1.The change of adaptability change in adhesive systems to dentin substrte according to storage time.
Young Gon CHO ; Il Hwan BAN ; Mi Kyung YU
Journal of Korean Academy of Conservative Dentistry 2005;30(3):204-214
This study compared the microtensile bond strength (microTBS) and microscopic change of two 2-step and two 1-step self-etching adhesives to dentin according to storage times in distilled water. Occlusal dentin was exposed in 48 human molars. They were divided to four groups by different adhesives: SE Bond group (Clearfil SE Bond), AdheSE group (AdheSE), Adper group (Adper Prompt L-Pop), and Xeno group (Xeno III). Each group was stored in 37degrees C distilled water for 1, 15, and 30 days. Resin-bonded specimens were sectioned into beams and subjected to microTBS testing with a crosshead speed of 1 mm/minute. For SEM observation, one specimen was selected and sectioned in each group after each stroage time. Resin-dentin interface was observed under FE-SEM. In all storage times, mean microTBS of SE group was significantly higher than those of other groups (p < 0.05). There was no significant difference between mean microTBS of SE group and AdheSE group among all storage times, but significant difference between 1- and 30-day storage in mean microTBS of Adper group and Xeno group (p < 0.05). For 1-and 15-day storage, all groups showed the close adaptation between resindentin interfaces. For 30-day storage, resin-dentin interfaces showed wide gap in Adper group and separate pattern in Xeno III group.
Adhesives*
;
Dentin*
;
Humans
;
Molar
;
Water
2.A Case of Intravenous Pyogenic Granuloma.
Duck Hwan WON ; Dae Hyun BAN ; Young Keun KIM ; Gwang Sung CHOI ; Joo Heung LEE ; Sang wahn KOO
Korean Journal of Dermatology 1999;37(11):1679-1681
An intravenous pyogenic granuloma is a rare, benign, intravascular tumor, which arises from the vein wall and protrudes into the lumen. This is characterized by a lobular proliferation of capillaries similar to the more common cutaneous pyogenic granulomas. We report a case of intravenous pyogenic granuloma which showed lobular capillary proliferation in the perivenous connective tissue.
Capillaries
;
Connective Tissue
;
Granuloma, Pyogenic*
;
Veins
3.Detection of Shiga Toxin-Producing Escherichia coli by in Stitu hybridization and sequence Analysis of Stx2.
Eui Chong KIM ; Dong Young LEE ; Hae Shim CHOI ; Se Ik JOO ; Jung Hee LEE ; Sang Hyun KIM ; Sung Hwan BAN
Korean Journal of Clinical Microbiology 2000;3(2):94-98
BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) was found in several serotypes of E coli including 0157 serotype. Sorbitol-MacConkey agar: may be useful for the detection of E. coli 0157, but is not helpful for the detection of sorbitol-fermenting STEC other than 0157. Moreover, some strains of E. colt 0157 can ferment sorbitol. In this study, in situ hybridization using DNA probe of shiga toxin was used for the isolation of STEC from the PCR-positive stool and -Sequenbe analysis of a part of shiga toxin gene was performed. METHODS: The stool was incubated in LB broth overnight and DNA was extracted from the culture fluid. Multiplex PCR was performed with primers for stxl and stx2 genes. Specimen showed PCR-positive was incubated on MacConkey agar and colonies were blotted with nitrocellulose membrane. Digoxigenin-labelled DNA probe for shiga toxin was made by PCR and the positive colonies were detected with anti-digoxigenin-alkaline phosphatase conjugate and nitroblue tetrazolium. Agglutination test with antisera was performed for the serotying and VTEC-RPLA kit was used for the toxin production. Sequence analysis of PCR products was performed with automatic sequence analyser. RESULTS: An stxl-negative, but stx2-positive PCR was observed in a three-year-old girl, who visited Kumi Hospital on July 19, 1999 complaining of vomiting and diarrhea. The positive colonies were isolated by in situ hybridization using stx2-specific DNA probe. The titers of stxl and stx2 by VTEC-RPLA test were negative and 1:64, respectively. Agglutination for the serotyping was not observed with all of the 0 antisera. 160-nucleotide sequence of stx2 of this isolate was identical with bacteriophage 933W (GenBank X07865), except for the change (T-C) of 957th nucleotide and amino acid sequence was identical each other. CONCLUSIONS: For the sensitive detection of STEC from the stool of patients with diarrhea, multiplex PCR is recommended with stxl- and stx2-specific primers. And in situ hybridization should be performed in PCR-positive specimen for the isolation of STEC. This method may be helpful for the detection of STEC as the causative microorganisms in food-borne outbreak.
Agar
;
Agglutination
;
Agglutination Tests
;
Amino Acid Sequence
;
Bacteriophages
;
Collodion
;
Diarrhea
;
DNA
;
Escherichia coli
;
Female
;
Gyeongsangbuk-do
;
Humans
;
Immune Sera
;
In Situ Hybridization
;
Membranes
;
Multiplex Polymerase Chain Reaction
;
Nitroblue Tetrazolium
;
Polymerase Chain Reaction
;
Sequence Analysis*
;
Serotyping
;
Shiga Toxin
;
Shiga-Toxigenic Escherichia coli*
;
Sorbitol
;
Vomiting
4.Detection of Shiga Toxin-Producing Escherichia coli by in Stitu hybridization and sequence Analysis of Stx2.
Eui Chong KIM ; Dong Young LEE ; Hae Shim CHOI ; Se Ik JOO ; Jung Hee LEE ; Sang Hyun KIM ; Sung Hwan BAN
Korean Journal of Clinical Microbiology 2000;3(2):94-98
BACKGROUND: Shiga toxin-producing Escherichia coli (STEC) was found in several serotypes of E coli including 0157 serotype. Sorbitol-MacConkey agar: may be useful for the detection of E. coli 0157, but is not helpful for the detection of sorbitol-fermenting STEC other than 0157. Moreover, some strains of E. colt 0157 can ferment sorbitol. In this study, in situ hybridization using DNA probe of shiga toxin was used for the isolation of STEC from the PCR-positive stool and -Sequenbe analysis of a part of shiga toxin gene was performed. METHODS: The stool was incubated in LB broth overnight and DNA was extracted from the culture fluid. Multiplex PCR was performed with primers for stxl and stx2 genes. Specimen showed PCR-positive was incubated on MacConkey agar and colonies were blotted with nitrocellulose membrane. Digoxigenin-labelled DNA probe for shiga toxin was made by PCR and the positive colonies were detected with anti-digoxigenin-alkaline phosphatase conjugate and nitroblue tetrazolium. Agglutination test with antisera was performed for the serotying and VTEC-RPLA kit was used for the toxin production. Sequence analysis of PCR products was performed with automatic sequence analyser. RESULTS: An stxl-negative, but stx2-positive PCR was observed in a three-year-old girl, who visited Kumi Hospital on July 19, 1999 complaining of vomiting and diarrhea. The positive colonies were isolated by in situ hybridization using stx2-specific DNA probe. The titers of stxl and stx2 by VTEC-RPLA test were negative and 1:64, respectively. Agglutination for the serotyping was not observed with all of the 0 antisera. 160-nucleotide sequence of stx2 of this isolate was identical with bacteriophage 933W (GenBank X07865), except for the change (T-C) of 957th nucleotide and amino acid sequence was identical each other. CONCLUSIONS: For the sensitive detection of STEC from the stool of patients with diarrhea, multiplex PCR is recommended with stxl- and stx2-specific primers. And in situ hybridization should be performed in PCR-positive specimen for the isolation of STEC. This method may be helpful for the detection of STEC as the causative microorganisms in food-borne outbreak.
Agar
;
Agglutination
;
Agglutination Tests
;
Amino Acid Sequence
;
Bacteriophages
;
Collodion
;
Diarrhea
;
DNA
;
Escherichia coli
;
Female
;
Gyeongsangbuk-do
;
Humans
;
Immune Sera
;
In Situ Hybridization
;
Membranes
;
Multiplex Polymerase Chain Reaction
;
Nitroblue Tetrazolium
;
Polymerase Chain Reaction
;
Sequence Analysis*
;
Serotyping
;
Shiga Toxin
;
Shiga-Toxigenic Escherichia coli*
;
Sorbitol
;
Vomiting
5.A Case of Relapsing Granulomatosis with Polyangiitis Treated Successfully with Rituximab.
Ji Won HAN ; Kwi Young KANG ; Tae Hyun BAN ; Ik Hyun JO ; Sung Hwan PARK
Journal of Rheumatic Diseases 2014;21(5):257-260
Granulomatosis with polyangiitis (GPA) is a rare antineutrophil cytoplasmic antibody associated with systemic disease characterized by granulomas and vasculitis affecting small and medium vessels. Neurological manifestations in GPA are less frequent than classical manifestations, such as lung and kidney involvement, and cranial nerve palsies are much rarer. Cyclophosphamide and glucocorticoids have been conventionally administered as an initial induction immunosuppressive therapy for GPA. However, increasing evidence has demonstrated the efficacy and safety of rituximab, an anti-B cell monoclonal antibody, for the treatment of GPA. Herein, we describe a successful treatment of relapsing GPA with cranial nerve involvement using rituximab in a 56-year-old male patient who was previously treated with cyclophosphamide plus glucocorticoids.
Antibodies, Antineutrophil Cytoplasmic
;
Cranial Nerve Diseases
;
Cranial Nerves
;
Cyclophosphamide
;
Glucocorticoids
;
Granuloma
;
Humans
;
Kidney
;
Lung
;
Male
;
Middle Aged
;
Neurologic Manifestations
;
Vasculitis
;
Rituximab
6.A Case of Squamoid Cyst of Pancreatic Ducts Shown Malignant Behavior.
Yoon Seok CHOI ; Tae hyun BAN ; Ik Hyun JO ; Ji Young MOK ; Sang Bin LEE ; Won Jik LEE ; Sung Hoon JUNG ; Jung Hwan OH
Korean Journal of Pancreas and Biliary Tract 2014;19(2):84-89
Detection of cystic lesions in the pancreas has increased because of the widespread use of high-resolution diagnostic imaging techniques. Therefore, cystic lesions of the pancreas constitute an increasingly important category with a challenging differential diagnosis. Squamoid cyst of pancreatic ducts is a recently recognized type of cystic lesion in the pancreas in which cystically dilated ducts are lined by non-keratinized squamous epithelium. Although it is clinically known as benign cystic lesion, we experienced its malignant behavior and report here with review of the international literatures.
Diagnosis, Differential
;
Diagnostic Imaging
;
Epithelium
;
Pancreas
;
Pancreatic Cyst
;
Pancreatic Ducts*
;
Pancreatic Neoplasms
7.The Potential Utility of Iodinated Contrast Media (ICM) Skin Testing in Patients with ICM Hypersensitivity.
Young Hwan AHN ; Young Il KOH ; Joo Hee KIM ; Ga Young BAN ; Yeon Kyung LEE ; Ga Na HONG ; U Ram JIN ; Byung Joo CHOI ; Yoo Seob SHIN ; Hae Sim PARK ; Young Min YE
Journal of Korean Medical Science 2015;30(3):245-251
Both immediate and delayed hypersensitivity reactions to iodinated contrast media (ICM) are relatively common. However, there are few data to determine the clinical utility of immunologic evaluation of ICM. To evaluate the utility of ICM skin testing in patients with ICM hypersensitivity, 23 patients (17 immediate and 6 delayed reactions) were enrolled from 3 university hospitals in Korea. With 6 commonly used ICM including iopromide, iohexol, ioversol, iomeprol, iopamidol and iodixanol, skin prick (SPT), intradermal (IDT) and patch tests were performed. Of 10 patients with anaphylaxis, 3 (30.0%) and 6 (60.0%) were positive respectively on SPTs and IDTs with the culprit ICM. Three of 6 patients with urticaria showed positive IDTs. In total, 11 (64.7%) had positive on either SPT or IDT. Three of 6 patients with delayed rashes had positive response to patch test and/or delayed IDT. Among 5 patients (3 anaphylaxis, 1 urticaria and 1 delayed rash) taken subsequent radiological examinations, 3 patients administered safe alternatives according to the results of skin testing had no adverse reaction. However, anaphylaxis developed in the other 2 patients administered the culprit ICM again. With 64.7% (11/17) and 50% (3/6) of the sensitivities of corresponding allergic skin tests with culprit ICM for immediate and delayed hypersensitivity reactions, the present study suggests that skin tests is useful for the diagnosis of ICM hypersensitivity and for selecting safe ICM and preventing a recurrence of anaphylaxis caused by the same ICM.
Anaphylaxis/chemically induced/diagnosis/immunology
;
Contrast Media/*adverse effects
;
Cross Reactions/immunology
;
Dermatitis, Contact/*diagnosis/*immunology
;
Drug Hypersensitivity/diagnosis
;
Female
;
Humans
;
Iodides/*immunology
;
Iohexol/analogs & derivatives
;
Iopamidol/analogs & derivatives
;
Male
;
Middle Aged
;
Republic of Korea
;
Skin Tests/*methods
;
Triiodobenzoic Acids
;
Urticaria/diagnosis/immunology
8.Living Donor Liver Transplantation in a Korean Child with Glycogen Storage Disease Type IV and a GBE1 Mutation.
Hye Ryun BAN ; Kyung Mo KIM ; Joo Young JANG ; Gu Hwan KIM ; Han Wook YOU ; Kyungeun KIM ; Eunsil YU ; Dae Yeon KIM ; Ki Hun KIM ; Young Joo LEE ; Sung Gyu LEE ; Young Nyun PARK ; Hong KOH ; Ki Sup CHUNG
Gut and Liver 2009;3(1):60-63
Glycogen storage disease type IV (GSD-IV) is an autosomal recessive disease caused by a deficient glycogen branching enzyme (GBE), encoded by the GBE1 gene, resulting in the accumulation of abnormal glycogen deposits in the liver and other tissues. We treated a 20-month-old girl who presented with progressive liver cirrhosis and was diagnosed with GSD-IV, as confirmed by GBE1 gene mutation analysis, and underwent living related heterozygous donor liver transplantation. Direct sequencing of the GBE1 gene revealed that the patient was compound heterozygous for a known c.1571G>A (p.Gly264Glu) mutation a novel c.791G> A (Arg524Gln) mutation. This is the first report of a Korean patient with GSD-IV confirmed by mutation analysis, who was treated successfully by liver transplantation.
1,4-alpha-Glucan Branching Enzyme
;
Child
;
Glycogen
;
Glycogen Storage Disease
;
Glycogen Storage Disease Type IV
;
Humans
;
Infant
;
Liver
;
Liver Cirrhosis
;
Liver Transplantation
;
Living Donors
;
Tissue Donors
9.A Case of Pulmonary Paragonimiasis with Recurrent Pleural Effusion and Cured by Four Cycles of Chemotherapy.
Gye Jung CHO ; Jung Hwan LIM ; Dong Ryeol CHAE ; Su Young CHI ; Hee Jung BAN ; Byeong Kab YOON ; Yong Soo KWON ; In Jae OH ; Kyu Sik KIM ; Yu Il KIM ; Sung Chul LIM ; Young Chul KIM
Tuberculosis and Respiratory Diseases 2009;66(6):451-456
Paragonimiasis is a parasitic infection that occurs following the ingestion of infectious Paragonimus metacercariae, which occurs as a result of eating raw or undercooked freshwater crabs or crayfish. Pulmonary paragonimiasis is the most common clinical manifestation of this infection. Human paragonimiasis occurs sporadically. We experienced a case of pulmonary paragonimiasis in a 35-year-old woman with left lower chest pain. The patient had hypereosinophilia and a pleural effusion. The diagnosis was confirmed by positive ELISA (Enzyme-linked immunosorbent assay) that detected Paragonimiasis westermani antibody in the serum. We treated the patient with praziquantel for two days at a daily dosage of 75 mg/kg. Left pleuritic pain and pleural effusion improved after treatment. However, similar symptoms and pleural effusion developed recurrently for the first 3 courses of treatment with praziquantel. Upon the fourth round of treatment, the patient made a full recovery.
Adult
;
Astacoidea
;
Chest Pain
;
Eating
;
Enzyme-Linked Immunosorbent Assay
;
Female
;
Fresh Water
;
Humans
;
Metacercariae
;
Paragonimiasis
;
Paragonimus
;
Pleural Effusion
;
Praziquantel
;
Recurrence
10.Anti-atherosclerotic effects of perilla oil in rabbits fed a high-cholesterol diet.
Yeseul CHA ; Ja Young JANG ; Young Hwan BAN ; Haiyu GUO ; Kyungha SHIN ; Tae Su KIM ; Sung Pyo LEE ; Jieun CHOI ; Eun Suk AN ; Da Woom SEO ; Jung Min YON ; Ehn Kyoung CHOI ; Yun Bae KIM
Laboratory Animal Research 2016;32(3):171-179
Anti-atherosclerosis effects of perilla oil were investigated, in comparison with lovastatin, in rabbits fed a high-cholesterol diet (HCD). Hypercholesterolemia was induced in rabbits by feeding the HCD containing 0.5% cholesterol and 1% corn oil, and perilla oil (0.1 or 0.3%) was added to the diet containing 0.5% cholesterol for 10 weeks. HCD greatly increased blood total cholesterol and low-density lipoproteins, and caused thick atheromatous plaques, covering 74% of the aortic wall. Hyper-cholesterolemia also induced lipid accumulation in the liver and kidneys, leading to lipid peroxidation. Perilla oil not only attenuated hypercholesterolemia and atheroma formation, but also reduced fat accumulation and lipid peroxidation in hepatic and renal tissues. The results indicate that perilla oil prevents atherosclerosis and fatty liver by controlling lipid metabolism, and that it could be the first choice oil to improve diet-induced metabolic syndrome.
Atherosclerosis
;
Cholesterol
;
Corn Oil
;
Diet*
;
Fatty Liver
;
Hypercholesterolemia
;
Kidney
;
Lipid Metabolism
;
Lipid Peroxidation
;
Lipoproteins, LDL
;
Liver
;
Lovastatin
;
Perilla*
;
Plaque, Atherosclerotic
;
Rabbits*