1.Immunolocalization of the 150 kDa protein in cyst fluid of Taenia solium metacestodes.
Hyun Jong YANG ; Young Bae CHUNG
The Korean Journal of Parasitology 2004;42(2):81-84
The 150 kDa protein of cyst fluid (CF) of Taenia solium metacestodes was purified by ammonium sulfate fractionation and Superose 6 HR gel filtration chromatography. The purified protein consisted of three subunits (15, 10 and 7 kDa proteins), which were analyzed with the use of a 7.5-15% gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunofluorescence study was carried out by using immunize specific polyclonal antibody. Positive reactions were noticed at bladder walls, calcareous corpuscles, granules of cyst fluid and some host tissue surrounding the bladder wall of the metacestodes. These results suggest that the 150 kDa protein was secreted into host tissues, inducing immune responses in the host, and it may play important roles in the cellular physiology of the parasites.
Animals
;
Chemical Fractionation
;
Chromatography, Gel
;
Cyst Fluid/*chemistry/metabolism/parasitology
;
Cysticercosis/*metabolism/parasitology
;
Electrophoresis, Polyacrylamide Gel
;
Helminth Proteins/chemistry/*isolation & purification
;
Mice
;
Mice, Inbred BALB C
;
Microscopy, Fluorescence
;
Molecular Weight
;
Support, Non-U.S. Gov't
;
Swine
;
Swine Diseases/parasitology
;
Taenia solium/*metabolism
2.Prefrontal Cortex and Schizophrenia.
Young Chul CHUNG ; Hong Bae EUN
Journal of the Korean Society of Biological Psychiatry 1998;5(2):184-196
With a rapid development of neuroscience, the theories related to the pathophysiology of schizophrenia have been changed a lot from a simple hyperdopaminergic one to the various complicated ones. Among these, the theories regarding prefrontal cortex(PFC) pathology as a cause of schizophrenia are gaining more recognition as the results of neuroimaging and neuropsychological tests in schizophrenia consistently report abnormalities in PFC. Therefore, we first reviewed the unique characteristics of PFC in anatomy, neurochemistry and neurophysiology to enhance an understanding of those ones. Secondly, various neurotransmitter, neurodevelopmental and neural network theories of schizophrenia introduced recently were reviewed in terms of PFC pathology.
Dopamine
;
Neurochemistry
;
Neuroimaging
;
Neurophysiology
;
Neuropsychological Tests
;
Neurosciences
;
Neurotransmitter Agents
;
Pathology
;
Prefrontal Cortex*
;
Schizophrenia*
3.Insall-Burstein Posterior Stabilized Knee Prosthesis: Preliminary Report
Dae Kyung BAE ; Young Kwon KIM ; Sang Wook BAE ; Young Ryong KIM ; Chung O KIM
The Journal of the Korean Orthopaedic Association 1983;18(6):1148-1154
No abstract available in English.
Knee Prosthesis
;
Knee
4.Clinical and microbiological study of ureaplasma urealyticum.
Bae Geun YOAN ; Young Cheon JANG ; Jae Hoon CHUNG
Korean Journal of Obstetrics and Gynecology 1993;36(7):2270-2278
No abstract available.
Ureaplasma urealyticum*
;
Ureaplasma*
5.Enzymatic N-glycan analysis of 31 kDa molecule in plerocercoid of Spirometra mansoni (sparganum) and its antigenicity after chemical oxidation.
Young Bae CHUNG ; Yoon KONG ; Hyun Jong YANG
The Korean Journal of Parasitology 2004;42(2):57-60
A highly specific antigenic protein of 31 kDa from plerocercoid of Spirometra mansoni (sparganum) was obtained by gelatin affinity and Mono Q anion-exchange column chromatography. The purified 31 kDa protein was subjected to N-glycan enzymatic digestion for structural analysis. The relative electrophoretic mobility was analyzed by SDS-PAGE, before and after digestion. On SDS-PAGE after enzymatic digestion, the 31 kDa protein showed a molecular shift of approximately 2 kDa, which indicated the possession of complex N-linked oligosaccharides (N-glycosidase F sensitive) but not of high-mannose oligosaccharides (endo-beta-N-acetylglucosaminidase H, non-sensitive). Chemically periodated 31 kDa protein showed statistically non-significant changes with human sparganosis sera by enzyme linked immunosorbent assay (ELISA). Therefore, the dominant epitopes of the 31 kDa molecule in human sparganosis were found to be mainly polypeptide, while N-glycans of the antigenic molecule in sparganum was minimal in anti-carbohydrate antibody production.
Animals
;
Antigens, Helminth/*analysis/chemistry/immunology/metabolism
;
Carbohydrates/analysis/immunology
;
Chromatography, Affinity
;
Chromatography, Ion Exchange
;
Electrophoresis, Polyacrylamide Gel
;
Enzyme-Linked Immunosorbent Assay
;
Epitopes/analysis/immunology
;
Glucosaminidase/metabolism
;
Human
;
Peptide-N4- (N-acetyl-beta-glucosaminyl) Asparagine Amidase/metabolism
;
Periodic Acid/chemistry
;
Sparganosis/*parasitology
;
Sparganum/immunology/metabolism
;
Spirometra/immunology/*metabolism
;
Support, Non-U.S. Gov't
6.A subclassification of conduct disorder by child behavior checklist.
Hwan Bae LEE ; Young Sook KWACK ; Ki Chung PAIK
Journal of Korean Neuropsychiatric Association 1992;31(2):351-362
No abstract available.
Checklist*
;
Child
;
Child Behavior*
;
Child*
;
Conduct Disorder*
;
Humans
7.Degranulation of human eosinophils induced by Paragonimus westermani-secreted protease.
Myeong Heon SHIN ; Young Bae CHUNG ; Hirohito KITA
The Korean Journal of Parasitology 2005;43(1):33-37
Eosinophil degranulation is considered to be a key effector function for the killing of helminthic worms and tissue inflammation at worm-infected lesion sites. However, relatively little data are available with regard to eosinophil response after stimulation with worm-secreted products which contain a large quantity of cysteine proteases. In this study, we attempted to determine whether the degranulation of human eosinophils could be induced by the direct stimulation of the excretory-secretory products (ESP) of Paragonimus westermani, which causes pulmonary paragonimiasis in human beings. Incubation of eosinophils for 3 hr with Paragonimus-secreted products resulted in marked degranulation, as evidenced by the release of eosinophil-derived neurotoxin (EDN) in the culture supernatants. Moreover, superoxide anion was produced by eosinophils after stimulation of the ESP. The ESP-induced EDN release was found to be significantly inhibited when the ESP was pretreated with protease inhibitor cocktail or the cysteine protease inhibitor, E-64. These findings suggest that human eosinophils become degranulated in response to P. westermani-secreted proteases, which may contribute to in vivo tissue inflammation around the worms.
Animals
;
*Cell Degranulation
;
Cysteine Endopeptidases/metabolism/*physiology
;
Eosinophil-Derived Neurotoxin/metabolism
;
Eosinophils/*physiology
;
Humans
;
Paragonimus westermani/*enzymology
;
Research Support, Non-U.S. Gov't
;
Superoxides/metabolism
;
Time Factors
8.Is there evidence that Kudoa septempunctata can cause an outbreak of acute food poisoning?
Young Bae CHUNG ; Jong Myon BAE
Epidemiology and Health 2017;39(1):2017004-
After publishing results of a study that revealed diarrheagenic and emetic activity in 4-5-day old mice infected with Kudoa septempunctata (K. septempunctata) spores, the Korea Centers for Disease Control and Prevention reported 11 events of “Kudoa food poisoning” in 2015. The epidemiological design of the previous study was descriptive rather than analytical; therefore, this study aimed to further investigate the pathogenicity of K. septempunctata. Academic articles showing evidence of the pathogenicity of K. septempunctata were searched via PubMed using the citation discovery tool. Information regarding the kinds of experimental animals and inoculum spores used, as well as study results were extracted. Four articles evaluating the pathogenicity of Myxospran parasites were selected; the first article suggested the pathogenicity of K. septempunctata, while the remaining three articles reported no abnormal symptoms or histopathologic changes. Our findings indicate that there is weak evidence supporting the pathogenicity of K. septempunctata. Further studies evaluating the pathogenicity of K. septempunctata are needed urgently.
Animals
;
Centers for Disease Control and Prevention (U.S.)
;
Food Parasitology
;
Foodborne Diseases
;
Intestinal Diseases, Parasitic
;
Korea
;
Mice
;
Myxozoa
;
Parasites
;
Spores
;
Virulence
9.Is there evidence that Kudoa septempunctata can cause an outbreak of acute food poisoning?.
Young Bae CHUNG ; Jong Myon BAE
Epidemiology and Health 2017;39(1):e2017004-
After publishing results of a study that revealed diarrheagenic and emetic activity in 4-5-day old mice infected with Kudoa septempunctata (K. septempunctata) spores, the Korea Centers for Disease Control and Prevention reported 11 events of “Kudoa food poisoning” in 2015. The epidemiological design of the previous study was descriptive rather than analytical; therefore, this study aimed to further investigate the pathogenicity of K. septempunctata. Academic articles showing evidence of the pathogenicity of K. septempunctata were searched via PubMed using the citation discovery tool. Information regarding the kinds of experimental animals and inoculum spores used, as well as study results were extracted. Four articles evaluating the pathogenicity of Myxospran parasites were selected; the first article suggested the pathogenicity of K. septempunctata, while the remaining three articles reported no abnormal symptoms or histopathologic changes. Our findings indicate that there is weak evidence supporting the pathogenicity of K. septempunctata. Further studies evaluating the pathogenicity of K. septempunctata are needed urgently.
Animals
;
Centers for Disease Control and Prevention (U.S.)
;
Food Parasitology
;
Foodborne Diseases*
;
Intestinal Diseases, Parasitic
;
Korea
;
Mice
;
Myxozoa
;
Parasites
;
Spores
;
Virulence
10.A case of multiple congenital anomaly.
Chang Qyun CHUNG ; Hyun Young BAE ; Deok Rye KIM ; Yong Hae PARK ; Ho Soon CHUNG
Korean Journal of Obstetrics and Gynecology 1992;35(9):1407-1413
No abstract available.