Objective To study the detection value of serum alpha -L -fucosidase(AFU)and alpha fetoprotein 3(AFP -L3)in primary hepatocellular carcinoma(HCC).Methods 100 patients with primary HCC were enrolled as HCC group,54 cases without HCC were selected as non HCC group,100 healthy persons were selected as healthy group.Serum AFU and AFP-L3 levels in three groups were determined.The serum AFU was measured by the chemical colorimetry,and the AFP-L3 level was determined by chemiluminescence immunoassay.Results The serum levels of AFU in HCC group,non HCC group and healthy group were (71.61 ±3.01)U/L,(11.06 ±2.15)U/L, (8.54 ±1.28)U/L.The serum levels of AFP-L3 in HCC group,non HCC group and healthy group were (30.14 ± 2.93)%,(3.27 ±0.83)%,(2.77 ±0.62)%.The serum levels of AFU and AFP-L3 in HCC group were signifi-cantly higher than those in non HCC group and healthy group (t=13.172,9.770,16.036,7.160,all P<0.01 ). There were no significant differences in serum AFU and AFP-L3 levels between the non HCC group and the healthy group (t=0.761,0.580,all P>0.05).The positive rates of AFU were 87.00%,9.26%and 3.00%in HCC group, non HCC group and healthy group.The positive rate of AFU in HCC group was significantly higher than that in non HCC group and healthy group (χ2 =34.06,57.62,all P<0.01 ).There was no significant difference in the positive rate of AFU between the non HCC group and the healthy group(χ2 =3.81,P>0.05).The positive rates of AFP-L3 were 89.00%,7.41%and 2.00%in HCC group,non HCC group and healthy group.The positive rate of AFP-L3 in HCC group was significantly higher than that in non HCC group and healthy group (χ2 =41.06,77.62,all P<0.01).There was no significant difference in the positive rate of AFP -L3 between the non HCC group and the healthy group (χ2 =3.14,P>0.05 ).There were no significant differences in serum tumor diameter about AFU, AFP-L3(t=0.167,0.579,0.201,0.601,all P>0.05),and there were significant differences about TNM stage, lymph node metastasis,and tumor thrombus (t =6.156,9.105,10.161,8.120,9.770,12.821,all P<0.05). Conclusion Serum AFU and AFP -L3 can not only help the diagnosis of primary liver cancer,but also can determine the prognosis of patients.

Objective To investigate the dynamic changes of serum high mobility group box-1 (HMGB1)and epithelial neutrophil-activing peptide-78(ENA-78)associated with secondary brain edema in patients following acute brain injury.Methods The serum HMGB1 and ENA-78 in 110 patients with acute brain injury were determined by using enzyme-linked immunosorbent assay(ELISA)12 hours,3 days and the 5 days after acute brain injury.The outcomes were analyzed by t-test and estimated well with clinical symptoms,imaging data and Glasgow Outcome Scale(GOS)in combination of.Results The levels of HMGB1 and ENA-78 increased significantly with lowering the score of GCS 12 hours after acute brain injury.The more severity of acute brain injury resulted in more production of HMGB1 and ENA-78 and longer period of persisted and peaked brain edema(all P ＜0.01).HMGB1 levels had positive correlation with severity and persistence of brain edema(r =0.69,P ＜0.01 and r =0.70,P ＜0.01).ENA-78 levels had positive correlation with severity and persistence of brain edema(r =0.62,P ＜ 0.01 and r =0.65,P ＜ 0.01).Furthermore,there were statistical differences in HMGB1 and ENA-78 levels between different GOS groups.Compared with good outcome group and normal control group,the HMGB1 and ENA-78 levels in poor outcome group persistently increased and were higher within 5 days after brain injury(P ＜ 0.01 or P ＜0.05).There was a correlation between serum HMGB1 and ENA-78 levels in patients with acute brain injuries(r =0.68,P ＜ 0.01).Conclusions The changes of serum HMGB1 and ENA-78 levels were closely associated with secondary brain edema in patients following acute brain injury.

Objective To observe the changes of serum vascular endothelium-cadherin (VE-cadherin) and plasma arginine vasopressin (AVP) in patients with acute brain injury, and to investigate the VE-cadherin and plasma AVP related to brain edema and prognosis following acute brain injury. Method ELISA was used to measure the serum VE-cadherin level and radioimmunoassay was used to measure serial concentration of AVP in plasma of 110 patients with acute brain injury 12 hours and 3 and 5 days after brain injury. The outcome was estimated in combination of clinical manifestation, iconographic findings and Glasgow Outcome Scale (GOS). Results The levels of VE-cadherin and AVP increased significantly over 12 hours after brain injury with reduction of GCS. The severer brain injury resulted in higher levels of VE-cadherin and AVP, and prolongation of peak brain edema (P < 0.01). VE-cadherin levels showed positive correlation with severity of brain edema (r = 0.69, P < 0.01) and prolongation of brain edema (r = 0.70.P < 0.01). AVP levels had positive correlation with severity of brain edema (r = 0.65, P < 0.01) and prolongation of brain edema (r = 0.64, P < 0.01). Furthermore, there were significant increases in VE-cadherin and AVP levels in patients with low GOS groups (P < 0.01). The VE-cadherin and AVP leveb in poor outcome group persistently increased for 5 days after brain injury and were higher than those in good outcome group and normal control group. There were a correlation between VE-cadherin and AVP in patients with acute brain injury (r = 0.75, P < 0.01). Conclusions VE-cadherin and AVP leveb are closely associated with the prognosis of patients after acute brain injury. VE-cadherin and AVP may play pivotal roles in the pathogenesis of brain edema after acute brain injury.

Objective To investigate the possible correlation between obstructive sleep apnea hypopnea syndrome(OSAHS)and serum epithelial neutrophil-activing peptide-78(ENA-78)and VE-cadherin in pregnant patients.Methods One hundred and two pregnant women combined with OSAHS,42 of which were with hypertension,and 36 healthy pregnant women were underwent polysomnography(PSG),and their apnea hypopnea index(AHI)was recorded.Based on the apnea hypopnea index(AHI),they were divided into mild OSAHS group(36 patients),moderate OSAHS group(34 patients)and severe OSAHS group(32 patients)Serum ENA-78 and VE-cadhefin levels were detected by enzyme-linked immunosorbent assay(ELISA),and mean arterial pressure(MAP)was measured simultaneously.Results The values of AHI,MAP,ENA-78 and VE-cadherin in OSAHS with hypertension were significantly higher than those of OSAHS only group(t =13.46,11.65,12.47,13.36,all P ＜ 0.01)and the control group(t =16.72,15.31,16.35,16.73,all P ＜ 0.01).The levels of serum ENA-78 and VE-cadherin were gradually significantly decreased in the order of severe OSAHS group,moderate OSAHS group and mild OSAHS group(P ＜0.01).The AHI of OSAHS group was significantly correlated with MAP,VE-cadherin,ENA-78(r =0.75,0.71,0.68,all P ＜ 0.01).ENA-78 of OSAHS group were positively correlated with MAP and VE-cadherin(r =0.64,0.67,all P ＜ 0.01).Conclusions The condition OSAHS in pregnant patients is closely related to the levels of ENA-78 and VE-cadherin.Pregnancy combined with OSAHS may take part in the pathological process of hypertensive disorder complicating pregnancy.

Objective To investigate the roles of serum visfatin and VE-cadherin in the pathogenesis of psoriasis vulgaris and their clinical significance. Methods Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum levels of visfatin and VE-cadherin in 78 patients with psoriasis vulgaris before and after treatment and in 30 normal human controls. The relationship between the two parameters and disease activity was evaluated. Results The serum levels of visfatin and VE-cadherin were significantly higher in untreated patients with psoriasis than in the normal controls (I = 10.53, 10.16, both P< 0.01), and in patients with active psoriasis than in those with stable psoriasis (t = 12.47, 13.11, both P< 0.01). The treatment caused a marked decrease in the serum levels of visfatin and VE-cadherin in patients. The serum level of visfatin was positively associated with the level of VE-cadherin and PASI scores (r = 0.58, 0.64, P < 0.01) in patients with psoriasis. Conclusion Visfatin and VE-cadherin may be used as parameters for evaluating disease activity of psoriasis vulgaris.

Objective To investigate the changes of high mobility group box-1 ( HMGB1 ) and VE-cadherin in serum of children with viral myocarditis and their clinical significance. Methods The serum levels of HMGB1 and VE-cadherin were detected by ELISA in 52 children with viral myocarditis, and 36 normal healthy children were enrolled as control. CK-MB was also measured in all subjects enrolled into the study. Results The serum levels of HMGB1, VE-cadherin and CK-MB in children with acute stage viral myocarditis (HMGB1 :[5.14 ±0. 23] mg/L;VE-cadherin: [5.36 ±0. 92] mg/L;CK-MB: [31.42 ± 3.22] U/L)were significantly higher than those with recovery stage viral myocarditis ( HMGB1: [ 0. 92 ± 0. 14 ] mg/L, VE-cadherin: [2. 93 ±0. 64] mg/L; CK-MB: [ 13.75 ± 3.18 ] U/L) ( t = 11.37,10. 26 and 12. 17 respectively ,Ps ＜ 0. 01 )and control (HMGB1 :[ 0. 86 ± 0. 12 ] mg/L; VE-cadherin: [ 2. 86 ± 0. 65 ] mg/L; CK-MB: [ 12. 83 ±3.04] U/L) (t = 12.06,11.19 and 12. 64 respectively,Ps ＜0.01 ). However,we found no significant difference in the serum levels of HMGB1, VE-cadherin and CK-MB between recovery stage viral myocarditis group and the control ( t = 1.26,1.19,1.43, Ps ＞ 0. 05 ). There were positive correlations between HMGB1 and VE-cadherin,CK-MB (r = 0. 73,0. 79, Ps ＜ 0. 05 ) ;and positive correlation between VE-cadherin and CK-MB (r= 0. 82, P ＜0.05). Conclusion HMGB1 and VE-cadherin may play roles in the viral myocarditis pathogenesis, which can be new prognosis factors for viral myocarditis.

Objective To investigate the changes and clinical implications of VE-eadherin during the courseof progressive cerebral infarction.Method One hundred sixty-seven patients with acute cerebral infarction of Rong Jun General Hospital of Shandong Province and the Central People' s Hospital of Tengzhou were diagnosed in our hospital from May 2006 to July 2007,were diagnosed according to the ill%gnome criteria set by the 4th national cerebrovascular disease conference in 1995.Of them there were 102 ases with progressive cerebral infarction patients and 65 cases with non-progressive cerebral infaction.The progressive cerebral infarction patients were divided into 3 groups according to Pullicino's expressions:the big infarction focus(32 patients) ,the medium-sized infarction focus(34 patients) and the small infarction focus(36 patients) .The neurological deficits were divided into 3 groups according to the crrteria set by the 4th national cerebrovascular disease conference in 1995:light-defictits(38 patients),the moderate dificits (32 patients) and sever ditlcits (32 patients).The 65 non-progressive irffarction patients were stable without headache,vertigo and tinnitus.Arother 60 healthy subjects were entered as control group.Blood samples of all the patients' were collected at 0 h,24 h,3 d,7 d,14 d,24 d and the serum VE-eadherin by ELISA method was asaayed.All the data were analyzed by SPSS 10.0 software and One-way ANOVA was applied to intergroup comparisons for mote than two groups.Results The VF-cadherin level of patieras with progressive infarction increased in acute stage,reached the peak 3 days after onset,declined remarkably 7 days later and got nearly normalized within 21 days.The results were significantly different from those of non-progressive and controlgroup(P＜0.01).The VE-cadherin concentration was higher in patients with bigger size infarction and more sever symptoms.Conclusions The VE-cadherin level is related to the infarction size,course and the severity,and higher in the progressive group.VE-cadherin could be used for predicting prognostic of cerebral infarction and clinically valuable for treating ischemie cerebrovascular disease.

Objective To observe the dynamic changes of high-sensitive C-reactive protein (hs-CRP) and visfatin in patients with acute traumatic injury of brain. Method A total of 120 patients with equal number in each gender ( n = 60) and with average age of (43.2 ± 6.2) years were admitted and treated by the neurosurgical department of ICU from August 2009 to June 2010. All patients were eligible to the diagnostic criteria of craniocerebreal injury. The clinical conditions of patients were assessed with Glasgow coma scale (C CS) at admission,and as per the scores of GCS, the patients were classified into mild degree (13- 15, n = 40), moderate degree (9 - 12, n = 40) and severe degree (3 - 8, n = 40). Another 60 subjects from those asking for health care by physical examination as control with equal number in each gender and their average age was (42.2±6.7) years.Blood samples were collected from fasted patients within 12 hours, 1d, 3d, 7d and 15 days after admission, and the levels of hs-CRP and visfatin in peripheral blood were detected. Results The levels of hs-CRP and visfatin were significantly higher in brain injury group than those in control group on the admission day (both P ＜ 0.01 ),and they both had positive relationships with severity of injury. The level of hs-CRP increased to peak on the first day of admission and visfatin increased to the peak on the 3rd day after admission. There was a correlation between levels of hs-CRP and visfatin ( r = 0.63, P ＜ 0.01 ). Conclusions hs-CRP and visfatin levels are related to the severity of acute traumatic injury of brain.

One hundred and two pregnant women with obstructive sleep apnea hypopnea syndrome (OSAHS) and 36 healthy pregnant women( control group)were enrolled in the study.Serum visfatin and macrophage migration inhibitory factor (MIF) levels were detected by enzyme linked immunosorbent assay (ELISA).The results showed that apnea-hypopnea index (AHI),mean arterial pressure(MAP),serum visfatin and MIF levels in pregnant women with OSAHS were significantly higher than those of controls ( all P＜ 0.01 ). In pregnant women,AHI,serum visfatin and MIF levels of patients having OSAHS with hypertension were significantly higher than those of patients with OSAHS only (all P ＜ 0.01 ).Patients with severe OSAHS had higher levels of visfatin and MIF than those with moderate OSAHS,and patients with moderate OSAHS had higher visfatin and MIF levels than those with mild OSAHS ( P ＜0.01 ).The AHI was significantly correlated with MAP,serum levels of MIF and visfatin ( r =0.75,0.71,0.68,respectively,all P ＜0.01 ) in pregnant women with OSAHS.

Objective To investigate the serum levels of resistin after coronary stent implantation(CSI).Methods Forty patients who underwent coronary stenting were included in this study and another 40 patients who received coronary angiography during the same period were selected as control.The concentration of resistin and high-sensitivity C-reactive protein(Hs-CRP) was measured by enzyme-linked immunosorbent assay(ELISA).Blood samples for measurement of resistin and Hs-CRP were taken right before the procedure and at 1 h,6 h and 24 h after the procedure.Results The serum resistin level measured at 6 hr after the procedure in patients who had received PCI was higher than that before the procedure(30.4?9.2 ?g/L vs 23.4?6.4 ?g/L,t=9.03,P0.05).Conclusion Coronary stenting causes a detectable increase in serum levels of resistin and it can be regarded as an indicator for injury caused by coronary stenting.