1.Laminin B1 mRNA expression in squamous cell carcinoma of the skin.
Kyu Suk LEE ; Youn Ji RHO ; Joo Young SONG
Korean Journal of Dermatology 1993;31(2):157-162
BACKGROUND: Laminin, which is present in all basement membrane, is a multifunctional glycoprotein with diverse biological activities. Like fibronectin, it can influence cell adhesion, growth, morphology, differentiation, and migration as well as the assembly of the extracellular matrix. Laminin primarily affects cells of epithelial origin, and response varies depending on the cells. OBJECTIVE: Studies about the relationship of laminin and the pathogenesis or metastasis of squamous cell carcinoma(SCC). MEHTODS: In this study, we have examined the laminin B1 mRNA expression and localizing pattern of laminin in SCC, using Northern and slot-blot hybridization, in situ hybridization and immunoperoxidase stain. RESULTS: In Northern blot hybridization, 5.6Kb mRNA transcript was detected in control and SCC specimens. Slot-blot hybridization indicated theat amounts of laminin B1 mRNA expression in SCC was 677.0+/-268.5 densitometer absorbance unit, in control was 228.0+/-16.37 unit respectively. There is a 3-fold increase in SCC compared to control. In in situ hybridization, 35S-labeled laminin B1 cDNA-mRNA hybrid granules were detected along the basement membrane and adjacent cells of SCC. In immunoperoxidase stain, laminin has stained strongly and continuously along the basement membrane of the tumor nest in well differentiated SCC, and weakly and interrupted in poorly differentiated SCC. CONCLUSION: These results suggested that laminin might be related to the pathogenesisand metastasis of squamous cell carcinomas.
Basement Membrane
;
Blotting, Northern
;
Carcinoma, Squamous Cell*
;
Cell Adhesion
;
Extracellular Matrix
;
Fibronectins
;
Glycoproteins
;
In Situ Hybridization
;
Laminin*
;
Neoplasm Metastasis
;
RNA, Messenger*
;
Skin*
2.Clinical Effects of Ursodeoxycholic Acid on Total Parenteral Nutrition Induced Cholestasis in Premature Infants.
Eun Song SONG ; Ji Youn KIM ; So Youn KIM ; Hyung Suk BYUN ; Young Youn CHOI
Journal of the Korean Society of Neonatology 2004;11(2):210-217
PURPOSE: Cholestatic liver disease is a frequent complication of prolonged parenteral nutrition, especially in premature infants. The purpose of present study is to evaluate the clinical and biochemical efficacy of ursodeoxycholic acid (UDCA) in premature infants with total parenteral nutrition (TPN) cholestasis. METHODS: Retrospective chart review of 31 prematures with TPN cholestasis in Neonatal Intensive Care Unit of Chonnam University Hospital from January 1995 and December 2002 was done. Prematures were divided into two groups based on UDCA treatment: the study group (n=22, with UDCA treatment) and the control group (n=9, without UDCA treatment). Treatment efficacy of UDCA was evaluated by monitoring the changes of serum direct bilirubin level for more than one to two months. RESULTS: In study group, cholestasis appeared at a mean age of 23+/-18.8 days after a mean of 21+/-19.7 days of TPN. UDCA was initiated (ranged 15-30 mg/kg/day) at a mean age of 54+/-22.3 days for a mean of 32+/-19.0 days. There was no significant difference in decrease of direct bilirubin level between the study and control group. However, the day of initial enteral feeding and full enteral feeding were earlier in control group compared with study group. CONCLUSIONS: This study shows that UDCA treatment is not effective in the treatment of TPN cholestasis among premature infants unlike that of results reported in children and adults. To reduce the incidence of TPN cholestasis in premature infants, early introduction of enteral feeding as soon as possible is most important.
Adult
;
Bilirubin
;
Child
;
Cholestasis*
;
Enteral Nutrition
;
Humans
;
Incidence
;
Infant, Newborn
;
Infant, Premature*
;
Intensive Care, Neonatal
;
Jeollanam-do
;
Liver Diseases
;
Parenteral Nutrition
;
Parenteral Nutrition, Total*
;
Retrospective Studies
;
Treatment Outcome
;
Ursodeoxycholic Acid*
3.Perinatal Prognosis of Single Umbilical Artery.
Yoon Ha KIM ; Tae Bok SONG ; Ji Soo BYUN ; Young Youn CHOI ; Ji Young LEE
Korean Journal of Perinatology 1999;10(2):155-160
OBJECTIVE: To evaluate the perinatal prognosis of fetuses with a single umbilical artery. METHODS: From 1992 to 1998, nineteen cases with single umbilical artery(SUA) was observed in 8,704 deliveries at Chonnam University Hospital. RESULTS: Out of nineteen fetuses, thirteen fetuses with single umbilical artery were detected by antenatal ultrasonographic examination and six fetuses were detected after birth. The male to female ratio was 0.9: 1. Congenital malformations were observed in 8 babies(42.1%) and included leg deformity, esophageal atresia, imperforated anus, ventriculomegaly, meningocele, hydronephrosis, ventricular septal defect, joint contracture, cleft lip and palate, toe anomaly, imperforated anus, kyphosis, no urethra and testis, clubfoot, patent ductus arteriosus and rnild mitral regurgitation. Among 10 cases of karyotyping analysis three cases were diagnosed as trisomy 18. Fourteen fetuses(77.8%) showed growth restriction at delivery. Antenatal obstetric complications were hydramnios(n = 3), oligohydramnios(n =2), and severe preeclampsia(n = 3). CONCLUSION: Careful ultrasonographic evaluation for the identification of a SUA is necessary because of its frequent association with congenital anomaly, growth restriction and cytogenetic abnormality.
Anal Canal
;
Chromosome Aberrations
;
Cleft Lip
;
Clubfoot
;
Congenital Abnormalities
;
Contracture
;
Ductus Arteriosus, Patent
;
Esophageal Atresia
;
Female
;
Fetus
;
Heart Septal Defects, Ventricular
;
Humans
;
Hydronephrosis
;
Jeollanam-do
;
Joints
;
Karyotyping
;
Kyphosis
;
Leg
;
Male
;
Meningocele
;
Mitral Valve Insufficiency
;
Palate
;
Parturition
;
Prognosis*
;
Single Umbilical Artery*
;
Testis
;
Toes
;
Trisomy
;
Urethra
4.A case of prenatal ultrasonographic diagnosis of Roberts syndrome.
Yong Hoon CHO ; Young Don YOON ; Tae Bok SONG ; Ji Soo BYUN ; Young Youn CHOI
Korean Journal of Perinatology 1992;3(1):88-94
No abstract available.
Diagnosis*
5.Usefulness of Routine Renal Sonography Screening for Healthy Infants with Isolated Single Umbilical Artery .
Ju Mi KANG ; Eun Song SONG ; Jin Hwa KUK ; Hyung Sin LEE ; So Young KIM ; Young Youn CHO
Journal of the Korean Pediatric Society 2003;46(9):854-857
PURPOSE: It is known that single umbilical artery is frequently associated with gastrointestinal or urogenital anomaly, however, routine renal sonography has been debated in healthy neonate with isolated single umbilical artery. This study is designed to determine the usefulness of routine renal sonography in apparently healthy infants with an isolated single umbilical artery. METHODS: Thirty healthy neonates with a single umbilical artery without a major anomaly from January 1995 to July 2002 were enrolled. The authors investigated the clinical background of babies and their mothers after renal sonography after 72 hours of age. When the abnormalities were found at the first renal sonography, the severity of hydronephrosis and degree of obstruction and renal function were analyzed by follow up renal sonography, voiding cystourethrography(VCUG) and technetium-99m-dimercaptosuccinic acid(DMSA) scan or technetium-99m-mercaptoacetyl-triglycerine (MAG3) scan. RESULTS: Among the 30 healthy patients with isolated single umbilical artery, five patients(16.7%) showed abnormalities on first renal sonography with one major(3.3%) and four(13.4%) minor renal anomaly(minimal or mild hydroneohrosis). One major renal anomaly(severe hydronephrosis) showed severe decreased renal function on MAG3 scan without reflux, and the other four minor regressed spontaneously on follow up study. CONCLUSION: The value of routine early renal sonograpy for detecting renal anomaly in healthy infants with an isolated single umbilical artery remained unclear because most of the anomalies would regress spontaneously in the follow up study.
Follow-Up Studies
;
Humans
;
Hydronephrosis
;
Infant*
;
Infant, Newborn
;
Mass Screening*
;
Mothers
;
Single Umbilical Artery*
6.Two Cases of Severe Neonatal Anemia associated with Massive Feto-Maternal Hemorrhage.
Eun Song SONG ; Ki Hwan KIM ; So Youn KIM ; Chun Hak PARK ; Young Youn CHOI
Korean Journal of Perinatology 2004;15(2):172-173
Feto-maternal hemorrhage, the presence of fetal red blood cells in the maternal circulation, occurs in up to 75% of pregnancies. But its volume is usually very small. Feto-maternal hemorrhage of more than 30 ml of whole blood is relatively rare. The key features that lead to early diagnosis are the maternal history, fetal monitoring, the clinical and laboratory findings of anemia and a negative Coombs' test. Diagnosis is confirmed by Kleihauer-Betke test. Perinatal problems include fetal distress, neonatal anemia, hypovolemic shock, and death. The fetal outcome depends on the amount and rate of bleeding. The initial hemoglobin level was a better predictor than the volume of bleeding. We present two cases of severe neonatal anemia associated with massive feto-maternal hemorrhage, which was confirmed by Kleihauer-Betke test.
Pregnancy
;
Female
;
Infant, Newborn
;
Humans
7.Three cases of nonimmune hydrops fetalis.
Young Youn CHOI ; Jae Sook MA ; Tai Ju HWANG ; Tae Bok SONG
Journal of the Korean Pediatric Society 1991;34(5):691-699
No abstract available.
Ascites
;
Hydrops Fetalis*
;
Pleural Effusion
;
Ultrasonography
8.Two Cases of Multicystic Encephalomalacia in a Surviving Co-twin with One Intrauterine Fetal Death.
Tae Bok SONG ; Young Youn CHOI ; Tae Hyung CHO ; Ji Sun KANG ; Chang Yee CHO
Korean Journal of Perinatology 1998;9(3):314-319
Various anatomical defects have been described in the surviving co-twin who had stillborn, macerated monozygotic co-twin with disseminated intravascular coagulation. The suggested mechanism was the transfer of emboli or thromboplastic materials of dead fetus to co-twin through placental vascular anastomoses. Multicystic encephalomalacia is the condition defined anatomically by the presence of multiple cavities in the great part of both cerebral hemispheres. The most common pathogenesis is circulatory disturbance caused by neonatal asphyxia during the perinatal period. We experienced two cases of monozygotic twin with deceased co-twin at 26 weeks, 33 weeks of gestation and confirmed the diffuse multicystic encephalomalacia by cranial ultrasonography and MRI in a surviving co-twin. Only one patient has been followed who showed spastic cerebral palsy and severe mental retardation. We report two cases of multicystic encephalomalacia in a surviring co-twin with a intrauterine fetal death and its related literatures.
Asphyxia
;
Cerebral Palsy
;
Cerebrum
;
Disseminated Intravascular Coagulation
;
Encephalomalacia*
;
Fetal Death*
;
Fetus
;
Humans
;
Intellectual Disability
;
Magnetic Resonance Imaging
;
Pregnancy
;
Pregnancy, Twin
;
Twins, Monozygotic
;
Ultrasonography
9.Comparison of mRNA expression of gtf genes and adhesive ability of xylitol-sensitive and -resistant Streptococcus mutans by xylitol-treated concentrations.
Sang Uk IM ; Seo Young AN ; Youn Hee CHOI ; Keun Bae SONG
Journal of Korean Academy of Oral Health 2012;36(2):91-96
OBJECTIVES: Xylitol is an effective anticarious natural sugar substitute, by inhibiting the virulence of Streptococcus mutans (S. mutans). However, long-term xylitol consumption leads to an emergence of the xylitol-resistant (XR) strains. This study aimed to confirm the general characteristics, mRNA expression of gtf genes, and adhesive ability of the xylitol-sensitive (XS) and XR S. mutans , by xylitol-treated concentrations. METHODS: S. mutans KCTC3065 was maintained in TYE medium, containing 0.4% glucose with 1% xylitol for 30 days at 37degrees C, 10% CO2 to form XR strain and the same procedures, without xylitol, were repeated for the formation of XS S. mutans. Both XS and XR were cultured by xylitol-treated concentrations (0%, 0.1% and 1%), then, general characteristics, such as growth and acid production, mRNA expression of gtf genes and adhesive ability were analyzed. RESULTS: Xylitol reduced the cell growth of XS S. mutans in a dose-dependent manner, but did not reduce the XR. Xylitol inhibited acid production of XS in a dose-dependent manner. However, it did not inhibit that of XR. Xylitol reduced the gtfB and gtfD mRNA expression of the XS S. mutans, which the genes synthesized soluble and insoluble extracellular polysaccharides, but not reduced that of the XR. By a microtiter plate assay, biofilm formation was more reduced in the XR strains, which means biofilm's adhesive ability of XR S. mutans was lower than that of the XS. CONCLUSIONS: These results indicate that a lower level of adhesive ability for XR S. mutans is related with mRNA expression level of gtf genes, which suggested that the XR strains may be less cariogenic than that of the XS.
Adhesives
;
Biofilms
;
Glucose
;
Glucosyltransferases
;
Polysaccharides
;
RNA, Messenger
;
Sprains and Strains
;
Streptococcus
;
Streptococcus mutans
;
Sweetening Agents
;
Xylitol
10.A Case of Transient Acrodermatitis Enteropathica in a Full-Term Breast-Fed Infant.
Jee Youn WON ; Gi Bong JUNG ; Young Min JEON ; Jee Bum LEE ; Eun Sup SONG
Korean Journal of Dermatology 1999;37(6):790-793
We herein report a case of transient acrodermatitis enteropathica in a 3-month-old, breast-fed, full-term infant. The patient was presented with a 2-month history of diarrhea and crusted erythema- tous patches on the periorificial area. Similar lesions were seen in his siblings. His serum zinc level and the zinc level in his mother's breast milk were markedly reduced. Diarrhea and skin lesions disappeared promptly with oral zinc supplementation and did not recur when zinc was discontinued after three weeks. Our case indicates that even full-term infants, who feed excl-usively on mothers milk, run a risk of developing zinc deficiency, if the concentration of zinc in the breast milk is very low.
Acrodermatitis*
;
Diarrhea
;
Humans
;
Infant*
;
Milk
;
Milk, Human
;
Mothers
;
Siblings
;
Skin
;
Zinc