Helicobacter pylori infection has been shown to be associated with gastric carcinoma. However, despite the frequent detection of seropositivity for H. pylori and histologic detection in biopsy specimen, histologic detection rate of H. pylori in surgical specimens has been low. In this study, we investigated the prevalence of H. pylori infection in gastrectomy specimens bearing gastric adenocarcinoma and compared it with both endoscopic biopsy and serologic results. H. pylori infection was identified by Giemsa stain in the mucosa stripped from the tumor, body, and antrum in 61 gastrectomy specimens. We evaluated the effect of H. pylori infection on gastric mucosal cell proliferation by using monoclonal antibody for Ki-67. H. pylori detection rate using Giemsa stain was higher in gastrectomy specimens (67.3%) compared to that (48.1%) of biopsy specimens (p=0.006). The detection rate was higher in body than that of antrum or tumor site in the same patients (p=0.001). The H. pylori seropositivity was 60.5% and relatively nonspecific. The mean value of Ki-67 labeling index in the H. pylori-positive group was higher than that in the H. pylori-negative group (p<0.05). The increase in gastric epithelial cell proliferation was not influenced by the location of the tumor or the site of the specimen. The results suggest that the actual prevalence of H. pylori infection in patients with gastric carcinoma is considerably higher than that evaluated on endoscopic biopsy specimens. In addition, the increased cell proliferation in the H. pylori-positive group suggests some evidence that H. pylori may be involved in gastric carcinogenesis.
Adenocarcinoma* ; Azure Stains ; Biopsy ; Carcinogenesis ; Cell Proliferation ; Epithelial Cells ; Epithelium* ; Gastrectomy ; Helicobacter pylori* ; Helicobacter* ; Humans ; Mucous Membrane ; Prevalence

No abstract available.
Carcinoma, Papillary* ; Thyroid Gland*

No abstract available.
Multiple Endocrine Neoplasia*

No abstract available.
Graves Disease* ; Humans ; Thyroid Gland*

The treatment of pest-inflammatory obstructive glaucoma present difficult and anxious problems. In my case, before acute secondary glaucoma attack, in the region of pupil adhesions between the iris and capsule of the lens was about 3/4 of total pupillary zone and goneoscopically mild grade one peripheral anterior synechia was noted about 6 o'clock area of right eye. But intraocular pressure was normal limit. Ten years after binocular chronic anterior uveitis treatment, right eye suddenly developed .acute anterior uveitis signs and symptoms with 60 mmHg(IOTA). After secondary glaucoma with uveitis of right eye developed, uveitis treatment and diamox and oral glycerol was not controlled intracoular pressure: Intracular pressure was over 30 mmHg (IOTA) against medical treatment for two weeks. After medical treatment for two weeks, intracoular pressure was not controlled because of post-inflammatory obstructive glaucoma developed. During the acute inflammatory phase of right eye, where an iridectomy, a transfixation of iris and filtrating operation is thought to be hazardous because of intraocular surgery. I want to control post-inflammatory obstructive glaucoma without intraocular surgery such as iridectomy or filtrating operation. By the method of cyclodiathermy, the ball type electrode was applied to the inferion half sclera along an arc 6 mm to 8 mm from the limbus at about 15 place several millimeter apart. After 4 months of cyclodiathermy applied, intraocular pressure has been about 18 mHg(IOTA) for 3 months and naked visual acuity of right eye is 0.6.
Acetazolamide ; Electrodes ; Glaucoma* ; Glycerol ; Intraocular Pressure ; Iridectomy ; Iris ; Pupil ; Sclera ; Telescopes ; Uveitis ; Uveitis, Anterior ; Visual Acuity

Breast cancer is one of the leading causes of death attributable to cancer in women. In view of the limitations of conventional predictable factors of the breast cancer, additional second-generation parameters would be valuable in selecting the patients who would be most likely to be beneficial from adjuvant therapy and breast reconstruction. The author investigated the HER2/neu gene amplification and the number of chromosome 17 in 39 cases of paraffin embedded breast cancer tissues, 20 cases without lymph node metastasis and 19 cases with lymph node metastasis, using fluorescent in situ hybridization(FISH) and compared the results with HER2/neu and p 53 protein expression detected by immunohistochemical method. Eleven cases fibroadenoma were used as benign tumor control. Numerical aberrations of chromosome 17 were found in 17 out 39 breast cancer cases (44%)(monosomy in 10 cases, 26%; trisomy in 3 cases, 8%; tetrasomy in 3 cases, 8%; polysomy in 1 case ,3%), and the frequency of each type aberration was not significantly different between the negative and positive groups in lymph node metastasis. Monosomy of chromosome 17 was found in 2 out of 11(12%) fibroadenoma cases. HER2/neu gene amplification was found in 8 out of 39 cases (19%) and other 2 cases revealed HER2/neu gene amplification in lymph node metastatic tumor only, not in original tumor. Fourteen out of 19 cases of breast cancer with lymph metastasis showed HER2/neu protein expression both in original and metastatic tumors. All of the six cases showing HER2/neu gene amplification in original and/or metastatic tumor revealed HER2/neu protein expression. The frequency of HER2/neu gene amplification in the 39 breast cancer cases was not different between metastatic and non-metastatic groups(p= 0.284). However, HER2/neu protein expression was increased significantly in the metastatic group(p=0.028). None of the 11 fibroadenoma cases revealed HER2/neu gene amplification or HER2/ neu protein expression. Nine out of 19 cases of breast cancer with lymph node metastasis showed p 53 protein accumulation in original tumor(47%), but 3 of them revealed p 53 protein accumulation only in original tumor. The frequency of p 53 protein accumulation was not significantly different between metastatic and non-metastatic groups. None of the 11 fibroadenoma cases revealed p 53 protein accumulation. In conclusion, there are no differences between the lymph node metastatic group and non-metastatic groups in numerical aberrations of the chromosome 17 , amplification of the HER2/neu gene expression and accumulation of the p 53 protein in breast cancer. However, the HER2/neu protein expression was increased significantly in lymph node metastatic group, so it could be one of the predictors of the metastasis in breast cancer.
Breast Neoplasms* ; Breast* ; Cause of Death ; Chromosomes, Human, Pair 17* ; Female ; Fibroadenoma ; Gene Amplification* ; Gene Expression ; Humans ; Lymph Nodes ; Mammaplasty ; Monosomy ; Neoplasm Metastasis ; Paraffin ; Tetrasomy ; Trisomy

Trichost.asis Spinulosa consists of several fasciles of reta ned vellus hairs in the hyperkeratotic sebaceous follicles, resulting in a dark slightly raied horny follicular spines, resembling comedones on the trunk and face. The condition is relatively common, particulary among the age, but is usually overlooked on routine skin examination as the patient is rarely aware of it.. We report 6 cases of trichostasis spinulosa, which showed numerous asymptomatic tiny black comedo-like dots on their backs. We obtained the follicular material by tweezer, which consised of several vellus hairs surrounded by a keratinous plug. Histologic examination was done in 3 patients, one of whom showed a rarely reproted double follicle and double papilla. With scanning electron microscopy, we observed multiple vellu hairs embedded in a keratotic plug, emerging from a hair follicle opening.
Hair ; Hair Follicle ; Humans ; Microscopy, Electron, Scanning ; Skin ; Spine

It is well known that a successive corneal graft depends primarily on the corneal endothelial viability The standard eye-bank method for the preserving donor's corneal tissue for transplantation is moist storage at 4 degrees C. The purpose of this study is to determine the change in permeabilty of the rabbit corneal endothelium for the purpose of evaluating the possible change in the endothelia] viability after varying duration of storage time ranging from 6 hours to 7 days in a moist chamber at 4 degrees C. To fully describe the permeability, the author measured the two main coefficient, hydraulic conductivity (Lp) and reflection coefficient(sigma(NaCI)). In this study, the hydraulic conductivity of the rabbit corneal endothelium increased graduaIly during the first 3 days of storage time and then progressively decreased. Reflection coefficient was 0.4 on the first and second day and then decreased progressively. Both the hydraulic conductivity and the reflection coefficient showed a marked decrease on the 7th day of storage. Therefore, it is clear that the rabbit corneal endothelial cells almost lost their viability and function as physical barrier mechanism on the 7th day of storage. A comparison of the result of this permeability with those of other methods (N B T corneal endothelial staining and electron microscopic etc.) was discussed.
Endothelial Cells ; Endothelium, Corneal* ; Permeability* ; Transplants

It is well known that a successive corneal graft depends primarily on the corneal endothelial viability The standard eye-bank method for the preserving donor's corneal tissue for transplantation is moist storage at 4 degrees C. The purpose of this study is to determine the change in permeabilty of the rabbit corneal endothelium for the purpose of evaluating the possible change in the endothelia] viability after varying duration of storage time ranging from 6 hours to 7 days in a moist chamber at 4 degrees C. To fully describe the permeability, the author measured the two main coefficient, hydraulic conductivity (Lp) and reflection coefficient(sigma(NaCI)). In this study, the hydraulic conductivity of the rabbit corneal endothelium increased graduaIly during the first 3 days of storage time and then progressively decreased. Reflection coefficient was 0.4 on the first and second day and then decreased progressively. Both the hydraulic conductivity and the reflection coefficient showed a marked decrease on the 7th day of storage. Therefore, it is clear that the rabbit corneal endothelial cells almost lost their viability and function as physical barrier mechanism on the 7th day of storage. A comparison of the result of this permeability with those of other methods (N B T corneal endothelial staining and electron microscopic etc.) was discussed.
Endothelial Cells ; Endothelium, Corneal* ; Permeability* ; Transplants

Total hip replacement arthroplasty has become an established procedure in the management of painful arthritic hip in past two decades. However there is no many reports that concern follow-up study in total hip replacement arthropiasty. This is a follow-up study in 100 total hip replacement arthroplastics in 86 patients of 157 total hip replacement arthroplasties in 135 patients, performed at the Department of Orthopedic Surgery, Seoul National University Hospital, between November 1973 and December 1979. The longest fallow-up was 5 years and the shortest 6 months, the average being 2 years and 6 months. 1. There were 54 males (63%) and 32 females (37%). 2. The underlying diseases of 100 hips were avascular necrosis of 35 hips, septic hip residua of 31 hips, primary osteoarthritis of 9 hips, old hip fracture of 9 hips, Legg-Perthes disease of 7 hips, rheumatoid arthritis of 5 hips, congenital dislocation of 3 hips, and failed endoprosthesis of 1 hip. 3. The types of prosthesis used were Muller ones in 82 hips, T-28 in 17 hips, and Charniey and Harris type one case respectively. 4. In the half cases of a hundred total hip replacement arthroplasties, the anterolateral or posterolateral approach was used without trochanteric osteotomy. 5. Postoperative complications were as follows: 13 cases of wound problem including 3 early deep infections, 2 dislocations, 1 femoral nerve palsy, 3 peroneal nerve palsies, 1 acetabular perforation, 2 femoral shaft perforations, 14 trochanteric problems including 11 cases of wire breakage, 2 nonunions, and 3 cases of trochanteric bursitis 3 ectopic ossifications, 3 cases of radiological loosening including 2 femoral and 1 acetabular. 6. The range of motion were improved from 28 to 5 of flexion contracture, from 91 to 101 of further . flexion, and from 16 to 48 of abduction in the average. 7. The improved ranges of motion were varied with disease group rather than type of prosthesis and the length of neck of prosthesis. 8. The function of each hip was evaluated preoperatively and postoperatively by the method of Harris and d'Aubigne, and improved from 55.3 and to 88.2 by Harris score and from 11.2 to 15.8 d Aubigne respectively.
Acetabulum ; Arthritis, Rheumatoid ; Arthroplasty ; Arthroplasty, Replacement, Hip ; Bursitis ; Contracture ; Dislocations ; Female ; Femoral Nerve ; Femur ; Follow-Up Studies ; Hip ; Humans ; Legg-Calve-Perthes Disease ; Male ; Methods ; Neck ; Necrosis ; Orthopedics ; Osteoarthritis ; Osteotomy ; Paralysis ; Peroneal Nerve ; Postoperative Complications ; Prostheses and Implants ; Range of Motion, Articular ; Seoul ; Wounds and Injuries