No abstract available.
Amnion* ; Dinoprostone* ; Humans* ; Metabolism* ; Progesterone*

No abstract available.
Amnion* ; Dinoprostone* ; Humans* ; Metabolism* ; Progesterone*

OBJECTIVES: Our objective was to investigate the effect of coculture system on in vitro fertilization and development of mice embryos. METHODS: F1 hybrid mice were superovulated with PMSG/hCG. Recruited oocytes were divided into three subgroups which are control(subgroup a), Vero cell coculture(subgroup b) and human amniocyte coculture subgroup (subgroup c) respectively. For 3 subgroups, we observed fertilization after 24 hours of incubation. In vitro fertilized early 2-cell stage embryos were allocated to Group I and in vivo fertilized early 2-cell stage embryos were allocated to Group II. Also, each group was divided into control (subgroup a), Vero cell coculture(subgroup b) and human amniocyte coculture subgroup(subgroup c) respectively. For 6 subgroups, we observed in vitro development to blastocyst and that to hatching blastocyst after 120 hours of incubation. RESULTS: As to recruited oocytes, the in vitro fatilization rate of subgroup a was significantly higher than that of subgroup b and subgroup c (P<0.05). In Group I, the developmental rates were not significantly different between the three subgroups. But in Group II, the developmental rates to hatching blastocysts of subgroup b and c were significantly higher than that of subgroup a (p<0.05). CONCLUSION: The development of the in vitro fertilized mouse embryos seemed to be independent of physiologic condition which we think coculture system may give to the embryos. The independent developmental capability of the in vitro fertilized embryos might be obtained through a certain intracellular mechanism for which there should be the need of many more investigations to be verified.
Animals ; Blastocyst ; Coculture Techniques* ; Embryonic Structures* ; Fertilization ; Fertilization in Vitro* ; Humans ; Mice* ; Oocytes* ; Vero Cells

OBJECTIVE: To compare the clinical efficacy of double intrauterine insemination with single intrauterine insemination in GnRH antagonist combined ovarian hyperstimulation (Mild ovarian hyperstimulation) MATERIALS AND METHODS: From Jan. 2001 to Jul. 2004, a retrospective clinical analysis was done of a total of 295 cycles in 170 patients who underwent ovarian hyperstimulation for ART (assisted reproductive technique). Subjects were divided into three groups; only clomiphene citrate ovarian hyperstimulation (n=55, 95cycles), GnRH antagonist combined ovarian hyperstimulation (soft ovarian hyperstimulation) (n=66 99cycles), and GnRH agonist combined ovarian hyperstimulation (short protocol) (n=49, 101cycles) Each group were randomly devided into two subgroups. One group underwent single IUI and the other group underwent double IUI. RESULTS: GnRH antagonist group and GnRH agonist group had similar pregnancy rate. In GnRH antagonist Group, pregnancy rate was 36.1% in single IUI subgroup and was 36.6% in double IUI subgroup. These finding were not statistically significant. And Pregnancy rate was 20.8% in single IUI subgroup and was 19.3% in double IUI subgroup in single clomiphene citrate group, and 36.3% in single IUI subgroup and was 33.3% in double IUI subgroup in GnRH agonist group. These finding were not statistically significant, too. CONCLUSION: Pregnancy rate of GnRH antagonist was high and complication rate such as OHSS and multiple pregnancy was lower. In GnRH antagonist group, to compare with single IUI and double IUI, the result do not statistically differ. So GnRH antagonist single injection with single IUI was relatively comparable ART in infertiliry patient.
Clomiphene ; Female ; Gonadotropin-Releasing Hormone ; Humans ; Infertility* ; Insemination* ; Pregnancy ; Pregnancy Rate ; Pregnancy, Multiple ; Retrospective Studies

No abstract available.
Embryo Transfer* ; Embryonic Structures* ; Fertilization in Vitro*

Heterotopic pregnancy is named when an extrauterine (ectopic) pregnancy coexists with an intrauterine pregnancy simultaneously by many causes such as PID (pelvic inflammatory disease), endometriosis, IUD (intrauterine device), previous pelvic surgery and others. This is very rare in general population, with a range of occurrence estimated between 1:7963 and 1:30000. But recently the incidence has increased as the uses of ARTs (assisted reproductive technologies) including ovulation induction, IVF-ET (in-vitro fertilization and embryo transfer) and GIFT (gamete intrafallopian transfer) increase. Because this has high maternal morbidity, mortality and fetal loss, early diagnosis and proper management is very important. We report a case of heterotopic pregnancy following IVF-ET with a brief review.
Early Diagnosis ; Embryonic Structures ; Endometriosis ; Female ; Fertilization ; Incidence ; Mortality ; Ovulation Induction ; Pregnancy ; Pregnancy, Heterotopic*

No abstract available.
Oocytes* ; Pregnancy* ; Spermatozoa*

Infantile hypertrophic pyloric stenosis(IHPS), which occurs three of 1,000 live births, is a major cause of 1 nonbilious vomiting of early infancy but its etiology and pathogenesis are still obscure. The operation of pyloromyotomy as described by Ramstedt in 1912 remains the standard of care for the treatment of IHPS. From January 1993 to October 1996, 35 infants with IHPS were surgically treated and the following results were obtained. 1. Thirty-five patients comprised 32 males and 3 females, and the ratio of male to female was 10.7:1. 2. The most prevalent age group was between 2 weeks and 8 weeks. 3. Of 35 infants, first born babies were 23 cases(65.7%). 4. Breast feeding was in 23 cases(65.7%). 5. The body weight percentile at admission was lower than 50 percentile in all 35 cases. 11. A total of seven associated anomalies were noted in six patients. 12. All 35 cases were treated with Fredet-Ramstedt pyloromyotomy. 13. There were postoperative complications of wound infection in 2 cases. Intermittent nonprojectile vomiting was presented in 8 cases(22.9%) after operation, but one of them was relieved in 13 days and the rest were relieved within one week by adjustment of oral intake.
Body Weight ; Breast Feeding ; Female ; Humans ; Infant ; Live Birth ; Male ; Postoperative Complications ; Pyloric Stenosis, Hypertrophic* ; Standard of Care ; Vomiting ; Wound Infection

Newborns with ileal atresia frequently present with abdominal distension, bilious vomiting, and failure to pass meconium. Diagnosis is usually established on plain x-ray of the abdomen by the findings of distended small bowel loops and air-fluid levels. In the period of October 1988 to February 1994, 8 patients with congenital ileal atresia were operated and the following results were obtained. 1. Eight patients were comprise of 4 males and 4 females, the ratio of male and female was 1 : 1. 2. Six patients(75%) had been admitted to our hospital during three days of life. 3. Congenital ileal atresia was in 8 cases : Type I in two(25%), Type II in two(25%), Type III a in three(37.5%), Type III b in one(12.5%). 4. There was one premature patient who was small for gestational age. 5. Overall, abdominal distension and bilious vomiting occurring in seven patients, were frequent presenting complaints. 6. Diagnosis was possible with clinical symptom and simple abdomen. 7. Operative treatment was undertaken as soon as the diagnosis was made. In seven cases a primary end-to-end anastomosis was performed after resection of dilated proximal loop. 8. A total of four associated congenital anomalies were found in one patient. 9. Postoperative complications occurred in three cases(37.5%).
Abdomen ; Diagnosis ; Female ; Gestational Age ; Humans ; Infant, Newborn* ; Male ; Meconium ; Postoperative Complications ; Vomiting

No abstract available.
Chorionic Gonadotropin* ; Female ; Gonadotropins* ; Humans* ; Ovulation*