1.Rapid Detection of Mycobacteria usin Mycobacteria Growith Indicator tube(MGIT)and Ogawa Media.
Oh Gun KWON ; Hyun Mi CHO ; In Ho JANG ; Young UH ; Kap Jun YOON
Korean Journal of Clinical Microbiology 2000;3(2):116-120
BACKGROUND: As many as several weeks of incubation may be necessary for the recovery of mycobacteria when conventional culture media are used. Previous studies evaluating Mycobacteria Growth Indicator Tube (MGIT) as a rapid for the growth and detection of mycobacteria from clinical specimens have been reported. We compared MGIT with Ogawa media for the recovery of mycobacteria from clinical specimens. METHODS: Ninety nine clinical specimens received in the laboratory of Wonju Christian Hospital from June to September 199 were used for this study. The specimens from nonsterile body sites were digested, decontaminated, and concentrated, for culture and Ziehl-Neelsen stain, and specimen were inoculated onto MGIT tube and 3% Ogawa egg medium, and cultured for 8 weeks. RESULTS: Of the 38 specimens culture-positive for mycobacteria, 3 grew isolates in MGIT medium only, 8 grew isolates in Ogawa media only, and 27 grew isolates in both media. Mean (median, range) times to detection of mycobacteria were 13.7 (5.5, 2-48) days with MGIT and 19.6 (18, 13-37) days with Ogawa (P>0.05). The number recovered with MGIT plus Ogawa media was 24 (63.2%) within 14 days of receipt of specimen, and 31 (81.6%) within 21 days. The contamination rates were 31 % for MGIT and 1 % for Ogawa media. CONCLUSIONS: MGIT appears useful to quickly detect and identify mycobacteria from clinical specimens. However, because the number of culture-positive specimen in MGIT was not greater than those recovered with Ogawa media, MGIT should be used in combination with solid media to reduce turnaround times and increase the isolation rate.
Culture Media
;
Gangwon-do
;
Mycobacterium
;
Ovum
2.Rapid Detection of Mycobacteria usin Mycobacteria Growith Indicator tube(MGIT)and Ogawa Media.
Oh Gun KWON ; Hyun Mi CHO ; In Ho JANG ; Young UH ; Kap Jun YOON
Korean Journal of Clinical Microbiology 2000;3(2):116-120
BACKGROUND: As many as several weeks of incubation may be necessary for the recovery of mycobacteria when conventional culture media are used. Previous studies evaluating Mycobacteria Growth Indicator Tube (MGIT) as a rapid for the growth and detection of mycobacteria from clinical specimens have been reported. We compared MGIT with Ogawa media for the recovery of mycobacteria from clinical specimens. METHODS: Ninety nine clinical specimens received in the laboratory of Wonju Christian Hospital from June to September 199 were used for this study. The specimens from nonsterile body sites were digested, decontaminated, and concentrated, for culture and Ziehl-Neelsen stain, and specimen were inoculated onto MGIT tube and 3% Ogawa egg medium, and cultured for 8 weeks. RESULTS: Of the 38 specimens culture-positive for mycobacteria, 3 grew isolates in MGIT medium only, 8 grew isolates in Ogawa media only, and 27 grew isolates in both media. Mean (median, range) times to detection of mycobacteria were 13.7 (5.5, 2-48) days with MGIT and 19.6 (18, 13-37) days with Ogawa (P>0.05). The number recovered with MGIT plus Ogawa media was 24 (63.2%) within 14 days of receipt of specimen, and 31 (81.6%) within 21 days. The contamination rates were 31 % for MGIT and 1 % for Ogawa media. CONCLUSIONS: MGIT appears useful to quickly detect and identify mycobacteria from clinical specimens. However, because the number of culture-positive specimen in MGIT was not greater than those recovered with Ogawa media, MGIT should be used in combination with solid media to reduce turnaround times and increase the isolation rate.
Culture Media
;
Gangwon-do
;
Mycobacterium
;
Ovum
3.Endobronchial Brachytherapy for MalignantAirway Obstruction: Low Dose Rate Versus High Dose Rate.
Journal of the Korean Society for Therapeutic Radiology 1996;14(2):123-128
PURPOSE: This is a retrospective study to compare the palliation rates, survival rates and complications of low dose rate and high dose rate endobronchial brachytherapy in the management of malignant airway obstruction. METHODS AND MATERIALS: Forty three consecutive patients with malignant airway compromise from primary or metastatic lung tumors were treated with low dose rate(LDR) endobronchial Iridium-192 insertion (21 patients) between October 1988 and June 1992, and high dose rate(HDR) endobronchial brachytherapy(22 patients) between August 1992 and April 1994 with palliative aim. Flexible fiberoptic bronchoscopy under fluoroscopic control was utilized in all 91 provedures. Twenty seven LDR provedures delivereda dose of 5-7.5 Gy to a 1.0 cm radius respectively. RESULTS: Subjective and objective responses to treatments were evaluated on follow-up examinations by clinical examination, chest x-rays and CT scan of the chest had been demonstrated on 8 LDR patients and 10 HDR patients. CONCLUSION: The technique of LDR and HDR endobronchial brachytherapy is simple and well tolerated procedure with minimal morbidity. It provides excellent palliation by keeping airway patent in these short life-spanned patients.
Airway Obstruction
;
Brachytherapy*
;
Bronchoscopy
;
Follow-Up Studies
;
Humans
;
Lung
;
Radius
;
Retrospective Studies
;
Survival Rate
;
Thorax
;
Tomography, X-Ray Computed
4.Photon Energy Dependence of the Sensitivity of LiF TLDs Loaded with Thin Material.
Byongim J MIN ; Sookil KIM ; John J K LOH ; Young Kap CHO
The Journal of the Korean Society for Therapeutic Radiology and Oncology 1999;17(3):256-260
PURPOSE: An investigation has been carried out on the factors which affect the response reading of thermoluminescent dosimeters (TLD-100) loaded with thin material in high energy photon. The aim of the study was to assess the energy response of TLD-100 to the therapeutic ranges of photon beam. MATERIALS AND METHODS: In this technique, TLD-100 (abbreviated as TLD) chips and three different thin material (Tin, Gold, and Tissue equivalent plastic plate) which mounted on the TLD chip were used in the clinical photon beam. The thickness of each metal plates was 0.1 mm and TE plastic plate was 1 mm thick. These compared with the photon energy dependence of the sensitivities of TLD (normal chip), TLD loaded with Tin or Gold plate, for the photon energy range 6 MV to 15 MV, which was of interest in radiotherapy. RESULTS: The enhancement of surface dose in the TLD with metal plate was clearly detected. The TLD chips with a Gold plate was found to larger response by a factor of 1.83 in 10 MV photon beam with respect to normal chip. The sensitivity of TLD loaded with Tin was less than that for normal TLD and TLD loaded with Gold. The relative sensitivity of TLD loaded with metal has little energy dependence. CONCLUSION: The good stability and linearity with respect to monitor units of TLD loaded with metal were demonstrated by relative measurements in high energy photon (6~15 MV) beams. The TLD laminated with metals embedded system in solid water phantom is a suitable detector for relative dose measurements in a small beam size and surface dose.
Metals
;
Plastics
;
Radiotherapy
;
Tin
;
Water
5.High Versus Low Dose-Rate Intracavitary Irradiation for Adenocarcinoma of the Uterine Cervix.
Woo Chul KIM ; Gwi Eon KIM ; Eun Ji CHUNG ; Chang Ok SUH ; Soon Won HONG ; Young Kap CHO ; Juhn LOH
The Journal of the Korean Society for Therapeutic Radiology and Oncology 2000;18(1):32-39
PURPOSE: The incidence of adenocarcinoma of the uterine cervix is low. Traditionally, Low Dose R ate (LDR) brachytherapy has been used as a standard modality in the treatment for patients with carcinoma of the uterine cervix. The PURPOSE of this report is to evaluate the effects of the High dose rate (HL)R) brachytherapy in the patients with adenocarcinoma of the uterine cervix compared with the LDR. MATERIALS AND METHODS: From January 1971 to December 1992, 106 patients of adenocarcinoma of uterine cervix were treated with radiation therapy in the Department of Radiation Oncology, Yonsei University with curative intent. LDR brachytherapy was carried out on 35 patients and 7 1 patients w -re treated with HDR brachytherapy. In LDR Group, 8 patients were in stage I, 18 in stage II and 9 in st;ge III. External radiation therapy was delivered with 10 MV X-ray, daily 2 Gy fractionation, total dose 40-46 Gy (median 43 Gy), And LDR Radium intracavitary irradiation was performed with Henschke applicator, 22-59 Gy to point A (median 43 Gy). In HDR Group, there were 16 patients in stage I, 38 in stag <, II and 17 in stage III. The total dose of external radiation was 40-61 Gy(median 45 Gy), daily 1.8-2.0 3y. HDR Co-60 intracavitary irradiation was performed with RALS (Remote Afterloading System), 30-57 Gy (median 39 Gy) to point A, 3 times a week, 3 Gy per fraction. RESULTS: The 5-year overall survival rate in LDR Group was 72.9%, 6 1.9%, 45.0% in stage I, II, III, respectively and corresponding figures for HDR were 87.1%, 58.3%, 41.2%, respectively (p>0.05). There was no statistical difference in terms of the 5-year overall survival rate between HDR Group and LDR Group in a denocarcinoma of the uterine cervix. There was 1 1% of late complication rates in LDR Group and 27% in HDR Group. There were no prognostic factors compared HDR with LDR group. The incidence of the late complication rate in HDR Group stage II, III was higher than that in LDR Group ( 16.7% vs. 31.6% in stage II, 11. 1% vs. 35.3% in stage III, p>0.05). Although the incidence of radiation induced late complication rate was higher in HDR Group stage II and III patients than that in the LDR Group, statistical significance was not detected and within acceptable level. CONCLUSION: There was no difference in terms of 5-year survival rate and failure pattern in the patients with adenocarcinoma of the uterine cervix treated with HDR and LDR brachytherapy. Even late complication rates were higher in the HDR group it was an acceptable range. This retrospective study suggests that HDR brachytherapy seems to replace the LDR brachytherapy in the adenocarcinoma of the uterine cervix. However, further studies will be required to refine the dose rate effects.
Adenocarcinoma*
;
Brachytherapy
;
Cervix Uteri*
;
Female
;
Humans
;
Incidence
;
Radiation Oncology
;
Radium
;
Retrospective Studies
;
Survival Rate
6.Identificatiion,Antimicrobial Susceptibility an Epidemiology of Klebsiella species Isolated from Clinical Specimen.
Young UH ; Soon Deok PARK ; Jeong Seog SON ; Hyeun Gyeo LEE ; Hyun Mi CHO ; Kap Jun YOON ; Hyo Youl KIM
Korean Journal of Clinical Microbiology 2000;3(2):99-110
BACKGROUND: In recent years, the incidence of extended-spectrum beta-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. METHOD: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities were also analyzed. RESULT: Isolation rate of Klebsiella in order of decreasing frequency were K. pneumoniae (74:7%), K. oxytoca (12.1%), K. ozaenae(1.7%), K. planticola(1.0%), K. terngena(0.9%), and K, ornithinolytica (0.7%), respectively. K. rhinoscleromatis was not isolated. Compared with outpatients, increase of resistance rates of inpatients's Klebsiella isolates were 10% in ciprofloxacin, 15% in cefoperazone/sulbactam, and the others were ranged from 24% to 31%. Isolation rate of ESBL producing K. pneumoniae by double disk (DD) synergy test was 41%, and detection rates by antimicrobial agents were as follows: cefotaxime (95%), aztreonam (58%), and ceftriaxone (37%). Antimicrobial susceptibility rate with the exception of ampicillin and imipenem decreased from the range of 81%-96% on admission day to 29-62% after one week on admission. CONCLUSION: The isolation rates of K. planticola and K. terrigena were less than 1%. The proportion of ESBL producing K. pneumoniae was 41 %. And the vast majority of multidrug resistant Klebsiella including ESBL producing strains are acquired by hospitalization.
Ampicillin
;
Anti-Infective Agents
;
Aztreonam
;
beta-Lactamases
;
Cefotaxime
;
Ceftriaxone
;
Ciprofloxacin
;
Epidemiology*
;
Hospitalization
;
Humans
;
Imipenem
;
Incidence
;
Klebsiella pneumoniae
;
Klebsiella*
;
Outpatients
;
Pneumonia
;
Research Personnel
7.Identificatiion,Antimicrobial Susceptibility an Epidemiology of Klebsiella species Isolated from Clinical Specimen.
Young UH ; Soon Deok PARK ; Jeong Seog SON ; Hyeun Gyeo LEE ; Hyun Mi CHO ; Kap Jun YOON ; Hyo Youl KIM
Korean Journal of Clinical Microbiology 2000;3(2):99-110
BACKGROUND: In recent years, the incidence of extended-spectrum beta-lactamase (ESBL) producing Klebsiella has been steadily increased, and the newer species K. planticola and K terrigena, formerly regarded as nonpathogen, have been reported with astonishing frequency from human infectious processes by some investigators. The aim of this study is to elucidate the isolation rate and antimicrobial susceptibility of recent clinical Klebsiella isolates. METHOD: For the clinical Klebsiella isolates during the period of June 1999 to May 2000, isolation frequency of Klebsiella species by specimen, departments, age, and sex were analyzed. And antimicrobial susceptibilities were also analyzed. RESULT: Isolation rate of Klebsiella in order of decreasing frequency were K. pneumoniae (74:7%), K. oxytoca (12.1%), K. ozaenae(1.7%), K. planticola(1.0%), K. terngena(0.9%), and K, ornithinolytica (0.7%), respectively. K. rhinoscleromatis was not isolated. Compared with outpatients, increase of resistance rates of inpatients's Klebsiella isolates were 10% in ciprofloxacin, 15% in cefoperazone/sulbactam, and the others were ranged from 24% to 31%. Isolation rate of ESBL producing K. pneumoniae by double disk (DD) synergy test was 41%, and detection rates by antimicrobial agents were as follows: cefotaxime (95%), aztreonam (58%), and ceftriaxone (37%). Antimicrobial susceptibility rate with the exception of ampicillin and imipenem decreased from the range of 81%-96% on admission day to 29-62% after one week on admission. CONCLUSION: The isolation rates of K. planticola and K. terrigena were less than 1%. The proportion of ESBL producing K. pneumoniae was 41 %. And the vast majority of multidrug resistant Klebsiella including ESBL producing strains are acquired by hospitalization.
Ampicillin
;
Anti-Infective Agents
;
Aztreonam
;
beta-Lactamases
;
Cefotaxime
;
Ceftriaxone
;
Ciprofloxacin
;
Epidemiology*
;
Hospitalization
;
Humans
;
Imipenem
;
Incidence
;
Klebsiella pneumoniae
;
Klebsiella*
;
Outpatients
;
Pneumonia
;
Research Personnel
8.Solitary Cysticercus Granuloma of the Brainstem: Case Report.
Juno PARK ; Young Gu CHUNG ; Dong Jun LIM ; Tae Hyong CHO ; Hoon Kap LEE ; Ki Chan LEE ; Jung Keun SUH
Journal of Korean Neurosurgical Society 2000;29(10):1357-1359
No abstract available.
Brain Stem*
;
Cysticercus*
;
Granuloma*
9.Evaluation of Staging with MR Imaging in the Uterine Cervical Cancer.
Woo Cheol KIM ; Hae Jeong JEON ; Soon Gu CHO ; Young Kap CHO ; John K LOH
Journal of the Korean Cancer Association 1997;29(5):842-850
PURPOSE: Uterine cervical carcinoma is the most common cancer in Korean women. We evaluated the accuracy of magnetic resonance (MR) imaging in determining the stage and extent of disease in cervical carcinoma. MATERIALS AND METHODS: From January 1994 through December 1996, in all 35 patients, MR imaging was performed before any operative procedure. With a 1.5T superconducting magnet, TR (repetition time)/TE (echo time) of 483/9msec for T1-weighted images and 3750/98msec for T2-weighted images were used. All patients underwent radical hysterectomy or total abdominal hysterectomy and had detailed histologic evaluation. MR image were reviewed and compared with pathologic findings on the presence of tumor size, depth of stromal invasion and vagina extension. RESULTS: The accuracy of MRI in determination of stage was 74%. Its accuracy was 60% for the assessment of tumor size. Tumor size was underestimated in 6 patients (17%) and overestimated in 8 patients (23%). Tumor infiltration into the stroma was classified as no, partial, complete. The accuracy of MRI in cervical stromal invasion was 66%. CONCLUSION: MR is a relatively promising method for staging and evaluating extent of disease in carcinoma of the uterine cervix.
Cervix Uteri
;
Female
;
Humans
;
Hysterectomy
;
Magnetic Resonance Imaging*
;
Surgical Procedures, Operative
;
Uterine Cervical Neoplasms*
;
Uterine Neoplasms
;
Vagina
10.Comparison of the Flowcytometric HLA-B27 Determination Methods.
Soo Young YOON ; Chang Kyu LEE ; Yoon Jeong CHO ; Kyung Ran MA ; Young Kee KIM ; Kap No LEE
Korean Journal of Clinical Pathology 1998;18(2):245-249
BACKGROUND: The flowcytometric analysis of HLA-B27 gives more objective results and is performed more rapidly than traditional serologic methods. We have used a flowcytometric method using only anti-HLA-B27 monoclonal antibody, but it gave frequently borderline mean fluorescence intensity (MFI) results. The authors compared the method using anti-HLA-B27 antibody (HLA- ABC-m3, Serotec) with the Becton Dickinson (BD) method which uses different HLA-B27 antibody (GS145.2) with CD3 antibody. METHODS: The 59 patients that requested HLA-B27 testing were measured by two methods. In the former method, the mononuclear cells were stained with HLA-B27-FITC and the MFIs were determined in lymphocytes. In the BD method, the whole blood was directly stained with CD3-PE and HLA-B27-FITC. The MFIs were determined in the CD3+ cells, and compared with the MFI of the standard. For the cases showing discrepancy in the two methods or borderline values, the HLA-ABC typing was done. RESULTS: Of 21 showing discrepancy, 10 samples had undergone HLA typing. Among nine samples that were positive by the Serotec method but negative by the BD method, four samples were B7, one B40, one B54 and three B7 CREG negative. One that was negative by the Serotec method but positive by the BD method was confirmed as HLA-B27. CONCLUSIONS: The Serotec method showed significant overlap between the MFIs of HLA-B27 and non B27 samples that resulted in a relatively low efficiency compared with the BD method. The discrepant results of the two methods seem to be due to maily the specificity of the antibody used.
Fluorescence
;
Histocompatibility Testing
;
HLA-B27 Antigen*
;
Humans
;
Lymphocytes
;
Sensitivity and Specificity