No abstract available.
Breast Feeding* ; Breast Neoplasms* ; Breast* ; Case-Control Studies* ; Female ; Humans ; Korea*

Tubular apocrine adenoma is a well-circumscribed intradermal benign tumor with tubular structures showing apocrine differentiation. Most of those cases occur on the scalp in middle aged women. Histologically, tubular structure with apparent apocrine decapitation secretion, cystic dilatation of tubule, and connection with epidermis are the characteristic features of tubular apocrine adenoma. We presented a case of tubular apocrine adenoma that had recurred after incomplete excision. It showed apocrine decapitation secretion histologically and both eccrine and apocrine differentiation on immunohistochemical study.
Adenoma* ; Decapitation ; Dilatation ; Epidermis ; Female ; Humans ; Middle Aged ; Scalp

BACKGROUND: A number of reports have been published regarding the use of imiquimod for the treatment of melanoma in situ and metastatic melanoma. Essential steps in the process of melanoma invasion and metastasis include degradation of basement membranes and remodeling of the extracellular matrix by proteolytic enzymes, including matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs). OBJECTIVE: To evaluate the antiinvasive effect of imiquimod in human malignant melanoma cell lines, SK-MEL-2 and SK-MEL-24, in vitro, and to investigate imiquimod-induced changes in the expression of MMPs and TIMPs. METHODS: Invasiveness of melanoma cell lines following imiquimod treatment was evaluated by invasion assays. In order to investigate the mechanism of the anti-invasive effect of imiquimod, mRNA and protein levels of MMP-2, -9, membrane type 1 (MT1)-MMP, TIMP-1, and -2 were assessed by real-time reverse transcription-polymerase chain reaction, gelatin zymography, and western blotting. RESULTS: Imiquimod treatment decreased in vitro viability of melanoma cells in a concentration-dependent manner. Imiquimod also elicited a concentration-dependent suppression of invasion in both melanoma cell lines. A concentration-dependent decrease in MMP-2 and MT1-MMP protein levels and a concentration-dependent increase in TIMP-1 and -2 protein levels by imiquimod was observed in both melanoma cell lines. However, expression of MMP-9 protein was increased in SK-MEL-2 but decreased in SK-MEL-24 with increasing imiquimod concentrations. Imiquimod elicited alterations in MMPs and TIMPs mRNA levels that parallel the observed changes in protein levels. CONCLUSION: Imiquimod may elicit an anti-invasive effect on human melanoma cells by regulating MMPs and TIMPs.
Basement Membrane ; Blotting, Western ; Cell Line ; Extracellular Matrix ; Gelatin ; Humans ; Matrix Metalloproteinase 14 ; Matrix Metalloproteinases* ; Melanoma* ; Membranes ; Metalloproteases* ; Neoplasm Metastasis ; Peptide Hydrolases ; RNA, Messenger ; Tissue Inhibitor of Metalloproteinase-1

No abstract available.
Rubinstein-Taybi Syndrome*

This study was undertaken to document the effect of transforming growth factor-beta2 (TGF-beta2(TGF-beta2) and basic fibroblast growth factor (bFGF) on the proliferation of pig retinal pigment epithelial cells (RPE). Whereas bFGF increased the proliferation, TGF-beta2 showed the inhibitory effect on the proliferation The inhibitory effect of TGF-beta2 disappeared in RPE subcultured with 10ng/ml of bFGF. Both TGF-beta2- and bFGF-specific antisense oligonucleotides blocked the autocrine effect of the growth factors. PLC-71 -specific antisense oligonucleotide inhibited the effect of TGF-beta2 and bFGF. Genistein inhibited the effect of TGF-beta2 and bFGF in dose-dependent man, ner. The data suggest the involvement. of in PLC-/1 and tyrosine kinase in signalling.
Epithelial Cells* ; Fibroblast Growth Factor 2 ; Genistein ; Intercellular Signaling Peptides and Proteins ; Oligonucleotides, Antisense ; Protein-Tyrosine Kinases ; Retinaldehyde* ; Transforming Growth Factor beta2*

No abstract available.
Extremities* ; Sarcoma*

PURPOSE: We studied prevention effects of vertical transmission of Hepatitis B with follow-up, through the change of Anti-HBs titers during 9 months after injection of Hepatitis B immunoglobulin (HBIG) and Hepatitis B vaccine (HBV) at birth in newborn delivered from Hepatitis antigen carrier mothers. METHODS: This study was performed on newborn delivered from HBsAg carrier mothers at Hae Sung Hospital from Feb. 1995 to May 1996. These newborn were injected intramuscularly with HBV and HBIG after evaluation of HBsAg and Anti-HBs titer directly at birth. Regular Hepatitis vaccination was given at 1 month, 6 months as well as HBsAg and Anti-HBs titer were evaluated at 24 hours, 1 month, 3 months, 6 months, and 9 months. RESULTS: The incidence of Hepatitis B carrier mothers is 5.6%, and newborn babies born to HBsAg carrier mothers showed HBsAg in 10.6%. In most newborn, Anti-HBs titer maintained in 100-1,000mIU/ml after injection of HBIG and HBV at birth, and mean Anti-HBs titer decreased a little at 1 month, but therafter increased gradually by regular Hepatitis vaccination. HBsAg positive newborn maintained mean HBsAg titers below 2 (S/N) until 9 months old after injection of HBIG and HBV. CONCLUSIONS: A screening test for Hepatitis B must be performed on all pregnant women, and that infants of Hepatitis B carrier mothers must be immunized by HBIG and HBV directly at birth.
Female ; Follow-Up Studies ; Hepatitis ; Hepatitis B ; Hepatitis B Surface Antigens* ; Hepatitis B Vaccines ; Humans ; Immunoglobulins ; Incidence ; Infant ; Infant, Newborn* ; Mass Screening ; Mothers* ; Parturition ; Pregnant Women ; Vaccination

No abstract available.
Cyclosporine* ; Humans

Ota nevus is a dermal melanocytic harmatoma derived from neural crest melanoblast and involves skin innervated by the trigeminal nerve. Although most cases are clinically apparent at birth or around puberty, acquired lesions in adults have been rarely reported. We reported a 73 year-old Korean man with Ota nevus of the face and scalp that onset in his eighth decade and accompanied multiple solar comedo of the face.
Adolescent ; Adult ; Humans ; Neural Crest ; Nevus of Ota* ; Parturition ; Puberty ; Scalp ; Skin ; Trigeminal Nerve

Mixed tumor of the skin (chondroid syringoma) is a rare benign tumor composed of epithelial elements intermingled with myxoid or cartilagenous stroma which is not separated by basement membrane. It had been believed to originate from the eccrine gland but recently, it was described to be of apocrine gland origin in case of showing apparent apocrine secretion. We report on a 63-year-old man with a tumor on the right upper eyelid showing typical microscopic features of mixed tumor of the skin. Many apocrine decapitation secretions were seen in tubular structures and follicular differentiations were also seen, which represents the common origin of folliculo-sebaceous-apocrine unit.
Adenoma, Pleomorphic ; Apocrine Glands ; Basement Membrane ; Decapitation ; Eccrine Glands ; Eyelids ; Humans ; Middle Aged ; Skin