No abstract available.
Respiratory System* ; Ventilators, Mechanical*

BACKGROUND: The generation of the invasiveness in transfromed cells represents an essential step of tumor progression. The primary cause of the scattering of the cells in invasive carcinoma is a loss of the integrity of the intercellular adherens junction often involving loss of a functional cell-cell adhesion molecule E-cadherin. Therefore, the perturbation of E-cadherin function causes diaggregation of tumor cells and may promote the invasion and metastases. OBJECTIVE: The reduction in E-cadherin activity seems to correlate with the infiltrative ability of tumor cells. The purpose of this study was to compare the E-cadherin expression among different cell lines which were normal to undifferentiated and to check the virtual relationaship between E-cadherin and invasiveness. METHODS: We used 5 cell lines, HaCaT, A431, C3, SiHa and HeLa cell. To check the expression patterns and amounts of E-cadherin in each cell line, immunofluorescence staining, Western blot anlysis and Northern blot analysis were done. An in vitro invasion assay using the collagen gel and MRC-5 fibroblast under the influence of HECD-1 antibody which block the E-cadherin function was done to measure the invasiveness of tumor cells. Collagenase activity in culture supernatants of each cell were analyzed by zymography. RESULTS: Immunofluorescence staining revealed a homogenously well preserved pattern in HaCat, A431, C3 cells. SiHa cells showed patch distribution but HeLa cells did not express the E-cadherin. Western blot analysis and Northern blot results largely corresponded with the immunofluorescence results. The in vitro invasion assay revealed invasion into the collagen matrix of the HeLa cells. When HECD-1 antibody was added to the medium, other cells showed partially disrupted stratification. The collagenolytic activity at 72 kDa sixe was detected in the HeLa cell line only. CONCLUSION: There is an inverse relationship between E-cadherin expression and tumor invasion. Therefore, through their regulation of cell adhesion and motility, cadherin plays a crucial role in the suppression of tumor invasion and metastasis.
Adherens Junctions ; Blotting, Northern ; Blotting, Western ; Cadherins* ; Cell Adhesion ; Cell Line* ; Collagen ; Collagenases ; Fibroblasts ; Fluorescent Antibody Technique ; HeLa Cells ; Humans ; In Vitro Techniques ; Neoplasm Metastasis

No abstract available.
Child* ; Cystitis* ; Eosinophils* ; Humans

No abstract available.
Continuous Positive Airway Pressure* ; Hypercapnia* ; Pneumonia* ; Pulmonary Edema*

Formaldehyde is contained in various everyday products such as shampoos, detergents, leathers and papers, and is thought to be one of the important couses of clothing related contact dermatitis(ACD). The results about the minimum sensitizing concentrations of formaldehyde were different, and the concentration of 30 pg per gram of textiles was the least one. We measured the quantity of formaldehyde in 16 different types of textiles which are widely used in Korea to investigate whether the textiles contain an enough amount of formaldehyde to induce ACD and washing can reduce the amount of it. The results were as follows : 1. The quantity of formaldehyde ranged from 3 to 1246 pg per gram of textiles. 2. Three types textiles contained more than 100pg of formaldehyde per gram of textiles and 6 between 50 pg and 100pg. 3. 1.00% cotton, ramie and rayon contained the highest content of formaldehyde per gram fabric. 4. After washing, the average quantity of formaldehyde decreased to 60% of that, of pre-wash measure.
Boehmeria ; Clothing ; Detergents ; Formaldehyde* ; Korea ; Textiles*

No abstract available.
Extracellular Matrix* ; Glucose* ; Mesangial Cells*

Foot drop from paralysis of the muscles of the anterior and lateral compartments of the leg is common in leprosy. Surgical correction of foot drop in leprosy appears to have been neglected until Brand pointed out that anterior transposition of the tibialis posterior tendon to the intermediate cuneiform bone gave encouraging results. Many methods of correcting the functional problems associated with a foot drop have been tried with the object of providing a foot that is stable and socially acceptable. Fifteen cases of foot drop admitted to the Hospital of Leprosy Mission in Taegu, Korea during past 3 years from 1965 to 1968 were reviewed.
Daegu ; Foot ; Korea ; Leg ; Leprosy ; Muscles ; Paralysis ; Tarsal Bones ; Tendons

Malgaigne fracture is relatively rare and has many difficult problems for treatment. The author reviewed 15 cases of Malgaigne fracture treated at the orthopedic department of Kyungpook National University Hospital during the period from January 1963 to July 1969, and analyzed as to the cause of injury by mechanical forces, type of fracture, average duration for treatment, complication and end results. The clinical results were obtained as follows: 1. The causes of injury were mostly traffic accident (66.6%), cool miner accident (20%) and falling and stumbling(13.4%). 2. Males are roughly 5 times more frequent than females and almostly 4th to 5th decade of life. 3. Vertical shear type is the most common type in this series and had not been experienced the type of total pelvic disruption. 4. The most common complication is bladder and urethral injury and experienced a case of multiple long bone fracture. 5. All cases were treated by skeletal traction except 2 cases which were treated by skin traction. 6 Time of traction is 8 to 16 weeks (average 12 weeks) and observation was done 10 of 15 cases for 2 to 25 months (average 15 months).
Accidental Falls ; Accidents, Traffic ; Female ; Fractures, Bone ; Gyeongsangbuk-do ; Humans ; Male ; Miners ; Orthopedics ; Skin ; Traction ; Urinary Bladder

No abstract available.
Lipomatosis, Multiple Symmetrical*

PURPOSE: The purpose of this study was to evaluate the effects of magnetization transfer contrast(MTC) andfat-suppression(FS) in variable spin-echo and gradient-echo sequences for articular cartilage imaging and todetermine the optimal pulse sequences. MATERIALS AND METHODS: Using variable 7-pulse sequences, the knees of 15pigs were imaged Axial images were obtained using proton density and T2-weighted spin-echo (PDWSE and T2WSE),turbo spin-echo (TSE), multiplanar gradient-echo (MPGR), and 3D steady-state gradient-echo (3DGRE) sequences, andthe same pulse sequences were then repeated using MTC. Also T1-weighted spin-echo(T1WSE) and 3D spoiledgradient-echo(3DSPGR) images of knees were also acquired, and the procedure was repeated using FS. For each knee,a total of 14 axial images were acquired, and using a 6-band scoring system, the visibility of and thevisibilities of the the articular cartilage was analyzed. The visual effect of MTC and FS was scored using a4-band scale. For each image, the signal intensities of articular cartilage, subchondral bone, muscles, and salinewere measured, and signal-to-noise ratios(SNR) and contrast-to-noise ratios(CNR) were also calculated. RESULTS: Visibility of the cartilage was best when 3DSPGR and T1WSE sequences were used. MTC imaging increased the negativecontrast between cartilage and saline, but FS imaging provided more positive contrast. CNR between cartilage andsaline was highest when using TSE with FS(-3 5 1 . 1 +/-15.3), though CNR between cartilage and bone then fell to-1 4 . 7 +/-10.8. In MTC imaging using MPGR showed the greatest increase of negative contrast between cartilage andsaline(CNR change=-74.7); the next highest was when 3DGRE was used(CNR change=-34.3). CNR between cartilage andbone was highest with MPGR(161.9 +/-17.7), but with MTC, the greatest CNR decrease(-81.8) was observed. Thegreatest CNR increase between cartilage and bone was noted in T1WSE with FS. In all scans, FS provided acartilage-only positive contrast image, though the absolute value of CNR was lower than that of MTC imaging. CONCLUSION: The most prominent effects of MTC and FS were seen in MPGR and T1WSE, respectively, though forcartilage, optimal high signal intensity and contrast can be achieved using 3DGRE with MTC, and 3DSPGR with FS.
Cartilage ; Cartilage, Articular* ; Knee ; Magnetic Resonance Imaging* ; Muscles ; Protons