To localize glycoconjugates of surface mucous cells, mucous neck cells and chief cells in the developing rat, nine of biotinylated lectin(SBA, DBA, PNA, BSL-1, RCA-1, sWGA, UEA-1, Con A and LCA) were applied with ABC method. In the surface and gastric pit epithelium of body of the stomach, DBA affinity was not demonstrated. Although Con A and LCA affinity were slightly increased after birth, these affinities with RCA-1 and sWGA maintained constantly from fetal to adult rat. And UEA-1 affinity gradually increased from the end of suckling period. BSL-1 and PNA affinity showed a tendency to decrease and was not observed in most cells from the suckling and weanling period respectively. In the gastric gland proper, mucous neck cells and chief cells were not distinguished until the early weanling period. All affinities examined except DBA and BSL-1 were observed and increased in the gland of postnatal rat. With the approach of weanling period, more intense affinity for PNA, RCA-1, sWGA and UEA-1 were found on lower portion of the gastric gland proper and more intense affinity for SBA on upper portion. The mucous neck cells showed a similar affinities as gastric gland proper from the weanling period and two affinities for PNA and Con A were detected in the chief cell.
Adult ; Animals ; Epithelium ; Gastric Mucosa* ; Glycoconjugates ; Humans ; Neck* ; Parturition ; Rats* ; Stomach

The distribution of transforming growth factor -alpha (TGF -alpha ) and epidermal growth factor (EGF) on the cardiovascular system of developing mouse embryos of gestational age 7 to 12 days were immunohistochemically (ABC method) studies to investigate the differential expression of these growth factors. Paraffin embedded sections were immunostained with antibodies for TGF -alpha and EGF. In the 8 -day -old mouse embryos, the endocardial tissue, myocardial tissue and cardiac jelly were all TGF -alpha stained. EGF stain was observed in the cardiac jelly and myocardial tissue but was not observed in the endocardial tissue. This suggests that in the initial phase of the cardiovascular system development, TGF -alpha function as earlier growth factor than EGF. The 9, 10 and 11 -day -old embryos showed TGF -alpha stain in the broad spectrum of developing cardiovascular tissues such as, the bulbus cordis, primitive atrium, sinus venosus, aortic sac, dorsal aorta, vitelline artery, endocardial cushion tissue, and myocardium of primitive ventricle. However, EGF stain was observed only in the bulbus cordis, primitive atrium and endocardial tissue. This finding indicates that TGF -alpha function as a more extensive growth factor than EGF. The 12 -day -old embryos showed stronger EGF stain than TGF -alpha in the primitive ventricle, bulbus cordis, and endocardial tissue. This suggests that EGF function as a more growth factor than TGF -alpha at this particular developmental stage and plays important role at the end stage of the primitive heart development.
Animals ; Antibodies ; Aorta ; Arteries ; Cardiovascular System ; Embryonic Structures* ; Endocardial Cushions ; Epidermal Growth Factor* ; Gestational Age ; Heart ; Immunohistochemistry ; Intercellular Signaling Peptides and Proteins ; Mice* ; Myocardium ; Paraffin ; Transforming Growth Factors* ; Vitellins

To examine the effects of electroacupuncture (EA) on central nociceptive modulation, expressional changes of spinal neuronal nitric oxide synthase (nNOS) were investigated in complete Freund's adjuvant (CFA)-injected rats. Inflammation was induced by an intraplantar injection of CFA into the hindpaw of male Sprague-Dawley rats. Bilateral EA stimulation at 2 Hz, 15 Hz and 120 Hz was applied at those acupoints corresponding to Zusanli and Sanyinjiao in man with 3-day intervals for 30 days. At 30 days after CFA-injection, effects of EA on nNOS expression were observed in the dorsal horn of the spinal cord using immunohistochemical methods. The mean integrated optical density of nNOS immunoreaction was significantly increased in the dorsal horn throughout L1 to L5 lumbar segments in CFA-injected rats. The nNOS expression was attenuated in all regions of the dorsal horn by all types of EA. Especially, these reaction was markedly decreased in the superficial laminae and nucleus proprius of L1 and L3 lumbar segments by three types of EA, but a marked decrease in the neck of the dorsal horn was observed only in 2 Hz stimulation. The marked decrease of nNOS also showed in nucleus proprius and the neck of L5 lumbar segments in 2 Hz and 15 Hz EA stimulated rats. It is concluded that EA treatment can attenuate chronic inflammatory process in CFA-injected rats through modulating expression of nNOS in the dorsal horn of the spinal cord.
Acupuncture Points ; Animals ; Electroacupuncture* ; Freund's Adjuvant ; Horns ; Humans ; Inflammation ; Male ; Models, Animal* ; Neck ; Neurons* ; Nitric Oxide Synthase ; Nitric Oxide Synthase Type I* ; Rats* ; Rats, Sprague-Dawley ; Spinal Cord

Upward migration of the peritoneal catheter of a subgaleo-peritoneal (SP) shunt and coiling into the subgaleal space is an extremely rare complication of a SP shunt. A 32-year-old male patient visited our hospital presenting with a large skull defect due to a prior craniectomy performed elsewhere. The patient underwent a cranioplasty with methylmetacrylate, but subsequently developed progressive pseudomeningocele and subgaleal cerebrospinal fluid (CSF) collection. The patient underwent CSF diversion via a SP shunt. After SP shunting, the pseudomeningocele disappeared completely. Six months later, the patient presented with progressive scalp swelling. Skull X-ray showed migration and coiling of the distal catheter of the SP shunt. The patient was treated by removing the entire shunt catheter and the dura was covered with a subgaleal flap. We would like to report our experience with a very rare complication of subgaleo-peritoneal shunting.
Adult ; Catheters* ; Cerebrospinal Fluid ; Humans ; Male ; Scalp ; Skull

Purpose: In this paper, we propose deep-learning methodology with which to enhance the mass differentiation performance of convolutional neural network (CNN)-based architecture. Materials and Methods: We differentiated breast mass lesions from gray-scale X-ray mammography images based on regions of interest (ROIs). Our dataset comprised breast mammogram images for 150 cases of malignant masses from which we extracted the mass ROI, and we composed a CNN-based deep learning model trained on this dataset to identify ROI mass lesions. The test dataset was created by shifting some of the training data images. Thus, although both datasets were different, they retained a deep structural similarity. We then applied our trained deep-learning model to detect masses on 8-bit mammogram images containing malignant masses. The input images were preprocessed by applying a scaling parameter of intensity before being used to train the CNN model for mass differentiation. Results: The highest area under the receiver operating characteristic curve was 0.897 (Î 20). Conclusion Our results indicated that the proposed patch-wise detection method can be utilized as a mass detection and segmentation tool.

Failure of normal embryogenesis may result in various anomalies of the inferior vena cava that can bereli-ably detected by computed tomography. Agenesis of the intrahepatic segment of the inferior vena cava withazygos continuation is a rare anomaly due to a complex developmental process of the inferior vena cava. Theauthors report a case in which this anomaly was diagnosed on computed tomography and confirmed by inferi-or venacavography.
Azygos Vein ; Embryonic Development ; Female ; Pregnancy ; Vena Cava, Inferior*

Between March 1994 and March 1995, 10 patients with thoracolumbar burst fractures underwent a one-stage operation consisting of anterior decompression, reduction, bony fusion with iliac bone and stabilization with Kaneda device. The mean follow-up was 6.4 Months. Most patients with incomplete neurologic lesions showed postoperative improvement and were upgraded one or two steps in the Frankel scale. No patient showed neurological deterioration after surgery. Loss of reduction was 5.5 degree during follow-up period. Anterior decompression and strut fusion was used to effectively recover the neurological deficit and reduce the pain in a thoraco-lumbar burst fracture.
Decompression ; Follow-Up Studies ; Humans

Inducible nitric oxide synthase (iNOS) expression of several organs on the lipopolysaccharides (LPS)-injected rats and on excisional wound was observed by immunohistochemical methods to investigate iNOS-positive cells during inflammation. iNOS expression was induced in response to LPS in the brain and these reactions were observed in the choroidal epithelium, ependymal cells and a few of nerve cells and fiber. A more intensive reaction of nerve cell and fiber was mainly observed in the corpus callosum and hypothalamus. Induction of iNOS of the lung was observed in alveolar macrophage, smooth muscle, pneumocytes and inflammatory cells infilterated in the alveolar septum. iNOS expression of the liver was detected in Kupffer cells, hepatocytes, bile duct and inflammatory cells of spotty necrosis. The cardiac muscle and endothelial cell of the heart showed positive iNOS expression. In the excisional wound, inflammatory cells including macrophages, neutrophil and fibrobast showed iNOS expression and mainly detected necrobiotic layer. Collectively, iNOS expression was induced in the several cell types during inflammatory process. So for better understanding the function of iNOS, more research should be done in relation to each cell type of organ.
Animals ; Bile Ducts ; Brain ; Choroid ; Corpus Callosum ; Endothelial Cells ; Epithelium ; Heart ; Hepatocytes ; Hypothalamus ; Inflammation ; Kupffer Cells ; Lipopolysaccharides ; Liver ; Lung ; Macrophages ; Macrophages, Alveolar ; Muscle, Smooth ; Myocardium ; Necrosis ; Neurons ; Neutrophils ; Nitric Oxide Synthase Type II* ; Pneumocytes ; Rats* ; Shock* ; Wound Healing* ; Wounds and Injuries*

We report a case of irreducible atlantaxial dislocation with spinal cord injury. The patient was a 36-year-old man who suffered from the sudden onset of shallow respiration and severe motor weakness after slip down. We have performed the one-stage operation, the transoral decompression, reduction and posterior interspinous wiring with bone fusion. Three weeks after surgery, the reduction state and posterior wiring procedure were failed, due to severe osteoporotic change. So, we have performed second operation, the transarticular facet screw fixation and occipito-cervical fixation with Steinmann pin, from which good postoperative stability and neurological improvements, were obtained.
Adult ; Decompression* ; Dislocations* ; Humans ; Osteoporosis ; Respiration ; Spinal Cord Injuries* ; Spinal Cord*

PURPOSE: To compare the accuracy and clinical usefulness of different devices by measuring anterior chamber depth (ACD) with three devices and central corneal thickness (CCT) with four devices. METHODS: In 180 eyes of 90 healthy subjects, ACD was measured using A-scan, Lenstar LS900®, Pentacam®, and CCT was measured using ultrasound pachymetry (USP), Lenstar LS900®, Pentacam®, and anterior segment optical coherence tomography (OCT). RESULTS: The average ACT measurements using Lenstar LS900®, A-scan, and Pentacam® were 3.27 ± 0.35 mm, 3.26 ± 0.36 mm, and 3.25 ± 0.36 mm, respectively. The measurements were significantly correlated (p < 0.001) but without statistically significant difference (p = 0.017). The Bland-Altman plots showed a low degree of agreement. The average CCT measurements using Pentacam®, USP, Lenstar LS900®, and OCT were 553.31 ± 25.23 µm, 547.26 ± 23.83 µm, 541.38 ± 24.49 µm, and 531.40 ± 22.33 µm, respectively. The measurements were significantly correlated (p < 0.001) and statistically significantly different (p < 0.05). The Bland-Altman plots showed a low degree of agreement. CONCLUSIONS: ACD and CCT measured using different devices were highly correlated, but the ACD measurements were not statistically different; however, the CCT measurements were statistically different, and agreement was low between both measurements.
Anterior Chamber* ; Healthy Volunteers ; Tomography, Optical Coherence ; Ultrasonography