No abstract available.
Lupus Coagulation Inhibitor*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
DNA* ; Flow Cytometry*

No abstract available.
Lymphocytes*

BACKGROUND: The recently developed nucleic acid amplification methods may provide us with very sensitive, specific and rapid tests for the detection of M. tuberculosis. So the aim of this study was to compare the commercial Amplicor M. tuberculosis kit and our in-house polymerase chain reaction (PCR) with the conventional culture and direct AFB staining method. Materials and METHODS: Among the total of 2,340 clinical specimens, 1,314 sputum samples were tested for the presence of M. tuberculosis by Amplicor PCR and 1,026 sputum samples were tested by in-house PCR performing with resin matrix preparation and DNA extraction, synthesized primer pair, detection using agarose gel electrophoresis. RESULTS: One hundred-seventeen specimens were positive by Amplicor PCR, 105 were positive by in-house PCR, 185 were positive by culture. The sensitivity of the Amplicor PCR for all of the specimens and for smear-positive and smear-negative specimens was 92.9%, 97.9% and 88.2%, respectively after discrepant analysis. The sensitivity of the in-house PCR for all of the specimens and for smear-positive and smear-negative specimens was 80.0%, 93.6% and 65.5%, respectively after discrepant analysis. The specificity of the Amplicor PCR and in-house PCR for all of the specimens was 97.9% and 99.0%, respectively. CONCLUSIONS: Amplicor PCR was more sensitive than in-house PCR, but there was another problems such as high false positive rate and high cost. So PCR may certainly become very useful in microbiological laboratories if PCR method is selected according to the laboratory conditions.
DNA ; Electrophoresis, Agar Gel ; Mycobacterium tuberculosis* ; Mycobacterium* ; Polymerase Chain Reaction* ; Sensitivity and Specificity ; Sputum* ; Tuberculosis

No abstract available.
Precursor Cell Lymphoblastic Leukemia-Lymphoma*

We report a case of weak subgroup A in a patient diagnosed as myelodysplastic syndrome. The patient's red cells was typed as O and his serum had anti-B. Red blood cell antibody screening test was negative. Am was confirmed by adsorption-elution test and saliva study.
Erythrocytes ; Humans ; Mass Screening ; Myelodysplastic Syndromes ; Saliva

No abstract available.
Animals ; Horns* ; Nevus, Sebaceous of Jadassohn*

The purpose of this study is to estimate the nutrient intakes and food habits among preschool children in Kyungjoo city. The subjects were 210 preschool children, aged 4-6 years. Measurements of the weight, height, chest circumference, and head circumference of the children were conducted. And general home environment and factors related to eating habits for preschool children were collected using a questionnaire that included information about family income, parents' education and occupations. The average weight-length index (WLI) for the subjects was 103.9%. Using the WLI, 20.0% of the preschool children were underweight, 48.6% were normal, 19.0% were overweight, and 12.3% were obese. On the Rohrer index, 13.8% of the preschool children were underweight, 38.5% were normal, and 47.7% were over weight or obese. The average daily intake (% of RDA) of energy and each nutrient was 1323.5 kcal (81.3%), Ca 484.3 mg (80.7%), Fe 7.05 mg (88.1%), vitamin A 420.0 RE (105%), vitamin B1 0.76 mg (95.0%), vitamin B2 0.87 mg (87.0%), and vitamin C 53.1 mg (106.2%), respectively. In particular, older subjects had lower intake in RDA % of calcium and iron. The energy intake ratio from snacks was much higher than the recommended level of the preschool children. With regard to frequency of regularity of breakfast, 1.9% of preschool children skipped every morning and 7.6% of the children skipped more than 5 per week. With regard to the intake frequency of vegetables, fruits, complex carbohydrates, and milk, 13.3%, 19.9%, 22.8%, and 41.8% of the children ate more than 5 times per week. The eating habit score was positively correlated (r = 0.18, p < 0.05) with household income. This study suggests that nutrition education to increase fruit and vegetable consumption for preschool children should be emphasized. Also a nutrition education program is needed to enhance consuming calcium and iron intake for adequate growth.
Ascorbic Acid ; Breakfast ; Calcium ; Carbohydrates ; Child ; Child, Preschool* ; Eating* ; Education ; Energy Intake ; Family Characteristics ; Food Habits ; Fruit ; Gyeongsangbuk-do* ; Head ; Humans ; Iron ; Milk ; Occupations ; Overweight ; Riboflavin ; Snacks ; Thiamine ; Thinness ; Thorax ; Vegetables ; Vitamin A ; Surveys and Questionnaires

BACKGROUND: Multidrug resistant tuberculosis (MDRTB) strains rely on the prompt availability of drug susceptibility test results. We evaluated the reliability and turnaround time of MGIT 960 system, automated version of the MGIT, for antimicrobial susceptibility test of Mycobacteria tuberculosis. METHODS: Ninety six isolates have been tested for susceptibility to isoniazid (INH), rifampin (RIF), ethambutol (EMB) and streptomycin (SM). Results were compared with those obtained by traditional solid media (absolute concentration method, indirect method). RESULTS: There was no statistically significant difference between the susceptibility testing results of the two methods except for EMB. Discrepant results were obtained for 8 isolates (8.3%) with INH, for 3 isolates (3.1%) with RIF, for 13 isolates (13.5%) with EMB, for 10 isolates (10.4%) with SM. Using the indirect method as the gold standard, the sensitivity of INH, RIF, EMB and SM susceptibility testing by the MGIT system were 94.1%, 98.8%, 86.7% and 90.0%, respectively. The specificity were 85.7%, for INH and RIF and 83.3%, for EMB and SM. Turnaround times were significant shorter in MGIT (average 12 days) than in solid media (average 57 days) (P < 0.05) CONCLUSIONS: These data demonstrate that the MGIT system is accurate and rapid for INH, RIF and SM susceptibility test of M. tuberculosis, but EMB susceptibility testing requires further evaluation.
Ethambutol ; Isoniazid ; Mycobacterium tuberculosis* ; Mycobacterium* ; Rifampin ; Sensitivity and Specificity ; Streptomycin ; Tuberculosis