS Moxifloxacin is able to destroy the biofilms and reduce viable cells counting at high concentrations.

Objective To study the correlation between mutant selection window (MSW) and selective enrichment of Staphylococcus aureus resistant mutants in vivo. Methods Tuberculosis patients colonized with S. aureus in anterior nares were selected as experimental group. The susceptibility of S. aureus to rifampicin was determined at the beginning of and 2,4 and 5 weeks after the anti-tuberculosis therapy with rifampicin-containing regimens. S. aureus isolates developing acquired resistance were examined by molecular strain typing. Diabetes patients colonized with S. aureus served as an untreated control. Results The S. aureus isolated from 5 patients acquired rifampicin resistance in 58 tuberculosis patients. It was determined by pulsed-field gel electrophoresis and protein A repeat sequence the strains of S. aureus were different, but the isolates obtained from the same patient before and after acquisition of resistance were the same strains. No resistance was acquired in 39 untreated control patients, which differed statistically from treated patients. Conclusion The selective enrichment of rifampicin-resistant S. aureus mutants occurred when rifampicin concentration was in the mutant selection window.