Cell Transformation, Neoplastic ; metabolism ; Humans ; Liver Neoplasms ; metabolism ; Non-alcoholic Fatty Liver Disease ; metabolism ; PPAR alpha ; metabolism

Animals ; Fructose ; Humans ; Non-alcoholic Fatty Liver Disease ; Risk Factors

Objective To observe the effect of several residual adhesive methods on the enamel surface ,and conduct lab evalua-tion .Methods Sixty premolars extracted because of orthodontic treatment .And all the teeth were randomly divided into 3 groups . Group 1:tungsten carbide burs + silicon particles ;Group 2 :ultrasonic scaling + silicon particles ;Group 3:silicon particles ,each with 20 premolars .After underwent several methods ,the surface roughness differences ,operation time were determined and ob-served with the scanning electron microscope .And the result was statistically analyzed .Results There were significant differences in the surface roughness and operation time among the three groups (P<0 .05) ,The scanning electron microscope after polishing showed that the teeth surface had different degrees of injury ,the silica particles group had less superficial scratch .Conclusion The tungsten carbide burs and ultrasonic instrument for debonding before the silica particles had less superficial scratch .

This article reviews the immunopathogenesis of cystic echinococcosis in the following seven aspects: innate immunity,establishment phase immunity,cystic phase immunity,influencing factor of CD4+ T cell polarization,cytokine function in infected host,Echinococcus granulosus infection and allergy,and immune evasion mechanism.

Objective:To investigate apoptosis of spleen cells in infected mice by immunization with recombinant BCG-Eg95 vaccine of Echinococcus granulosus(Eg) and against challenge with Eg protoscoleces.Methods:BALB/c mice were vaccinated with the vaccine subcutaneously,intranasally,orally and intramuscularly respectively.The mice were then challenged with Eg protoscolexes at 8w of vaccination and sacrificed in 18w of infection to get spleen.Spleen cells were separated to measure apoptotic rate by FACsort with BCG and PBS served as control.Results:Apoptotic rate in the immunization group was lower than that in the control.Apoptotic rates in the oral or intramuscular group were significantly lower than that in the subcutaneous or intranasal group.Conclusion:Apoptosis of spleen cells in mice may be induced by infection with hydatid cyst,but is inhibited by immunization with rBCG-Eg95 vaccine.Oral or intramuscular vaccination may be the good regimen.

Objective To study the effect of calcium on human peritoneal mesothelial cells (HPMCs). Methods Proliferation abilities of HPMCs were assessed by tetrazolium salt colorimetry assay (MTT assay) and the levels of LDH in the supernatant were detected in all groups to evaluate the damage of HPMCs. The expression of TNF-α in cytoplasm was detected by immunohistochemistry. Results Calcium enhanced proliferation of HPMCs in a time-dependent manor(P＜0.01), especially calcium with 1.25 mmol/L(0.5098±0.016,0.6763±0.048) and 1.0 mmol/L(0.4853±0.016,0.6678±0.076). Calcium with different concentrations significantly increased the levels of LDH in HPMCs in a time-dependent manor, while the effect of calcium with 1.25 mmol/L was lowest(17.78±1.18,23.60±1.39,P＜0.01). Calcium with 2.0 mmol/L[(42.61±3.29)%] and 1.75 mmol/L[(33.32±1.88)%] significantly up-regulated the expression of TNF-α(P＜0.01) . Conclusions High calcium damaged HPMCs and up-regulated the expression of TNF-αby HPMCs, while physical calcium (1.25 mmol/L)can protect peritoneum and prevent peritoneal fibrosis.

Objective To explore feasibility of laparoscopic hepatectomy for hepatic hemangioma.Methods Twelve patients were treated by laparoscopic hepatectomy, including left lateral lobectomy in 5 cases and local liver resection in 7 cases. Three cases of hepatic hemangioma associated with gallbladder stone were performed cholecystectomy synchronously. Results Laparoscopic procedures were successfully performed in all 12 cases. The mean operative time was 155 min. The mean blood loss was 230 mL. The mean postoperative hospital stay was 8 days. The pospostoperative recovery was smooth except that 1 case had pulmonary infection. During a follow-up of 6-20 months for 12 cases,there were no recurrence. Conclusion Laparoscopic hepatectomy for hepatic hemangioma is safe and feasibile with good effect under the condition of strict indication selection and experienced surgeons operating.

Objective To investigate the protection by immunization with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine of Echinococcus multilocularis(Em) and against challenge of Em protoscolexes.Methods BALB/C mice were subcutaneously or intranasally vaccinated with mixed recombinant BCG vaccines respectively.Eight weeks later,the immunized mice were challenged with Em protoscolexes.On the 18th week after vaccination,the mice were killed to count the rate of reduced alveolar echinococcus weight and to examine the levels of IgG and its subclasses,IgE;the spleen cells were separated to measure subsets of CD4+ and CD8+ T cells by FACsort.Blank vector,BCG and PBS served as control.Results In the immunized groups,the rate of reduced alveolar echinococcus weight was 45.29%-76.47%;the levels of IgG,IgG2a,IgG2b and IgE increased apparently while IgG1 and IgG3 decreased remarkably;CD4+ subset and the ratio of CD4+/CD8+ increased obviously,but CD8+ subset had no change.Conclusion Intranasal vaccination with mix recombinant BCG-EmⅡ/3 and BCG-Em14-3-3 vaccine is a good way for immunization.IgG,IgG2a,IgG2b,IgE and CD4+ T cell subset may play an important role in protecting against challenge of Em protoscolexes.

Objective:To investigate the influence of pCD-Em10 on immune responses in mice infected with alveolar hydatid cyst of Echinococcus multilocularis(Em).Methods:BALB/c mice were intramuscularly vaccinated with pCD-Em10 and then challenged with Em protoscoleces at the 8 w after vaccination.The mice were killed in 18th week of infection to count the rate of reduced alveolar hydatid cyst weight;The spleens were gathered to separate splenocytes,and the subsets of CD4+and CD8+T cells were measured by FACsort.The splenocytes were cultured by stimulation with EmAg or ConA,their supernatants were gathered to measure level of IL-2,IFN-?,TNF-? and IL-4 by ELISA Kits.Apoptotic rate of splenocytes was measured by FACsort.BCG or PBS injection served as control.Results:In the group of PCD-Em10 immunization,the rate of reduced alveolar hydatid cyst weight was 71.17%,CD4+ subset increased obviously,and the levels of IFN-? and TNF-? increased remarkably,but that of IL-4 decreased.Apoptotic rate in the pCD-Em10 group was lower than that in the PBS control.Conclusion:pCD-Em10 may induce a protective immune response in mice infected with alveolar hydatid cyst of Echinococcus multilocularis.

Objective To construct recombinant Bb-Em14-3-3 vaccine of Echinococcus multilocularis and to investigate expression efficiency of Em14-3-3 antigen encoding gene in Escherichia coli BL21(DE3).Methods Em14-3-3 antigen gene was amplified by PCR.Then the gene was cloned into Escherichia coli-Bifidobacteria shuttle plasmid pGEX-1? T containing gluathione-S-transferaze(GST) gene to construct pGEX-Em14-3-3.The recombinant plasmid was electroporated into Bifidobacteria bifidum(Bb) to construct rBb-Em14-3-3 vaccine.The vaccine was identified with PCR and restriction-endonuclease digestion.The expression of pGEX-Em14-3-3 was induced with isopropyl-?-D-thiogalactopyranosid(IPTG) and fusion protein Em14-3-3/GST was examined by SDS-PAGE and Western blot techniques.Results 530 bp gene of Em14-3-3 was successfully amplified by PCR and cloned into pGEX-1? T by restriction analysis.rBb-Em14-3-3 vaccine was successfully constructed by PCR and restriction-endonuclease digestion.It was demonstrated with SDS-PAGE and Western blot that the fusion protein Em14-3-3/GST was expressed in E.coli,BL21.The expression efficiency is 23%.Conclusion rBb-Em14-3-3 vaccine of Echinococcus multilocularis was successfully constructed.The plasmid pGEX-Em14-3-3 was highly expressed in E.coli in fused form with GST and this kind of protein shows specific antigenicity.