In 1927, Hamperl carried out histochemical studies on gastric cancer and found one case which contained many Fontana reactive argentaffin cells. Thereafter the gastric and intestinal adenocarcinomas which contain enterochromaffin cells such as argentaffin cell and argyrophil cell have been reported by several investigators. As the results, many reports revealed that 1.7 to 8% of gastric carcinomas and about 3% of intestinal carcinomas contained argentaffin cells, and 13 to 20% of gastric carcinomas and 12 to 15% of intestinal carcinomas contained argyrophil cells. The present study is aimed to evaluate the frequency of argentaffin and argyrophil cells in gastric and intestinal carcinoma in relation to the histopathological patterns. The material consisted of 282 cases of gastric adenocarcinoma and 83 cases of intestinal carcinoma which were diagnosed at the Department of Pathology, Yonsei University College of Medicine from January 1982 through December 1983. All cases were stained with hematoxylin-eosin for histological classification and with silver impregnation techniques for argentaffin and argyrophil granules. Staining for mucin was also performed using PAS stain. The results obtained were as follows: 1) Among 282 cases of gastric carcinomas, Grimelius positive argyrophil cells are noted in 43 cases (15.2%) and Fontana-Masson positive argentaffin cells are noted in 5 cases (1.8%). 2) Among 83 cases of intestinal carcinomas, Grimelius positive argyrophil cells are noted in 15 cases (18.2%) and Fontana-Masson positive argentaffin cells are noted in 1 cases (1.2%). 3) In gastric carcinoma, argyrophil cells are present in 31 cases (17.5%) among 176 male patients and and in 12 cases (11.3%) among 106 female patients. 4) In intestinal carcinoma, argyrophil cells are present in 9 cases (20.4%) amogn 44 male patients, and in 6 cases (15.4%) among 39 female patients. 5) According to classification by Willis, argyrophil cells are present in 12 cases (11.1%) among 108 well to moderately well differentiated adenocarcinoma, 14 cases (14.4%) among 99 poorly differentiated adenocarcinoma, 6 cases (30%) among 20 mucinous carcinoma, and 11 cases (21.1%) among 52 undifferentiated carcinoma. There is no significant difference in the frequency of argyrophil cells in relation to the histological type. 6) According to general classification of intestinal carcinoma, argyrophil cells are present in 9 cases (21.4%) among 42 well to moderately well differentiated adenocarcinoma, 3 cases (15%) among 25 poorly differentiated adrcinom adenocarcinoma and and cases (18.7%) among 16 mucinous carcinoma. The argentaffin cells were noted only in one case, which was the case of well to moderately well differentiated adenocarcinoma. In summary, the results of this study indicates that the frequency of enterochromaffin cells was 15.2% in stomach carcinoma and 18.2% in intestinal carcinoma. According to histological type, the Grimelius positivity was higher in mucinous and undifferentiated type, and higher frequency was noted in male than female.
Female ; Male ; Humans ; Adenocarcinoma ; Stomach Neoplasms

Acute anterior uveitis was induced by footpad injection of bacterial lipopolysaccharide(LPS) in the Lewis rats. To evaluate the distribution and density of MHC class II positive cell, macrophages, B lymphocytes infiltration in the iris and ciliary body 24 hours after injection of bacterial LPS, immunohistochemical study was performed in the wholemount tissues with monoclonal antibodies. Quantitative analysis reveals that the density of MHC class II positive dendritic cells in the iris was 348.5+/-55cells/mm2 in the control group and 448.0+/-176cells/mm2 in the LPS injected group(p<0.05). The density of ED2 positive resident tissue macrophages was higher in the LPS injected group(761.9+/-82cells/mm2) than the control group(620.8+/-57cells/mm2)(p<0.05). ED1 positive macrophages infiltrated significantly in the LPS results in increased group(3225.0+/-522cells/mm2) than in the control group(590.5+/-52cells/mm2).OX33 positive cells were not observed in both control and LPS injected eyes. In conclusion, destruction of blood ocular barrier by infection of LPS results in increased infiltration of OX6 positive cells and macrophages, particularly massive influx of blood monocytes into the iris and ciliary body in acute phase of inflammation. This study confirmed that dendritic cells and macrophages play important roles in acute phase of endotoxin induced uveitis.
Animals ; Antibodies, Monoclonal ; B-Lymphocytes ; Ciliary Body* ; Dendritic Cells ; Immunohistochemistry ; Inflammation ; Iris* ; Macrophages* ; Monocytes ; Rats ; Uveitis* ; Uveitis, Anterior

During the previous several decades the osseointegrated implants have been considered as the most ideal therapy for the fully edentulous or partially edentulous patients. Although the research on the biocompatibility of osseointegration, the oral tactile function, and the histo-neurologic study had been performed, the change of the oral tactile sensibility and maximal occlusal force according to the postinsertion time of implant prostheses has not been studied. The purpose of this study was to compare the oral tactile sensibility and maximal occlusal force of implant prostheses to natural teeth according to the specific postinsertion time intervals. The fifty seven patients treated with Br adegreenemark implants during the recent seven years were involved in this study. The oral tactile sensibility and maximal occlusal force were measured according to the postinsertion time (o6 months, o12 months, o18 months o24 months, and o25 months) 1.The passive tactile threshold of implants was higher than 6-7 times when comparing to natural teeth(P<0.05). 2.The absolute pressure threshold in horizontal and vertical direction for the passive tactile sensibility had decreased significantly in the post insertion time 13-18 months group and had increased significantly in the post insetion time over 25 months group (P<0.05). 3.50% limit thickness and 100% absolute thickness for the active tactile sensibility had decreased significantly in the postinsertion time 7-12 months group (AT50 : P<0.005, AT100: P<0.05) 4.The maximal occlusal force of the implants prostheses had decreased when comparing with natural teeth in the postinsertion time 1-6 months group(P<0.05) and after that there was no significant difference between the implant prostheses and natural teeth(P<0.05). 5.Maximal occlusal force according to the postinsertion time had increased significantly in the 13-18 months group (P<0.05),after that there was no significant difference. 6.There was positive correlation between age and oral tactile sensibility tested in horizontal and vertical direction (PH:r=0.39, PV:r=0.41) and there was no correlation between age and maximal occlusal force, age and active tactile sensibility 7.According to the results or the questionnaire, 76.6% of the patients were satisfied with the function and 96.7% of the patients were satisfied with the sensibility. In conclusion oral tactile sensibility and maximal occlusal force of implants have changed with the postinsertion time. Oral tactile function of implants was lower than that of the natural teeth, but for the maximal occlusal force there was no difference between implants and natural teeth.
Bite Force* ; Humans ; Osseointegration ; Prostheses and Implants ; Surveys and Questionnaires ; Tooth*

Familial adenomatous polyposis (FAP) is a rare hereditary disease that undergo malignant change and recent reports have described a very high incidence (87.5%-100.0%) of congenital hypertrophy of the retinal pigment epithelium (CHRPE) in Gardner's syndrome and familial adenomatous polyposis. Recognization of CHRPE lesion in ophthalmological screening test for the familial members with FAP is helpful not only in prediciting the presence of adenomatous polyposis and malignant carcinoma but also in genetic counselling. Authors have examined and report 2 female patients with similar CHRPE lesions in both fundus and thus diagnosed FAP following surgical examination.
Adenomatous Polyposis Coli* ; Female ; Gardner Syndrome ; Genetic Diseases, Inborn ; Humans ; Hypertrophy* ; Incidence ; Mass Screening ; Retinal Pigment Epithelium* ; Retinaldehyde*

OBJECT: This study was carried out to investigate the effect of pentoxifylline on in vitro fertillization and developmen of preimplantation stage of mouse embryos. MATERIAL AND METHODS:F1 hybrid mice was superovulated with PMSG/hCG and mouse oocytes were recruited. After the normal sperms were incubated with PTX before in vitro fertilization, it was observed whether the fertilization and embryo development was affected or not by the sperm preparation(washing, dilution and no washing or no dilution). And after 1-cell and 2-cell stage of mouse embryos were incubated with PTX, the development to hatching blastocyst was also observed. RESULTS: When in vitro fertilization was revealed by using the washed normal sperms after 0, 3.6 and 7.2 mM PTX incubation, the fertilization rates were 92.5%, 48.8%, 36.8%, respectively. So 3.6 and 7.2 mM groups presented significantly low fertilizatin rate, but the development rates were 93.9%, 85.0%, 95.2%, respectively. Therefore, there were no significant difference between each group. When in vitro fertilization was revealed by using the diluted normal sperms after 0, 3.6, and 7.2 mM PTX incubation, the fertilization rates were 58.6%, 5.4%, 9.4%, respectively. So 3.6 and 7.2 mM groups presented significantly low fertilization rate. The developmental rates were 88.2%, 100%, 100%. And there were no significant difference between each group. When in vitro fertilization was revealed by using the not washed and not diluted normal sperms after 0, 3.6 and 7.2 mM PTX incubation, the fertilizatin rates were 61.2%, 5.7%, 3.8%, respectively. 3.6 and 7.2 mM group presented significantly low fertilization rate. The development rates were 73.3%, 0%, 0%, respectively. So 3.6, 7.2 mM group presented significantly low developmental rate. After 1-cell stage of mouse embryos were incubated in 0, 5, 10, 50 nM of PTX, the development rates were not significantly different among them. After 2-cell stage of mouse embryos were incubated in 0, 5, 10, 50 nM of PTX, the development rates were not significantly different among them. CONCLUSION: In conclusion, when PTX is used in in vitro fertilization program with normal sperms, it may affect the fertilization and embryo development in high concentration. And if PTX concentration is very low, the developmental rate would not be affected. So PTX must not be used to normal sperms and where use of PTX is indicated, it is recommended that remainder PTX must be removed as completely as possible.
Animals ; Blastocyst ; Embryonic Development ; Embryonic Structures ; Female ; Fertilization ; Fertilization in Vitro* ; Mice* ; Oocytes ; Pentoxifylline* ; Pregnancy ; Spermatozoa*

No abstract available.
Animals ; Embryonic Structures* ; Mice*

1,072 cases of ocular injuries, including 271 cases of in-patients, who visited department of ophthalmology of Chungnam National University Hospital from January 1,1981 to December 31, 1985, were clinically analyzed. The results were as follows: 1. The incidence of ocular injuries was 8.1% of all eye patients and 16.0% of all patients admitted to this ophthalmologic department. 2. The incidence was more common in male(82.0%) and in the age of 3rd to 4th decades(53.7%). 3. Monocular injuries accounted for 90.4% of ocular injuries. There was no significant difference in the incidence between the right and left eye. 4. Tho ocular injuries were more common in the spring(27.4%), but in children the incidence was higher during the vacation. 5. The patients who visited this hospital within 24 hours after injury accounted for 89.8%. 6. The most common cause of ocular injuries was fist or finger(15.9%), followed by iron products(12.6%) and traffic accident(10.9%), but the injuries by iron products(32.1%) were most common in the admitted patients. 7. The most common ocular injury was eyelid laceration (15.3%), followed by subconjunctival hemorrhage(12.5%) and conjunctival foreign body(8.6%). In the cases of in-patients, corneal laceration(21.2%) was most common, followed by corneoscleral laceration(14.9%) and lens perforation(10.2%). The corneal perforation was 52.1% of all perforating eye injuries. 8. Surgical procedure included corneal suture(21.1%), lensectomy(12.5%), enucleation or evisceration(11.2%), and others. 9. Visual acuity was improved in most cases by treatment, but the corrected vision after treatment was less than 0.1 in 33.2%, which was mainly due to the perforating eye injuries. 10. The most common complication of ocular injuries after treatment was corneal opacity(36.3%), followed by secondary glaucoma(14.6%) and traumatic cataract(6.4%).
Child ; Chungcheongnam-do ; Corneal Perforation ; Eye Injuries ; Eyelids ; Humans ; Incidence ; Iron ; Lacerations ; Ophthalmology ; Visual Acuity

Experimental total retinectomy was performed in pigmented rabbit eyes to in vestigate its long-term effects on the operated and nonoperated opposite eyes from both clinical and histological aspects. There was no significant change in intraocular pressure between the preoperative and postoperative measurements during the six months after surgery. Rubeosis iridis, intraocular hemorrhage and phthisis bulbi were not evident in the operated and opposite eyes. Light microscopic examination revealed congestion of the choroidial vessels. There were scattered areas of retinal pigment epithelial cell proliferation. Transmission electron microscopic examination showed apiral mounding of the retinal pigment epithelium. Apical processes were short and reduced in number. Apical processes of the retinal pigment epithelial cells interdigitated with surface processes of the proliferating retinal pigment epithelial cells. There were numerous melanosomes in the cytoplasm of the proliferating retinal pigment epithelial cells. Cell junctions between the retinal pigment epithelial cells were well preserved.
Animals ; Intraocular Pressure ; Longitudinal Studies ; Pigment Epithelium of Eye/ultrastructure ; Prognosis ; Rabbits ; Retina/*surgery

To identify the MHC class II expressing cells and macrophages in the iris, immunohistochemistry was carried out using monoclonal antibodies specific to MHC class II antigens(OX6), resident tissue macrophages(ED2) and monocyte-macrophage lineage(ED1) on wholemounts of iris from normal Lewis rats. A number of OX6 positive cells are distributed evenly in the iris. Morphology of OX6 positive cells are dendritiform, round, or pleomorphic. OX6-positive and dendritiform cell are presumed to be dendritic cells. ED2 positive cells are more closely arranged than OX6 positive cells. Most of ED2 positive cells are dendritiform. Most of ED1 positive cells are round. These cells might have important roles in the process of immune mechanism to various antigens in the anterior segment of the eye.
Animals ; Antibodies, Monoclonal ; Dendritic Cells ; Immunohistochemistry ; Iris* ; Macrophages* ; Rats*

The post-cataract astigmatism was evaluated clinically between the groups with loose and tight suture in 47 patients(48 eyes) who had taken intraocular lens implantation with extracapsular cataract extraction. The results were as follows; 1. The post-cataract astigmatism in the tight suture group was significantly larger than the loose suture group in the early postoperative period. 2. There was no significant difference of the degree of astigmatism between the groups with loose and tight suture at postoperative 8th week. 3. Postoperative astigmatism was smaller than preoperative astigmatism in both groups at postoperative 8th week. 4. Decreasing velocity of with the rule astigmatism after operation is similar in both groups. Changing time of astigmatism axis: Tight suture group; 6th-8th week. Loose suture group; 3rd-4th week.
Astigmatism* ; Axis, Cervical Vertebra ; Cataract Extraction ; Lens Implantation, Intraocular ; Postoperative Period ; Sutures*