No abstract available.

No abstract available.
Intracranial Arteriovenous Malformations* ; Risk Factors*

Purpose: This study is a descriptive research study to understand the effect of compassion competence, clinical nursing character, and nursing professionalism on nursing service quality provided by long-term care hospital nurses. Methods: The participants were 187 nurses working in six long-term care hospitals. Data were collected from October 30 to December 5, 2023, and analyzed using the SPSS/WIN 26.0 program (descriptive statistics, one-way ANOVA, independent t-test, Scheffé test, Pearson’s correlation coefficients, and Multiple regression analysis). Results: As a result of this study, the nursing service quality of nursing hospital nurses was positively correlated with compassion competence (r=.59, p<.001), clinical nursing character (r=.67, p<.001), and nursing professionalism (r=.52, pp<.001). The factors affecting nursing service quality were clinical nursing character, nursing professionalism, and compassion competence. Also, the explanatory power of the model was 52.3% (F=69.09, p<.001, Adj R2 =.52). Conclusion The result of this study is meaningful in that they identified the factors affecting the quality of nursing service of long-term care hospital nurses and provided basic data for improving the quality of nursing services.

No abstract available.
Aneurysm* ; Histiocytoma, Benign Fibrous*

No abstract available.
Facial Nerve* ; Magnetic Resonance Imaging* ; Paralysis*

The ultrastructural changes of mitochondria in the ovarian oocytes from Graafian follicles, the ovulated tubal ova, and the various stages of preimplantation rabbit embryos have been observed with an electron microscope. From the ovarian oocytes to the 4-cell stage, mitochondria showed oval and round forms with a few cristae arranged concentrically and peripherally at the inner membrane. In 8-cell and 16-cell stages, mitochondria tended to change their forms to be elongated, and their sizes, and the outer membrane of the mitochondria had a tendency to become rough and irregular although there were few changes in the inner structure. In morula, some mitochondria began to show several transverse cristae proceeding into the matrix. Mitochondria rapidly increased in number at the late blastocyst stage. Matrix of mitochondria with transverse cristae found in the morula and in blastocyst stages was less dense than that of the earlier stages. The authors believe that the morphological changes of mitochondria during early embryonal development indicate the level of enzymatic activity at which this organelle is engaged in energy metabolism.
Animal ; Cell Membrane/ultrastructure ; Embryo/ultrastructure* ; Embryo Implantation ; Female ; Microscopy, Electron ; Mitochondria/ultrastructure* ; Organoids/ultrastructure ; Ovum/ultrastructure ; Rabbits

PURPOSE: To investigate the effect of combined inhibition of protein kinase B (AKT) and SRC on the growth and metastatic potential of human pancreatic cancer cells. MATERIALS AND METHODS: AKT and SRC were inhibited using 10-DEBC and PP2, respectively. The expression of their messenger RNAs were down-regulated by specific small interfering RNA (siRNA). Changes in pancreatic cancer cell growth and metastatic potential were determined using a cell viability assay and a xenotransplant model of pancreatic cancer, as well as cell migration and invasion assays. Signal proteins were analyzed by Western blot. RESULTS: The inhibitors 10-DEBC and PP2 suppressed cell proliferation in a dose-dependent fashion in pancreatic cancer cell lines MIA PaCa-2 and PANC-1. The simultaneous inhibition of AKT and SRC at low concentrations resulted in a significant suppression of cell proliferation. Knockdown of AKT2 and SRC using siRNAs also significantly decreased cell proliferation. In a pancreatic cancer model, combined treatment with 10-DEBC and PP2 also significantly suppressed the growth of pancreatic cancer. Application of 10-DEBC with PP2 significantly reduced the metastatic potential of pancreatic cancer cells by inhibiting migration and invasion. The combined inhibition suppressed the phosphorylation of mTOR and ERK in pancreatic cancer cells. CONCLUSION: Combined targeting of AKT and SRC resulted in a synergistic efficacy against human pancreatic cancer growth and metastasis.
Blotting, Western ; Cell Line ; Cell Movement ; Cell Proliferation ; Cell Survival ; Humans* ; Neoplasm Metastasis ; Pancreatic Neoplasms* ; Phosphorylation ; Proto-Oncogene Proteins c-akt ; RNA, Messenger ; RNA, Small Interfering

The present experiments were conducted to evaluate the effects of follicular fluid on maturation of bovine follicular oocytes. TC medium 199 seemed to be in adequate for this purpose since a high proportion (ranging 84.1. to 97.0%) of the oocytes were able to resume meiotic division in both media-with or without the addition of follicular fluid. This implies a possible similarity between TC medium 199 and follicular fluid with regard to the components initiating meiosis. Actually, TC medium 199 contains amino acids, vitamins and carbohydrates most of which are also found in follicular fluid. For this reason, the effect of follicular fluid on the ovum maturation was investigated by applying Krebs-Ringer bicarbonate solution (SECM), which was main1y composed of the salts, pyruvate and lactate. When the oocytes were cultured in SECM without the addition of follicular fluid, only 7-14% of them resumed meiotic division within 30 hours. However, when follicular fluid was added, the proportion of oocytes undergoing maturation was sharply increased to about 70%. Among the groups cultured in media containing different concentrations of follicular fluid, the proportion of the oocytes in meiosis remained constant, In pure follicular fluid, 87-89% of the oocytes showed enhancement of meiotic division. The presence of the follicular fluid contributed to a decrease in the production of degenerative oocytes. As a consequence it has been noted that addition of follicular fluid to the culture medium provides a more beneficial environment for cow oocytes. Meiotic division is initiated and production of degenerative oocytes is inhibited.
Animal ; Cattle ; Cells, Cultured ; Culture Media ; Female ; Meiosis ; Oocytes/physiology* ; Ovarian Follicle/physiology* ; Ovum/physiology*

PURPOSE: To describe the MR findings for the three phases of eosinophilic granuloma, as defined by Mirra 'sconventional radiographic criteria. MATERIALS AND METHODS: Eighteen lesions in 14 patients with proveneosinophilic granuloma were retrospectively analyzed. Among this total, three vertebral lesions were excluded,and the remaining is were classified as early, middle, or late phase on the basis of Mirra's radiographiccriteria. For each phase, we compared MR findings with regard to signal intensity, homogeneity, contrastenhancement, perilesional marrow edema, and soft tissue change. For the three vertebral lesions excluded becausethe application of radiographic criteria was difficult, MR findings for paravertebral soft tissue reaction anddegree of cord compression were compared. RESULTS: Of the fifteen cases classified, eight were early phase, fivewere mid phase, and two were late phase. During each phase, all lesions except one, as seen on T1-weightedimages(T1W1), showed iso-signal intensity. On T2WI, all lesions showed high signal intensity. Contrast studydemonstrated marked contrast enhancement. Thus, no remarkable differences were found in the signal intensitydegree of contrast enhancement of each phase. With regard to heterogeneity, this was demonstrated in most earlyphase lesions, reflecting necrosis and hemorrhage of those lesions. Soft tissue swelling was more severe duringthe early phase than the mid or late phase, but marrow edema was similar in each of the three phase. One of threepatients with vertebra plana showed para-vertebral soft tissue swelling and cord compression, but this was notseen in the two other cases. CONCLUSION: For evalvating the extent of eosinophilic granuloma and its relationshipwith surrounding structures, MRI was superior to conventional radiography. During the early phase of the disease,lesions showed greater inhomogeneity and more aggressive soft tissue reaction than during the mid and late phase.The use of MRI for the evalvation of eosinophilic granuloma can help decide a therapeutic plan of action andfollow up evaluation.
Bone Marrow ; Edema ; Eosinophilic Granuloma* ; Eosinophils* ; Granuloma ; Hemorrhage ; Histiocytosis ; Humans ; Magnetic Resonance Imaging ; Necrosis ; Population Characteristics ; Radiography ; Retrospective Studies ; Spine

BACKGROUND AND OBJECTIVES: Transient evoked otoacoustic emission (TEOAE) has an increasingly signficant role in pediatric otological practice such as screening hearing in newborn babies. To assess the potential applicability of the TEOAE measurements on the sequential monitoring of pediatric patients with middle ear effusion (MEE), we investigated the effects of MEE on the TEOAE responses. MATERIALS AND METHODS: TEOAE responses were recorded from 33 patients (56 ears) before and after ventilation tube insertion. RESULT: In the 37 cases with MEE, we found that the average band reproducibility below 2 kHz was recovered significantly after the tube placement, although the band reproducibility at 5 kHz was diminished. In the 19 cases without MEE, no significant changes in echo amplitude and band reproducibility were noted in the postoperative TEOAE measurements compared to the preoperative measurements. CONCLUSION: The results of this study suggest that an improvement in echo response and band reproducibility less than 2 kHz in serial TEOAE measurements may indicate the resolution of MEE in children with OME.
Child ; Hearing ; Humans ; Infant, Newborn ; Mass Screening ; Otitis Media with Effusion* ; Otitis Media* ; Otitis* ; Ventilation