No abstract available.
Licensure*

It is still not well documented what the basic roles for the regulation of renin secretion from the kidney take place. Since the early study on the renal ischemia for the production of hypertension was introduced, the renin-angiotensin system has been regarded as the possible pathogenetic mechanism for the renovascular hypertension. The renin-angiotensin system, however, could be activated by various stimuli, such as, the changes of intrarenal perfusion pressure, the load or concentriation of sodium at the sites of macular densa, the changes of the sympathetic nervous activity and the changes of potassium balance. To investigate the renin-angiotensin system and the influence of sympathetic nervous system on the regulation of renin secretion, the renovascular hypertension was induced in the dogs by constriction of unilateral renal artery, and the plasma renin activity was measured. The sodium load at the sites of macula densa was attained by furosemide, and then the activity of sympathetic nervous system was depressed by reserpine. The plasma renin activity was assayed by the method of Helmer and Cohn. By this bio-assay method, the plasma renin activity equivalent to 1 nanogram angiotensin-II can be measurable and the prepared plasma was found to have still vasopressor activity. The results observed in this experimental work are summarized as follows. 1. The blood pressure reached maximum on the 3rd postoperative day, and declined gradually to the level of preoperative day on the day of from the 6th to 12th day following constriction of renal artery. 3. The plasma renin activity was found to be well correlated with the increase of blood pressure, and then declined to its preoperative level with the reduction of blood pressure. It appears, therefore, that the renin-angiotensin system plays an important role in the pathogenesis of renovascular hypertension. 4. The urine flow rate in normal and reserpinized dogs was the same before administration of furosemide, but its rate was significantly increased in both groups after furosemide. The urine flow rate of reserpinized dogs, however, was significantly lower than that of the normal dogs during the first 5 minutes. 5. The urinary sodium excretion in normal and reserpinized dogs was significantly increased in both groups after administration of furosemide and there were no difference between the two groups. 6. The plasma renin activity of renal venous blood was significantly higher than that of femoral arterial blood in both groups before and after administration of furosemide. 7. The plasma renin activity of reserpinized dogs was significantly lower than that of normal dogs before administration of furosemide. After furosemide, however, the plasma renin activity was significantly increased in 30 minutes in both groups. This increase of plasma renin activity was less prominent in resepinized dogs than in normal. This results would suggest that the intrarenal mechanism regulating renin secretion also requires an intact sympathetic nervous system.
Animals ; Blood Pressure ; Constriction ; Dogs ; Furosemide ; Hypertension ; Hypertension, Renovascular ; Ischemia ; Kidney ; Perfusion ; Plasma ; Potassium ; Renal Artery ; Renin* ; Renin-Angiotensin System ; Reserpine ; Sodium ; Sympathetic Nervous System

No abstract available.
Breast Neoplasms* ; Breast*

No abstract available.
Diagnosis* ; Gout*

During the period of 1964 to 1972, epididymovasoetomies were performed on 53 cases of azoospermias due to epididymal obstruction. They were operated by the technics of the ordinary side-to-side epididymovasostomy with splint, in such a manner that a minute longitudinal opening in the vas was anastomosed to a similar opening in the epididymis at a site where live sperm had been recovered, which was applied on 58 per cent of the subjects, and of the buried side-to-side anastomosis with a splint, wherein longitudinally opened vas ..was actually buried in longitudinally opened head of epididymis, which was attempted on 26 per cent of them. The following data are presented as abstraction of clinical findings and the operation outcomes: l. An average age of the subjects was 35, and that of their wives, 30. 2. An average duration intervening between the beginning of the obstruction and the performance of the operation was 9 years. 3. An average frequency of sexual intercourse per week indicated 2.5 times before the obstruction 2. 2 times after the obstruction, and 2. 1 times after the operation. 4. In the majority of the cases, the post-inflammatory obstructions were found to be the most responsible causes for azoospermia in my present study. That is, non-tuberculous epididymitis (gonorrheal and non-gonorrheal origin) was found in 70 per cent of the subjects and tuberculous origin, 25 per cent of them respectively. 5. Success rate of non-tuberculous epididymitis group is higher than that of tuberculous epididymitis counter part 6. Success rate of the buried technic group is higher than that of the ordinary technic group. 7. Success rate of bilateral oozes with sperm group is higher than that of unilateral oozes with sperm or no oozes group. 8. A total of 53 cases was operated upon for curing the obstructive azooepermias. Poet-operative semen analyses were carried out on 43 cases out of the 53. Sperm appeared in the ejaculates of 17 cases out of the 43 in repeated sperm tests 3 weeks following the operation. Accordingly, successful operations were showed in 40 per cent of the 43 cases. Seven pregnancies(16%) followed the operation. 9. The writer believes that epididymovasostomy could be said to be a worthwhile procedure for the cases of asooapermia due to the obstruction of globus minor with normal spermatogenesis. The prognosis is always good whenever the indications are strictly followed.
Azoospermia ; Coitus ; Epididymis ; Epididymitis ; Head ; Humans ; Male ; Prognosis ; Semen Analysis ; Spermatogenesis ; Spermatozoa ; Splints ; Spouses

Three point scoring systems for the diagnosis of left ventricular hypertrophy were studied referring to positivity as well as false positivity in 100 cases of clinically proven LVH and 100 cases of healthy persons. 1) By Estes' scoring system 98% of LVH cases were diagnosed as LVH. There was 14% of false positive rate in healthy person. 2) By Romhilt and Estes' scoring system, 58% of LVH cases were diagnosed as LVH. There was 1% of false positive rate in healthy person. 3) By Skjaggested and Kierulf scoring system, 81% of LVH were diagnosed as LVH. There was 17% of false positive rate in healthy person. 4) Point scoring systems were more accurate in electrocardiographic diagnosis for LVH than any other conventional criteria. Among the reported scoring systems, Estes scoring system was considered the most useful as well as accurate one.
Diagnosis* ; Electrocardiography* ; Humans ; Hypertrophy, Left Ventricular*

To clarify the physiological thermoregulatory response of the human body, the local heating and hunting reaction experiments were performaed. The subjects were 10 healthy women from 19 to 22 years of age. For the local heating experiment, oral temperature, skin temperature, deep skin temperature and thermal sensations were measured every 5 min during the 40 min experiment. Heated areas were neck, shoulder, abdomen, hands and feet. These five areas were heated with the hot pack of 50℃. For the hunting reaction experiment, oral temperature, thermal sensation of the whole body and cold pain sensation of the finger were measured every 5 min during and after immersion of the left middle finger in 5℃ ice water for 40 min. The skin temperature of the finger tip was measured with a copperconstantan thermocouple every each min during and after immersion. Cold tolerance index was made on the basis of following items ; the skin temperature at the first rise(T.F.R), time for the first skin temperature rise (T.T.R), mean skin temperature during immersion(M.S.t). Conclusions : 1. Skin temperature and deep skin temperature have been much influenced by the abdomen heating. 2. The correlation between the whole body thermal sensation and the upper trunk thermal sensation was singnificantly recognized. 3. T.F.R. and M.S.T. of the hunting reaction with abdomen heating is significantly higher at the 0.01 level by T-test than that of the hunting reaction without abdomen heating. T.T.R is also significantly shorter for the hunting reaction with abdomen heating than that for the hunting reaction without abdomen heating.
Abdomen ; Female ; Fingers ; Foot ; Hand ; Heating* ; Hot Temperature* ; Human Body ; Humans ; Ice ; Immersion ; Neck ; Sensation ; Shoulder ; Skin Temperature ; Water

Cyclosporine A (CSA), a lipophilic cyclic undecapeptide, is not accumulated evently in all tissues and has a high affinity to several tissues such as lymphoid organs, liver, and kidneys. From this point of view, it is reasonable to assume that the amount of CSA uptake would be correlated with the extent of cell injury. On the other hand, verapamil, a Ca2+ channel blocker, bas been shown to ameliorate CSA nephrotoxicity. Since proximal tubule is the major site of drug transport and CSA uptake and its interaction with verapamil in isolated human renal proximal tubular cells. The CSA uptake rapidly increased over the first 5 min and then achieved almost steady-state after 10 min at all concentrations (0.5-10 uM). Kinetic analysis yielded that the Km and Vmax values of CSA were 5.6 uM and 86.2 p mol/mg cell protein/min, respectively. And Ca2+ depletion in media enhanced CSA uptake significantly but verapamil reduced it. These results suggest that the Ca2+ channels and CSA transporting sites on cell membrane are closely associated and that Ca2+ and CSA might be taken up competitively by proximal tubular cells.
Humans

Nephrotoxicity is the most common dose-limiting factor of cyclosporine A (CSA) in clinical usage. But the mechanism of CSA-induced nephrotoxicity still remains unresolved. Many authors insisted that CSA induced renal proximal tubular cell injury is due to the secondary effects following hemodynamic changes or endothelial cell damage, instead of direct toxicity by CSA. To find out that CSA has a direct toxicity to the proximal tubular cells, the author used primary cultures of human proximal tubular cells to eliminate the hemodynamic or endothelial influences that could be produced in in vivo model. In the present study, the viability against CSA was tested by the neutral red assay method with modulation of Ca2+ amount in incubating media and observed electron microscopically. The viability test showed direct toxic effect of CSA on human proximal tubular cells and this was enhanced by Ca2+ depletion in incubating media. Morphologically noted are accumulation of lipid droplets and polyribosomal dispersion, which may be association with inhibition of cellular synthetic activity. These results suggest the toxixity is a direct effect of cyclosporine and that toxic mechanism may be due to inhibition of cellular synthetic activity. And this experiment also showed that primary cultures of human renal proximal tubular cells can be a good in in vivo model for investigating CSA nephrotoxicity.
Humans

No abstract available.
Extracorporeal Membrane Oxygenation*