Due to the great deal of effort that has gone into the study of osteoclastic differentiation and activation over the last few decades, the mechanisms of these two events have been discovered gradually. Nitric oxide(NO-), which is produced from arginine by a nitric oxide synthase, opened up a new area of biological research. Recently, it has been reported that NO- is produced by osteoblasts stimulated by lipopolysaccharide and several other cytokines. In this study, the effect of sodium nitroprusside(SNP), a donor of nitric oxide(NO-), on osteoclast-like cell formation and on mature osteoclast function was examined. To determine the mechanism of the inhibitory effects of SNP decreased not only the basal 45Ca release but also thee bone resorption induced by PTH and l,25-dihydroxyvitamin D3 (I ,25[OH]2D3). The inhibitory effect of SNP on bone resorption induced by PTH appeared 2 days after treatment, whereas SNP effect on inhibiting bone resorption induced by l,25[OH]2D3 appeared at the third days. When chicken and rat osteoclasts were cultured on dentin slices, treatment of 300micronM SNP resulted in a significant decrease in dentin resorption by osteoclasts in terms of total resorption area and average individual area. We also examined the effect of SNP on formation of osteoclast-like cells that is TRAP-positive multinucleated cells from chicken and rat bone marrow cells in the presence or absence of 10(-8)M l,25[OH]2D3. The addition of 300 micronM SNP inhibited the formation of TRAP-positive multinucleated cells. The present data suggest that SNP, possibly as a NO- donor, inhibits the osteoclastic differentiation and osteoclastic activity.
Animals ; Arginine ; Bone Marrow Cells ; Bone Resorption ; Chickens ; Cytokines ; Dentin ; Humans ; Nitric Oxide Synthase ; Nitroprusside* ; Osteoblasts ; Osteoclasts* ; Rats ; Sodium* ; Tissue Donors

No abstract available.
Female ; Pregnancy ; Pregnancy, Ectopic*

To evaluate the reproducibility of the Ambulatory Electrocardiography (AECG), we examined the consistency rates of premature beats between the baseline AECG's and the repeat AECG's in 23 patients who underwent AECG's times within 1 year. 12 patients were male and 11 patients were female. Their mean age was 48 years. (Range ; 20 years-75 years) The time interval between both AECG's was 1 month 20 days. (Range ; 1 day-9months) They did not take any antiarrhythmic druge during the observation period except 4 patients who were taking calcium channel blockers or beta receptor blockers for hypertensive heart disease or ischemic heart disease. The consistency rate of both frequency and complexity of ventricular premature beats was 52%. The consisency rate of both frequency and complexity of supraventicular premature beats was 35%. These consistency rates seemed to vary as the time interval between both AECG's differed. In the case of ventricular premature beats, the consistency rates of 1 day, 8 days and 5 months as the time interval were 100%, 38% and 14% respectively. In the case of supraventricular premature beats, the consistency rates were 50%, 25% and 29%. We concluded that the reproducility of the AECG was low and this reproducibility became lower as the time interval between both AECG's became longer and that hese facts had to be considered when the effect of the antiarrhythmic drugs was evaluated.
Anti-Arrhythmia Agents ; Calcium Channel Blockers ; Cardiac Complexes, Premature ; Electrocardiography, Ambulatory* ; Female ; Heart Diseases ; Humans ; Male ; Myocardial Ischemia

Many investigators have shown that computer analysis of exercise induced ST segment changes in feasible and valid. This technique should make it possible to achieve uniformity of interpretation. Though myocardial ischemic response, arrhythmia, R wave amplitude and heart rate changes are documented by CASE exactly, blood pressure change, subjective and objective signs are assessed clinicall only. Therefore, both CASE and clinical analysis are demanded for the precise interpretation of GXT. Two hundred and ten patients, aged 22-68 y/o, were evaluated for comparative analysis between CASE and clinical assessment. The following results were obtained; 1) The sensitiivity of CASE to clinical analysis is 70.2%. 2) Among the 40 cases with ST segment-positive response, 24 cases(60%) had a less than 2mm ST depression and 16 cases(40%) had a more than 2mm ST depression. 3) Among the 40 cases with ST segment-positive response, 30 cases(75%) had a increased in R wave amplitude of 2.8mm in average, while among the 153 cases with GXT-negative response, 61 cases(39.9%) had R wave increase of 1.5mm in average. 4) Increment of systolic blood pressure per MET was 5.6 mmHg. There was no significant difference by age.
Arrhythmias, Cardiac ; Blood Pressure ; Depression ; Heart Rate ; Humans ; Research Personnel

The utility of computed axial tomography(CAT) in the diagnosis of spinal disorders has been the subject of considerable interest since the introduction of CAT scanning. Computed tomographic guided needle biopsy of 12 consecutive patients with a variety of undermined spinal disorders resulted in a 100% yield of positive tissue diagnoses, without signlficant complications. The principal advantage of cat guidance is the continuous direct observation of the needle tip position in relation to the target volume. We think that the application of computed tomographic guided needle biopsy in the lesions of the spine is of great value, because it is the single most accurate method of obtaining diagnosis other than by open surgical biopsy.
Animals ; Biopsy ; Biopsy, Needle ; Cats ; Diagnosis ; Humans ; Methods ; Needles ; Spine

A clinical study of the Tibial Shaft fracture was made on 128 patients, who had been treated at the Department of Orthopedic Surgery, College of Medicine Kyung Hee Universlty from the October, 1971 to May, 1979. The results were as follows: 1. The rate of fracture union was accelerated under the age of 20 years but it was slow in aged group. 2. The poor prognosis of fracture union was shown in junction between middle and distal 1/3 than others. 3. The better prognosis of the fracture type was shown in oblique and spiral fracture than in the comminuted and segmental. 4. There was prolonged rate of union in case of associated fibula fracture. 5. The frequency of delayed and nonunion were more prevalent in open fracture. 6. Among the open comminuted tibial shaft fracture with skin and soft tissue loss, the good results was obtained by the Hoffmanns external skeletal fixation method.
Classification ; Clinical Study ; Fibula ; Fracture Fixation ; Fractures, Open ; Humans ; Methods ; Orthopedics ; Prognosis ; Skin

It has been emphasized that treatment of hemoral neck fracture is early anatomical reduction and rigid internal fixation to reduce severe complications such as avascular necrosis or non-union.Operative treatment were developed recently with development of radiological technique. This study reviewed twenty-nine patients of femoral neck fracture treated by internal fixation between December 1973 and December 1979. There are eleven casesof Garden's stage I or II fracture with multiple Knowles pinning and eighteen cases (All Gardens stage) treated with compression hip screw, and followed for a minimum of nine months with an average eighteen months. The results were as follows; 1. Eleven cases with minimal displaced fracture (Garden's stage I or II) treated by multiple Knowles pinning were revealed good results. 2. In total eighteen cases treated by compression hip screw, nine cases (Garden's stage I or II) were revealed good results and other nine cases (Garden's stage I or II) were revealed fair or poor results. 3. Eleven cases of mlutiple Knowles pinning and nine case of compressian hip screw used for undisplaced fracture (Garden's stage I or II) were revealed good results without regard to internal fixation devices. 4. Avascular necrosis were four cases in nine cases(44%) of displaced fracture. 5. Non-union were not found in this aeries.
Agriculture ; Femoral Neck Fractures ; Femur Neck ; Hip ; Humans ; Internal Fixators ; Neck ; Necrosis

Purpose: To evaluate the clinical outcomes of a non-diffractive extended-depth-of-focus (EDoF) intraocular lens in eyes by epiretinal membrane (ERM) stage. Methods: Clinical records of 289 eyes with AcrySof® IQ Vivity TM (Alcon Laboratories) implantation were reviewed. Eyes were grouped according to ERM stage as no ERM (n = 228), stage 1 (n = 29), stage 2 (n = 20), and stage 3 (n = 12) ERM. Corrected and uncorrected distant visual acuity (CDVA and UDVA, respectively), uncorrected intermediate and near visual acuity (UIVA and UNVA), contrast sensitivity detected by the area under the log contrast sensitivity function (AULCSF), Strehl ratio, area ratio, and intraocular aberration were compared. Results: There was no difference in UDVA, UIVA, UNVA, or CDVA between groups (p = 0.639, p = 0.871, p = 0.963, and p = 0.051, respectively). There was no significant difference in Strehl ratio (p = 0.208), area ratio at 4 mm (p = 0.434), area ratio at 5 mm (p = 0.511), or ocular aberrations (p = 0.311 for total aberration). The AULCSF showed significant differences during both day (1.48 ± 0.21, 1.41 ± 0.18, 1.33 ± 0.17, and 1.31 ± 0.19 for no ERM, stage 1, stage 2, and stage 3 ERM, respectively; p = 0.036) and night (1.21 ± 0.21, 1.22 ± 0.12, 1.02 ± 0.20, and 1.08 ± 0.10 for no ERM, stage 1, stage 2, and stage 3 ERM; p = 0.027). Conclusions Eyes with stage 3 ERM without outer retinal involvement may benefit from EDoF technology when there is no metamorphopsia and when accompanied by timely membrane removal.

BACKGROUND: Limited ischemic tolerance of the lung has remained one of the factors that limits the expansion of pulmonary transplantation as a treatment for end-stage pulmonary disease. Numerous studies on safe long term preservation for lung transplantation has been performed for the purpose of developing ideal preservation solution with extracellular type or intracellular type solutions. In this study, we examined the efficacy of LPDG solution in lung preservation longer than 20 hours by comparison with modified Euro-Collins solution. METHODS: Thirty-four adult mongrel dogs were divided into two groups. Donor lungs were flushed with LPDG solution(n=9) or modified Euro-Collins(MEC) solution(n=8) and stored for 24 hours at 10degrees C. All donor lungs were perfused through the pulmonary arteries with solutions containing prostaglandin E1 and verapamil. Left canine lung allotransplantations wereperformed. Assessment(hemodynamic indices and arterial blood gas analysis) of left implanted lung was made by occluding the right pulmonary artery for ten minutes using pulmonary artery Cuff. Assessment was repeated at the interval of 30 minutes, one hour, and two hours later after reperfusion and then chest X-ray, computed tomogram and lung perfusion scan were obtained. In survival dogs follow-up studies were done with assessment with chest X-ray, computed tomogram of the chest and lung perfusion scan on 7th day postoperatively. After preservation above 20 hours, pathological examinations for ultrastructural findings on right lung were performed in each group. RESULTS: With respect to arterial oxygen tension, LPDG group was superior to MEC but there was no statistical significance for 2 hours after reperfusion. Mean pulmonary artery pressure was less increased(p<0.05) and cardiac output higher(p<0.05) than MEC group until 2 hours after reperfusion. After 2 hours of reperfusion, both groups showed transplanted lung function deteriorated gradually. Perfusion scan of the transplanted lung in LPDG group showed better perfusion rate in immediate post-reperfusion, 3 days and 7 days later respectively but there was no statistical significance and corelation with PaO2 and computed tomographic views. In scanning electron microscopy of pulmonary artery after preservation, LPDG group relatively shows less irregular protrusion of the inner surface of endothelial cell of poulmonary artery than MEC group. CONCLUSIONS: We concluded that LPDG solution can offer safe lung preservation above 20 hours with adequate immunosuppressive therapy and prevention of the infection.
Adult ; Alprostadil ; Animals ; Arteries ; Cardiac Output ; Dogs ; Endothelial Cells ; Follow-Up Studies ; Humans ; Lung Diseases ; Lung Transplantation ; Lung* ; Microscopy, Electron, Scanning ; Organ Preservation ; Oxygen ; Perfusion ; Pulmonary Artery ; Reperfusion ; Thorax ; Tissue Donors ; Verapamil

Insulin autoimmune syndrome (IAS) includes fasting or reactive hypoglycemia, hyperinsulinemia and the presence of insulin-binding antibodies in patients who have never been exposed to exogenous insulin. This report concems a 29-year-old male patient with Graves disease who had history of having taken methimazole for two months, without any consequence, 6 months previously. However, when methimazole was administered again for three weeks, the patient suffered hypoglycemia during the next fourth week. He denied history of diabetes mellitus (DM), of taking any oral hypoglycemic agent or of having received insulin injection. Laboratory data showed total serum insulin level > 300 pu/mL, C-peptide reactivity (CPR) 8.0ng/mL and insulin antibody 89%. After stopping methimazole, he was treated with radioiodine (131I). There was no episode of hypoglycemic attack during 8 months of follow-up.
Adult ; Antibodies ; C-Peptide ; Diabetes Mellitus ; Fasting ; Follow-Up Studies ; Graves Disease* ; Humans ; Hyperinsulinism ; Hypoglycemia ; Insulin ; Male ; Methimazole