1.Application of big data in medical care and their privacy protection
Youjiang HUANG ; Lian HE ; Huanqun SU ; Lirong ZHENG
Chinese Journal of Medical Library and Information Science 2015;(9):43-45
Big data can provide effective data support for clinical research, biomedical technology progress, and individualized treatment of patients.However, medical ethics and data privacy must be considered due to the poor relation between patients and medical workers .The advantages and disadvantages of big data in medical practice were thus analyzed with certain strategies proposed for their development and privacy protection , and for the balance between the benefits and risks of technological innovations .
2. Analysis of difference in oral bacteria flora in saliva among elderly people in longevity zone of Guangxi
Yanchun QIN ; Yanqiang HUANG ; Huaying TANG ; Ganrong HUANG ; Hongyu WEI ; Zhenfeng XIE ; Gang LU
Chinese Journal of Geriatrics 2020;39(1):78-82
Objective:
To investigate the bacterial species, characteristics and differences of oral bacteria flora of saliva in the longevous elderly between in Bama county and in Debao county in Guangxi, in order to explore the relationship between longevity and oral salivary bacteria flora in the elderly.
Methods:
The saliva was taken from the longevous elderly in Bama county(BM group)and people aged over 60 years in Debao county(BS group)separately, and the total DNA was extracted.The 16S rDNA-V4 region was amplified by PCR and analyzed by sequencing.The main species and diversity of bacterial colonies were recorded for difference analysis.
Results:
A total of 14 saliva samples were collected from 7 cases in BM group and 7 cases in BS group.A total of 369 OTUs were generated by cluster analysis of 14 samples.At the genus level, the dominant salivary bacteria flora were
3.Expression and role of the TRPC family in TGF-β1-induced calcium influx in podocytes.
Hai-Ting HUANG ; Xu LIN ; Peng-Wei GUO ; Jun PANG ; Jing MA ; Lin-Lin HE ; Xin-Tong ZHENG
Acta Physiologica Sinica 2022;74(6):1005-1013
The TRPC family consists of multiple important cationic channels in mammals that participate in a variety of physiological and pathological processes. Our previous studies have shown that transforming growth factor-β1 (TGF-β1) increases the expression of TRPC6 in podocytes, but the roles of other members of the TRPC family in podocytes require further investigation. In this study, we investigated the effect of TGF-β1 on the expression of the TRPC family and the role of the TRPC family in the changes of the intracellular Ca2+ concentration ([Ca2+]i) in podocytes induced by TGF-β1. The model of podocyte injury was established by treatment with TGF-β1 in immortalized glomerular podocytes (MPC5) in vitro. qRT-PCR and Western blot were used to detect the effect of TGF-β1 on the mRNA and protein expression of each TRPC family member. After the expression of each TRPC family member was knocked down by a siRNA-based approach and blocked by SKF96365, respectively, free cytosolic Ca2+ was measured using the fluorescent Ca2+ indicator Fluo-3/AM, and the dynamic change of [Ca2+]i in podocytes was detected by a dynamic high-speed calcium imaging system. The results showed that TGF-β1 increased the protein expression of TRPC1/3/6 in podocytes, but had no effects on the protein expression of TRPC4. The protein expression levels of TRPC5/7 were only affected by 4 ng/mL and 8 ng/mL TGF-β1, respectively. TGF-β1 increased TRPC1/3/6 mRNA levels in podocytes, however had no effects on TRPC4/5/7 mRNA. TGF-β1 significantly increased [Ca2+]i in podocytes. Knockdown of TRPC1/4/5/7 in podocytes had no significant effect on the [Ca2+]i induced by TGF-β1, but TRPC3/6 knockdown significantly decreased the [Ca2+]i. There was no significant difference in the [Ca2+]i between the TRPC6 siRNA-treated group and SKF96365-treated group, but the [Ca2+]i of the TRPC3 siRNA-treated group was significantly higher than that of SKF96365-treated group. These results demonstrate that TGF-β1 increases the expression of the TRPC1/3/6 in podocytes. TGF-β1 increases [Ca2+]i in podocytes, which is dependent on the TRPC3/6 expression. Our results also suggest that the effect of TRPC6 on [Ca2+]i in podocytes may be greater than that of TRPC3.
Animals
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TRPC6 Cation Channel/metabolism*
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Calcium/metabolism*
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TRPC Cation Channels/metabolism*
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Podocytes/metabolism*
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Transforming Growth Factor beta1/metabolism*
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RNA, Small Interfering/metabolism*
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RNA, Messenger/metabolism*
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Mammals/metabolism*
4.Effect of knockdown IGSF10 on proliferation,migration and invasion capacity of lung adenocarcinoma cells
Lianyu Cheng ; Beibei Ma ; Yu Huang ; Yanli Li ; Zhongwei Zhang ; Guangbin Ye ; Bo Ling
Acta Universitatis Medicinalis Anhui 2024;59(1):45-51
Objective :
To investigate the effects of immunoglobulin gene superfamily 10 (IGSF10) on prolifera- tion,migration and invasion of lung adenocarcinoma cells.
Methods :
ioinformatics was applied to study the ex- pression levels of IGSF10 in tumor tissues and normal tissues. Western blot and quantitative real-time PCR ( qPCR) were used to detect the expression level of IGSF10 in lung adenocarcinoma cell lines and normal lung epi- thelial cells.Knockdown of IGSF10,the effect of knockdown of IGSF10 on proliferation,migration and invasion of lung adenocarcinoma A549 cells was examined using cell counting kit-8 ( CCK-8) ,Transwell migration and inva- sion assay,scratch assay and plate cloning assay.The effects of knockdown of IGSF10 on the expression of invasion and migration-related genes in A549 cells were examined by Western blot and qPCR assays.
Results :
IGSF10 ex- pression in lung adenocarcinoma tissues was lower than that in normal tissues (P <0. 05) .IGSF10 expression in lung adenocarcinoma cell lines was lower than that in lung epithelial cells (P<0. 05) .Knockdown of IGSF10 pro- moted the ability of lung adenocarcinoma A549 cells to proliferate ,proliferation ,migration and invasion ( P < 0. 05) .Knockdown of IGSF10 promoted the expression of regulatory epithelial-mesenchymal transition marker Neu- ral-cadherin (N-cadherin) and key transcription factors Snail family transcriptional repressor 1 (Snail) and Snail family transcriptional repressor 2 (Slug) (P<0. 05) and inhibited the expression of Epithelial-cadherin (E-cad- herin) (P<0. 05) .
Conclusion
Knockdown of IGSF10 may promote proliferation,migration and invasion of lung adenocarcinoma cells through activation of Snail,Slug / E-cadherin signaling axis,and this result may provide a po- tential new target for clinical diagnosis and treatment of lung adenocarcinoma.
5.Thoughts on establishing a medical continuing education online platform based on the libraries and academic conferences
Youjiang HUANG ; Lishi ZENG ; Lirong ZHENG ; Lian HE ; Huanqun SU ; Haiteng MA ; Zhuoyong HUANG
Chinese Journal of Medical Education Research 2021;20(2):233-236
This study explores the construction and application of online platform for medical continuing education based on libraries and academic conferences. With the consent of the experts participating in the conference, the contents of the conferences are recorded and made into learning videos for continuing education. By constructing network servers, cloud services, and other platforms, the online continuing education can be realized, and such procedures as giving credits also can be completed. And combined with other professional continuing education platforms, a complete continuing education system can be constructed. We have initially designed out the construction mode of medical continuing education platform. The construction of medical continuing education platform has enhanced the class or position of the symposium organization institutions, facilitated the continuing education of professionals and technical personnel, with good social and economic effects, which is worthy of promotion.
6.Study on in vitro Antioxidant Activities of Different Polar Parts of Ethanol Extract from Amomum tsao-ko
Shimei CHEN ; Biyi HUANG ; Suoyi HUANG
China Pharmacy 2020;31(8):953-956
OBJECTIVE:To evaluate in vitro antioxidant activities of 4 different polar parts of ethanol extract from Amomum tsao-ko,and to lay a foundation for the research and development of antioxidant chemical components in the plant. METHODS : The dried fruits of A. tsao-ko were crushed ,then were hearted and reflux extracted with 95% ethanol. The extraction fluid was concentrated by rotary evaporation and evaporated in water bath to obtain the ethanol extract. The extract was dispersed in water , and then extracted with petroleum ether ,ethyl acetate and n-butanol organic solvents one by one. Each solvent extract was combined and the lower water phase were collected. Finally ,the petroleum ether part ,ethyl acetate part ,n-butanol part and water part were obtained,after rotary evaporation concentration and water bath evaporation. Through in vitro antioxidant activity tests ,using 2, 6-di-tert-butyl-4-methylphenol(BHT)as positive control ,DPPH radical ,superoxide anion radical scavenging ability and Fe 3+ reducing ability of different polar parts of ethanol extract from A. tsao-ko were investigated. RESULTS :The scavenging rates of 4 polar parts of ethanol extract from A. tsao-ko on DPPH radical were all over 80%;the order of scavenging ability was ethyl acetate part>BHT>n-butanol part >petroleum ether part >water part. Those of the 4 polar parts to superoxide anion radical were between about 30%-40% mostly;the order of scavenging ability was n-butanol part >petroleum ether part >water part >ethyl acetate part > BHT;but those were weaker than their scavenging ability to DPPH r adical. The polar parts of ethanol extract also had a certain reduction ability to Fe 3+;the order of the reduction ability was n-butanol part >BHT>ethyl acetate part >petroleum ether part > water part on the whole ,but that of water part rose to the stron- gest when its concentration was 4.0 μg/mL. CONCLUSIONS: The different polar parts of ethanol extract from A. tsao-kom have certain in vitro antioxidant capacity ,but the order of antioxidant activity of different polar parts was not the same in different antioxidant activity tests ;ethyl acetate part has the 163.com strongest scave nging effect on DPPH radical ,n-butanol part has the strongest scavenging ability on superoxide anion radical and reducing ability on Fe 3+.
8. Distribution characteristics of polymorphism of miRNA-107 gene in Guangxi population and their association with serum lipid levels
Yan-Yun HUANG ; Hua-Tuo HUANG ; Yu-Lan LU ; Ming LEI ; Tan TAN ; Ye-Sheng WEI ; Yan-Yun HUANG
Acta Anatomica Sinica 2020;51(2):284-288
Objective To study the distribution characteristics of single nucleotide polymorphisms (SNP) of miR-107 gene rs2296616 C/T in Guangxi healthy population and comparison with that in different ethnic populations, and further to explore the correlation between rs2296616 C/T SNP and blood lipid level. Methods The polymorphisms of miR-107 gene rs2296616 C/T among 372 Chinese healthy individuals of Guangxi were detected by multiplex SNaPshot and DNA sequencing method, and the blood lipid-related indexes were detected by 7600 biochemical analyzer. The distribution of rs2296616 C/T polymorphism among different ethnic groups and the differences of blood lipid levels among different genotypes were compared by statistical method . Results MiR-107 gene rs2296616 C/T SNP contained TT(91. 1%), CT (8. 9%)genotypes and T(95. 6%), C(4. 4%)alleles in Guangxi healthy population. The frequencies of genotype and allele distribution of rs2296616 C/T were not significantly different among genders in Guangxi population(P>0. 05). However, there were significant differences in the genotype and allele frequency of miR-107 gene rs2296616 C/T in Guangxi healthy population compared with those of Europeans, Japanese, Africans, Mexicans and Indians published in HapMap(P<0. 05), no significant difference compared with HapMap-HGB (P > 0. 05). When compared the blood lipid level among two genotypes in rs2296616 C/T, we found that the level of high density lipoprotein cholesterol(HDL-C) with TT genotype was significantly different from that of CT group (P < 0. 05) . Conclusion There are different degrees of variation in the polymorphisms of rs2296616 C / T of miR-107 gene between Guangxi people and other ethnic populations. The polymorphism of rs2296616 C / T locus is related to the level of HDL-C.
9.Improvement of detection of paternally inherited fetal mutant genes for β-globin in maternal plasma by PNA clamp.
Chinese Journal of Hematology 2013;34(3):233-236
OBJECTIVETo study improvement of detection of paternally herited fetal mutant genes for β-globin in maternal plasma by PNA clamp to seek a noninvasive prenatal diagnostic method for β-thalassemia.
METHODSA total of 38 maternal blood samples were collected at 7 to 20 weeks of gestation, samples in which the father carried CD41-42 mutation and mother carried normal gene or the other point mutation for β-thalassemia were examined. The results of fetal DNA in amniotic fluid, cord blood or peripheral blood of newborns were used as the gold standard for comparison. In the study group, the total cell-free DNA was extracted from maternal plasma using QIAamp DNA Blood Mini Kit. After extraction, the total cell-free DNA was separated by agarose gel (1%) electrophoresis, and the cell-free DNA with a size of 100-300 bp was retrieved from the gel slice. Then, the retrieved DNA-free cell underwent PCR amplified with a PNA clamp. The genotype was confirmed by the conventional method (reverse dot blot hybridization), and the results were compared to gold standard. Simultaneously, two control groups with different PCR procedures were set up. The PCR procedure of control group A was amplified with the extracted total cell-free DNA and PNA clamp, and the PCR procedure of control group B was amplified with the retrieved size-fractionated DNA-free cell without PNA clamp.
RESULTSPlasma samples from 38 pregnant women were detected using PCR products for hybridization, the results were compared with the gold standard. Regarding the 21 samples confirmed by gold standard with fetal genotype 41-42M/N, 19, 8, 12 cases were detected as fetal genotype 41-42M in study group, control group A and control group B respectively, the sensitivity was 90.5% (19/21), 38.1% (8/21) and 57.1% (12/21) respectively;Concerning the 17 samples confirmed by gold standard with fetal normal genotype, the amount of false positive cases were 1, 2 and 1 respectively. The respective specificity was 94.1% (16/17), 94.1% (16/17) and 88.2% (15/17) respectively. The respective accuracies were 92.1% (35/38), 63.2% (24/38) and 71.1% (27/38) respectively. The difference in sensitivity and specificity was pairwise compared by means of McNemar's test. There was significant difference between new study group and control group A or control group B (all P﹤0.05).
CONCLUSIONThe method of detection of paternally inherited fetal mutation genes for β-thalassemia using small size of fetal DNA-free cell in maternal plasma with PNA clamp had several advantages of reliable sensitivity, specificity and accuracy, indicating its potential of clinical practicality.
Adolescent ; Adult ; DNA ; blood ; Female ; Humans ; Inheritance Patterns ; Mutant Proteins ; genetics ; Mutation ; Peptide Nucleic Acids ; Pregnancy ; Prenatal Diagnosis ; Young Adult ; beta-Globins ; genetics ; beta-Thalassemia ; diagnosis ; genetics
10.Polymorphism distribution of 5bp insertion/deletion in promoter region of LncRNA [STHX] GAS5 gene in population of Guangxi district
Chunhong LIU ; Yulan LU ; Huatuo HUANG ; Yan WANG ; Chunfang WANG ; Yan LAN ; Yesheng WEI
Chinese Journal of Clinical Laboratory Science 2019;37(8):603-607
Objective:
To investigate the characteristics of polymorphism distribution at the functional insertion/deletion locus rs145204276I/D in the promoter region of LncRNA GAS5 (growth-arrest specific transcript 5) gene in population of Guangxi district, and analyze the differences of polymorphism distribution of rs145204276I/D in the populations between Guangxi and other regions.
Methods:
SNPscan high-throughput sequencing technique was used to detect rs145204276I/D locus in genotype of GAS5 gene of 289 subjects from Guangxi district, and the distribution frequencies of genotypes and alleles between different genders were analyzed. The differences of polymorphism distribution were compared with those in the database from the population of European (EUR), Japanese in Tokyo (JPT), South Asian (SAS), Admixed American (AMR), African (AFR), Chinese Han in Beijing (CHB), Nanjing, Jilin, Chongqing and Kunming which were published by 1000 genome project or reported in literatures.
Results:
The frequencies of I/I, I/D and D/D genotypes of rs145204276I/D in GAS5 were 48.4%, 43.6% and 8.0%, respectively. The frequencies of I and D alleles were 70.2% and 29.8%, respectively. No significant difference of genotype and allele frequencies of rs145204276I/D was observed between different genders in Guangxi population ( P >0.05). The genotype and allele frequencies of rs145204276I/D in Guangxi population were significantly different from JPT, EUR, AFR, SAS and AMR populations ( P <0.05), but were not significantly different from those of Chinese Han population in Beijing, Nanjing, Jilin, Chongqing and Kunming ( P >0.05).
Conclusion
The distribution of LncRNA GAS5 gene rs145204276I/D polymorphism in Guangxi population was not different between men and women, and the polymorphism of LncRNA GAS5 gene was different from those of other regions in the world.