PURPOSE AND BACKGROUND: Recently several clinical studies suggested that maternal treatment with magnesium sulfate had protective effects against cerebral palsy in premature infants. But previous studies with differing perinatal animal models resulted in inconclusive results with regard to magnesium neuroprotection. Our purpose was to study the neuroprotective effect of magnesium sulfate and optimal dosage on hypoxicischemic brain damage in the newborn rat. METHOD: Seven-day-old rats(n=68) underwent right carotid ligation, followed by 3 hours of hypoxia(8% oxygen in 92% nitrogen). Rats received magnesium sulfate immediately before and again after hypoxia(two doses, 150mg-600mg/kg/dose, n=39), or saline solution(n=29). Severity of injury was assessed 5 days later, by visual evaluation of ipsilateral hemisphere infarction and by measurement of bilateral hemispheric cross sectional areas. RESULTS: Magnesium sulfate pre-treatment reduced the incidence of liquefactive cerebral infarction and atrophy from 80.8% in controls to 22.2% with magnesium sulfate(450 mg/kg/dose, P<0.05). Quantitation of hemispheric areas confirmed these findings. Percent protection based on inter-hemisphere area differences by pre-treatment with magnesium sulfate 450mg/kg/dose ranged from 71.1%(hippocampus) to 90.8%(striatum). However higher dose of magnesium(600mg/kg/dose) did not attenuate hypoxic-ischemic brain injury in the newborn rat but increased mortality. CONCLUSION: Pretreatment of magnesium sulfate has neuroprotective effects against hypoxia-ischemia in the newborn rat and adequate dose of magnesium sufate is important to protect the brain. Magnesium pretreatment may be an effective strategy to decrease the severity of neonatal hypoxic-ischemic brain injury in the adequate dose.
Animals ; Atrophy ; Brain ; Brain Injuries ; Cerebral Infarction ; Cerebral Palsy ; Humans ; Incidence ; Infant, Newborn* ; Infant, Premature ; Infarction ; Ligation ; Magnesium Sulfate* ; Magnesium* ; Models, Animal ; Mortality ; Neuroprotective Agents* ; Oxygen ; Rats*

No abstract available.

No abstract available.

No abstract available.
Humans

In the present study, culture conditions to secrete proteinases from C. albicans, C. tropicalis and C. parapsilosis were examined. All three Candida species were found to secrete proteinases from acceleration phase to stationary phase, although the proteinase activities in culture filtrate were maximal during late exponential or early stationary phase. The proteinase activity in the culture filtrate of C. albicans cells grown at 30'C, was much higher than those grown at either 20 or 37'C. In culture of C. tropicalis and C. parapsilosis, the highest activity was found in culture filtrate grown at 37C. C. albicans secreted proteinases well in medium at initial pH 4.0-7.0. The optimal initial pH of medium for proteinase secretion was 7.0 for C. tropicalis and 5.0-6.0 for C. parapsilosis. All three Candida species secreted proteinases to greater amount in aerobic state. The most effective carbon source for proteinase secretion was xylose, glucose, maltose and sucrose for C. albicans, xylose for C. tropicalis and trehalose for C. parapsilosis. The effects of proteins, hydrolyzed proteins, ammonium sulfate as a sole nitrogen source on proteinase secretion were examined. Bovine serum albumin was the most effective nitrogen source of those tested and a little proteinase activity was detected in the culture filtrates when yeast cells were incubated in the medium containing ammonium sulfate. C. parapsilosis secreted proteinases to greater amount than the other Candida species in all nitrogen sources under study, indicating that C. parapsilosis proteinase would not be a inducible but a constitutive enzyme.
Acceleration ; Ammonium Sulfate ; Candida albicans* ; Candida* ; Carbon ; Glucose ; Hydrogen-Ion Concentration ; Maltose ; Nitrogen ; Peptide Hydrolases* ; Serum Albumin, Bovine ; Sucrose ; Trehalose ; Xylose ; Yeasts

No abstract available.
Dyspnea* ; Hypothyroidism*

Lymphangiectasia is a rsre occurrence, and may be due to an undgrlying disturbance of the lymph flow following surgery or irrediation. We present an unusual case of an extensive lymphangictasia of the vulva following radical abdominal hysterectomy and irradiation for cervical cancer.
Hysterectomy ; Uterine Cervical Neoplasms ; Vulva*

PURPOSE: This study is to analyze the research trends centering on the theses related to CAT released in nursing of Korea. METHOD: The researcher collected the academic degrees and theses published on the book of the academic society from 1980 to 2001, and examined 88 domestic dissertations on nursing science of the CAT. RESULT: 1) By department, the results were: the nursing thesis 88(43.3%), medical field 45(22.1%), psychology-counseling education 28(14%). 2) In the type of the CAT in nursing, the results were: acupressure 28(31.5%), Dan-Jeon Breathing 14(15.7%), aroma therapy 5(5.6%) in order. 3) The number of theses increased every year after 1997. 4) As for the type of study design, the results were: the experimental research had the most(47), and the survey research(30). 5) As for the subject, the results were: patients with disease(44), healthy adults(29), literature(9) and medical staff were only 6 theses. 6) In the theme of the theses, the results were: the effect of therapy(76), the experience of therapy(26), adaptation of nursing of therapy(11) and literature review were 7. CONCLUSION: More research needs to be encouraged in the unsatisfactory fields. Researches should be conducted to extend into the practical fields as Korean nursing mediation, project for the establishment of health policies should be conducted.
Acupressure ; Animals ; Aromatherapy ; Cats ; Education ; Health Policy ; Humans ; Korea* ; Medical Staff ; Negotiating ; Nursing* ; Respiration

Fifteen cases of genital warts were examined for the presence of human papillomavirus (HPV) using polymerise chain reaction (PCR). HPV6/11 DNA were found in all cases of genital warts. The PCR based methods described here provide a sensitive, accurate means of detecting genital HPVs.
Condylomata Acuminata ; DNA ; Humans* ; Papillomaviridae* ; Polymerase Chain Reaction*

No abstract available.
Pregnancy* ; Spermatozoa*