OBJECTIVES: LOINC(R)(Logical Observations Identifiers, Names, Codes) is being used as the global standard for sharing laboratory test information and standardization. However, difficulties have been encountered in transferring local code to LOINC. Use in existing laboratory information systems(LIS) is possible with maximized local codes and LOINC mapping. Since the existing mapping tool has parts that do not match domestic medical environments, it is difficult to use without modification or supplementation. To this end, we have developed algorithms for LOINC mapping and have evaluated their usefulness. METHODS: We used 2,376 M-codes transformed from Pusan National University Hospital's 1,150 local codes, and codes from various laboratory test domains(Diagnostic Hematology, Clinical Chemistry, Seroimmunology, Molecular and Cytogenetics, Microbiology, Transfusion Medicine). In materializing the automatic mapping algorithms, spread sheet programs(Excel, Microsoft) and existing mapping tools(RELMA, Regenstrief) were used. The accuracy of the mapped codes was verified by a specialist of the Laboratory Medicine Department. RESULTS: Of the 2,376 M-codes, mapping on LOINC was found to be possible for 78.7%(1,871) while LOINC corresponding with the local codes could not be found for 21.3%(505). Of the mapped codes, 90.8%(1,699) were mapped accurately automatically, while the rest were mapped manually. CONCLUSIONS: The LOINC mapping algorithm that was developed in this study was useful for mapping various forms of local code with LOINC.
Adoption ; Chemistry, Clinical ; Cytogenetics ; Hematology ; Logical Observation Identifiers Names and Codes ; Specialization

The developmental topography of olivocerebellar projection is not fully understood. Insulin-like growth factor-I (IGF-I) plays important roles in neural development. This study examined to observe IGF-I-like immunoreactivity (IGF-I IR) in the cerebellum and inferior olive of postnatal developing and adult rats. IGF-I immunoreactive Purkinje cells exhibited spatially and temporally regulated distribution which correlates with climbing fiber development. At birth a few IGF-I immunoreactive Purkinje cells were stained weakly only in the ventral vermis. By P7, all Purkinje cells of the vermis and hemispheres were positively labelled. A subpopulation of Purkinje cells lost IGF-I IR, and IGF-I IR Purkinje cells were divided into discrete population arranged in sagittal strips which were separated by non-reactive Purkinje cells. In the inferior olive, neurons showed IGF-I IR between P0 and P7. By P10 the inferior olive neurons were all negative for IGF-IR and this was mantained to adulthood. The IGF-I IR for Purkinje cell and inferior olive coincides with climbing fiber development and thus the results of this study support the hypothesis that IGF-I is specially involved in the refinement of olivocerebellar topography during synaptogenesis.
Adult* ; Animals ; Cerebellum ; Humans ; Insulin-Like Growth Factor I ; Neurons ; Olea ; Parturition ; Purkinje Cells ; Rats*

Interstitial cells of Cajal (ICC) are the pacemakers in gastrointestinal slow wave, and also transduce signal inputs from the enteric nervous system to smooth muscle. The abnormal motility corresponded to a lack or decreasing of ICC and a disruption of electrical slow waves. So we developed partial obstruction model in murine small intestine and investigated changes in the ICC networks and electrical activity in the obstructed bowel using c-kit immunohistochemistry and intracelluar electrophysiological techniques. Two weeks following the onset of a partial obstruction, the small intestine increased in diameter and muscular hypertrophy was developed oral to the obstruction site. ICC were absent or only weak at 1 ~25 mm oral to the occlusion site, and this disruption was accompanied by the loss of electrical slow wave. ICC networks and slow waves were normal appearance aboral to the clip. In conclusion, The present results showed that partial intestinal obstruction induced the loss of ICC networks and slow waves. These result will provide a valuable aid for understanding pathogenesis of intestinal motility disorder, and this model may be an important tool for evaluating genetic or molecular factor for the therapeutic opportunities of motility disorder in human.
Animals ; Enteric Nervous System ; Gastrointestinal Motility* ; Humans ; Hypertrophy ; Immunohistochemistry ; Interstitial Cells of Cajal* ; Intestinal Obstruction ; Intestine, Small ; Mice ; Muscle, Smooth