1.Separation of Epidermis from Dermis with a Suction Device in Vitiligo Patients: Light and Electron Microscopic Studies.
Korean Journal of Dermatology 1989;27(5):488-494
Suction is a useful tool for separation of epidermis from dermis in vivo. With appropriate suction pressures and durations applied, cellular damage of the separated epidermis can reduce, This study was performed to examine the appropriste suction pressures and durations in the lhsional and normal skin of four vitiligo patients who wanted to take epidermal autograft. The suction pressures and durations for making the blisters of similar morphology were different according to person. Microscopic changes in the blisters of similar morphology were not very different, and suction of 200 to 250 mmHg for 2 to 3 hours with a 2cm diametered suction cup was considered to be acceptable. Obvious damage to keratinocytes and diminish of melanacyte number were observed with much more powerful and longer suction such as suction of 350 to 400 mmHg for 3 to 4 hours. The level of dermo-epidermal separation was between basal cell membrane.
Autografts
;
Blister
;
Cell Membrane
;
Dermis*
;
Epidermis*
;
Humans
;
Keratinocytes
;
Skin
;
Suction*
;
Vitiligo*
2.Change of Melanocytes in Fixed Drug Eruption.
Korean Journal of Dermatology 1989;27(2):171-176
Lesional and normal skins in 10 patients of fixed drug eruption were studied. Number and vertical distance of rete ridges were measured in H & E stained specimens. Amount of free melanin and number of melanocytes were counted in dopa stained epidermal sheets. According to the number of recurrence, activity and number of melanocytes see med to be changed. The lesions of a few recurrence showed only active melano cytes, however, those of frequent recurrenoe increased number of melanocytes with marked elongation of rete ridges. It is supposed that the numerical increase of melanocytes were from the elongation of rete redges.
Dihydroxyphenylalanine
;
Drug Eruptions*
;
Humans
;
Melanins
;
Melanocytes*
;
Recurrence
;
Skin
3.Ultrastructural study of fixed drug eruption.
Korean Journal of Dermatology 1991;29(4):466-470
No abstract available.
Drug Eruptions*
4.Effect of suction blister fluid and IFN-gamma on HLA-A,B,C and HLA-DR espression in cultures human melanocytes.
Korean Journal of Dermatology 1991;29(2):151-154
HLA-A, B, C and HLA-DR are very important in the regulation of immune response and of transplant success or failure, but there have been a few reports about F3I A expression in melanocyt.es, a minor population of cpidermal cells. In the treatment of stable or segmental vitiligo, allogeneic melanocytes graft could t)e potentially useful if rejection does not. occur. The aim of this study was to examine the expression of HI.A-A, B, C and HI.A-DR in cultured hurnan melanocytes with and without. stimulation by INF-r and suction blister fliid. All strains of cultured me- lanocytes expressed HLA-A, B, C regardless of stmulation, but HLA-DR was expressed only following stimulation of INI-r. HLA expression in me]anocytes appears to be similar to that in keratinocytes.
Blister*
;
HLA-A Antigens
;
HLA-DR Antigens*
;
Humans*
;
Keratinocytes
;
Melanocytes*
;
Suction*
;
Transplants
;
Vitiligo
5.A Case of Allergic Contact Dermatitis to Cenasert® Vaginal Suppository.
Young Gull KIM ; Ai Young LEE ; Yoo Shin LEE
Annals of Dermatology 1991;3(2):130-132
Sensitization to Cenasert®, a broad spectrum antimicrobial for the treatment of leukorrhea, is rare. Perineal contact dermatitis due to vaginal suppositories has never been reported in the medical literature. We report a 31-year-old woman, who developed perineal skin eruptions following the treatment of leukorrhea with Cenasert® vaginal suppositories. Patch tests showed positive reactions to Cenasert® suppositories and to the three active ingredients of Cenasert®. Vaginal suppositories can sensitize not only the vagina but also the surrounding perineum due to leakage of the allergen from the vaginal vault.
Adult
;
Dermatitis, Allergic Contact*
;
Dermatitis, Contact
;
Female
;
Humans
;
Leukorrhea
;
Patch Tests
;
Perineum
;
Skin
;
Suppositories*
;
Vagina
6.Pseudoxanthoma Elasticum.
Sung Young JOHN ; Yoo Shin LEE
Korean Journal of Dermatology 1970;8(1):77-80
Two cases of pseudoxanthoma elasticum associated with angioid streaks in both eyes were presented and the literatures were reviewed. The affected sisters were 15 and 18 years female among the five siblings. They had pin head sized yellowish maculopapular eruptions on the sides of the neck and axillae, and also visual distubance of the both eyes. They seemed to be autosomal recessive inberitance in their family background. There were no other complications.
Angioid Streaks
;
Axilla
;
Female
;
Head
;
Humans
;
Neck
;
Pseudoxanthoma Elasticum*
;
Siblings
7.T cell phenotype and intracellular IFN-gamma production in peritoneal exudate cells and gut intraepithelial lymphocytes during acute Toxoplasma gondii infection in mice.
The Korean Journal of Parasitology 2002;40(3):119-129
Although there are many reports on the splenic (systemic) T cell response after Toxoplasma gondii infection, little information is available regarding the local T cell responses of peritoneal exudate cells (PEC) and gut intraepithelial lymphocytes (IEL) following peroral infection with bradyzoites. Mice were infected with 40 cysts of the 76K strain of T. gondii, and then sacrificed at days 0, 1, 4, 7 and 10 postinfection (PI). The cellular composition and T cell responses of PEC and IEL were analyzed. The total number of PEC and IEL per mouse increased after infection, but the ratio of increase was higher in IEL. Lymphocytes were the major component of both PEC and IEL. The relative percentages of PEC macrophages and neutrophils/eosinophils increased significantly at day 1 and 4 PI, whereas those of IEL did not change significantly. The percentage of PEC NK1.1 and gamma delta T cells peaked at day 4 PI (p < 0.0001), and CD4 and CD8 alpha T cells increased continuously after infection. The percentages of IEL CD8 alpha and gamma delta T cells decreased slightly at first, and then increased. CD4 and NK1.1 T cells of IEL did not change significantly after infection. IFN-gamma-producing PEC NK1.1 T cells increased significantly from day 1 PI, but the other T cell subsets produced IFN-gamma abundantly thereafter. The proportion of IEL IFN-gamma-producing CD8 alpha and gamma delta T cells increased significantly after infection, while IEL NK1.1 T cells had similar IFN-gamma production patterns. Taken together, CD4 T cells were the major phenotype and the important IFN-gamma-producing T cell subsets in PEC after oral infection with T. gondii, whereas CD8 alpha T cells had these roles in IEL. These results suggest that PEC and IEL comprise different cell differentials and T cell responses, and according to infection route these factors may contribute to the different cellular immune responses.
Acute Disease
;
Animals
;
Ascitic Fluid/cytology/*metabolism
;
Female
;
Interferon Type II/*biosynthesis
;
Intestinal Mucosa/cytology
;
Lymphocytes/*metabolism
;
Mice
;
Mice, Inbred C57BL
;
Support, Non-U.S. Gov't
;
T-Lymphocyte Subsets/*immunology
;
Toxoplasmosis/*immunology
8.Measurement of glomerular filtration rate with radionuclide plasma clearence method.
Kang Wook LEE ; Young Tai SHIN
Korean Journal of Nephrology 1993;12(1):36-43
No abstract available.
Glomerular Filtration Rate*
;
Plasma*
9.Neurilemmoma: A Dome-lesion on the Palm.
Korean Journal of Dermatology 1998;36(6):1101-1102
We report a solitary neurilemmoma on the left palm that occured in a 19-year-old man. The mass was a 1 * 1.2 * 0.7cm sized dome which was located between the thenar and hypothenar on the left palm. A biopsy specimen showed histological findings consistent with neurilemmoma:circumscribed nuclear palisading with Verocay bodies.
Biopsy
;
Humans
;
Neurilemmoma*
;
Young Adult
10.The management of costochondritis of the chest wall.
Young Jin SHIN ; Taik Jong LEE
Journal of the Korean Society of Plastic and Reconstructive Surgeons 1992;19(1):67-72
No abstract available.
Thoracic Wall*
;
Thorax*