1.Expression of hMSH2, hMLH1 Protein in Sporadic Colorectal Cancer and Corresponding Normal Tissue.
Jae Young JUNG ; Dong Kook PARK ; Ji Hyun SHIN
Journal of the Korean Society of Coloproctology 1998;14(4):709-718
PURPOSE: DNA mismatch repair gene is responsible for hereditary nonpolyposis colorectal cancer. But it is not well known its role in sporadic colorectal cancer patients. We analysed normal hMSH2, hMLH1 protein expression in colorectal adenocarcinoma tissues and corresponding normal tissues to find out the role of mismatch repair gene in sporadic colorectal cancer by Western blotting. METHODS: Normal hMSH2 and hMLH1 protein expression was studied on 25 colorectal cancer and corresponding normal tissue by Western blot with hMSH2 and hMLH1 monoclonal antibody. Normal protein band was expressed on 100 kD in hMSH2 and 87 kD in hMLH1. SW480 and LoVo cell line was used as positive and negative control for hMSH2 and LoVo and SW480 as positive and negative for hMLH1. And we analysed the relation between the hMSH2, hMLH1 protein expression and clinicopathological parameters. RESULTS: It was 2 cases (8%) that both hMSH2 and hMLH1 protein expression was not observed. Three cases (12%) were negative for hMSH2 and 2 cases (8%) for hMLH1. One or both hMSH2, hMLH1 protein expression was not observed in 7 cases (28%) in total. There was no correlation for proximal occurrence (25% vs 35%), young age (37.5% vs 23.5%) and lymph node metastasis (50% vs 47%). But poorly and mucinous differentiation was regarded as having relation with negative expression of hMSH2 and hMLH1 (50% vs 17.6%) but not significant statistically. CONCLUSION: Sporadic colorectal cancer with negative expression of normal hMSH2 and hMLH1 protein showed no relation to younger age, proximal site preference and lymph node metastasis. But it was suggested that mismatch repair gene protein was involved in cancer cell differentiation in sporadic colorectal cancer.
Adenocarcinoma
;
Blotting, Western
;
Cell Differentiation
;
Cell Line
;
Colorectal Neoplasms*
;
Colorectal Neoplasms, Hereditary Nonpolyposis
;
DNA Mismatch Repair
;
Humans
;
Lymph Nodes
;
Mucins
;
Neoplasm Metastasis
2.A survey on the actual situations and attitude of medical servicewith usual medical provider.
Seon Kook KWEON ; Young Lok SHIN ; Hyun Suk SHIN ; Young Chan LA ; Ki Hyung KIM
Journal of the Korean Academy of Family Medicine 1992;13(7):627-635
No abstract available.
3.Clinical review of 874 patients with gastric cancer.
Young Jin KIM ; Suk Jin HONG ; Shin Kon KIM ; Young Kook CHO
Journal of the Korean Surgical Society 1993;45(6):937-945
No abstract available.
Humans
;
Stomach Neoplasms*
4.Pulmonary paragonimiasis: CT findings.
Shin Ho KOOK ; Sang Gyeong SUH ; Sun Young NA ; Hae Su KWON ; Won Ja OH
Journal of the Korean Radiological Society 1992;28(5):711-714
Though the incidence of paragonimiasis has been remarkably decreased since 1970, it is still not a rare disease in Korea. Major problems in the diagnosis of pulmonary paragonimiasis on chest radiography are its differentiation from pulmonary tuberculosis and lung cancer. Chest radiographic findings have been described in detail, but little have been reported on CT findings. We reviewed CT findings of 10 patients with pulmonary paragonimiasis. The characteristic CT findings were similar to those on chest radiography, such as air-space consolidation (70%), nodular mass (50%), pleural effusion (40%), cystic lesion (30%), small low density within the mass (30%), linear density (20%), pneumothorax(20%), and burrow track (20%). CT depicted the cystic lesions and the burrow tracks more clearly and showed the small worm-retaining cysts within the mass that were not detectable on chest radiography. In conclusion, all of these CT findings are useful in the diagnosis of pulmonary paragonimiasis especially when differentiation from tuberculosis or lung canceris difficult on chest radiography.
Diagnosis
;
Humans
;
Incidence
;
Korea
;
Lung
;
Lung Neoplasms
;
Paragonimiasis*
;
Pleural Effusion
;
Radiography
;
Radiography, Thoracic
;
Rare Diseases
;
Thorax
;
Tuberculosis
;
Tuberculosis, Pulmonary
5.Cytosolic Glutathione S-Transferase Change after Deoxycholate Exposure in Colon Cancer Cell Lines.
Dong Kook PARK ; Ji Hyun SHIN ; Seok Gun PARK ; Sun Young CHEUNG
Journal of the Korean Society of Coloproctology 1998;14(4):701-708
PURPOSE: Bile acids (especially deoxycholate) was known to be toxic and mutagenic on colon epithelium. They proposed at least four mechanisms for the bile acid toxicity. It is the one of these mechanisms that bile acid inhibits the xenobiotic metabolizing enzyme activity (esp glutathione S-transferase, GST). So we measured the cytosolic GST level of colon carcinoma cell lines after deoxycholate exposure whether or not the deoxycholate lowered the cytosolic GST activity. METHODS: Three colon cancer cell lines (LoVo, SW480, HT29) were used for this study. We calculated the cellular toxicity by MTS method. And cytosolic GST activity was measured according to the method as Habig described. For total GST activity, 2.5 mM 1-chloro-2,4-dinitrobenzene was used for substrate, and measured as absorbance in 340 nm. RESULTS: Basal cytosolic GST level for LoVo, SW480, HT29 cell line was 514.59+/-27.01, 291.63+/-38.44 and 344.58+/-47.92 nmol/min/mg cytosol protein. GST level did not changed significantly after 5 days culture without DCA. But GST level was decreased significantly to 128.63+/-21.35, 134.33+/-41.76 and 163.10+/-22.73 nmol/min/mg cytosol protein each cell line after 5 days deoxycholate exposure (p<0.005). CONCLUSION: Cytosolic GST level was lowered significantly after deoxycholate exposure for 5 days. One of the mechanisms of bile acid toxicity for colon cancer cell is proposed to inhibit cytosolic GST activity.
Bile
;
Bile Acids and Salts
;
Cell Line*
;
Colon*
;
Colonic Neoplasms*
;
Cytosol*
;
Deoxycholic Acid*
;
Dinitrochlorobenzene
;
Epithelium
;
Glutathione Transferase*
;
Glutathione*
;
HT29 Cells
;
Humans
6.Disseminated Fusarial Infections in Two Children with Acute Leukemia.
Ji Young PARK ; Hoon KOOK ; Jong Hee SHIN ; Geun Mo KIM ; Sung Ho CHO ; Tai Ju HWANG
Korean Journal of Pediatric Hematology-Oncology 1997;4(2):363-369
Disseminated Fusarium infection has rarely been encountered in neutropenic cancer patients. The clinical features include fever, positive blood cultures, severe myalgias, disseminated ecthyma gangrenosum-like skin lesions, ocular symptoms and multi-organ-system involvement. The mortality rate reported so far exceeded 70% despite antifungal treatments. We report here the first two cases of disseminated fusarial infections in Korea. The first patient contracted pneumonia during severe neutropenic period following consolidation chemotherapy for acute myelogenous leukemia(AML). Fusarium oxyshrum was cultured from bronchoalveolar lavage fluid. The patient remains free of fungemia after successful antifungal treatment of high-dose amphotericin B and terbinafine. The second patient had relapsed AML and suffered from disseminated fusarial infection involving lungs with characteristic skin lesions. The patient succumbed to refractory leukemia and the fungal infection. A brief review of the literature ensues with the case report.
Amphotericin B
;
Bronchoalveolar Lavage Fluid
;
Child*
;
Consolidation Chemotherapy
;
Ecthyma
;
Fever
;
Fungemia
;
Fusariosis
;
Fusarium
;
Humans
;
Korea
;
Leukemia*
;
Lung
;
Mortality
;
Myalgia
;
Pneumonia
;
Skin
7.Laboratory and Clinical Study of Ceftizoxime Sodium (Epocelin(R)) in Infections of the Pediatric Age Group.
Keun Soo LEE ; Hahng LEE ; Jeh Hoon SHIN ; Kook Young MENG ; Dae Keun KIM ; Choon Won KIM
Journal of the Korean Pediatric Society 1984;27(7):685-701
No abstract available.
Ceftizoxime*
;
Humans
8.A Case of Global Aphasia Without Other Focal Neurologic Lateralizing Signs.
In Uk SONG ; Du Shin JEONG ; Kwang Ik YANG ; Tae Kyeong LEE ; Hyung Kook PARK ; Moo Young AHN
Journal of the Korean Geriatrics Society 2001;5(3):265-269
Global aphasia without other focal neurologic lateralizing signs (GWLS) is an uncommon stroke syndrome. The pathogenesis of GWLS has been thought as the result of multiple cardioembolism. However, a few cases of GWLS with a single lesion have been reported. We report an unusual case of GWLS with a single left superior temporal infarction. A 73-year-old right handed man was admitted with receptive and expressive language impairment without other focal neurologic lateralizing signs. The patient's global aphasia symptom has being 4 weeks after symptom onset. Brain CT showed only one lesion of left superior temporal lobe. Our case notes that the multiple lesions of the speech area are not only necessary to produce GWLS, and it is not necessarily imply a double lesion or an embolic causes.
Aged
;
Aphasia*
;
Brain
;
Hand
;
Humans
;
Infarction
;
Stroke
;
Temporal Lobe
9.A Case of Immature Intrapericardial Teratoma.
Hea Young SHIN ; Won Kju CHOE ; Hae Yong LEE ; Chong Kook LEE ; Keun Chang SONG ; Soon Hee JUNG
Journal of the Korean Pediatric Society 1994;37(1):129-134
This paper describes a case of intrapericardial teratoma in a 20-day-old female meonate, who suffered from cyanosis and respiratory difficulty. She was evaluated by echocardiography, chest CT and MRI, the suspected cyanosis and respiratory difficulty. She was evaluated by echocardiography, chest CT and MRI, the suspected intrapericardial mass was identified and surgically removed. The postopervative diagnosis was intrapericardial teratoma, grade II. This case is reported with brief review of some related literature.
Cyanosis
;
Diagnosis
;
Echocardiography
;
Female
;
Humans
;
Magnetic Resonance Imaging
;
Teratoma*
;
Tomography, X-Ray Computed
10.The 2nd Workshop on Publication of Korean Journal of Anesthesiology.
Seong Deok KIM ; Jong Hak KIM ; Yang Sik SHIN ; Yong Seok OH ; Byung Kook CHAE ; Young Kyo CHOI
Korean Journal of Anesthesiology 1996;30(3):243-244
No abstract available.
Anesthesiology*
;
Education*
;
Publications*