Primary ovarian transitional cell carcinoma is extremely rare tumor. The histologic subtype was divided from malignant Brenner tumor due to it's own histologic characteristics and chemosensitive nature. Most of recent studies revealed that transitional cell carcinoma has a good response to chemotherapy and long-term survival. Recent histopathologic reports show that transitional cell carcinoma of the bladder and of the ovary are immunophenotypically different. We experienced a case of primary ovarian transitional cell carcinoma, and report this case with a brief review of the concerned literatures.
Brenner Tumor ; Carcinoma, Transitional Cell* ; Drug Therapy ; Female ; Ovary ; Urinary Bladder

Murine norovirus (MNV) is a non-enveloped virus with a positive-sense RNA genome and causes lethal infection in mice. MNV has been used as a model virus for human norovirus (NV) whose in vitro cell culture system has not been available to date since MNV and NV are genetically related. In this study, the genome replication of MNV was investigated using strand-specific RT-PCR in RAW264.7 cells. Reverse transcription (RT) using a sense primer followed by PCR showed that negative-sense RNAs were first detected in RAW264.7 cells between 6 and 9 [3 and 6] hours post infection (h.p.i.). However, these negative-sense RNAs were not detected when cells were treated with a translation inhibitor cycloheximide. Then, RT with an antisense primer followed by PCR was performed to detect positive-sense RNAs. RT-PCR results revealed that the amount of positive-sense RNAs began to increase from 9 [6] h.p.i., indicating the accumulation of the newly synthesized (+)RNA genome. Furthermore, cycloheximide abrogated the increase of newly made RNAs during MNV infection. In conclusion, strand-specific RT-PCR using a sense or antisense primer, in combination with cycloheximide treatment, enabled us to detect positive-sense and negative-sense RNAs selectively and provided a useful tool to understand the replication cycle of MNV.
Animals ; Cell Culture Techniques ; Cycloheximide ; Genome ; Humans ; Mice ; Norovirus ; Polymerase Chain Reaction ; Reverse Transcription ; RNA ; Viruses

BACKGROUND: The recurrence rate of rosacea was not known very well, but has been reported as 60% in 6 months after withdrawal of the drug. It is not known which treatment can reduce relapses of rosacea effectively. OBJECTIVE: The objective was to identify whether 595 nm-pulsed dye laser (PDL) treatment reduced recurrence rate among rosacea patients who were treated with oral minocycline. METHODS: One hundred and seven Korean patients with rosacea who started treatment with oral minocycline (100 mg/d) with or without PDL (2∼4 sessions) were evaluated retrospectively. The recurrence rate was estimated using the Kaplan-Meier method, and difference was evaluated using the log-rank test. Cox proportional hazards model was used to estimate hazard ratios and 95% confidence intervals (CIs) of risk factors for the recurrence of rosacea. RESULTS: The recurrence-free survival analysis revealed that the group with oral minocycline plus PDL was significantly different compared with the group with oral minocycline alone (p=0.011). Cox proportional hazards model showed that the combined use of PDL with oral minocycline appeared to be a significant protective factor for the hazard of recurrence of rosacea (hazard ratio, 0.492; 95% CI, 0.257∼0.941; p=0.032). CONCLUSION: PDL can be used added to oral minocycline to reduce relapses among rosacea patients who are undergoing oral minocycline treatment.
Humans ; Lasers, Dye* ; Methods ; Minocycline* ; Proportional Hazards Models ; Protective Factors ; Recurrence* ; Retrospective Studies ; Risk Factors ; Rosacea*

PURPOSE: This study aimed to investigate the relationship among nursing professionalism, perfectionism, resilience, and burnout, amongst nurses in cancer wards, and to further identify factors influencing burnout. METHODS: Conducted in June 2018, this descriptive cross-sectional study included 157 cancer wards nurses. The survey employed structured questionnaires including the Koreannursing Professional Value Scale, Multidimensional Perfectionism Scale, Dispositional Resilience Scale-15, and burnout subscale of the Professional Quality of Life Scale. Data were analyzed using descriptive statistics, independent t-tests, one-way ANOVA, Pearson's correlation coefficient, and entered multiple regression. RESULTS: The total burnout score was 31.20±4.87, out of a maximum of 50. Nursing professionalism (r =−.40, p < .001) and resilience (r=−.68, p < .001) showed a negative correlation with burnout, while socially-oriented perfectionism showed a positive correlation with burnout (r=.19, p=.016). Entered multiple regression revealed that 49.3% of the total variance in burnout was the consequence of nursing professionalism and resilience. CONCLUSION: Resilience had the largest effect on burnout, followed by nursing professionalism. We believe that the results of this study provide basic data for developing nursing intervention programs aimed at reducing burnout amongst nurses in cancer wards.
Cross-Sectional Studies ; Nursing* ; Professionalism* ; Quality of Life

Background and Objectives: Whether beta blockers favorably impact the clinical outcome in patients with acute myocardial infarction (AMI) remains in debate. We investigated the impact of beta blocker on major clinical outcomes during 2 years after percutaneous coronary intervention (PCI) in patients with AMI. Methods: All patients with the first AMI treated with PCI for the period of 2005 to 2014 from the Korean National Health Insurance Service claims database were enrolled. We defined the regular user as medication possession ratio (MPR) ≥80% and non-user as MPR=0%. We compared the occurrence of all cause death, myocardial infarction (MI) and stroke according to adherence of beta-blockers. A 1:1 propensity score-matching was conducted to adjust for between-group differences. Results: We identified a total 81,752 patients with met eligible criteria. At discharge, 63,885 (78%) patients were prescribed beta blockers. For 2 years follow up period, regular users were 53,991 (66%) patients, non-users were 10,991 (13%). In the propensity score matched population, regular use of beta blocker was associated with a 36% reduced risk of composite adverse events (all death, MI or stroke) (hazard ratio [HR], 0.636; 95% confidence interval [CI], 0.555–0.728; p<0.001). Compared to no use of beta blocker, regular use significantly reduced all death (HR, 0.736; 95% CI, 0.668–0.812; p<0.001), MI (HR, 0.729; 95% CI, 0.611–0.803; p<0.001) and stroke (HR, 0.717; 95% CI, 0.650–0.791; p<0.001). Conclusions Prescription of beta blocker in patients with AMI after PCI was sequentially increased. Continuous regular use of beta blocker for 2 years after AMI reduced major adverse events compared to no use of beta blocker.

BACKGROUND AND OBJECTIVES: Genomic imprinting modulates growth and development in mammals and is associated with genetic disorders. Although uniparental embryonic stem cells have been used to study genomic imprinting, there is an ethical issue associated with the destruction of human embryos. In this study, to investigate the genomic imprinting status in human neurodevelopment, we used human uniparental induced pluripotent stem cells (iPSCs) that possessed only maternal alleles and differentiated into neural cell lineages. METHODS: Human somatic iPSCs (hSiPSCs) and human parthenogenetic iPSCs (hPgiPSCs) were differentiated into neural stem cells (NSCs) and named hSi-NSCs and hPgi-NSCs respectively. DNA methylation and gene expression of imprinted genes related neurodevelopment was analyzed during reprogramming and neural lineage differentiation. RESULTS: The DNA methylation and expression of imprinted genes were altered or maintained after differentiation into NSCs. The imprinting status in NSCs were maintained after terminal differentiation into neurons and astrocytes. In contrast, gene expression was differentially presented in a cell type-specific manner. CONCLUSIONS: This study suggests that genomic imprinting should be determined in each neural cell type because the genomic imprinting status can differ in a cell type-specific manner. In addition, the in vitro model established in this study would be useful for verifying the epigenetic alteration of imprinted genes which can be differentially changed during neurodevelopment in human and for screening novel imprinted genes related to neurodevelopment. Moreover, the confirmed genomic imprinting status could be used to find out an abnormal genomic imprinting status of imprinted genes related with neurogenetic disorders according to uniparental genotypes.
Alleles ; Astrocytes ; Cell Lineage ; DNA Methylation ; Embryonic Stem Cells ; Embryonic Structures ; Epigenomics ; Ethics ; Gene Expression ; Genomic Imprinting ; Genotype ; Growth and Development ; Humans ; In Vitro Techniques ; Induced Pluripotent Stem Cells ; Mammals ; Mass Screening ; Neural Stem Cells ; Neurons

PURPOSE: To determine the usefulness of perfusion weighted MR imaging in the assessment of relative cerebral blood volume(rCBV) in brain tumors. MATERIALS AND METHODS: Twenty-three patients with primary or metastatic brain tumors [nine gliomas (6 high grade and 3 low), six metastatic tumors, five meningiomas (4 benign and 1 atypical), two neurilemmomas and one hemangioblastoma] underwent perfusion-weighted and conventional MR imaging. A total of 240 perfusion MR images were obtained from four axial slices after rapid injection of contrast media using a gradient echo planar imaging pulse sequence, and this was followed by postprocessing of these images to give CBV maps. In order to calculate the rCBV of tumor to normal white matter, ROIs were defined on the CBV map of a tumor and its contralateral normal white matter. RESULTS: The rCBV ratio of tumors to contralateral normal side was as follows: high-grade glioma, 0.40-5.64(mean +/-SD = 2.91 0.95); low grade astrocytoma, 0.77 -1.66 (mean +/-SD = 1.15 +/-0.28); benign menin-gioma,2.06 -4.90 (mean +/-SD = 3.59 +/-0.84); atypical meningioma, 0.46 -1.18 (mean +/-SD = 0.72 +/-0.25); neurilemmoma, 1.45 -3.85 (mean +/-SD = 2.56 +/-0.92); and hemangioblastoma, 6.16 -8.35 (mean +/-SD = 7.02 +/-1.12). High grade gliomas were more hypervascular than low grade astrocytomas, and showed a variable range of relative cerebral blood volume. In metaststic cancer, CBV maps showed a relatively high and variable blood volume. Benign meningiomas exhibited high relative cerebral blood volume, while in the atypical meningioma with cystic degeneration, this volume was low. In neurilemmomas, a variable range of relative cerebral blood volume, was noted, while in the mural nodule of the hemangioblastoma, this volume was the highest. CONCLUSION: Perfusion-weighted MRI indicated the rCBV of various brain tumor lesions, and this suggests that the modality can provide a very useful means of assessing brain tumor vascularity.
Astrocytoma ; Blood Volume ; Brain Neoplasms* ; Brain* ; Contrast Media ; Echo-Planar Imaging ; Glioma ; Hemangioblastoma ; Humans ; Magnetic Resonance Imaging* ; Meningioma ; Neurilemmoma ; Perfusion*

Drug-eluting stents (DES) are now widely used for patients with coronary artery disease undergoing percutaneous coronary interventions. The current major agenda for using DES is very late stent thrombosis (VLST) that occurs beyond 1 year after DES implantation. Although VLST is rare, it is a serious complication that can result in sudden death or myocardial infarction. Until now, there have been only a few case reports of VLST within 7 years. We report a case of a 78-year-old man who presented with an ST segment elevation myocardial infarction due to extremely very late stent thrombosis resulting from a mal-apposed stent and delayed neointimal coverage that occurred 8 years after stent implantation after the cessation of antiplatelet agents for 10 days.
Aged ; Coronary Artery Disease ; Death, Sudden ; Drug-Eluting Stents* ; Humans ; Myocardial Infarction ; Percutaneous Coronary Intervention ; Platelet Aggregation Inhibitors ; Stents* ; Thrombosis* ; Ultrasonography*

PURPOSE: The use of mechanically-assisted ventilators at home reduces morbidity and improves the quality of life in children with chronic respiratory failure. But in Korea there is no clinical data of children with home mechanical ventilation. We investigated ventilator types, duration, the causes of failure or death, and the cost needed for care. METHODS: We retrospectively analyzed the medical records of 21 children who were admitted and who applied for home mechanical ventilation at the Pediatric Intensive Care Unit in Asan Medical Center. Phone interviews took place after discharge. and interviewed by phone after discharge. RESULTS: The median age was 31 months; the median duration with ventilator was 25 months. Underlying diseases were 16 neuromuscular diseases, one metabolic disease and four chronic respiratory diseases. The types of ventilator were pressure and volume type(16 and five patients, respectively). The frequency of ventilation failure was once per 19 months. Weaning could be performed in three cases. Frequencies of admission after receiving ventilators were 1.7 times per year; the most common cause was pneumonia. Nine patients(43%) died; four of them died because of endotracheal tube obstruction. The costs for medical care were about 1,110,000 won per month. CONCLUSION: There is an increment in the numbers of individuals who need mechanical ventilation support. The most common cause of death was endotracheal tube obstruction. The most important problem for the patients was medical cost. There needs to be more interest in patients with ventilator and social welfare systems to support their families need to be prepared.
Cause of Death ; Child* ; Chungcheongnam-do ; Humans ; Intensive Care Units ; Korea ; Medical Records ; Metabolic Diseases ; Neuromuscular Diseases ; Pneumonia ; Quality of Life ; Respiration, Artificial* ; Respiratory Insufficiency ; Retrospective Studies ; Social Welfare ; Ventilation ; Ventilators, Mechanical ; Weaning

PURPOSE: Paraquat is widely used non-selective contact herbicide. In spite of efforts to improve the outcome in paraquat poisoning, the mortality rate still remains high. The purpose of this study is to assess the predictive factors of prognosis by investigating initial laboratory data on paraquat poisoning. METHODS: The author retrospectively analyzed the clinical features, laboratory data, and outcomes for 130 paraquatpoisoning patients treated from June 1997 to September 2003 at the emergency medical center of Chonnam National University Hospital. RESULTS: The results were as follows: 1) The mortalities were significantly older than the survivors (49.3 years vs 37.6 years, p<0.05). Among the total 130 cases, the survivors numbered 50, and the deceased numbered 80. The mean age of all 130 cases was 44.8+/-16.9 years. 2) The white blood cell count and the levels of serum AST and ALT in the deceased were significantly higher than they were in the survivors. The levels of arterial pH, PaCO 2, base excess, HCO3 -, and serum potassium in the survivors were significantly higher than they were in the deceased. 3) A multivariate Cox-Regression analysis revealed that sex, serum potassium, and arterial HCO3 - were associated with the mortality rate. CONCILUSION: The above data reveal that the ingested amount, the result of the urine sodium dithionite test, and the initial state of laboratory parameters, including the white blood cell count, ABGA (pH, PaCO2, HCO3 -, base excess), serum potassium, AST, and ALT, can be used to predict the outcome of paraquat poisoning.
Dithionite ; Emergencies ; Humans ; Hydrogen-Ion Concentration ; Jeollanam-do ; Leukocyte Count ; Mortality ; Paraquat* ; Poisoning* ; Potassium ; Prognosis* ; Retrospective Studies ; Survivors