1.Development of diagnostic method of helicobacter pylori infection: I. molecular cloning and DNA sequencing of urease.
Cheol Keun PARK ; Woo Kon LEE ; Young Mi DOH ; Myung Je CHO ; Kwang Ho RHEE
Journal of the Korean Society for Microbiology 1991;26(6):541-552
No abstract available.
Cloning, Molecular*
;
DNA*
;
Helicobacter pylori*
;
Helicobacter*
;
Sequence Analysis, DNA*
;
Urease*
2.A case of Glanzmann's thrombasthenia diagnosed with flow cytometry and SDS-PAGE analysis of platelet membrane glycoprotein.
Yoon Jeong DOH ; Mi Hyang KIM ; Chung Hyun NAHM ; Kyung Soon SONG ; Oh HunK WON ; Kir Young KIM
Korean Journal of Hematology 1992;27(2):443-451
No abstract available.
Blood Platelets*
;
Electrophoresis, Polyacrylamide Gel*
;
Flow Cytometry*
;
Membrane Glycoproteins*
;
Membranes*
;
Thrombasthenia*
3.Cervical tuberculous lymphadenitis : Clinicopathological reatures and AFB positivity.
Young Jun HWANG ; Mi Hye KO ; Se Young YUN ; Yong Ho KIM ; Doh Hyung KIM ; Kye Young LEE ; Keun Youl KIM ; Na Hye MYONG ; Jae Seuk PARK
Tuberculosis and Respiratory Diseases 2000;48(5):720-729
BACKGROUND: Histological analysis of tuberculosis shows a spectrum of findings, from well formed granulmatous inflammation with few bacilli in patient with normal immune response to M. tuberculosis to poorly formed granulomatous inflammation with many bacilli in patient with defective immune response. To evaluate the degree of immune response to M. tuberculosis, we studied the histologic features, including the presence of acid fast bacilli(AFB) in lymph node of patients with cervical tuberculous lymphadenitis, and compared them with clinical characteristics. METHODS: We reviewed the histologic features of 33 cases of cervical tuberculous lymphadenitis and processed the excised nodes for auramine-rhodamine staining to detect AFB. The AFB positivity in tissue was compared with the histologic features(degree of granuloma formation, presence of caseation necrosis, presence of neutrophilic infiltration) and clinical characteristics (lymph node size, duration of symptom, presence of local symptom or radiologic evidence of pulmonary tuberculosis). RESULTS: 1) The mean age at diagnosis was 42.4 years, and male to female ratio was 1 : 45. 2) Histologically, all cases showed well formed granuloma and variable degrees of caseation necrosis, and 39% of the cases showed neutrophilic infiltration in the granulomatous inflammation. 3) AFB were confirmed in 52% of the cases, and they were found extracellularly and at the periphery of caseation necrosis. 4) There was no association between AFB Positivity and histological features or clinical characteristics. CONCLUSION: Cervical tuberculous lymphadenitis showed well formed granulomatous inflammation with caseation necrosis, and there was no association between AFB positivity in the tissue and histological or clinical characteristics.
Diagnosis
;
Female
;
Granuloma
;
Humans
;
Inflammation
;
Lymph Nodes
;
Lymphadenitis
;
Male
;
Necrosis
;
Neutrophils
;
Tuberculosis
;
Tuberculosis, Lymph Node*
4.A case of transfusion-related acute lung injury induced by anti-human leukocyte antigen antibodies in acute leukemia.
Sun Mi JIN ; Moon Ju JANG ; Ji Young HUH ; Myoung Hee PARK ; Eun Young SONG ; Doyeun OH
Korean Journal of Hematology 2012;47(4):302-306
Transfusion-related acute lung injury (TRALI) is a noncardiogenic pulmonary edema that occurs during or within 6 hours after transfusion. Risk factors for TRALI, which is relatively common in critically ill patients, include recent surgery, hematologic malignancy, and sepsis. Here, we report a case of TRALI induced by anti-human leukocyte antigen (anti-HLA) class II antibodies (HLA-DR) occurring after transfusion of platelet concentrates in a patient with acute leukemia. Although most patients with TRALI show improvement within 48-96 hours, our patient's condition rapidly worsened, and he did not respond to supportive treatment. TRALI is a relatively common and serious adverse transfusion reaction that requires prompt diagnosis and management.
Acute Lung Injury
;
Antibodies
;
Blood Group Incompatibility
;
Blood Platelets
;
Critical Illness
;
Hematologic Neoplasms
;
Humans
;
Leukemia
;
Leukocytes
;
Pulmonary Edema
;
Risk Factors
;
Sepsis
5.Cloning and DNA sequencing of flagellin gene of helicobacter pylori.
Kwang Ho RHEE ; Won Kon LEE ; Myung Je CHO ; Young Mi DOH ; Seung Chul BAIK ; Kyung Hee KANG ; Pill Sung PARK ; Sang Yong LEE
Journal of the Korean Society for Microbiology 1993;28(1):23-35
No abstract available.
Clone Cells*
;
Cloning, Organism*
;
DNA*
;
Flagellin*
;
Helicobacter pylori*
;
Helicobacter*
;
Sequence Analysis, DNA*
6.Monoclonal antibodies and DNA sequence of 120 kd antigen ofhelicobacter pylori.
Kwang Ho RHEE ; Woo Kon LEE ; Seung Chul BAIK ; Young Mi DOH ; Myung Je CHO ; Hyu Jin CHOI ; Hang Hee RYOU
Journal of the Korean Society for Microbiology 1992;27(2):143-154
No abstract available.
Antibodies, Monoclonal*
;
Base Sequence*
;
DNA*
7.Development of a diagnostic method of helicobacter pylori infection: II. detection of helicobacter pylori using polymerase chain reaction.
Cheol Keun PARK ; Woo Kon LEE ; Young Mi DOH ; Hyu Jin CHOI ; Myung Je CHO ; Hee Shang YOUN ; Kwang Ho RHEE ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1992;27(1):45-58
No abstract available.
Helicobacter pylori*
;
Helicobacter*
;
Polymerase Chain Reaction*
8.Development of a diagnostic method of helicobacter pylori infection: II. detection of helicobacter pylori using polymerase chain reaction.
Cheol Keun PARK ; Woo Kon LEE ; Young Mi DOH ; Hyu Jin CHOI ; Myung Je CHO ; Hee Shang YOUN ; Kwang Ho RHEE ; Woo Hyun CHANG
Journal of the Korean Society for Microbiology 1992;27(1):45-58
No abstract available.
Helicobacter pylori*
;
Helicobacter*
;
Polymerase Chain Reaction*
9.A Study of Individual Variation of Responsiveness to Vagal Stimulation in Children (Nonspectral Analysis).
Mi Ryoung KIM ; Seong Hwan KIM ; Young Keun OH ; Byung Kiu PARK ; Moon Hong DOH ; Bong Kwan SEO ; Myung Kul YUM
Korean Circulation Journal 1992;22(1):96-104
BACKGROUND: It is well known that there is wide individual variation of responsiveness when parasympathetic nervous system is stimulated physiologically, pathologically, and therapeutically. But no appropriate explanation is present for that yet. So the purpose of this study is to verify individual diversity of vagal tone during resting state and after vagal stimulation and to find factor that may significantly contribute to it. METHOD: We serially measured variation of heart period(VHP : sec) which had been known to be an accurate index of cardiac parasympathetic tone during resting state(VHPb) and after vagal stimulation by ice water application to face(VHPv). And we analyzed correlation between VHPb, VHPv and ratio of VHPv to VHPb(ratio). RESULT: There was wide variation of vagal tone both in resting state (VHPb : 0.025-0.161) and after vagal stimulation (VHPv : 0.087-0.661). VHPb could be classified into 3 groups according to stem and leaf distribution(group A : VHPb could be classified into 3 groups according to stem and leaf distribution(group A : VHPb<0.07, B : 0.07-0.1, C : >0.1). The low VHPb group A showed dramatic increase in vagal tone after vagal stimulation (ratio 2.21-20.24 mean 7.7), on the other hand high VHPb group C did not (ratio 0.80-2.55 mean 1.39). There ws also a significant negative correlation between VHPb and ratio (r=0.69, p<0.001). CONCLUSION: These results suggest that there is wide individual diversity of responsiveness to vagal stimulation and magnitude of vagal tone in resting state is an important factor to determine the responsiveness to vagal stimulation.
Child*
;
Hand
;
Heart
;
Humans
;
Ice
;
Parasympathetic Nervous System
;
Water
10.The Onset of Coronary Artery Aneurysm and Changes of T Cell Subsets and the Effect of High Dose Gammaglobulin Therapy in Mucocutanenous Lymph Node Syndrome.
Seung Hwan KIM ; Mi Ryoung KIM ; Young Gyun OH ; Byung Kiu PARK ; Moon Hong DOH ; Bong Kwan SEO ; Myung Kul YUM
Korean Circulation Journal 1992;22(2):269-279
We studied the onset of coronary artery aneurysm formation in 42 patients of Mucocutaneous lymph node syndrome (MCLS), the serial changes of T cell subsets, and serum immunoglobulin levels including lgG, lgA, lgM, in 13 patients among them during acute (before high dose gamma-globulin therapy ) and subacute phase (after gamma-globulin therapy) to evaluate the role of the immunoregulatory abnormalities in coronary artery aneurysm formation. The following results are obtainded : 1) The coronary artery aneurysm was found in 16 patients (38%). They were detected in between 6th and 13th day of illness with the mean of 8.8+/-2.1 day. 2) The total T lymphocyte (T1), helper T lymphocyte (T4), and helper/supperessor ratio (T4/T8) decreased during acute phase. 3) Following gamma-globulin therapy it showed a increase in T4,T8, and all immunoglobulines. Above results showed that the most of aneurysms developed during acute phase and suppressed, rather than enhanced, T cell function significantly contributed to aneurysm formation, whereas high dose gamma-globulin retarded or reversed this suppression which would be one of mechanism of beneficial effort of gamma-globulin in MCLS.
Aneurysm*
;
Coronary Aneurysm
;
Coronary Vessels*
;
gamma-Globulins
;
Humans
;
Immunoglobulins
;
Lymph Nodes*
;
Lymphocytes
;
Mucocutaneous Lymph Node Syndrome
;
T-Lymphocyte Subsets*