1.Family Stress, Perceived Social Support, and Coping in Family who has a Developmentally Disabled Child.
Korean Journal of Child Health Nursing 1997;3(1):42-51
Although a family-centered approach to health care for developmentally disabled children has been advocated, existing systems of care have not adequately addressed the support needs of the family system and the essential role that parents play in the daily care of these children. The overall purpose of this research is to examine family system adaptation to the care of a developmentally disabled child using the Resiliency Model of Family Stress, Adjustment, and Adaptation Framework. Relationships among family system demands(stressors, strains, transitions, child's illness factors) and family system strengths and capabilities(resource of social support) were examined to determine their separate and combined contribution to explaining the variability inn family system outcomes(family system coping). The subject for this study was 46 families who have a child with developmental disabilities(mental retardation and/or autism) from three special educational programs in Seoul, Korea, Results from correlation and hierachial regression analysis revealed that perceived social support operated as a resiliency factor between family stress and coping. Child and family characteristics appeared to be important predictors of perceived social support and coping. In summary, there is evidence that the resource of social support as a family strength and capability was found to improve the family coping. These findings also must be viewed within the context that sample of families of children with disabilities was relatively small and eligible families from support group of special educational program.
Child
;
Delivery of Health Care
;
Disabled Children*
;
Family Characteristics
;
Humans
;
Korea
;
Parents
;
Self-Help Groups
;
Seoul
;
Child Health
2.Immunoelectrophoresis for Fasciola hepatica.
The Korean Journal of Parasitology 1979;17(1):73-80
In an attempt to investigate the specific antigenic substance of Fasciola hepatica, Ouchterlony tests and immunoelectrophoretic analyses were carried out. Crude Fasciola antigen was prepared and fractionated by Sephadex G-200 column to Antigen I, II and III according to protein content. Crude antigens of Paragonimus westermani, Clonorchis sinensis and Paramphistomum sp. were also prepared for control and absorption study. Antiserum was prepared by injecting 0.5 ml of crude Fasciola antigen with same amount of complete Freund's adjuvant in rabbits, 10 times at an interval of l week. The results obtained in this study were as follows: Crude Fasciola antigen reacted with antiserum with 9 precipitin bands by Ouchterlony test and with 11 bands by immunoelectrophoresis. Cross reaction was observed between Paragonimus, Clonorchis and Paramphistomum antigens and anti-Fasciola rabbit serum respectively. By Ouchterlony test, 3-4 cross reacting bands were found. Anti-Fasciola sera which were absorbed with respective Paragonimus, Clonorchis and Paramphistomum antigens, reacted with Fasciola crude antigen. Ouchterlony test gave 5-6 precipitin bands. Further reaction between Fasciola antigen and antiserum absorbed with the above 3 antigens concomitantly gave 5 precipitin bands by Ouchterlony test and 7 bands by immunoelectrophoretic analyses. Fractionated Fasciola antigens (Antigens I, II and III) reacted with anti-Fasciola rabbit serum in immunoelectrophoresis. Antigen I, II and III gave 2, 3 and 5 precipitin bands respectively. Anti-Fasciola rabbit serum which was absorbed with 3 trematodes antigens gave, by immunoelectrophoresis, 1 band with Antigen I, 2 bands with Antigen II and III of Fasciola hepatica. From the above results, it is concluded that Fasciola hepatica possessed the specific antigenic substance not cross-reacted with other trematodes.
parasitology-helminth-trematoda
;
Fasciola hepatica
;
Paragonimus westermani
;
Clonorchis sinensis
;
Paramphistomum sp.
;
antigen
;
immunology
;
electrophoresis
3.Serological studies on rabbits inoculated with Toxoplasma gondii.
The Korean Journal of Parasitology 1975;13(1):7-18
Serological studies on toxoplasmosis were conducted with rabbits sera that were immunized with RH strain or infected with Beverley strain of Toxoplasma gondii. Complement fixation tests, agar-gel double diffusion tests and agar-gel immunoelectrophoresis were performed. Toxoplasma crude antigen was prepared from the organisms in mice peritoneal fluids, which were infected with RH strain of Toxoplasma gondii. The organisms were suspended in saline volume originally exudated and counted in hemocytometer for purity of the organisms over 99 per cent. These suspended organisms were prepared by sonication, and the solution was centrifuged for 30 min. at 10,000 rpm in 4C. These supernatant fluids were used as crude antigen. On the other hand, purified antigens were fractionated on Sephadex G-200 gel filtration. A Sephadex G-200 column, 80 by 4 cm, equilibrated with Tris-HCl-(0.1 M)-NaCl (1.0 M) buffer, pH 8.0 was used. The eluate fractions were collected in 3 ml per hour and the absorbance at 280 nm was measured with a Beckman Du-2 spectrophotometer. Each tube is pooled into 6 fractions by protein density graph. For immunization of rabbits, crude antigen of RH strain was emulsified with an equal amount of incomplete Freund's adjuvant and l ml of mixture was injected subcutaneously into them once a week for 5 successive weeks. Antisera were obtained at an interval of a week, beginning the first week after the last immunization, while several rabbits were infected with Beverley strain of Toxoplasma gondii by inoculating about 200 cysts and antisera were obtained from them serially at a week interval. The results were as follows: The sera from the rabbits immunized with the RH strain or infected with Beverley strain of Toxoplasma gondii againist the crude antigen showed the first positive reactions in 2 or 3 weeks after the administration or immunization in complement fixation tests. Maximum titers appeared in 4 or 5 weeks after immunization with RH strain and in 7 or 9 weeks after infection with Beverley strain respectively. Complement fixation tests showed the positive reactions in the rabbits sera immunized with RH strain against the purified antigens II, III, IV, V and VI: moreover, antigen IV fraction showed the highest titer. On the other hand in the rabbits sera infected with Beverley strain against the purifed antigens II, III and IV fractions showed the positive reaction; especially, antigen fraction IV showed the highest titer. In immuno-diffusion tests, the sera from the rabbits immunized with RH strain and infected with Beverley strain, against the crude antigen appeared the precipitin bands 2 weeks after the immunization or infection. And the former showed the 2 or 5 precipitin bands after 5-8 weeks and the latter showed the l or 2 precipitin bands after 6 weeks. The sera from the rabbits immunized with RH strain against the purified antigens II, III, IV,V and VI showed the precipitin bands, and the sera from the rabbits infected with Beverley strain against the purified antigens II, III and IV showed the precipitin bands in the immuno-diffusion tests. Especially antigen IV was the strongest reaction against the sera from RH strain and Beverley strain. In agar-gel immunoelectrophoresis, the immunized sera against the crude antigen showed 8 arcs. But the infected sera against the crude antigen showed 4 or 5 arcs. The immunized sera against the fractionated antigens II, III, IV, V, VI showed arcs, but against the fractionated antigen IV showed 6 arcs and in the antigens II, III, V, VI showed l or 2 arcs only. On the other hand, the infected sera against the fractionated antigens IV showed 4 arcs, II and III showed the l arcs, which was the most weak of all.
parasitology-protozoa-Toxoplasma gondii
;
toxoplasmosis
;
rabbit
;
immunology
;
electrophoresis
4.Immunoelectrophoretic studies of several helminths.
The Korean Journal of Parasitology 1979;17(2):147-153
In an attempt to investigate the antigen-antibody relations and the value of immunodiagnosis for several helminths, Ouchterlony tests and immunoelectrophoreses were carried out. Taenia saginata, Cysticercus sp. of cestodes, Clonorchis sinensis, Fasciola hepatica and Paramphistomum sp. of trematodes,and Ascaris suum of nematodes were used as antigens. On the other hand, antisera were obtained by injecting 0.5 ml each of the above antigens and the same amount of complete Freund's adjuvant into rabbits ten times at an interval of one week. The result obtained in this study are as follows: A larger number of precipitin arcs were demonstrated in homologous antigen-antibody reactions than in heterologous antigen-antibody reactions both in Ouchterlony tests and immunoelectrophoreses. Gross reactions were observed between the different species of the same class, but no cross reactions were noticed when the classes were different with one or two exceptions, such as between T. saginata, F. hepatica and A. suum. In A. suum, the difference between the male and female was more distinct in Ouchterlony test and immunoelectrophoresis than in the examination of organs such as genital organ and coeliac fluid. Immunoelectrophoresis revealed specific arcs and higher sensitive reaction than Ouchterlony test, and was considered to be a more valuable method for identifing species and immunological diagnosis.
parasitology-helminth-trematoda
;
cestoda
;
nematoda
;
immunoelectrophoresis
;
Taenia saginata
;
Cysticercus
;
Clonorchis sinensis
;
Fasciola hepatica
;
Paramphistomum sp.
;
Ascaris suum
;
antigen
;
immunology
5.Agar-gel precipitin reactions in experimental paragonimiasis.
The Korean Journal of Parasitology 1981;19(2):101-108
In an attempt to investigate the sensitivity of immunodiagnosis in cats experimentally infected with Paragonimus westermani, agar-gel precipitin reaction were studied. Metacercariae of P. westermani were administered to cats in various doses(2-100 metacercariae per cat) and antisera were obtained at an interval of a week. Precipitin bands appeared in homologous antigen-antibody in experimental paragonimiasis between 3 and 5 weeks after infection in all the cats. Almost all the cases in which a large number of worms were detected, showed strong reactions as revealed by deeply stained bands. Precipitin reactions did not necessarily parallel with the number of worms detected. This may be attributable to the individual difference of a cat's conditions. Very weak precipitin reactions were noticed between Clonorchis antigen and Paragonimus antisera of cats, but no reactions were noticed between Paragonimus antigen and Clonorchis antisera of cats or rabbits.
parasitology-helminth-trematoda
;
paragonimiasis
;
Paragonimus westermani
;
immunology
;
Clonorchis sinensis
;
cat
;
rabbit
6.Determinants of health behavior in highschool students.
Hee Young SO ; Young Ran LEE ; Sig Young SON
Journal of Korean Academy of Adult Nursing 1997;9(1):44-54
The purpose of this study was to test Interaction Model of Client Health Behavior(IMCHB) of Cox repeatedly and to determine the contributions of sociodemographic variables, health experiences, family functioning, intrinsic motivation, health perception, self esteem, to health behavior of highschool students. A convenient sample of 2nd grade highschool students(N=638) responded to self-report questionnaires. The IMCHB was then tested with data using SAS program by frequency, t-test, ANOVA, regression. The results were as follows 1. Health behaviors were found to be gender specific. 2. From the body's model, mother and students health affected health perception and self-esteem among a set of background variables. Health perception and self-esteem are related to health behaviors. 20% of the varience in body's health behavior are explained by health perception and self-esteem. 3. From the girls model, students health explained 13.3% of health perception varience and father's health explained 11.6% of self-esteem varience. 16% of health behavior varience are explained by health perception and self-esteem. The results indicated that there was a relatively poor fit of the IMCHB to the highschool students data. But further study using the IMCHB to define health outcome in student is needed to verify the linkage. The model generated in this study clarified the contribution of some factors such as parental and student's health status. The results also indicated that the schoolnurse has to develop the health care program for students and their parent with community resources to improve health perception and self-esteem of students for intervening health behavior of highschool students.
Delivery of Health Care
;
Female
;
Health Behavior*
;
Humans
;
Mothers
;
Motivation
;
Parents
;
Self Concept
;
Surveys and Questionnaires
7.Indirect fluorescent antibody test for diagnosis of paragonimiasis.
Won Young CHOI ; Won Koo LEE ; Ok Ran LEE
The Korean Journal of Parasitology 1975;13(2):152-158
Indirect fluorescent antibody tests were performed with sera of paragonimiasis patients and skin test positive sera against Paragonimus antigen. Paragonimus antigen was prepared from lyophilized adult worms of P. westermani by defatting with ethyl-ether before extracting with barbital buffered saline. Preparation of Paragonimus antigen for the indirect fluorescent antibody test was based upon Sato's method used for sero-diagnosis of anisakiasis, with Sephadex G-25 instead of Sepharose 4B. The results were as follows: The indirect fluorescent antibody titers of paragonimiasis patient's sera ranged from 1:64 to 1:512, whereas the control sera showed titers of less than 1:16. As controls, Clonorchis patient's sera and parasite-free healthy human sera were used. In indirect fluorescent antibody tests, the skin test positive human sera against Paragonimus antigen showed a positive rate of 41.5 per cent in the case of titers more than 1:40. On the other hand, complement fixation tests on the same sera showed a positive rate of 32.5 per cent in the case of titers more than 1:20.
parasitology-helminth-trematoda
;
paragonimiasis
;
Paragonimus westermani
;
diagnosis
;
indirect fluorescent antibody tests
;
serum
;
ethyl-ether
8.The experiments on the infectivity to mice of the Ascaris eggs irradiated with Cobalt(60).
Ok Ran LEE ; Baek Hyun YUN ; Won Young CHOI
The Korean Journal of Parasitology 1970;8(3):90-95
In order to find out a valuable control measure for soil-transmitted parasties, the infectivity in mice of Ascaris eggs irradiated with Cobalt(60) were examined. The results were summarised as follows. In vitro, Ascaris eggs irradiated with larger doses of Cobalt(60) developed poorly, and no difference was found between fresh eggs and those cultured for a few days. Ascaris eggs irradiated with doses of 200,000 rad. developed at the rate of 90 percent after 4 weeks, whereas those irradiated with 1,000,000 rad. developed 28 percent. Ascaris eggs irradiated with Cobalt(60) after 2 weeks of culture were poor in development compared with those of 4 week culture. Eggs cultured for 5 weeks showed weaker infectivity in mice than those cultured for 8 weeks. In the control groups, the infectivity in mice of Ascaris eggs was remained the same between 5 and 8 weeks. The minimum dose of Cobalt(60) irradiation effective for preventing infectivity in mice was estimated to be 200,000 rad.
parasiotology
;
radiology
;
prevention
;
Ascaris suum
;
nematode
;
Cobalt(60)
;
irradiation
9.A Case of Cutaneous Epithelioid Angiosarcoma.
Mee Ran LEE ; Young Ho WON ; Inn Ki CHUN
Korean Journal of Dermatology 1994;32(1):177-181
The epithelioid angiosarcoma is recently described ns a variant of ang-osarcoma based on its pathological feature, which is characterized by the epithelioid or histiocytoid morphology af the malignant tumor cells. The existence of vascular endothelial tumor with an epithelioid feature has been accepted for many years, most notablyn the forms of epithelioid hemangioma and epithelioid hemangioendo thelioma. In recent years, cutaneous angiosarcoma with epitheloid morphology have been reported as a cutaneos epithelioid angiosarcoma in the literatures. A 60-year-old man presente with a mild tender erythematous 2 x 2 x 2.5cm sized soft fluctuating single nodule with dark hemorrhagic crust on the left forehead for 3 months. He had had an abrasion wound on the lesion site of the forehead by an accidental trauma 5 month before the lesion appeared. Histopathological findings revealed that the tumor mass was chiefly composed of solid sheets of poorly differentiated malignant cells with an epithelioid feature. The characteristics of classical angiosarcoma such as primitive vascular spaces and clefts with malignat cells, and proliferating vessels were also found in some areas. The immunohistochemical stain wi.h the factor VIII related antigen ivas focally reactive in the tumor cells. Unfortunately he refused further treatment and expired 7 months after discharge at home without knowing the direct cause of his health. We report herein an interesting and rare case of the cutaneous epitheioid angiosarcoma which had typical clinical and histopathological findings and suggested as a case with a very aggressive course.
Forehead
;
Hemangioma
;
Hemangiosarcoma*
;
Humans
;
Middle Aged
;
von Willebrand Factor
;
Wounds and Injuries
10.The Effect of Dance Therapy on Physical and Psychological Characteristics in The Elderly.
Journal of Korean Academy of Nursing 1999;29(2):429-444
This study was performed to explore the effects of a dance therapy on physical and psychological characteristics in the elderly. The design of this study was a non-equivalent pre-post test experiment. The subjects consisted of elderly persons living in a facility located in Suweon and Bucheon. Fifty eight subjects, aged between 65 and 93 years who had normal cognition, sensory function, balance, and resting blood pressure. They underwent tests of balance, flexibility, muscle strength, depression, and anxiety as baseline data before dance therapy, and at 6 th week and at the end of the 12nd week after following dance therapy. Twenty seven elderly persons were assigned to the experimental group and participated with the dance therapy between April and July, 1998. The dance therapy was developed by the author with the help of a dance therapist and a physiatrist. This therapy was based on the Marian Chace's dance therapy and Korean traditional dance with music. The dance therapy was developed by the author with the help of a dance therapist and a physiatrist. This therapy was based on the Marian Chace's dance therapy and Korean traditional dance with music. The dance therapy consists of 50 minutes session, 3 times a week for 12 weeks. One session was consisted of warming-up, expression, catharsis, sharing, and closing stage. The intensity of the dance therapy was at the 40% of age-adjusted maximum heart rates. Data were analyzed with mean, standard deviation, Chi-square test, unpaired t-test, repeated measures ANOVA, and Bonferroni multiple regression using SAS program. 1. The results related to the physical characteristics were as follows : 1) The balance (standing on one leg, walking on the balancing bar), flexibility and muscle strength (knee extensor, knee flexor, ankle plantarflexor and dorsiflexor) of the experimental subjects significantly increased over time more than that of the control subjects. 2) The experimental group had significantly higher score for balance, flexibility, muscle strength of knee extensor, and knee flexor than the control group at the 12nd week after dance therapy. 3) The experimental group had significantly higher score for muscle strength of ankle dorsiflexor and plantarflexor than the control group at the 6th week and the 12nd week after dance therapy. 2. The results related to psychological characteristics were as follows : 1) Scores of Geriatric Depression Scale, Hamilton Depression Rating Scale, and Zung's Self-rating Anxiety Scale of the experimental group were significantly decreased over time more than that of the control group. 2) The experimental group had significantly lower score for depression than the control group at the 12nd week after dance therapy. 3) The experimental group had significantly lower score for anxiety than the control group at eh 6th week and the 12nd week after dance therapy. The findings showed that the dance therapy could be effective in improving the balances, flexibility, and muscle strength of lower limb, and effective in decreasing the depression and anxiety of the elderly. Additional merits of the dance therapy would be inexpensiveness, easy accessibility, and increasing interpersonal relationship. It can be suggested that the dance therapy is effective in the health promotion of the elderly.
Aged*
;
Ankle
;
Anxiety
;
Blood Pressure
;
Catharsis
;
Cognition
;
Dance Therapy*
;
Depression
;
Evaluation Studies as Topic
;
Gyeonggi-do
;
Health Promotion
;
Heart Rate
;
Humans
;
Knee
;
Leg
;
Lower Extremity
;
Muscle Strength
;
Music
;
Pliability
;
Sensation
;
Walking