Objective To study on the correlation of VEGF,HSP 70,NBNA score and neonatal asphysia. Methods Using ELISA to detect the expression level of VEGF,HSP 70 in mild asphyxia group,severe asphyxia group and control group. The expression level of VEGF ,HSP 70and the correlation between NBNA score and asphyxia were compared. Results The expression levels of VEGF ,HSP 70 in both mild and severe asphyxia groups were significantly statistically higher than the control group(P<0.05). The severe asphyxia group had even higher VEGF,HSP 70 levels than the mild asphyxia group(P<0.05).The expression level of VEGF,HSP 70 are negatively correlated with the 7 th,14 th,28th-day NBNA score (r=-0.712,-0.629,-0.493;r=-0.621,-0.506,-0.016 ;P < 0.05).The area curve(AUC)for VEGF,HSP 70 leves to predicted respectively were 0.873 (95%CI 0.771~0.892,P<0.05)and 0.815(95%CI 0.729~0.871,P<0.05). Conclusions VEGF,HSP 70 lev-els in umbilical cord blood are closely related to neonatal asphyxia.The more severe the asphyxia ,the higher the expression level of VEGF and HSP 70,and the lower the NBNA score.

Objective To analyze the correlation of inflammatory markers C-reactive proten(CRP),serum amyloid protein A(SAA),interleukin-6(IL-6)levels and subjective sleep characteristics in patients with first-episode acute cerebral infarction.Methods A total of 113 patients with first-episode cerebral infarction admitted to the Department of Neurology,the People's Hospital of Fuyang from March 2022 to April 2023 were prospectively and continuously selected as subjects.According to the Pittsburgh sleep quality index(PSQI),they were divided into insomnia group(PSQI＞7 points)and non-insomnia group(PSQI ≤7 points).General demo-graphic data and differences in CRP,SAA,IL-6 levels,Hamilton anxiety scale(HAMA)and Hamilton depression scale(HAMD)scores were compared between the two groups.Partial correlation analysis was used to analyze the correlation between three serum markers and PSQI effect factors.Results There were no significant differences in age,gender,baseline NIHSS score and mRS score between the two groups(P＞0.05).HAMD scores(z=-3.993,P＜0.001),HAMA scores(z=-3.806,P＜0.001),CRP,IL-6,SAA(P＜0.001)in insomnia group were significantly higher than those in non-insomnia group.The history of hyperlipidemia between the two groups was statistically significant(z=5.913,P=0.015).Multivariate Logisitic regression analysis showed that CRP(OR=1.55,P＜0.01),HAMD scores,HAMA scores and hyperlipidemia were independent risk factors for chronic insomnia in patients with first-episode cerebral infarction,and HAMD scores had a greater effect than HAMA scores(OR:1.10 vs 1.04).Partial correlation analysis showed that IL-6 and CRP levels were significantly correlated with the total score of PSQI(P＜0.05),while SAA was not significantly correla-ted with the total score of PSQI(P＞0.05).IL-6 level was positively correlated with sleep quality(r=0.231)sleep efficiency(r=0.322)and sleep duration(r=0.221).SAA level was positively correlated with sleep efficiency(r=0.242),while CRP level was posi-tively correlated with sleep latency(r=0.194),sleep duration(r=0.247)and sleep efficiency(r=0.225).Conclusion The inflam-matory markers CRP,IL-6 and SAA levels were elevated in the patients with first-episode cerebral infarction accompanied by insomnia,which were correlated with the severity of insomnia.The correlation between CRP and IL-6 levels and sleep characteristics was consistent with each other.

Objective:To study the effect of melatonin (MEL) on the pyroptosis of hippocampus in neonatal rats with hypoxic-ischemic brain damage (HIBD), and the related mechanism.Methods:The animal model of HIBD was established by the modified Rice method.According to the random number table, a total of 105 Sprague-Dawley (SD) rats aged 7 days were divided into 7 groups (15 rats in each group): sham operation (Sham) group, model (HIBD) group, MEL treatment group (5, 10 and 20 mg/kg), phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) pathway inhibitor (LY294002) treatment group and MEL+ LY294002 group.The hippocampus neuronal morphology and the changes of nissl bodies were observed through HE staining and nissl staining.The mRNA expression levels of Nod-like receptor family 3 (NLRP3), apoptosis-associated speck-like protein containing a card (ASC), Caspase-1, gasdermin D (GSDMD), interleukin-1β (IL-1β) and interleukin-18 (IL-18) in the left hippocampus of rats were detected by real-time fluorescence quantitative PCR.The protein expression level of the above indexes and the level of phosphorylated Akt (p-Akt) were measured by Western blot.Results:Compared with the Sham group, the number of cell layers in hippocampal CA1 region in the HIBD group decreased, the cell arrangement was irregular, and there were less nissl bodies.Besides, the mRNA expression levels of NLRP3 (1.98±0.08 vs.0.86±0.13), ASC (1.40±0.12 vs.0.81±0.07), Caspase-1 (1.46±0.10 vs.0.75±0.09), GSDMD (1.35±0.10 vs.0.81±0.10), IL-18 (1.23±0.08 vs.0.23±0.04), IL-1β (1.83±0.09 vs.0.57±0.08) and p-Akt (1.12±0.12 vs.0.54±0.07) in the HIBD group were significant higher than those in the Sham group (all P<0.05). Compared with the HIBD group, there were more cell layers in hippocampal CA1 region of the MEL group (10 mg/kg), the arrangement of cells was more regular, and the number of nissl bodies increased.The mRNA expression levels of NLRP3 (1.04±0.10), ASC (0.91±0.06), Caspase-1 (0.63±0.06), GSDMD (1.01±0.09), IL-18 (0.65±0.05) and IL-1β (0.63±0.10) in the MEL group were statistically significantly lower than those in the HIBD group (all P<0.05). Compared with the MEL group (10 mg/kg), the arrangement of cells in hippocampal CA1 region of the MEL+ LY294002 group was relatively disordered, the nissl bodies declined, the p-Akt protein level (0.87±0.09 vs.1.99±0.27) decreased significantly, and the Caspase-1(p20) protein level (0.85±0.09 vs.0.58±0.09) increased significantly (all P<0.05). Conclusions:MEL may inhibit the hippocampal pyroptosis in neonatal rats with HIBD by activating the Akt signaling pathway, thereby protecting the brain.

OBJECTIVE: To investigate the formation of gap junctions between Schwann cells derived from differentiated adipose stem cells implanted in a rat model of dyskinesia induced by brain injury and its positive effect in promoting functional recovery of the rats. METHODS: In a rat model of hemiplegia induced by motor cortex injury, adipose stem cells or Schwann cells differentiated from adipose stem cells, either with or without RNAi-mediated silencing of Cx43, were transplanted orthotopically in the lesion. The recovery of the motor function of the rats was observed and scored after the transplantation. Rat brain tissues were sampled to detect the expressions of nerve growth factor (NGF) using Western blotting and RT-PCR. RESULTS: All the 3 cell transplantation therapies obviously improved the motor function scores of the rats as compared with the control rats. The expression of NGF in the brain tissue was significantly lower in the control group than in the cell transplantation groups. NGF expression in the brain tissues of rats receiving transplantation of Schwann cells with Cx43 gene silencing was lower than that in rats receiving Schwann cells without Cx43 silencing, and was similar with that in rats transplanted with adipose stem cells. The results of RT-PCR showed that NGF mRNA level in the control group was significantly lower than that in the other 3 groups. NGF mRNA expression was the highest in Schwann cell group without Cx43 silencing, followed by adipose stem cell group, and then by Schwann cell group with Cx43 silencing. CONCLUSIONS In the rat model of dyskinesia induced by brain injury, transplantations of adipose stem cells and adipose stem cells-derived Schwann cells both promote the functional recovery of brain damage, in which gap junction protein Cx43 plays an important role to promote functional gap junction formation possibly by enhancing NGF expression.
Animals ; Brain Injuries ; Dyskinesias ; Gap Junctions ; Rats ; Rats, Sprague-Dawley ; Schwann Cells ; Stem Cells

Objective:To systematically evaluate the effect of different pressing time on bleeding in patients with subcutaneous injection of low molecular weight heparin (LMWH) .Methods:We retrieved the randomized controlled trial (RCT) or controlled clinicial trials (CCT) on local pressing time and bleeding in patients with subcutaneous injection of LMWH by searching PubMed, Embase, Cochrane Library, China National Knowledge Infrastructure (CNKI) , China Biology Medicine (CBM) , Wanfang Data and VIP from the database establishment to September 2019. Two researchers independently screened literature based on inclusion and exclusion criteria, evaluated quality and extracted data. RevMan 5.3 was used to conduct Meta-analysis on the extracted data.Results:A total of 9 studies that met the criteria were finally included and the included documents were all in Chinese, of which 4 were RCTs and 5 were CCTs. Subcutaneous hemorrhage diameter <5 mm was defined as no bleeding, subcutaneous hemorrhage ≥5 mm was defined as bleeding, and bleeding incidence was used as the final outcome indicator. According to different pressing time, they were divided into non-pressing group, group of pressing for 3 minutes, group of pressing for 5 minutes and group of pressing for 10 minutes, and were compared each group in pairs. Meta-analysis results showed there were significantly statistical differences between non-pressing group and group of pressing for 3 minutes [ OR=0.44, 95% CI (0.25, 0.78) , P=0.005], between group of pressing for 3 minutes and group of pressing for 5 minutes [ OR=2.12, 95% CI (1.18, 3.83) , P=0.010]; there were no significantly statistical differences between non-pressing group and group of pressing for 5 minutes [ OR=0.94, 95% CI (0.32, 2.81) , P=0.920], between non-pressing group and group of pressing for 10 minutes [ OR=1.11, 95% CI (0.33, 3.70) , P=0.870], between group of pressing for 5 minutes and group of pressing for 10 minutes [ OR=2.56, 95% CI (0.72, 9.11) , P=0.150]. Conclusions:Subcutaneous injection of LMWH without pressing is better than pressing for 3 minutes, and pressing for 5 minutes is better than pressing for 3 minutes. Considering the limited number of included documents and the quality grades are all B, research conclusions still need to be verified by large samples and rigorous experimental design.

Objective: To explore the hemorrhagic transformation(HT) and its effect on prognosis in patients with acute ischemic stroke(AIS) after mechanical thrombectomy(MT). Methods: A total of 114 patients with AIS received MT were enrolled. The modified Rankin Scale was used to evaluate the clinical outcome at 90 days of onset(0-2 points were good prognosis; 3-6 points were poor prognosis). The patients were divided into HT group(n=25) and non-HT group(n=89) according to their HT conditions. Binomial Logistic regression analysis was performed to determine the vascular risk factors of HT after MT and the effect of HT on prognosis. Results: Among 114 patients, there were 25 cases of HT and 89 cases of non HT. The proportion of patients with diabetes in HT group was significantly higher than that in non-HT group. The NIHSS score of HT group at discharge was significantly higher than that in non-HT group. The proportion with good prognosis at 90 days in HT group was significantly lower than that in non-HT group(allP<0.05). Binomial Logistic regression analysis showed that diabetes, high levels of cholesterol and smoking were the major vascular risk factors for HT after thrombectomy(allP<0.05). HT was an important factor affecting the poor prognosis after arterial thrombectomy(P=0.026). Conclusion Diabetes, high levels of cholesterol and smoking are the main risk factors of HT after MT for AIS. HT is an independent risk factor for poor prognosis after MT.